pycom13g12130

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012310
Physical Location & Seq
Reverse (-)
8386892 .. 8389827
2936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12130.4

Sequence Viewer

Length: 984 bp
ATGTCGGCGTTATCGAGAGGCCTCATTTCCCGCCTCCGCCTTCTCTCGCTCAATCCCTCCCATTCTTCTTGCACTGCTACTGCTGCCGCTAATGCTACGACGTCGTTGACGCAGACCCAGTGCCATTTCTTCAGATGCTTCAGCTCCGAGGCCATGGTGGACGCGACGGAGGCCGTGGCTGACAGGAGACCGGTTATTGAAGCGAAGCCCGGCCCGATGAGCCCGGGTTCGAAGAGGACCGGGCTCATAGCCGTCAAGTGCGGGATGACTGCGCTCTGGGACAAATGGGGCGCTAGGGTTCCGATAACTGTGCTCTGGGTCGATGATAATATCGTCTCTCAGGTCAAGACCCCTGAGAAGGAAGGCTTCCCTGCTCTCCAGATTGGTTGCGGACAGAAGAAAGCAAAACATTTGACAAAGCCAGAGGTGGGTCATTTCCGAGGTCAGGGTGTTCCACTTAAGAGGAAATTGCACGAGTTTCCTGTATCAAATGATGCGCTTCTCCCTGTTGGTATGGAAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTCACAGGAATCACGCGAGGGAAAGGTTTCCAGGGTGGAATGAAAAGACACGGTTTTAGTGGTATGCCAGCATCCCATGGCGCATCTCTGTCACACCGAAGTATTGGTTCTACAGGTCAGAGGGATGATGCTGGAAAGGTCTTTAAAGGCAGAAAGATGCCGGGGCGCATGGGTGGGAAACAGAGAACGGTGAAAAATGTATGGGTCTACAAAATTGATCCTGCAAGGAATTTGATGTGGGTGAAAGGCCAAGTCCCAGGTGCTGAAGGGAACTTCGTTTTTATCAGAGATGCCTTCTACAAGAAACCTGATGTTTCATCGCTACCATTTCCAACCTACTTTGCTCCAGAGGATGAGGATCCATCCAAGTTAGAAACACTAGTTGCTGATCTCGGAGAAGTAGATCCGTTCATGGTGGCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

328

Amino Acids

35.55

Weight (kDa)

