Rh4DG278100

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012310
Physical Location & Seq
Reverse (-)
49853257 .. 49855966
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG278100.1

Sequence Viewer

Length: 993 bp
ATGTCCGCCTTATCGAGAGGCCTCATTGCCCGCCTCCGCCTCCTCTCCCTCAACCCGGCCACTTCTTCTTCTTCCTCCTCTTCTACTTGTACCGCTATACCCGCCGCTCACGCTACGACGACGTCGTATTCTCATTTCTTCCGATGCTTCAGCTCCGAGGCCTTGGTGGACGGGGCCGAGATCGCCGAAGATCGCAGCCGGGTTATCGAGGCCAGGCCCGGAGTTATGACCCCCCAATCGAAGCGGACCGGAGTCATCGCCGTCAAGTGTGGCATGTCGGCGCGCTGGGACAAATGGGGTGCTAGGGTTCCGATCACCGTGCTTTGGGTCGATGATAATATCGTCTCTCAGGTCAAAACCCCAGAGAAAGAAGGAATCTCTGCCCTACAGATAGGGTGCGGGCAGAAAAAAGCAAAACATCTGACAAAGCCTGAAGTGGGCCATTTCAGAGCTCAGGGTGTTCCACTTAAGAGGAAACTGCGGGAGTTTCCGGTAACGGAAGATGCGCTTCTCCCTGTTGGTATGTCAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTTACAGGAATCACAAGAGGGAAAGGTTTTCAGGGTGGGATGAAAAGGTATGGTTTTAGAGGAATGCCAGCAAGTCATGGTGCTTCTAAGTCACACCGAAGTATTGGTTCTACAGGTCAGAGAGATGATGCTGGAAGGGTCTTTAAAGGCAGAAAGATGCCCGGGCGCATGGGTGGAAAACAGAGAACGGTGAAAAATGTATGGATCTACAAAATTGATCCCGCAAGGAACTTGATATGGGTGAGAGGCCAGGTCCCAGGTGCTGAAGGGAACTTTGTGTTTATCAAGGATGCTTTCTACAAGAAACCCAATGTCTCGATGCTACCATTTCCAACTTACTTTGCTCCAGAGGATGAAGATCCAAGCAAGTTAGAACCGCTGGTTGCTGATCTTGGAGAAGTAGATCCATTTATGGCGGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

330

Amino Acids

36.0

Weight (kDa)

