RLG00000007230

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012310
Physical Location & Seq
Forward (+)
14761907 .. 14764614
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007230

Sequence Viewer

Length: 987 bp
ATGTCCGCCTTATCGAGAGGCCTCATTTCCCGCCTCCGCCTCCTCTCCCTCAACCCCGCCACTTCTTCTTCCTCCTCTTCTACTTGTACCGCTATACCCGCCGCTCACGCTACGACGTCGTATTCTCATTTCTTCCGATGCTTCAGCTCCGAGGCCTTGGTGGACGGGGCCGAGATCGCCGAAGATCGCAGCCGGGTTATCGAGGCCAAGCCCGGAGTTATGACCCCCCAATCGAAGCGGACCGGAGTCATCGCCGTCAAGTGTGGCATGTCGGCGCGCTGGGACAAATGGGGTGCTAGGGTTCCGATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTCAAAACCCCAGAGAAAGAAGGAATCTCTGCCCTACAGATTGGGTGCGGGCAGAAAAAAGCAAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAGGGTGTTCCACTTAAGAGGAAACTGCGAGAGTTTCCTGTAACAGAAGATGCGCTTCTCCCTGTTGGTATGTCAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTTACAGGAATCACGAGAGGGAAAGGTTTTCAGGGTGGGATGAAAAGGTATGGTTTTAGAGGAATGCCAGCAAGTCATGGTGCTTCTAAGTCACACCGGAGTATTGGTTCTACAGGTCAGAGAGATGATGCTGGAAGGGTCTTTAAAGGCAGAAAGATGCCCGGGCGCATGGGTGGAAAACAGAGAACGGTGAAAAATGTATGGATCTACAAAATTGATCCCGCAAGGAACTTGATATGGGTGAGAGGCCAGGTCCCAGGTGCTGAAGGGAACTTTGTGTTTATCAAGGATGCTTTCTACAAGAAACCCGATGTCTCGATGCTACCATTTCCAACTTACTTTGCTCCAGAGGATGAAGATCCAAGCAAGTTAGAACCGCTGGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGCGGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

329

Amino Acids

35.8

Weight (kDa)

