MD13G1122500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013315
Physical Location & Seq
Reverse (-)
9042414 .. 9043256
843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1122500.v1.1.491

Sequence Viewer

Length: 843 bp
ATGAAGATAAAGAACAAGGGCAAAGTCTACCCATCTCCCTCCTCCTCCGTCACCGCCGGCTCCTCCTCCTCCTCCTCCGACGGGTATGTTCTCTCCGTTCTTCAACTCCTCCCAGCGGCCATTCTAGCTCTAGCCTCCGTGCTCTCTCTCGAGGACCGCGAGGTCCTGGCTTACTTGATCACCCGCTCCATGAAAAACACCCCCAACACTTCTTCTTCAATCCCCGCCGCCCAAGACCCCCAGAAAAAGACTTCCAAGAAAGGTCCTAAGAAGTCCGCCAGCACCGCCGCCGCCCATCAGCCTCCAATGTTCGATTGCGACTGCTTTGACTGCTACAGAAGCTACTGGTTCAAGTGGGATTCTTCCCCCAACCGCGAGCTCATCCACCAAGCCATCGAAGCCTTCGAGGACCACTTGGCTAACGGCGAGCGGCCCAAGAGGAACGCCGGCAAAGGCAAGAGAAGAGACAAGCAGGGCCGTCGGGACGCCCCCATTACGGCCGATGTTTCTGCCCAAAATGAAATCTTTCTGGAAGAGAACGCCGTTCCAGCTTCGCCGGAGTTTGTTCAAAACTCTGGTGCCGCCGTGGTGCCGGAAAATGAGGTTCTTTTCGTGGGTTCGCCTGAGAAGGCTGATGGGGTCGAAGTAAATGAGGGGAAAGTGGAAGACTTGGGCGTGGATGTTACGGTAGCGCCGCCGGATACGGCGGATGACATGGCTGTGATTCTGAGGGCGGCGGCGGCGGCGGCGAGCAACCACAGGGGTTTGGCAAGGAAGGTGCTGCCGGACGTGTTGGGCCTCTTTAACTCTCGTTTGTGGAACCTTTGGAGTCCGAATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.16

Weight (kDa)

