MD13G1139500.v1.1

50S ribosomal protein L3-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012310
Physical Location & Seq
Reverse (-)
10750863 .. 10754180
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1139500.v1.1.491

Sequence Viewer

Length: 984 bp
ATGTCGGCATTATCGAGAGGCCTCATTTCCCGCCTCCGCCTTCTCTCGCTCAACCCCTCCCCTTCTTCTTGCACTGCTACTCCTGCCGCTAATGCTATGACGTCGTTGACGCAGACCCAGTGCCATTTCTTCAGATGCTTCAGCTCCGAGGCCATGGTGGACGCGACGGAGGCCGTGGCGGACAGGAGACCGGTTATTGAAGCGAAGCCCGGCCCCATGAGCCCGGGTTCGAAGAGGACCGGGCTCATAGCCGTCAAGTGCGGGATGACTGCGCTCTGGGACAAATGGGGCGCTAGGGTTCCGATAACTGTGCTCTGGGTCGATGATAATATCGTCTCTCAGGTCAAGACCCCTGAGAAGGAAGGCTTCCCTGCTCTCCAGATTGGTTGCGGACAGAAGAAAGCAAAACATTTGACAAAGCCAGAGGTGGGTCATTTCCGAGGTCAGGGTGTTCCACTTAAGAGGAAATTGCATGAGTTTCCTGTATCAAATGATGCGCTCCTCCCTGTTGGTATGGGAATTGGTGTTCGCCATTTTGTTCCAGGCCAATATGTTGATGTCACAGGAATCACGCGAGGGAAAGGTTTCCAGGGTGGAATGAAAAGACACGGTTTTAGTGGTATGCCAGCATCCCATGGCGCATCTCTGTCGCACCGAAGTATTGGTTCTACAGGTCAGAGGGATGATGCTGGAAAGGTTTTTAAAGGCAGAAAGATGCCGGGGCGCATGGGTGGGAAACAGAGAACGGTGAAAAATGTATGGGTCTACAAAATCGATCCTGCAAGGAATTTGATGTGGGTGAAAGGCCAAGTCCCGGGTGCTGAAGGGAACTTCGTTTTCATCAGAGATGCTTTCTACAAGAAACCTGATGTTTCATCGCTACCATTTCCAACCTACTTTGCTCCAGAGGATGAGGATCCATCCAAGTTAGAACCGCTAGTTGCTGATCTCGGAGAAGTAGATCCGTTCATGGTGGCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L3

Protein Analysis

328

Amino Acids

35.49

Weight (kDa)

