FvH4_4g23440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013315
Physical Location & Seq
Forward (+)
25914993 .. 25916451
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23440.t1

Sequence Viewer

Length: 786 bp
ATGAAGATAAAGAACAAAGGCAAAGTCCACCCATCTCCTTCCTCCTCCTCCTCCTCTTCTTCCGATGGAGTAGTTCTCTCCGTCCTCCAGCTACTCCCAGCTGCTATTCTAGCTCTGGCCTCGGTTCTCTCCCTCGAAGACCGCGAGGTCCTGGCTTACATGATAACCTGCTCCATGAAAACCGCACCCTCTACTTCCTCTTCTTCTTCCTCGAACCCTCAAGATTTCAAGAAGAAGTCTTCGAAGAAATTAACGAGTAAGAATAACAAGAACCACCAGTTCGATTGCGAGTGCTTCGACTGCTACCGAAGCTACTGGTTCAGGTGGGACTCCTCCCCCAACCGCGAGCTCATCCACCAGGCTATTGAGGCCTTCGAGGACCATCTGGCCCACGGCGAGAAGCCCAAGAAGAACGCCGGGAGGGGGAAGCGGAGAGGAGACAAGACGGGCCGCCGCGAGGCTCAGAAATCAGCCGAGGGTTCCTCTAAAGATGAAATCTTTGTTGTGGCGGCGCCGGCGGCGGAGGAGGAGAGTGTCAATGTGGCAGTGAGCTTGGTTCCCGAATTGGTGCTGCCGGTTTGCGACGTAGTTTCGGAGAGAGTTGAGGAGGAGTTAGGAGTGGAAGAGGAGGAAGCTGTGGAGGTTAAGGTGGAGGAGGACGACGTGGCGTTGATTGTACGCGCCGCGGCGGTGAGCAACCACAAGGGTCTGGCAAGGAAAGTACTTCCGGACGTGTTGGGGCTATTCAATTCTCGTTTGTGGAATCTTTGGAGTCCGAATGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

28.62

Weight (kDa)

6.13

Isoelectric Point (pI)

