pycom13g10660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013315
Physical Location & Seq
Reverse (-)
7165144 .. 7165980
837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10660.1

Sequence Viewer

Length: 837 bp
ATGAAGATAAAGAACAAGGGCAAAGTCTACCCATCTCCCTCCTCCTCCATCCCCGCCGCCTCCTCCTCCTCCGACGGGTATGTTCTCTCCGTTCTTCAACTCCTCCCAGCGGCCATTCTAGCTCTAGCCTCCGTCCTCTCTCTCGAGGACCGCGAGGTCCTGGCTTACTTGATTACCCGCTCCATGAAAAACACCCCCAACACTTCTTCGTCTTCAATCCCCGCCGCACAAGACCCCCAGAAAAAGACTTCCAAGAAAGGTCCTAAGAAGTCCGCCAGCACCGCCGCCGCCCATCAGCCTCCAATGTTCGATTGCGACTGCTTTGACTGCTACAGAAGCTACTGGTTCAAGTGGGACTCTTCCCCCAACCGCGAGCTCATCCACCAAGCCATCGAAGCCTTCGAGGACCACTTGGCTAACGGCGAGCGGCCCAAGAGGAACGCTGGCAGAGGCAAGAGAAGAGACAAGCAGGGCCGTCGGGACGCCCCCATTACGGCCGATGTTTCTGCCCAAAATGAAATCTTTCTGGAAGAGAACGCCGTTCCAGCTTCGCCGGAGTTTGTTCAAAACTCTGGTGCCGCCGTGGTGCCGGAAAATGAGGTTCTTTTCTTGGGTTCGCCTGAGAAGGCTGAAGGGGTCGAAGTAAATGAGGGGAAAGTGGAAGACTTGGGCGTGGATGTTACGGTAGCGCCGCCGGATACGGTGGATGACATGGCTGTGATTCTGAGGGGGGCGGCGGCGGCGAGCAGCCACAGGGGTTTGGCAAGGAAAGTGCTGCCGGACGTGTTGGGCCTCTTTAACTCTCGTTTGTGGAACCTTTGGAGTCCGAATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

30.07

Weight (kDa)