9.72

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 177 - 260 2.1e-15 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012310)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17465
fragaria_vesca FvH4_4g21510
malus_domestica MD13G1139500.v1.1 MD16G1136200.v1.1
prunus_persica Prupe.1G105200_v2.0.a1
pyrus_communis pycom13g12130 pycom16g11980
rosa_chinensis RchiOBHm_Chr4g0427531
rosa_laevigata RLG00000007230
rosa_multiflora Rmu_sc0009627.1_g000003 Rmu_sc0020720.1_g000001
rosa_roxburghii Rroxscaffold_5G00369170
rosa_rugosa Rorug04G0218400 Rorug04G0218400
rosa_samantha Rh4AG274900 Rh4BG280700 Rh4CG296000 Rh4DG278100
rosa_wichuraiana Rw4G023830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 765
AccII CGCG 2 cut(s) 164, 574
AciI CCGC 7 cut(s) 31, 37, 87, 179, 261, 390, 935
AclWI GGATC 4 cut(s) 770, 911, 924, 956
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 115, 124, 843
AcyI GRCGYC 1 cut(s) 101
AfiI CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
AflII CTTAAG 1 cut(s) 458
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 200
AjnI CCWGG 2 cut(s) 541, 588
AloI GAACNNNNNNTCC 2 cut(s) 510, 542
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 181, 340
AlwI GGATC 4 cut(s) 770, 911, 924, 956
Ama87I CYCGRG 2 cut(s) 223, 814
AoxI GGCC 6 cut(s) 19, 150, 171, 211, 544, 805
ApoI RAATTY 1 cut(s) 787
AsiGI ACCGGT 1 cut(s) 190
Asp700I GAANNNNTTC 1 cut(s) 584
AspLEI GCGC 5 cut(s) 274, 293, 499, 641, 726
AspS9I GGNCC 2 cut(s) 212, 237
AsuC2I CCSGG 7 cut(s) 210, 224, 225, 241, 720, 815, 816
AsuHPI GGTGA 2 cut(s) 760, 811
AsuII TTCGAA 1 cut(s) 230
AvaI CYCGRG 2 cut(s) 223, 814
AvaII GGWCC 1 cut(s) 237
BamHI GGATCC 1 cut(s) 916
BanII GRGCYC 2 cut(s) 224, 246
Bbv12I GWGCWC 1 cut(s) 315
BccI CCATC 1 cut(s) 928
BceAI ACGGC 2 cut(s) 158, 236
BcgI CGANNNNNNTGC 4 cut(s) 292, 326, 630, 664
BciT130I CCWGG 2 cut(s) 543, 590
BcnI CCSGG 7 cut(s) 210, 224, 225, 241, 720, 815, 816
BcoDI GTCTC 2 cut(s) 181, 340
BfaI CTAG 2 cut(s) 294, 938
BfmI CTRYAG 1 cut(s) 669
BfoI RGCGCY 1 cut(s) 294
BfrI CTTAAG 1 cut(s) 458
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 2 cut(s) 223, 814
BmgT120I GGNCC 2 cut(s) 212, 237
BmiI GGNNCC 3 cut(s) 214, 300, 918
BmrFI CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 7 cut(s) 125, 484, 638, 650, 676, 705, 838
BmuI ACTGGG 1 cut(s) 112
BpmI CTGGAG 2 cut(s) 362, 888
Bpu14I TTCGAA 1 cut(s) 230
BpuMI CCSGG 7 cut(s) 210, 224, 225, 241, 720, 815, 816
Bsa29I ATCGAT 1 cut(s) 774
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 9 cut(s) 147, 153, 174, 223, 439, 589, 634, 719, 814
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
Bse118I RCCGGY 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 2 cut(s) 543, 590
BseCI ATCGAT 1 cut(s) 774
BseDI CCNNGG 9 cut(s) 147, 153, 174, 223, 439, 589, 634, 719, 814
BseGI GGATG 5 cut(s) 270, 629, 688, 916, 920
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
BseMII CTCAG 2 cut(s) 345, 353
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 491
Bsh1236I CGCG 2 cut(s) 164, 574
BshFI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
BshTI ACCGGT 1 cut(s) 190
BshVI ATCGAT 1 cut(s) 774
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 2 cut(s) 223, 814
BsiSI CCGG 6 cut(s) 191, 210, 224, 240, 719, 815
BslFI GGGAC 2 cut(s) 293, 797
BslI CCNNNNNNNGG 4 cut(s) 358, 428, 445, 814
BsmAI GTCTC 2 cut(s) 181, 340
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 293, 797
BsnI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 2 cut(s) 223, 814
Bsp119I TTCGAA 1 cut(s) 230