67.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13090
fragaria_vesca FvH4_4g23440
malus_domestica MD05G1113800.v1.1 MD13G1122500.v1.1 MD16G1123000.v1.1
prunus_persica Prupe.1G222700_v2.0.a1 Prupe.6G184300_v2.0.a1
pyrus_communis pycom13g10660
rosa_chinensis RchiOBHm_Chr4g0429791
rosa_laevigata RLG00000007042
rosa_multiflora Rmu_co8022056.1_g000001 Rmu_sc0018457.1_g000001
rosa_roxburghii Rroxscaffold_5G00371570
rosa_rugosa Rorug04G0238400
rosa_samantha Rh4BG300700 Rh4CG316200 Rh4DG297800
rosa_wichuraiana Rw4G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 511
AccII CGCG 5 cut(s) 144, 345, 456, 681, 686
AccIII TCCGGA 1 cut(s) 727
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 2 cut(s) 678, 723
AfiI CCNNNNNNNGG 2 cut(s) 423, 457
AflIII ACRYGT 1 cut(s) 732
AgsI TTSAA 2 cut(s) 229, 748
AjiI CACGTC 2 cut(s) 664, 733
AjnI CCWGG 2 cut(s) 150, 357
AluBI AGCT 7 cut(s) 91, 101, 113, 312, 349, 552, 635
AluI AGCT 7 cut(s) 91, 101, 113, 312, 349, 552, 635
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 1 cut(s) 432
Aor13HI TCCGGA 1 cut(s) 727
AoxI GGCC 4 cut(s) 117, 369, 387, 448
ApeKI GCWGC 2 cut(s) 101, 571
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AspLEI GCGC 2 cut(s) 514, 683
AspS9I GGNCC 4 cut(s) 148, 379, 388, 448
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 703
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 2 cut(s) 148, 379
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 1 cut(s) 351
BbsI GAAGAC 2 cut(s) 144, 231
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 88, 558
BccI CCATC 3 cut(s) 40, 59, 390
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 2 cut(s) 152, 359
BcnI CCSGG 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 8 cut(s) 102, 451, 454, 510, 519, 572, 684, 687
BlsI GCNGC 8 cut(s) 103, 452, 455, 511, 520, 573, 685, 688
BmcAI AGTACT 1 cut(s) 723
Bme1390I CCNGG 3 cut(s) 152, 359, 418
Bme18I GGWCC 2 cut(s) 148, 379
BmgBI CACGTC 2 cut(s) 664, 733
BmgT120I GGNCC 4 cut(s) 148, 379, 388, 448
BmiI GGNNCC 3 cut(s) 481, 513, 558
BmrFI CCNGG 3 cut(s) 152, 359, 418
BpiI GAAGAC 2 cut(s) 144, 231
BplI GAGNNNNNCTC 4 cut(s) 60, 92, 467, 499
BpmI CTGGAG 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 418
BsaHI GRCGYC 1 cut(s) 512
BsaJI CCNNGG 4 cut(s) 120, 391, 474, 684
BsaWI WCCGGW 1 cut(s) 727
Bsc4I CCNNNNNNNGG 2 cut(s) 423, 457
Bse118I RCCGGY 2 cut(s) 514, 574
Bse1I ACTGG 2 cut(s) 277, 320
BseAI TCCGGA 1 cut(s) 727
BseBI CCWGG 2 cut(s) 152, 359
BseDI CCNNGG 4 cut(s) 120, 391, 474, 684
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 423, 457
BseMII CTCAG 1 cut(s) 476
BseNI ACTGG 2 cut(s) 277, 320
BseXI GCAGC 2 cut(s) 88, 558
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 5 cut(s) 144, 345, 456, 681, 686
BshFI GGCC 4 cut(s) 119, 371, 389, 450
BshNI GGYRCC 1 cut(s) 511
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 4 cut(s) 417, 515, 575, 728
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 2 cut(s) 423, 457
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 341
BsmI GAATGC 1 cut(s) 784
BsnI GGCC 4 cut(s) 119, 371, 389, 450
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 351