9.66

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 765
AccII CGCG 2 cut(s) 164, 574
AciI CCGC 5 cut(s) 31, 37, 87, 261, 390
AclWI GGATC 4 cut(s) 770, 911, 924, 956
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 115, 124, 843
AcyI GRCGYC 1 cut(s) 101
AfiI CCNNNNNNNGG 3 cut(s) 358, 428, 445
AflII CTTAAG 1 cut(s) 458
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 200
AhlI ACTAGT 1 cut(s) 937
AjnI CCWGG 3 cut(s) 541, 588, 814
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 181, 340
AlwI GGATC 4 cut(s) 770, 911, 924, 956
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 6 cut(s) 19, 150, 171, 211, 544, 805
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 787
AsiGI ACCGGT 1 cut(s) 190
Asp700I GAANNNNTTC 1 cut(s) 584
AspLEI GCGC 5 cut(s) 274, 293, 499, 641, 726
AspS9I GGNCC 2 cut(s) 212, 237
AsuC2I CCSGG 5 cut(s) 210, 224, 225, 241, 720
AsuHPI GGTGA 2 cut(s) 760, 811
AsuII TTCGAA 1 cut(s) 230
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 916
BanII GRGCYC 2 cut(s) 224, 246
BauI CACGAG 1 cut(s) 473
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 928
BceAI ACGGC 2 cut(s) 158, 236
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BciT130I CCWGG 3 cut(s) 543, 590, 816
BcnI CCSGG 5 cut(s) 210, 224, 225, 241, 720
BcoDI GTCTC 2 cut(s) 181, 340
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 2 cut(s) 294, 938
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 294
BfrI CTTAAG 1 cut(s) 458
BisI GCNGC 2 cut(s) 84, 87
BlsI GCNGC 2 cut(s) 85, 88
Bme1390I CCNGG 8 cut(s) 210, 224, 225, 241, 543, 590, 720, 816
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 212, 237
BmiI GGNNCC 2 cut(s) 300, 918
BmrFI CCNGG 8 cut(s) 210, 224, 225, 241, 543, 590, 720, 816
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 7 cut(s) 125, 484, 638, 650, 676, 705, 838
BmuI ACTGGG 1 cut(s) 112
BpmI CTGGAG 2 cut(s) 362, 888
Bpu14I TTCGAA 1 cut(s) 230
BpuMI CCSGG 5 cut(s) 210, 224, 225, 241, 720
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 9 cut(s) 147, 153, 174, 223, 439, 589, 634, 719, 814
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 428, 445
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 3 cut(s) 543, 590, 816
BseDI CCNNGG 9 cut(s) 147, 153, 174, 223, 439, 589, 634, 719, 814
BseGI GGATG 5 cut(s) 270, 629, 688, 916, 920
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 358, 428, 445
BseMII CTCAG 2 cut(s) 345, 353
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 70
Bsh1236I CGCG 2 cut(s) 164, 574
BshFI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
BshTI ACCGGT 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 5 cut(s) 191, 210, 224, 240, 719
BslFI GGGAC 2 cut(s) 293, 797
BslI CCNNNNNNNGG 3 cut(s) 358, 428, 445
BsmAI GTCTC 2 cut(s) 181, 340
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 293, 797
BsnI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 223
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 3 cut(s) 224, 246, 315
Bsp143I GATC 4 cut(s) 775, 916, 946, 961
Bsp19I CCATGG 2 cut(s) 153, 634
BspACI CCGC 5 cut(s) 31, 37, 87, 261, 390
BspANI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
BspCNI CTCAG 2 cut(s) 346, 352
BspFNI CGCG 2 cut(s) 164, 574
BspLI GGNNCC 2 cut(s) 300, 918
BspPI GGATC 4 cut(s) 770, 911, 924, 956
BspT104I TTCGAA 1 cut(s) 230
BspTI CTTAAG 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 9 cut(s) 147, 153, 174, 223, 439, 589, 634, 719, 814
BssMI GATC 4 cut(s) 775, 916, 946, 961
BssNI GRCGYC 1 cut(s) 101
BssSI CACGAG 1 cut(s) 473
BssT1I CCWWGG 2 cut(s) 153, 634
Bst2BI CACGAG 1 cut(s) 473
Bst2UI CCWGG 3 cut(s) 543, 590, 816
Bst4CI ACNGT 3 cut(s) 310, 611, 748
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 458
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 2 cut(s) 339, 354
BstDSI CCRYGG 3 cut(s) 153, 174, 634
BstF5I GGATG 5 cut(s) 270, 629, 688, 916, 920
BstFNI CGCG 2 cut(s) 164, 574
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 5 cut(s) 274, 293, 499, 641, 726
BstKTI GATC 4 cut(s) 778, 919, 949, 964
BstMAI GTCTC 2 cut(s) 181, 340
BstMBI GATC 4 cut(s) 775, 916, 946, 961
BstMWI GCNNNNNNNGC 4 cut(s) 83, 92, 170, 219
BstNI CCWGG 3 cut(s) 543, 590, 816
BstSCI CCNGG 8 cut(s) 208, 222, 223, 239, 541, 588, 718, 814
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 2 cut(s) 164, 574
BstV1I GCAGC 1 cut(s) 70
BstX2I RGATCY 2 cut(s) 916, 961
BstYI RGATCY 2 cut(s) 916, 961
BsuRI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
BtgI CCRYGG 3 cut(s) 153, 174, 634
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 5 cut(s) 270, 629, 688, 916, 920
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 125
Cac8I GCNNGC 1 cut(s) 627
CaiI CAGNNNCTG 1 cut(s) 821
CfoI GCGC 5 cut(s) 274, 293, 499, 641, 726
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 212, 237
Cfr9I CCCGGG 1 cut(s) 223
CseI GACGC 2 cut(s) 118, 170
CspAI ACCGGT 1 cut(s) 190
CviAII CATG 4 cut(s) 154, 635, 727, 970
DdeI CTNAG 2 cut(s) 339, 354
DpnI GATC 4 cut(s) 777, 918, 948, 963