Bsp1286I GDGCHC 3 cut(s) 224, 246, 315
Bsp143I GATC 4 cut(s) 775, 916, 946, 961
Bsp19I CCATGG 2 cut(s) 153, 634
BspACI CCGC 7 cut(s) 31, 37, 87, 179, 261, 390, 935
BspANI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
BspCNI CTCAG 2 cut(s) 346, 352
BspDI ATCGAT 1 cut(s) 774
BspFNI CGCG 2 cut(s) 164, 574
BspLI GGNNCC 3 cut(s) 214, 300, 918
BspPI GGATC 4 cut(s) 770, 911, 924, 956
BspT104I TTCGAA 1 cut(s) 230
BspTI CTTAAG 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 190
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 9 cut(s) 147, 153, 174, 223, 439, 589, 634, 719, 814
BssMI GATC 4 cut(s) 775, 916, 946, 961
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 2 cut(s) 153, 634
Bst2UI CCWGG 2 cut(s) 543, 590
Bst4CI ACNGT 3 cut(s) 310, 611, 748
Bst6I CTCTTC 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 458
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 2 cut(s) 339, 354
BstDSI CCRYGG 3 cut(s) 153, 174, 634
BstF5I GGATG 5 cut(s) 270, 629, 688, 916, 920
BstFNI CGCG 2 cut(s) 164, 574
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 5 cut(s) 274, 293, 499, 641, 726
BstKTI GATC 4 cut(s) 778, 919, 949, 964
BstMAI GTCTC 2 cut(s) 181, 340
BstMBI GATC 4 cut(s) 775, 916, 946, 961
BstMWI GCNNNNNNNGC 4 cut(s) 83, 92, 170, 219
BstNI CCWGG 2 cut(s) 543, 590
BstSCI CCNGG 9 cut(s) 208, 222, 223, 239, 541, 588, 718, 813, 814
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 2 cut(s) 164, 574
BstX2I RGATCY 2 cut(s) 916, 961
BstYI RGATCY 2 cut(s) 916, 961
Bsu15I ATCGAT 1 cut(s) 774
BsuRI GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
BsuTUI ATCGAT 1 cut(s) 774
BtgI CCRYGG 3 cut(s) 153, 174, 634
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 5 cut(s) 270, 629, 688, 916, 920
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 125
Cac8I GCNNGC 1 cut(s) 627
CfoI GCGC 5 cut(s) 274, 293, 499, 641, 726
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 212, 237
Cfr9I CCCGGG 2 cut(s) 223, 814
ClaI ATCGAT 1 cut(s) 774
CseI GACGC 2 cut(s) 118, 170
CspAI ACCGGT 1 cut(s) 190
CviAII CATG 6 cut(s) 154, 217, 473, 635, 727, 970
DdeI CTNAG 2 cut(s) 339, 354
DpnI GATC 4 cut(s) 777, 918, 948, 963
DpnII GATC 4 cut(s) 775, 916, 946, 961
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
EciI GGCGGA 2 cut(s) 26, 194
Eco130I CCWWGG 2 cut(s) 153, 634
Eco147I AGGCCT 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 224, 246
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 237
Eco57I CTGAAG 3 cut(s) 115, 124, 843
Eco88I CYCGRG 2 cut(s) 223, 814
EcoRII CCWGG 2 cut(s) 541, 588
EcoT14I CCWWGG 2 cut(s) 153, 634
EcoT38I GRGCYC 2 cut(s) 224, 246
ErhI CCWWGG 2 cut(s) 153, 634
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 6 cut(s) 157, 220, 476, 638, 730, 973
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 2 cut(s) 293, 797
FatI CATG 6 cut(s) 153, 216, 472, 634, 726, 969
FauI CCCGC 2 cut(s) 38, 254
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 5 cut(s) 277, 616, 695, 907, 923
FriOI GRGCYC 2 cut(s) 224, 246
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 2 cut(s) 294, 938
GlaI GCGC 5 cut(s) 273, 292, 498, 640, 725
GluI GCNGC 1 cut(s) 87
GsuI CTGGAG 2 cut(s) 362, 888
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 6 cut(s) 21, 152, 173, 213, 546, 807
HapII CCGG 6 cut(s) 191, 210, 224, 240, 719, 815
HgaI GACGC 2 cut(s) 118, 170
HhaI GCGC 5 cut(s) 274, 293, 499, 641, 726
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 6 cut(s) 157, 220, 476, 638, 730, 973
Hin6I GCGC 5 cut(s) 272, 291, 497, 639, 724