Bsp13I TCCGGA 1 cut(s) 727
BspANI GGCC 4 cut(s) 119, 371, 389, 450
BspCNI CTCAG 1 cut(s) 475
BspEI TCCGGA 1 cut(s) 727
BspFNI CGCG 5 cut(s) 144, 345, 456, 681, 686
BspLI GGNNCC 3 cut(s) 481, 513, 558
BspMI ACCTGC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 511
BsrFI RCCGGY 2 cut(s) 514, 574
BsrI ACTGG 2 cut(s) 277, 320
BssAI RCCGGY 2 cut(s) 514, 574
BssECI CCNNGG 4 cut(s) 120, 391, 474, 684
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 2 cut(s) 152, 359
Bst6I CTCTTC 3 cut(s) 61, 205, 618
BstACI GRCGYC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 347, 516
BstDEI CTNAG 1 cut(s) 462
BstDSI CCRYGG 2 cut(s) 391, 684
BstF5I GGATG 1 cut(s) 351
BstFNI CGCG 5 cut(s) 144, 345, 456, 681, 686
BstH2I RGCGCY 1 cut(s) 515
BstHHI GCGC 2 cut(s) 514, 683
BstMAI GTCTC 1 cut(s) 432
BstMWI GCNNNNNNNGC 6 cut(s) 110, 300, 309, 368, 515, 518
BstNI CCWGG 2 cut(s) 152, 359
BstSCI CCNGG 3 cut(s) 150, 357, 416
BstUI CGCG 5 cut(s) 144, 345, 456, 681, 686
BstV1I GCAGC 2 cut(s) 88, 558
BstV2I GAAGAC 2 cut(s) 144, 231
BsuRI GGCC 4 cut(s) 119, 371, 389, 450
BtgI CCRYGG 2 cut(s) 391, 684
BtrI CACGTC 2 cut(s) 664, 733
BtsCI GGATG 1 cut(s) 351
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 1 cut(s) 552
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 2 cut(s) 347, 516
CfoI GCGC 2 cut(s) 514, 683
Cfr10I RCCGGY 2 cut(s) 514, 574
Cfr13I GGNCC 4 cut(s) 148, 379, 388, 448
Cfr42I CCGCGG 1 cut(s) 687
Csp6I GTAC 2 cut(s) 677, 722
CviAII CATG 2 cut(s) 160, 175
CviQI GTAC 2 cut(s) 677, 722
DdeI CTNAG 1 cut(s) 462
DinI GGCGCC 1 cut(s) 513
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
Eam1104I CTCTTC 3 cut(s) 61, 205, 618
EarI CTCTTC 3 cut(s) 61, 205, 618
EciI GGCGGA 1 cut(s) 536
Ecl136II GAGCTC 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 371
Eco24I GRGCYC 1 cut(s) 351
Eco47I GGWCC 2 cut(s) 148, 379
Eco53kI GAGCTC 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 349
EcoO109I RGGNCCY 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 150, 357
EcoT38I GRGCYC 1 cut(s) 351
EgeI GGCGCC 1 cut(s) 513
EheI GGCGCC 1 cut(s) 513
FaeI CATG 2 cut(s) 163, 178
FaiI YATR 2 cut(s) 161, 176
FaqI GGGAC 1 cut(s) 341
FatI CATG 2 cut(s) 159, 174
Fnu4HI GCNGC 8 cut(s) 102, 451, 454, 510, 519, 572, 684, 687
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 8 cut(s) 102, 451, 454, 510, 519, 572, 684, 687
FspBI CTAG 1 cut(s) 110
GlaI GCGC 2 cut(s) 513, 682
GluI GCNGC 8 cut(s) 102, 451, 454, 510, 519, 572, 684, 687
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 515
HaeIII GGCC 4 cut(s) 119, 371, 389, 450
HapII CCGG 4 cut(s) 417, 515, 575, 728
HhaI GCGC 2 cut(s) 514, 683
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 2 cut(s) 163, 178
Hin6I GCGC 2 cut(s) 512, 681
HinP1I GCGC 2 cut(s) 512, 681
HinfI GANTC 3 cut(s) 329, 763, 772
HpaII CCGG 4 cut(s) 417, 515, 575, 728
HphI GGTGA 1 cut(s) 703
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 64, 465, 595, 777
Hpy188III TCNNGA 4 cut(s) 221, 229, 560, 728
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 2 cut(s) 587, 665
HpyAV CCTTC 2 cut(s) 48, 382
HpyCH4IV ACGT 3 cut(s) 585, 663, 732