6.45

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13090
fragaria_vesca FvH4_4g23440
malus_domestica MD05G1113800.v1.1 MD13G1122500.v1.1 MD16G1123000.v1.1
prunus_persica Prupe.1G222700_v2.0.a1 Prupe.6G184300_v2.0.a1
pyrus_communis pycom13g10660
rosa_chinensis RchiOBHm_Chr4g0429791
rosa_laevigata RLG00000007042
rosa_multiflora Rmu_co8022056.1_g000001 Rmu_sc0018457.1_g000001
rosa_roxburghii Rroxscaffold_5G00371570
rosa_rugosa Rorug04G0238400
rosa_samantha Rh4BG300700 Rh4CG316200 Rh4DG297800
rosa_wichuraiana Rw4G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccB1I GGYRCC 2 cut(s) 578, 589
AccBSI CCGCTC 2 cut(s) 186, 430
AccI GTMKAC 1 cut(s) 27
AccII CGCG 2 cut(s) 159, 375
AcoI YGGCCR 2 cut(s) 117, 498
AcyI GRCGYC 1 cut(s) 486
AfiI CCNNNNNNNGG 3 cut(s) 81, 115, 496
AflIII ACRYGT 1 cut(s) 789
AgsI TTSAA 4 cut(s) 104, 219, 352, 569
AjiI CACGTC 1 cut(s) 790
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 4 cut(s) 128, 342, 379, 551
AluI AGCT 4 cut(s) 128, 342, 379, 551
Alw21I GWGCWC 2 cut(s) 144, 381
Alw26I GTCTC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 5 cut(s) 117, 431, 475, 498, 796
ApeKI GCWGC 1 cut(s) 781
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 7 cut(s) 154, 163, 263, 409, 432, 475, 796
AsuHPI GGTGA 2 cut(s) 43, 172
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 4 cut(s) 154, 163, 263, 409
BanI GGYRCC 2 cut(s) 578, 589
BanII GRGCYC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 2 cut(s) 144, 381
BbvI GCAGC 1 cut(s) 768
BccI CCATC 4 cut(s) 40, 303, 401, 629
BceAI ACGGC 6 cut(s) 439, 462, 513, 527, 569, 720
BcgI CGANNNNNNTGC 4 cut(s) 461, 491, 495, 525
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 694
BclI TGATCA 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 2 cut(s) 125, 131
BfmI CTRYAG 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 695
BfuI GTATCC 1 cut(s) 694
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 4 cut(s) 154, 163, 263, 409
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 790
BmgT120I GGNCC 7 cut(s) 154, 163, 263, 409, 432, 475, 796
BmiI GGNNCC 4 cut(s) 61, 580, 591, 821
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 672
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 585
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 115, 496
Bse118I RCCGGY 2 cut(s) 56, 446
Bse1I ACTGG 1 cut(s) 350
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 3 cut(s) 381, 685, 715
BseLI CCNNNNNNNGG 3 cut(s) 81, 115, 496
BseMII CTCAG 2 cut(s) 615, 719
BseNI ACTGG 1 cut(s) 350
BseRI GAGGAG 8 cut(s) 31, 34, 52, 55, 58, 61, 64, 98
BseX3I CGGCCG 1 cut(s) 498
BseXI GCAGC 1 cut(s) 768
BseYI CCCAGC 1 cut(s) 112
Bsh1236I CGCG 2 cut(s) 159, 375
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 5 cut(s) 119, 433, 477, 500, 798
BshNI GGYRCC 2 cut(s) 578, 589
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 144, 381
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 6 cut(s) 57, 447, 557, 593, 698, 785
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 81, 115, 496
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 1 cut(s) 497
BsnI GGCC 5 cut(s) 119, 433, 477, 500, 798
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 144, 381
Bsp143I GATC 1 cut(s) 177
BspANI GGCC 5 cut(s) 119, 433, 477, 500, 798
BspCNI CTCAG 2 cut(s) 616, 720
BspFNI CGCG 2 cut(s) 159, 375
BspLI GGNNCC 4 cut(s) 61, 580, 591, 821
BspT107I GGYRCC 2 cut(s) 578, 589
BsrBI CCGCTC 2 cut(s) 186, 430
BsrFI RCCGGY 2 cut(s) 56, 446
BsrI ACTGG 1 cut(s) 350
BssAI RCCGGY 2 cut(s) 56, 446
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 1 cut(s) 177
BssNI GRCGYC 1 cut(s) 486
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 688
Bst6I CTCTTC 2 cut(s) 457, 528
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 6 cut(s) 58, 280, 377, 428, 448, 751
BstDEI CTNAG 3 cut(s) 267, 624, 728
BstDSI CCRYGG 1 cut(s) 585
BstF5I GGATG 3 cut(s) 381, 685, 715
BstFNI CGCG 2 cut(s) 159, 375
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 1 cut(s) 180
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 1 cut(s) 177
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 9 cut(s) 125, 284, 330, 339, 398, 548, 740, 743, 746
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 334
BstUI CGCG 2 cut(s) 159, 375
BstV1I GCAGC 1 cut(s) 768
BstV2I GAAGAC 1 cut(s) 672
BstZI CGGCCG 1 cut(s) 498
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 5 cut(s) 119, 433, 477, 500, 798
BtgI CCRYGG 1 cut(s) 585
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 3 cut(s) 381, 685, 715
Cac8I GCNNGC 6 cut(s) 58, 280, 377, 428, 448, 751