9.84

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 178 - 261 7.8e-17 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 104
AccII CGCG 1 cut(s) 277
AclWI GGATC 4 cut(s) 773, 773, 914, 959
AcuI CTGAAG 3 cut(s) 127, 447, 846
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 318, 431
AflII CTTAAG 1 cut(s) 461
AjnI CCWGG 3 cut(s) 544, 810, 817
AloI GAACNNNNNNTCC 2 cut(s) 652, 684
AluBI AGCT 2 cut(s) 147, 446
AluI AGCT 2 cut(s) 147, 446
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 2 cut(s) 343, 880
AlwI GGATC 4 cut(s) 773, 773, 914, 959
AlwNI CAGNNNCTG 1 cut(s) 824
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 7 cut(s) 19, 153, 168, 204, 433, 547, 808
ApeKI GCWGC 1 cut(s) 189
AspLEI GCGC 4 cut(s) 277, 279, 502, 729
AspS9I GGNCC 4 cut(s) 168, 240, 433, 814
AsuC2I CCSGG 4 cut(s) 194, 213, 723, 724
AsuHPI GGTGA 3 cut(s) 301, 763, 814
AvaI CYCGRG 1 cut(s) 722
AvaII GGWCC 2 cut(s) 240, 814
BanII GRGCYC 1 cut(s) 448
BauI CACGAG 1 cut(s) 574
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 1 cut(s) 201
BceAI ACGGC 1 cut(s) 239
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 3 cut(s) 546, 812, 819
BcnI CCSGG 4 cut(s) 194, 213, 723, 724
BcoDI GTCTC 2 cut(s) 343, 880
BfaI CTAG 1 cut(s) 297
BfmI CTRYAG 2 cut(s) 380, 672
BfrI CTTAAG 1 cut(s) 461
BisI GCNGC 3 cut(s) 102, 190, 978
BlsI GCNGC 3 cut(s) 103, 191, 979
Bme1390I CCNGG 7 cut(s) 194, 213, 546, 723, 724, 812, 819
Bme18I GGWCC 2 cut(s) 240, 814
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 4 cut(s) 168, 240, 433, 814
BmiI GGNNCC 3 cut(s) 169, 303, 816
BmrFI CCNGG 7 cut(s) 194, 213, 546, 723, 724, 812, 819
BmsI GCATC 6 cut(s) 128, 487, 679, 708, 841, 870
BoxI GACNNNNGTC 1 cut(s) 245
BpmI CTGGAG 1 cut(s) 891
Bpu10I CCTNAGC 1 cut(s) 447
BpuMI CCSGG 4 cut(s) 194, 213, 723, 724
BsaBI GATNNNNATC 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 4 cut(s) 150, 156, 722, 817
BsaWI WCCGGW 2 cut(s) 242, 657
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 431
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 3 cut(s) 546, 812, 819
BseDI CCNNGG 4 cut(s) 150, 156, 722, 817
BseGI GGATG 3 cut(s) 606, 856, 919
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 2 cut(s) 318, 431
BseMII CTCAG 2 cut(s) 356, 461
BsePI GCGCGC 1 cut(s) 275
BseRI GAGGAG 2 cut(s) 32, 64
BseXI GCAGC 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 7 cut(s) 21, 155, 170, 206, 435, 549, 810
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 722
BsiSI CCGG 5 cut(s) 193, 213, 243, 658, 723
BslFI GGGAC 2 cut(s) 296, 800
BslI CCNNNNNNNGG 2 cut(s) 318, 431
BsmAI GTCTC 2 cut(s) 343, 880
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 2 cut(s) 296, 800
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 7 cut(s) 21, 155, 170, 206, 435, 549, 810
BsoBI CYCGRG 1 cut(s) 722
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
BspANI GGCC 7 cut(s) 21, 155, 170, 206, 435, 549, 810
BspCNI CTCAG 2 cut(s) 355, 460
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 169, 303, 816
BspPI GGATC 4 cut(s) 773, 773, 914, 959
BspTI CTTAAG 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 104
BssECI CCNNGG 4 cut(s) 150, 156, 722, 817
BssHII GCGCGC 1 cut(s) 275
BssMI GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
BssNI GRCGYC 1 cut(s) 116
BssSI CACGAG 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 1 cut(s) 574
Bst2UI CCWGG 3 cut(s) 546, 812, 819
Bst4CI ACNGT 2 cut(s) 313, 751
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 116
BstAFI CTTAAG 1 cut(s) 461
BstC8I GCNNGC 3 cut(s) 277, 395, 630
BstDEI CTNAG 3 cut(s) 342, 447, 648
BstF5I GGATG 3 cut(s) 606, 856, 919
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 4 cut(s) 277, 279, 502, 729
BstKTI GATC 8 cut(s) 177, 187, 309, 768, 781, 922, 952, 967
BstMAI GTCTC 2 cut(s) 343, 880
BstMBI GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
BstMWI GCNNNNNNNGC 3 cut(s) 98, 107, 176
BstNI CCWGG 3 cut(s) 546, 812, 819
BstNSI RCATGY 1 cut(s) 271
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 7 cut(s) 192, 211, 544, 721, 722, 810, 817
BstSFI CTRYAG 2 cut(s) 380, 672
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 1 cut(s) 201
BstX2I RGATCY 3 cut(s) 765, 919, 964
BstYI RGATCY 3 cut(s) 765, 919, 964
BsuRI GGCC 7 cut(s) 21, 155, 170, 206, 435, 549, 810
BtgZI GCGATG 1 cut(s) 235
BtsCI GGATG 3 cut(s) 606, 856, 919
Cac8I GCNNGC 3 cut(s) 277, 395, 630
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 4 cut(s) 277, 279, 502, 729
Cfr13I GGNCC 4 cut(s) 168, 240, 433, 814
Cfr9I CCCGGG 1 cut(s) 722
CpoI CGGWCCG 1 cut(s) 240
Csp6I GTAC 1 cut(s) 87
CspI CGGWCCG 1 cut(s) 240
CviAII CATG 3 cut(s) 268, 638, 730
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 3 cut(s) 342, 447, 648
DpnI GATC 8 cut(s) 176, 186, 308, 767, 780, 921, 951, 966
DpnII GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
DraI TTTAAA 1 cut(s) 706
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 156
Eco147I AGGCCT 2 cut(s) 21, 155
Eco24I GRGCYC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 240, 814
Eco53kI GAGCTC 1 cut(s) 446
Eco57I CTGAAG 3 cut(s) 127, 447, 846
Eco88I CYCGRG 1 cut(s) 722