6.46

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13090
fragaria_vesca FvH4_4g23440
malus_domestica MD05G1113800.v1.1 MD13G1122500.v1.1 MD16G1123000.v1.1
prunus_persica Prupe.1G222700_v2.0.a1 Prupe.6G184300_v2.0.a1
pyrus_communis pycom13g10660
rosa_chinensis RchiOBHm_Chr4g0429791
rosa_laevigata RLG00000007042
rosa_multiflora Rmu_co8022056.1_g000001 Rmu_sc0018457.1_g000001
rosa_roxburghii Rroxscaffold_5G00371570
rosa_rugosa Rorug04G0238400
rosa_samantha Rh4BG300700 Rh4CG316200 Rh4DG297800
rosa_wichuraiana Rw4G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 155
AccB1I GGYRCC 2 cut(s) 575, 586
AccBSI CCGCTC 2 cut(s) 180, 427
AccI GTMKAC 1 cut(s) 27
AccII CGCG 2 cut(s) 153, 372
AcoI YGGCCR 2 cut(s) 111, 495
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 483
AfiI CCNNNNNNNGG 3 cut(s) 75, 109, 493
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 4 cut(s) 98, 216, 349, 566
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 4 cut(s) 122, 339, 376, 548
AluI AGCT 4 cut(s) 122, 339, 376, 548
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 456
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 5 cut(s) 111, 428, 472, 495, 790
ApeKI GCWGC 2 cut(s) 747, 775
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 1 cut(s) 691
AspS9I GGNCC 7 cut(s) 148, 157, 260, 406, 429, 472, 790
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 4 cut(s) 148, 157, 260, 406
BanI GGYRCC 2 cut(s) 575, 586
BanII GRGCYC 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 204, 669
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 759, 762
BccI CCATC 4 cut(s) 40, 56, 300, 398
BceAI ACGGC 5 cut(s) 436, 459, 510, 524, 566
BcgI CGANNNNNNTGC 4 cut(s) 458, 488, 492, 522
BciT130I CCWGG 1 cut(s) 161
BciVI GTATCC 1 cut(s) 691
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 2 cut(s) 119, 125
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 1 cut(s) 692
BfuI GTATCC 1 cut(s) 691
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 4 cut(s) 148, 157, 260, 406
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 784
BmgT120I GGNCC 7 cut(s) 148, 157, 260, 406, 429, 472, 790
BmiI GGNNCC 3 cut(s) 577, 588, 815
BmrFI CCNGG 1 cut(s) 161
BpiI GAAGAC 2 cut(s) 204, 669
BsaHI GRCGYC 1 cut(s) 483
BsaJI CCNNGG 1 cut(s) 582
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 109, 493
Bse1I ACTGG 1 cut(s) 347
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 4 cut(s) 48, 378, 682, 712
BseLI CCNNNNNNNGG 3 cut(s) 75, 109, 493
BseMII CTCAG 2 cut(s) 612, 716
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 6 cut(s) 31, 34, 52, 55, 58, 92
BseX3I CGGCCG 1 cut(s) 495
BseXI GCAGC 2 cut(s) 759, 762
BseYI CCCAGC 1 cut(s) 106
Bsh1236I CGCG 2 cut(s) 153, 372
Bsh1285I CGRYCG 1 cut(s) 498
BshFI GGCC 5 cut(s) 113, 430, 474, 497, 792
BshNI GGYRCC 2 cut(s) 575, 586
BsiEI CGRYCG 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 4 cut(s) 554, 590, 695, 779
BslFI GGGAC 2 cut(s) 368, 494
BslI CCNNNNNNNGG 3 cut(s) 75, 109, 493
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 2 cut(s) 368, 494
BsnI GGCC 5 cut(s) 113, 430, 474, 497, 792
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 378
BspANI GGCC 5 cut(s) 113, 430, 474, 497, 792
BspCNI CTCAG 2 cut(s) 613, 717
BspFNI CGCG 2 cut(s) 153, 372
BspLI GGNNCC 3 cut(s) 577, 588, 815
BspT107I GGYRCC 2 cut(s) 575, 586
BsrBI CCGCTC 2 cut(s) 180, 427
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 582
BssNI GRCGYC 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 2 cut(s) 685, 703
Bst6I CTCTTC 3 cut(s) 364, 454, 525
BstACI GRCGYC 1 cut(s) 483
BstC8I GCNNGC 5 cut(s) 277, 374, 425, 445, 745
BstDEI CTNAG 3 cut(s) 264, 621, 725
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 4 cut(s) 48, 378, 682, 712
BstFNI CGCG 2 cut(s) 153, 372
BstH2I RGCGCY 1 cut(s) 692
BstHHI GCGC 1 cut(s) 691
BstMAI GTCTC 1 cut(s) 456
BstMCI CGRYCG 1 cut(s) 498
BstMWI GCNNNNNNNGC 7 cut(s) 119, 281, 327, 336, 395, 545, 740
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 331
BstUI CGCG 2 cut(s) 153, 372
BstV1I GCAGC 2 cut(s) 759, 762
BstV2I GAAGAC 2 cut(s) 204, 669
BstZI CGGCCG 1 cut(s) 495