CfoI GCGC 1 cut(s) 694
Cfr10I RCCGGY 2 cut(s) 56, 446
Cfr13I GGNCC 7 cut(s) 154, 163, 263, 409, 432, 475, 796
CseI GACGC 1 cut(s) 494
CviAII CATG 2 cut(s) 190, 715
DdeI CTNAG 3 cut(s) 267, 624, 728
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
EaeI YGGCCR 2 cut(s) 117, 498
EagI CGGCCG 1 cut(s) 498
Eam1104I CTCTTC 2 cut(s) 457, 528
EarI CTCTTC 2 cut(s) 457, 528
EciI GGCGGA 2 cut(s) 265, 722
Ecl136II GAGCTC 1 cut(s) 379
EclXI CGGCCG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 4 cut(s) 154, 163, 263, 409
Eco52I CGGCCG 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 379
EcoO109I RGGNCCY 2 cut(s) 163, 263
EcoRII CCWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 381
FaeI CATG 2 cut(s) 193, 718
FaiI YATR 3 cut(s) 87, 191, 716
FaqI GGGAC 1 cut(s) 497
FatI CATG 2 cut(s) 189, 714
FauI CCCGC 2 cut(s) 191, 232
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 1 cut(s) 27
FokI GGATG 3 cut(s) 368, 692, 722
FriOI GRGCYC 1 cut(s) 381
FspBI CTAG 2 cut(s) 125, 131
GlaI GCGC 1 cut(s) 693
GsaI CCCAGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 5 cut(s) 119, 433, 477, 500, 798
HapII CCGG 6 cut(s) 57, 447, 557, 593, 698, 785
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 694
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 2 cut(s) 193, 718
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HinfI GANTC 3 cut(s) 359, 724, 829
HpaII CCGG 6 cut(s) 57, 447, 557, 593, 698, 785
HphI GGTGA 2 cut(s) 43, 172
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 79, 729, 834
Hpy188III TCNNGA 3 cut(s) 149, 482, 530
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 2 cut(s) 83, 483
HpyAV CCTTC 3 cut(s) 412, 622, 769
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 1 cut(s) 789
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 284, 330, 339, 398, 548, 740, 743, 746
HpyF3I CTNAG 3 cut(s) 267, 624, 728
HpySE526I ACGT 1 cut(s) 789
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 2 cut(s) 193, 718
HspAI GCGC 1 cut(s) 692
KroI GCCGGC 2 cut(s) 56, 446
KroNI GCCGGC 2 cut(s) 58, 448
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 2 cut(s) 65, 191
Lsp1109I GCAGC 1 cut(s) 768
MaeI CTAG 2 cut(s) 125, 131
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 2 cut(s) 49, 682
MalI GATC 1 cut(s) 179
MbiI CCGCTC 2 cut(s) 186, 430
MboI GATC 1 cut(s) 177
MboII GAAGA 8 cut(s) 16, 92, 204, 207, 354, 474, 545, 677
MhlI GDGCHC 2 cut(s) 144, 381
MlyI GAGTC 1 cut(s) 838
MmeI TCCRAC 1 cut(s) 102
MroNI GCCGGC 2 cut(s) 56, 446
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 1 cut(s) 804
MslI CAYNNNNRTG 1 cut(s) 719
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 6 cut(s) 57, 447, 557, 593, 698, 785
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 2 cut(s) 159, 375
MwoI GCNNNNNNNGC 9 cut(s) 125, 284, 330, 339, 398, 548, 740, 743, 746
NaeI GCCGGC 2 cut(s) 58, 448
NdeII GATC 1 cut(s) 177
NgoMIV GCCGGC 2 cut(s) 56, 446
NlaIII CATG 2 cut(s) 193, 718
NlaIV GGNNCC 4 cut(s) 61, 580, 591, 821
NmuCI GTSAC 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 149
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 402
PdiI GCCGGC 2 cut(s) 58, 448
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 359, 724
PleI GAGTC 1 cut(s) 837
PpsI GAGTC 1 cut(s) 837
PpuMI RGGWCCY 2 cut(s) 163, 263
Psp124BI GAGCTC 1 cut(s) 381
Psp5II RGGWCCY 2 cut(s) 163, 263
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 4 cut(s) 61, 580, 591, 821
PspPI GGNCC 7 cut(s) 154, 163, 263, 409, 432, 475, 796
PspPPI RGGWCCY 2 cut(s) 163, 263
RseI CAYNNNNRTG 1 cut(s) 719
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 1 cut(s) 804
Sau3AI GATC 1 cut(s) 177
Sau96I GGNCC 7 cut(s) 154, 163, 263, 409, 432, 475, 796
SchI GAGTC 1 cut(s) 838
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 2 cut(s) 144, 381
SfcI CTRYAG 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 149
SinI GGWCC 4 cut(s) 154, 163, 263, 409
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 719
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SspMI CTAG 2 cut(s) 125, 131
SstI GAGCTC 1 cut(s) 381
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 1 cut(s) 792
TaqI TCGA 5 cut(s) 150, 312, 396, 405, 642
TfiI GAWTC 2 cut(s) 359, 724
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 1 cut(s) 781
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 3 cut(s) 17, 206, 534
TspGWI ACGGA 3 cut(s) 37, 85, 127
VpaK11BI GGWCC 4 cut(s) 154, 163, 263, 409
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 2 cut(s) 125, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.