DpnII GATC 4 cut(s) 775, 916, 946, 961
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 153, 634
Eco147I AGGCCT 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 224, 246
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 237
Eco57I CTGAAG 3 cut(s) 115, 124, 843
Eco88I CYCGRG 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 541, 588, 814
EcoT14I CCWWGG 2 cut(s) 153, 634
EcoT38I GRGCYC 2 cut(s) 224, 246
ErhI CCWWGG 2 cut(s) 153, 634
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 4 cut(s) 157, 638, 730, 973
FaiI YATR 9 cut(s) 155, 248, 515, 552, 623, 636, 728, 760, 971
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 2 cut(s) 293, 797
FatI CATG 4 cut(s) 153, 634, 726, 969
FauI CCCGC 2 cut(s) 38, 254
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 2 cut(s) 84, 87
FokI GGATG 5 cut(s) 277, 616, 695, 907, 923
FriOI GRGCYC 2 cut(s) 224, 246
Fsp4HI GCNGC 2 cut(s) 84, 87
FspBI CTAG 2 cut(s) 294, 938
GlaI GCGC 5 cut(s) 273, 292, 498, 640, 725
GluI GCNGC 2 cut(s) 84, 87
GsuI CTGGAG 2 cut(s) 362, 888
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
HapII CCGG 5 cut(s) 191, 210, 224, 240, 719
HgaI GACGC 2 cut(s) 118, 170
HhaI GCGC 5 cut(s) 274, 293, 499, 641, 726
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 4 cut(s) 157, 638, 730, 973
Hin6I GCGC 5 cut(s) 272, 291, 497, 639, 724
HinP1I GCGC 5 cut(s) 272, 291, 497, 639, 724
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HinfI GANTC 1 cut(s) 567
HpaII CCGG 5 cut(s) 191, 210, 224, 240, 719
HphI GGTGA 2 cut(s) 760, 811
Hpy166II GTNNAC 3 cut(s) 108, 160, 766
Hpy188I TCNGA 7 cut(s) 134, 148, 303, 440, 678, 845, 953
Hpy188III TCNNGA 4 cut(s) 15, 346, 379, 905
Hpy8I GTNNAC 3 cut(s) 108, 160, 766
Hpy99I CGWCG 3 cut(s) 103, 106, 169
HpyAV CCTTC 5 cut(s) 50, 352, 356, 818, 862
HpyCH4III ACNGT 3 cut(s) 310, 611, 748
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 72, 472, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 92, 170, 219
HpyF3I CTNAG 2 cut(s) 339, 354
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 4 cut(s) 157, 638, 730, 973
HspAI GCGC 5 cut(s) 272, 291, 497, 639, 724
Kzo9I GATC 4 cut(s) 775, 916, 946, 961
LmnI GCTCC 2 cut(s) 149, 907
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 7 cut(s) 125, 484, 638, 650, 676, 705, 838
MaeI CTAG 2 cut(s) 294, 938
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 559, 648
MalI GATC 4 cut(s) 777, 918, 948, 963
MboI GATC 4 cut(s) 775, 916, 946, 961
MboII GAAGA 4 cut(s) 57, 121, 244, 409
MflI RGATCY 2 cut(s) 916, 961
MhlI GDGCHC 3 cut(s) 224, 246, 315
MluCI AATT 4 cut(s) 467, 519, 771, 787
MmeI TCCRAC 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 584
MseI TTAA 3 cut(s) 459, 702, 982
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 5 cut(s) 191, 210, 224, 240, 719
MspR9I CCNGG 8 cut(s) 210, 224, 225, 241, 543, 590, 720, 816
MvaI CCWGG 3 cut(s) 543, 590, 816
MvnI CGCG 2 cut(s) 164, 574
MwoI GCNNNNNNNGC 4 cut(s) 83, 92, 170, 219
NciI CCSGG 5 cut(s) 210, 224, 225, 241, 720
NcoI CCATGG 2 cut(s) 153, 634
NdeII GATC 4 cut(s) 775, 916, 946, 961
NlaIII CATG 4 cut(s) 157, 638, 730, 973
NlaIV GGNNCC 2 cut(s) 300, 918
NmuCI GTSAC 2 cut(s) 559, 648
NspV TTCGAA 1 cut(s) 230
PceI AGGCCT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 1 cut(s) 567
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 2 cut(s) 85, 88
Psp6I CCWGG 3 cut(s) 541, 588, 814
PspGI CCWGG 3 cut(s) 541, 588, 814
PspN4I GGNNCC 2 cut(s) 300, 918
PspPI GGNCC 2 cut(s) 212, 237
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 2 cut(s) 916, 961
SaqAI TTAA 3 cut(s) 459, 702, 982
SatI GCNGC 2 cut(s) 84, 87
Sau3AI GATC 4 cut(s) 775, 916, 946, 961
Sau96I GGNCC 2 cut(s) 212, 237
ScrFI CCNGG 8 cut(s) 210, 224, 225, 241, 543, 590, 720, 816
SduI GDGCHC 3 cut(s) 224, 246, 315
SfaNI GCATC 7 cut(s) 125, 484, 638, 650, 676, 705, 838
SfcI CTRYAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 230
SinI GGWCC 1 cut(s) 237
SmaI CCCGGG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SpeI ACTAGT 1 cut(s) 937
Sse9I AATT 4 cut(s) 467, 519, 771, 787
SseBI AGGCCT 1 cut(s) 21
SsiI CCGC 5 cut(s) 31, 37, 87, 261, 390
SspMI CTAG 2 cut(s) 294, 938
StuI AGGCCT 1 cut(s) 21
StyD4I CCNGG 8 cut(s) 208, 222, 223, 239, 541, 588, 718, 814
StyI CCWWGG 2 cut(s) 153, 634
TaaI ACNGT 3 cut(s) 310, 611, 748
TaiI ACGT 1 cut(s) 104
TaqI TCGA 3 cut(s) 14, 230, 321
TasI AATT 4 cut(s) 467, 519, 771, 787
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 3 cut(s) 459, 702, 982
Tru9I TTAA 3 cut(s) 459, 702, 982
TscAI CASTG 2 cut(s) 79, 125
TseFI GTSAC 2 cut(s) 559, 648
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 2 cut(s) 559, 648
TspDTI ATGAA 3 cut(s) 614, 864, 958
TspGWI ACGGA 2 cut(s) 182, 954
TspMI CCCGGG 1 cut(s) 223
TspRI CASTG 2 cut(s) 79, 125
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 237
XapI RAATTY 1 cut(s) 787
XmaI CCCGGG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 2 cut(s) 294, 938
ZraI GACGTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.