HinP1I GCGC 5 cut(s) 272, 291, 497, 639, 724
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HinfI GANTC 1 cut(s) 567
HpaII CCGG 6 cut(s) 191, 210, 224, 240, 719, 815
HphI GGTGA 2 cut(s) 760, 811
Hpy166II GTNNAC 3 cut(s) 108, 160, 766
Hpy188I TCNGA 7 cut(s) 134, 148, 303, 440, 678, 845, 953
Hpy188III TCNNGA 4 cut(s) 15, 346, 379, 905
Hpy8I GTNNAC 3 cut(s) 108, 160, 766
Hpy99I CGWCG 2 cut(s) 106, 169
HpyAV CCTTC 5 cut(s) 50, 72, 352, 356, 818
HpyCH4III ACNGT 3 cut(s) 310, 611, 748
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 72, 472, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 92, 170, 219
HpyF3I CTNAG 2 cut(s) 339, 354
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 6 cut(s) 157, 220, 476, 638, 730, 973
HspAI GCGC 5 cut(s) 272, 291, 497, 639, 724
Kzo9I GATC 4 cut(s) 775, 916, 946, 961
LmnI GCTCC 3 cut(s) 149, 504, 907
LweI GCATC 7 cut(s) 125, 484, 638, 650, 676, 705, 838
MaeI CTAG 2 cut(s) 294, 938
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 1 cut(s) 559
MalI GATC 4 cut(s) 777, 918, 948, 963
MboI GATC 4 cut(s) 775, 916, 946, 961
MboII GAAGA 4 cut(s) 57, 121, 244, 409
MflI RGATCY 2 cut(s) 916, 961
MhlI GDGCHC 3 cut(s) 224, 246, 315
MluCI AATT 3 cut(s) 467, 519, 787
MmeI TCCRAC 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 584
MseI TTAA 3 cut(s) 459, 702, 982
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 6 cut(s) 191, 210, 224, 240, 719, 815
MspR9I CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
MvaI CCWGG 2 cut(s) 543, 590
MvnI CGCG 2 cut(s) 164, 574
MwoI GCNNNNNNNGC 4 cut(s) 83, 92, 170, 219
NciI CCSGG 7 cut(s) 210, 224, 225, 241, 720, 815, 816
NcoI CCATGG 2 cut(s) 153, 634
NdeII GATC 4 cut(s) 775, 916, 946, 961
NlaIII CATG 6 cut(s) 157, 220, 476, 638, 730, 973
NlaIV GGNNCC 3 cut(s) 214, 300, 918
NmuCI GTSAC 1 cut(s) 559
NspV TTCGAA 1 cut(s) 230
PceI AGGCCT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 1 cut(s) 567
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 541, 588
PspGI CCWGG 2 cut(s) 541, 588
PspN4I GGNNCC 3 cut(s) 214, 300, 918
PspPI GGNCC 2 cut(s) 212, 237
PsuI RGATCY 2 cut(s) 916, 961
SaqAI TTAA 3 cut(s) 459, 702, 982
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 4 cut(s) 775, 916, 946, 961
Sau96I GGNCC 2 cut(s) 212, 237
ScrFI CCNGG 9 cut(s) 210, 224, 225, 241, 543, 590, 720, 815, 816
SduI GDGCHC 3 cut(s) 224, 246, 315
SfaNI GCATC 7 cut(s) 125, 484, 638, 650, 676, 705, 838
SfcI CTRYAG 1 cut(s) 669
SfuI TTCGAA 1 cut(s) 230
SinI GGWCC 1 cut(s) 237
SmaI CCCGGG 2 cut(s) 225, 816
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 3 cut(s) 467, 519, 787
SseBI AGGCCT 1 cut(s) 21
SsiI CCGC 7 cut(s) 31, 37, 87, 179, 261, 390, 935
SspMI CTAG 2 cut(s) 294, 938
StuI AGGCCT 1 cut(s) 21
StyD4I CCNGG 9 cut(s) 208, 222, 223, 239, 541, 588, 718, 813, 814
StyI CCWWGG 2 cut(s) 153, 634
TaaI ACNGT 3 cut(s) 310, 611, 748
TaiI ACGT 1 cut(s) 104
TaqI TCGA 4 cut(s) 14, 230, 321, 774
TasI AATT 3 cut(s) 467, 519, 787
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 3 cut(s) 459, 702, 982
Tru9I TTAA 3 cut(s) 459, 702, 982
TscAI CASTG 2 cut(s) 79, 125
TseFI GTSAC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 4 cut(s) 614, 829, 864, 958
TspGWI ACGGA 2 cut(s) 182, 954
TspMI CCCGGG 2 cut(s) 223, 814
TspRI CASTG 2 cut(s) 79, 125
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 237
XapI RAATTY 1 cut(s) 787
XmaI CCCGGG 2 cut(s) 223, 814
XmiI GTMKAC 1 cut(s) 765
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 2 cut(s) 294, 938
ZraI GACGTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.