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 5 cut(s) 113, 430, 474, 497, 792
BtgI CCRYGG 1 cut(s) 582
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 4 cut(s) 48, 378, 682, 712
Cac8I GCNNGC 5 cut(s) 277, 374, 425, 445, 745
CfoI GCGC 1 cut(s) 691
Cfr13I GGNCC 7 cut(s) 148, 157, 260, 406, 429, 472, 790
CseI GACGC 1 cut(s) 491
CviAII CATG 2 cut(s) 184, 712
DdeI CTNAG 3 cut(s) 264, 621, 725
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 2 cut(s) 111, 495
EagI CGGCCG 1 cut(s) 495
Eam1104I CTCTTC 3 cut(s) 364, 454, 525
EarI CTCTTC 3 cut(s) 364, 454, 525
EciI GGCGGA 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 376
EclXI CGGCCG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 4 cut(s) 148, 157, 260, 406
Eco52I CGGCCG 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 376
EcoO109I RGGNCCY 2 cut(s) 157, 260
EcoRII CCWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 378
FaeI CATG 2 cut(s) 187, 715
FaiI YATR 3 cut(s) 81, 185, 713
FaqI GGGAC 2 cut(s) 368, 494
FatI CATG 2 cut(s) 183, 711
FauI CCCGC 3 cut(s) 61, 185, 229
FblI GTMKAC 1 cut(s) 27
FokI GGATG 4 cut(s) 35, 365, 689, 719
FriOI GRGCYC 1 cut(s) 378
FspBI CTAG 2 cut(s) 119, 125
GlaI GCGC 1 cut(s) 690
GsaI CCCAGC 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 692
HaeIII GGCC 5 cut(s) 113, 430, 474, 497, 792
HapII CCGG 4 cut(s) 554, 590, 695, 779
HgaI GACGC 1 cut(s) 491
HhaI GCGC 1 cut(s) 691
Hin1I GRCGYC 1 cut(s) 483
Hin1II CATG 2 cut(s) 187, 715
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HinfI GANTC 3 cut(s) 356, 721, 823
HpaII CCGG 4 cut(s) 554, 590, 695, 779
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 73, 726, 828
Hpy188III TCNNGA 3 cut(s) 143, 479, 527
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 2 cut(s) 77, 480
HpyAV CCTTC 3 cut(s) 409, 619, 626
HpyCH4III ACNGT 2 cut(s) 685, 703
HpyCH4IV ACGT 1 cut(s) 783
HpyF10VI GCNNNNNNNGC 7 cut(s) 119, 281, 327, 336, 395, 545, 740
HpyF3I CTNAG 3 cut(s) 264, 621, 725
HpySE526I ACGT 1 cut(s) 783
Hsp92I GRCGYC 1 cut(s) 483
Hsp92II CATG 2 cut(s) 187, 715
HspAI GCGC 1 cut(s) 689
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 2 cut(s) 759, 762
MaeI CTAG 2 cut(s) 119, 125
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 1 cut(s) 679
MbiI CCGCTC 2 cut(s) 180, 427
MboII GAAGA 8 cut(s) 16, 86, 198, 204, 351, 471, 542, 674
MhlI GDGCHC 1 cut(s) 378
MlyI GAGTC 2 cut(s) 350, 832
MmeI TCCRAC 1 cut(s) 96
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 1 cut(s) 798
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 4 cut(s) 554, 590, 695, 779
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 2 cut(s) 153, 372
MwoI GCNNNNNNNGC 7 cut(s) 119, 281, 327, 336, 395, 545, 740
NlaIII CATG 2 cut(s) 187, 715
NlaIV GGNNCC 3 cut(s) 577, 588, 815
PaeR7I CTCGAG 1 cut(s) 143
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 399
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 721
PleI GAGTC 2 cut(s) 350, 831
PpsI GAGTC 2 cut(s) 350, 831
PpuMI RGGWCCY 2 cut(s) 157, 260
Psp124BI GAGCTC 1 cut(s) 378
Psp5II RGGWCCY 2 cut(s) 157, 260
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 577, 588, 815
PspPI GGNCC 7 cut(s) 148, 157, 260, 406, 429, 472, 790
PspPPI RGGWCCY 2 cut(s) 157, 260
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 1 cut(s) 798
Sau96I GGNCC 7 cut(s) 148, 157, 260, 406, 429, 472, 790
SchI GAGTC 2 cut(s) 350, 832
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 1 cut(s) 378
SetI ASST 9 cut(s) 124, 159, 262, 341, 378, 550, 603, 786, 819
SfcI CTRYAG 1 cut(s) 331
Sfr274I CTCGAG 1 cut(s) 143
SinI GGWCC 4 cut(s) 148, 157, 260, 406
SlaI CTCGAG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SspMI CTAG 2 cut(s) 119, 125
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 685, 703
TaiI ACGT 1 cut(s) 786
TaqI TCGA 5 cut(s) 144, 309, 393, 402, 639
TfiI GAWTC 1 cut(s) 721
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TseI GCWGC 2 cut(s) 747, 775
TspDTI ATGAA 3 cut(s) 17, 200, 531
TspGWI ACGGA 2 cut(s) 79, 121
VpaK11BI GGWCC 4 cut(s) 148, 157, 260, 406
XhoI CTCGAG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 2 cut(s) 119, 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.