9.92

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 180 - 262 9.8e-17 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 107
AccII CGCG 1 cut(s) 283
AclWI GGATC 4 cut(s) 779, 779, 920, 965
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 3 cut(s) 133, 453, 852
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 3 cut(s) 55, 324, 437
AflII CTTAAG 1 cut(s) 467
AjnI CCWGG 4 cut(s) 212, 550, 816, 823
AluBI AGCT 2 cut(s) 153, 452
AluI AGCT 2 cut(s) 153, 452
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 2 cut(s) 349, 886
AlwI GGATC 4 cut(s) 779, 779, 920, 965
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 9 cut(s) 19, 57, 159, 174, 210, 215, 439, 553, 814
ApeKI GCWGC 1 cut(s) 195
AspLEI GCGC 4 cut(s) 283, 285, 508, 735
AspS9I GGNCC 5 cut(s) 174, 216, 246, 439, 820
AsuC2I CCSGG 5 cut(s) 56, 200, 219, 729, 730
AsuHPI GGTGA 3 cut(s) 307, 769, 820
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 2 cut(s) 246, 820
BanII GRGCYC 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 1 cut(s) 207
BceAI ACGGC 1 cut(s) 245
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BciT130I CCWGG 4 cut(s) 214, 552, 818, 825
BcnI CCSGG 5 cut(s) 56, 200, 219, 729, 730
BcoDI GTCTC 2 cut(s) 349, 886
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 2 cut(s) 386, 678
BfrI CTTAAG 1 cut(s) 467
BisI GCNGC 3 cut(s) 105, 196, 984
BlsI GCNGC 3 cut(s) 106, 197, 985
Bme1390I CCNGG 9 cut(s) 56, 200, 214, 219, 552, 729, 730, 818, 825
Bme18I GGWCC 2 cut(s) 246, 820
BmeT110I CYCGRG 1 cut(s) 728
BmgT120I GGNCC 5 cut(s) 174, 216, 246, 439, 820
BmiI GGNNCC 3 cut(s) 175, 309, 822
BmrFI CCNGG 9 cut(s) 56, 200, 214, 219, 552, 729, 730, 818, 825
BmsI GCATC 6 cut(s) 134, 493, 685, 714, 847, 876
BoxI GACNNNNGTC 1 cut(s) 251
BpmI CTGGAG 1 cut(s) 897
Bpu10I CCTNAGC 1 cut(s) 453
BpuMI CCSGG 5 cut(s) 56, 200, 219, 729, 730
BsaBI GATNNNNATC 1 cut(s) 924
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 4 cut(s) 156, 162, 728, 823
BsaWI WCCGGW 2 cut(s) 248, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 324, 437
Bse3DI GCAATG 1 cut(s) 24
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 4 cut(s) 214, 552, 818, 825
BseDI CCNNGG 4 cut(s) 156, 162, 728, 823
BseGI GGATG 3 cut(s) 612, 862, 925
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 3 cut(s) 55, 324, 437
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 2 cut(s) 362, 467
BsePI GCGCGC 1 cut(s) 281
BseRI GAGGAG 2 cut(s) 32, 67
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 9 cut(s) 21, 59, 161, 176, 212, 217, 441, 555, 816
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
BslFI GGGAC 2 cut(s) 302, 806
BslI CCNNNNNNNGG 3 cut(s) 55, 324, 437
BsmAI GTCTC 2 cut(s) 349, 886
BsmBI CGTCTC 1 cut(s) 349
BsmFI GGGAC 2 cut(s) 302, 806
BsmI GAATGC 1 cut(s) 636
BsnI GGCC 9 cut(s) 21, 59, 161, 176, 212, 217, 441, 555, 816
BsoBI CYCGRG 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
BspANI GGCC 9 cut(s) 21, 59, 161, 176, 212, 217, 441, 555, 816
BspCNI CTCAG 2 cut(s) 361, 466
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 3 cut(s) 175, 309, 822
BspPI GGATC 4 cut(s) 779, 779, 920, 965
BspTI CTTAAG 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 24
BssECI CCNNGG 4 cut(s) 156, 162, 728, 823
BssHII GCGCGC 1 cut(s) 281
BssMI GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 4 cut(s) 214, 552, 818, 825
Bst4CI ACNGT 2 cut(s) 319, 757
Bst6I CTCTTC 1 cut(s) 85
BstACI GRCGYC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 467
BstC8I GCNNGC 4 cut(s) 31, 283, 401, 636
BstDEI CTNAG 3 cut(s) 348, 453, 654
BstF5I GGATG 3 cut(s) 612, 862, 925
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 4 cut(s) 283, 285, 508, 735
BstKTI GATC 8 cut(s) 183, 193, 315, 774, 787, 928, 958, 973
BstMAI GTCTC 2 cut(s) 349, 886
BstMBI GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
BstMWI GCNNNNNNNGC 3 cut(s) 101, 110, 182
BstNI CCWGG 4 cut(s) 214, 552, 818, 825
BstNSI RCATGY 1 cut(s) 277
BstPAI GACNNNNGTC 1 cut(s) 251
BstSCI CCNGG 9 cut(s) 54, 198, 212, 217, 550, 727, 728, 816, 823
BstSFI CTRYAG 2 cut(s) 386, 678
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 207
BstX2I RGATCY 3 cut(s) 771, 925, 970
BstYI RGATCY 3 cut(s) 771, 925, 970
BsuRI GGCC 9 cut(s) 21, 59, 161, 176, 212, 217, 441, 555, 816
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 3 cut(s) 612, 862, 925
Cac8I GCNNGC 4 cut(s) 31, 283, 401, 636
CaiI CAGNNNCTG 1 cut(s) 830
CfoI GCGC 4 cut(s) 283, 285, 508, 735
Cfr13I GGNCC 5 cut(s) 174, 216, 246, 439, 820
Cfr9I CCCGGG 1 cut(s) 728
CpoI CGGWCCG 1 cut(s) 246
Csp6I GTAC 1 cut(s) 90
CspI CGGWCCG 1 cut(s) 246
CviAII CATG 3 cut(s) 274, 644, 736
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 3 cut(s) 348, 453, 654
DpnI GATC 8 cut(s) 182, 192, 314, 773, 786, 927, 957, 972
DpnII GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
DraI TTTAAA 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 2 cut(s) 21, 161
Eco24I GRGCYC 1 cut(s) 454
Eco47I GGWCC 2 cut(s) 246, 820
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 3 cut(s) 133, 453, 852
Eco88I CYCGRG 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 820