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 300, 309, 368, 515, 518
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 3 cut(s) 585, 663, 732
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 2 cut(s) 163, 178
HspAI GCGC 2 cut(s) 512, 681
KasI GGCGCC 1 cut(s) 511
Kpn2I TCCGGA 1 cut(s) 727
KroI GCCGGC 1 cut(s) 514
KroNI GCCGGC 1 cut(s) 516
KspI CCGCGG 1 cut(s) 687
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 2 cut(s) 88, 558
MaeI CTAG 1 cut(s) 110
MaeII ACGT 3 cut(s) 585, 663, 732
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 3 cut(s) 248, 563, 748
Mly113I GGCGCC 1 cut(s) 512
MlyI GAGTC 2 cut(s) 323, 781
MreI CGCCGGCG 1 cut(s) 514
MroI TCCGGA 1 cut(s) 727
MroNI GCCGGC 1 cut(s) 514
MseI TTAA 2 cut(s) 251, 645
MspA1I CMGCKG 2 cut(s) 101, 686
MspI CCGG 4 cut(s) 417, 515, 575, 728
MspR9I CCNGG 3 cut(s) 152, 359, 418
Mva1269I GAATGC 1 cut(s) 784
MvaI CCWGG 2 cut(s) 152, 359
MvnI CGCG 5 cut(s) 144, 345, 456, 681, 686
MwoI GCNNNNNNNGC 6 cut(s) 110, 300, 309, 368, 515, 518
NaeI GCCGGC 1 cut(s) 516
NarI GGCGCC 1 cut(s) 512
NciI CCSGG 1 cut(s) 418
NgoMIV GCCGGC 1 cut(s) 514
NlaIII CATG 2 cut(s) 163, 178
NlaIV GGNNCC 3 cut(s) 481, 513, 558
NmeAIII GCCGAG 1 cut(s) 499
NspV TTCGAA 1 cut(s) 242
PceI AGGCCT 1 cut(s) 371
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 784
PdiI GCCGGC 1 cut(s) 516
PfeI GAWTC 1 cut(s) 763
PkrI GCNGC 8 cut(s) 103, 452, 455, 511, 520, 573, 685, 688
PleI GAGTC 2 cut(s) 323, 780
PluTI GGCGCC 1 cut(s) 515
PpsI GAGTC 2 cut(s) 323, 780
PpuMI RGGWCCY 1 cut(s) 148
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 148
Psp6I CCWGG 2 cut(s) 150, 357
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 2 cut(s) 150, 357
PspN4I GGNNCC 3 cut(s) 481, 513, 558
PspPI GGNCC 4 cut(s) 148, 379, 388, 448
PspPPI RGGWCCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 2 cut(s) 678, 723
RsaNI GTAC 2 cut(s) 677, 722
SacI GAGCTC 1 cut(s) 351
SacII CCGCGG 1 cut(s) 687
SaqAI TTAA 2 cut(s) 251, 645
SatI GCNGC 8 cut(s) 102, 451, 454, 510, 519, 572, 684, 687
Sau96I GGNCC 4 cut(s) 148, 379, 388, 448
ScaI AGTACT 1 cut(s) 723
SchI GAGTC 2 cut(s) 323, 781
ScrFI CCNGG 3 cut(s) 152, 359, 418
SduI GDGCHC 1 cut(s) 351
SfoI GGCGCC 1 cut(s) 513
Sfr303I CCGCGG 1 cut(s) 687
SfuI TTCGAA 1 cut(s) 242
SgrAI CRCCGGYG 1 cut(s) 514
SgrBI CCGCGG 1 cut(s) 687
SinI GGWCC 2 cut(s) 148, 379
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 3 cut(s) 248, 563, 748
SseBI AGGCCT 1 cut(s) 371
SspDI GGCGCC 1 cut(s) 511
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 1 cut(s) 351
StuI AGGCCT 1 cut(s) 371
StyD4I CCNGG 3 cut(s) 150, 357, 416
TaiI ACGT 3 cut(s) 588, 666, 735
TaqI TCGA 6 cut(s) 135, 212, 242, 282, 297, 375
TasI AATT 3 cut(s) 248, 563, 748
TatI WGTACW 1 cut(s) 721
TauI GCSGC 6 cut(s) 453, 456, 512, 521, 686, 689
TfiI GAWTC 1 cut(s) 763
Tru1I TTAA 2 cut(s) 251, 645
Tru9I TTAA 2 cut(s) 251, 645
TscAI CASTG 1 cut(s) 552
TseI GCWGC 2 cut(s) 101, 571
TspDTI ATGAA 3 cut(s) 17, 191, 507
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 1 cut(s) 552
VpaK11BI GGWCC 2 cut(s) 148, 379
XspI CTAG 1 cut(s) 110
ZrmI AGTACT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.