EcoICRI GAGCTC 1 cut(s) 446
EcoO109I RGGNCCY 1 cut(s) 814
EcoRII CCWGG 3 cut(s) 544, 810, 817
EcoT14I CCWWGG 1 cut(s) 156
EcoT38I GRGCYC 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 156
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 3 cut(s) 271, 641, 733
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 2 cut(s) 296, 800
FatI CATG 3 cut(s) 267, 637, 729
FauI CCCGC 5 cut(s) 38, 64, 106, 386, 790
Fnu4HI GCNGC 3 cut(s) 102, 190, 978
FokI GGATG 3 cut(s) 613, 863, 926
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 3 cut(s) 102, 190, 978
FspBI CTAG 1 cut(s) 297
GlaI GCGC 4 cut(s) 276, 278, 501, 728
GluI GCNGC 3 cut(s) 102, 190, 978
GsaI CCCAGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 891
HaeIII GGCC 7 cut(s) 21, 155, 170, 206, 435, 549, 810
HapII CCGG 5 cut(s) 193, 213, 243, 658, 723
HhaI GCGC 4 cut(s) 277, 279, 502, 729
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 3 cut(s) 271, 641, 733
Hin6I GCGC 4 cut(s) 275, 277, 500, 727
HinP1I GCGC 4 cut(s) 275, 277, 500, 727
HinfI GANTC 3 cut(s) 246, 369, 570
HpaII CCGG 5 cut(s) 193, 213, 243, 658, 723
HphI GGTGA 3 cut(s) 301, 763, 814
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 6 cut(s) 137, 151, 306, 417, 443, 681
Hpy188III TCNNGA 4 cut(s) 15, 574, 877, 908
Hpy8I GTNNAC 1 cut(s) 163
Hpy99I CGWCG 2 cut(s) 118, 121
HpyAV CCTTC 3 cut(s) 359, 690, 821
HpyCH4III ACNGT 2 cut(s) 313, 751
HpyCH4IV ACGT 1 cut(s) 116
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 107, 176
HpyF3I CTNAG 3 cut(s) 342, 447, 648
HpySE526I ACGT 1 cut(s) 116
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 3 cut(s) 271, 641, 733
HspAI GCGC 4 cut(s) 275, 277, 500, 727
Kzo9I GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
LmnI GCTCC 2 cut(s) 152, 910
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 6 cut(s) 128, 487, 679, 708, 841, 870
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 3 cut(s) 487, 562, 651
MalI GATC 8 cut(s) 176, 186, 308, 767, 780, 921, 951, 966
MbiI CCGCTC 1 cut(s) 104
MboI GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
MboII GAAGA 7 cut(s) 57, 60, 69, 124, 194, 506, 929
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 3 cut(s) 765, 919, 964
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 2 cut(s) 522, 774
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 1 cut(s) 917
MseI TTAA 2 cut(s) 462, 705
MspA1I CMGCKG 1 cut(s) 940
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 5 cut(s) 193, 213, 243, 658, 723
MspR9I CCNGG 7 cut(s) 194, 213, 546, 723, 724, 812, 819
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 630
MvaI CCWGG 3 cut(s) 546, 812, 819
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 3 cut(s) 98, 107, 176
NciI CCSGG 4 cut(s) 194, 213, 723, 724
NdeII GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
NlaIII CATG 3 cut(s) 271, 641, 733
NlaIV GGNNCC 3 cut(s) 169, 303, 816
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 651
NspI RCATGY 1 cut(s) 271
PauI GCGCGC 1 cut(s) 275
PceI AGGCCT 2 cut(s) 21, 155
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 369, 570
PkrI GCNGC 3 cut(s) 103, 191, 979
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 814
PshAI GACNNNNGTC 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 448
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 3 cut(s) 544, 810, 817
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 3 cut(s) 544, 810, 817
PspN4I GGNNCC 3 cut(s) 169, 303, 816
PspPI GGNCC 4 cut(s) 168, 240, 433, 814
PspPPI RGGWCCY 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 3 cut(s) 765, 919, 964
PteI GCGCGC 1 cut(s) 275
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
Rsr2I CGGWCCG 1 cut(s) 240
RsrII CGGWCCG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 448
SaqAI TTAA 2 cut(s) 462, 705
SatI GCNGC 3 cut(s) 102, 190, 978
Sau3AI GATC 8 cut(s) 174, 184, 306, 765, 778, 919, 949, 964
Sau96I GGNCC 4 cut(s) 168, 240, 433, 814
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 7 cut(s) 194, 213, 546, 723, 724, 812, 819
SduI GDGCHC 1 cut(s) 448
SetI ASST 9 cut(s) 119, 149, 348, 448, 589, 611, 679, 816, 823
SfaNI GCATC 6 cut(s) 128, 487, 679, 708, 841, 870
SfcI CTRYAG 2 cut(s) 380, 672
SinI GGWCC 2 cut(s) 240, 814
SmaI CCCGGG 1 cut(s) 724
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
SrfI GCCCGGGC 1 cut(s) 724
Sse9I AATT 2 cut(s) 522, 774
SseBI AGGCCT 2 cut(s) 21, 155
SspMI CTAG 1 cut(s) 297
SstI GAGCTC 1 cut(s) 448
StuI AGGCCT 2 cut(s) 21, 155
StyD4I CCNGG 7 cut(s) 192, 211, 544, 721, 722, 810, 817
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 313, 751
TaiI ACGT 1 cut(s) 119
TaqI TCGA 5 cut(s) 14, 201, 233, 324, 878
TasI AATT 2 cut(s) 522, 774
TauI GCSGC 2 cut(s) 104, 980
TfiI GAWTC 2 cut(s) 369, 570
Tru1I TTAA 2 cut(s) 462, 705
Tru9I TTAA 2 cut(s) 462, 705
TseFI GTSAC 1 cut(s) 651
TseI GCWGC 1 cut(s) 189
Tsp45I GTSAC 1 cut(s) 651
TspDTI ATGAA 2 cut(s) 617, 930
TspMI CCCGGG 1 cut(s) 722
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 2 cut(s) 240, 814
XceI RCATGY 1 cut(s) 271
XmaI CCCGGG 1 cut(s) 722
XspI CTAG 1 cut(s) 297
ZraI GACGTC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.