EcoRII CCWGG 4 cut(s) 212, 550, 816, 823
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 349
FaeI CATG 3 cut(s) 277, 647, 739
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 2 cut(s) 302, 806
FatI CATG 3 cut(s) 273, 643, 735
FauI CCCGC 5 cut(s) 38, 109, 392, 474, 796
Fnu4HI GCNGC 3 cut(s) 105, 196, 984
FokI GGATG 3 cut(s) 619, 869, 932
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 3 cut(s) 105, 196, 984
FspBI CTAG 1 cut(s) 303
GlaI GCGC 4 cut(s) 282, 284, 507, 734
GluI GCNGC 3 cut(s) 105, 196, 984
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 897
HaeIII GGCC 9 cut(s) 21, 59, 161, 176, 212, 217, 441, 555, 816
HapII CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
HhaI GCGC 4 cut(s) 283, 285, 508, 735
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 3 cut(s) 277, 647, 739
Hin6I GCGC 4 cut(s) 281, 283, 506, 733
HinP1I GCGC 4 cut(s) 281, 283, 506, 733
HinfI GANTC 3 cut(s) 252, 375, 576
HpaII CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
HphI GGTGA 3 cut(s) 307, 769, 820
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 6 cut(s) 143, 157, 312, 423, 449, 687
Hpy188III TCNNGA 3 cut(s) 15, 883, 914
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 3 cut(s) 121, 124, 127
HpyAV CCTTC 3 cut(s) 365, 696, 827
HpyCH4III ACNGT 2 cut(s) 319, 757
HpyCH4IV ACGT 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 110, 182
HpyF3I CTNAG 3 cut(s) 348, 453, 654
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 3 cut(s) 277, 647, 739
HspAI GCGC 4 cut(s) 281, 283, 506, 733
Kzo9I GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
LmnI GCTCC 2 cut(s) 158, 916
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 6 cut(s) 134, 493, 685, 714, 847, 876
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 3 cut(s) 493, 568, 657
MalI GATC 8 cut(s) 182, 192, 314, 773, 786, 927, 957, 972
MbiI CCGCTC 1 cut(s) 107
MboI GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
MboII GAAGA 8 cut(s) 57, 60, 63, 72, 130, 200, 512, 935
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 3 cut(s) 771, 925, 970
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 2 cut(s) 528, 780
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 923
MseI TTAA 2 cut(s) 468, 711
MspA1I CMGCKG 1 cut(s) 946
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 6 cut(s) 56, 199, 219, 249, 491, 729
MspR9I CCNGG 9 cut(s) 56, 200, 214, 219, 552, 729, 730, 818, 825
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 636
MvaI CCWGG 4 cut(s) 214, 552, 818, 825
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 3 cut(s) 101, 110, 182
NciI CCSGG 5 cut(s) 56, 200, 219, 729, 730
NdeII GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
NlaIII CATG 3 cut(s) 277, 647, 739
NlaIV GGNNCC 3 cut(s) 175, 309, 822
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 657
NspI RCATGY 1 cut(s) 277
PauI GCGCGC 1 cut(s) 281
PceI AGGCCT 2 cut(s) 21, 161
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PctI GAATGC 1 cut(s) 636
PfeI GAWTC 2 cut(s) 375, 576
PflFI GACNNNGTC 1 cut(s) 121
PkrI GCNGC 3 cut(s) 106, 197, 985
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 820
PshAI GACNNNNGTC 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 820
Psp6I CCWGG 4 cut(s) 212, 550, 816, 823
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 4 cut(s) 212, 550, 816, 823
PspN4I GGNNCC 3 cut(s) 175, 309, 822
PspPI GGNCC 5 cut(s) 174, 216, 246, 439, 820
PspPPI RGGWCCY 1 cut(s) 820
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 3 cut(s) 771, 925, 970
PsyI GACNNNGTC 1 cut(s) 121
PteI GCGCGC 1 cut(s) 281
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
Rsr2I CGGWCCG 1 cut(s) 246
RsrII CGGWCCG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 468, 711
SatI GCNGC 3 cut(s) 105, 196, 984
Sau3AI GATC 8 cut(s) 180, 190, 312, 771, 784, 925, 955, 970
Sau96I GGNCC 5 cut(s) 174, 216, 246, 439, 820
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 9 cut(s) 56, 200, 214, 219, 552, 729, 730, 818, 825
SduI GDGCHC 1 cut(s) 454
SetI ASST 9 cut(s) 125, 155, 354, 454, 595, 617, 685, 822, 829
SfaNI GCATC 6 cut(s) 134, 493, 685, 714, 847, 876
SfcI CTRYAG 2 cut(s) 386, 678
SinI GGWCC 2 cut(s) 246, 820
SmaI CCCGGG 1 cut(s) 730
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SrfI GCCCGGGC 1 cut(s) 730
Sse9I AATT 2 cut(s) 528, 780
SseBI AGGCCT 2 cut(s) 21, 161
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 1 cut(s) 454
StuI AGGCCT 2 cut(s) 21, 161
StyD4I CCNGG 9 cut(s) 54, 198, 212, 217, 550, 727, 728, 816, 823
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 319, 757
TaiI ACGT 1 cut(s) 125
TaqI TCGA 5 cut(s) 14, 207, 239, 330, 884
TasI AATT 2 cut(s) 528, 780
TauI GCSGC 2 cut(s) 107, 986
TfiI GAWTC 2 cut(s) 375, 576
Tru1I TTAA 2 cut(s) 468, 711
Tru9I TTAA 2 cut(s) 468, 711
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 2 cut(s) 623, 936
TspGWI ACGGA 1 cut(s) 512
TspMI CCCGGG 1 cut(s) 728
Tth111I GACNNNGTC 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 467
VpaK11BI GGWCC 2 cut(s) 246, 820
XceI RCATGY 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 728
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.