Rw4G025480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013315
Physical Location & Seq
Forward (+)
51595592 .. 51597095
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025480.1

Sequence Viewer

Length: 813 bp
ATGAAGATAAAGAACAAAGGCAAAGTCCACCCATCTCCTTCCTCATCTTCCTCTTCCTCTTCCTCCTCCTCCTCCTCATCTTCTTCCGATGGAGTTGTTCTCTCCGTGCTCCAGCTACTCCCAGCTGCTATTCTAGCTCTGGCCACGGTTCTCTCCCTTGAGGACCGCGAGGTGTTGGCTTACATGATAACCCGTTCCATGAAAACCGCACCCTCTTTTTCTTTCTCAGACACCACCCAAGATTCCAAGAAGAAATCTATGAGGAAATCTAGTAGTAAAGCCGGCAGGACTCAGAGCCAGAGCAACAACCACCAGCCGCCGATGTTCGATTGCGAGTGCTTCGACTGCTACAGAAGCTACTGGTTTAAGTGGGACTCCTCCCCCAACCGCGAGCTCATCCACCAGGCCATTGAGGCCTTCGAGGACCACCTGGCCCACGGTGAGAAGCCCAAGAAGAACGCCGGCAGGGGGAAGCGAAGAGACAAGATGGGCCGCCGCGAGGCCCAAAAATCAGCCGATAATTCCTCCAAAGATGAAGTCTTTGTGGCGGAGGAGAATGTAGGGAGCTCAGTTGCCGAGGTGGTGTTACCTCTGTGCGACGTCGTTTCGGTGGGGTCCCCGGAGAAAGTTGAGGAGTTGGAAGTGGAAAAGCAAGCTGTGGAGGTTAAGACGGAGACGACGGCGGACGACGTGGCGTTGGTTGTACGCGCCGCGGCGGTGAGCAACCACAAGGGCCTGGCCAGGAAAGTACTTCCGGACGTGTTGGGGCTATTCAATTCTCGTTTGTGGAATCTTTGGAGTCCGAATGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

29.55

Weight (kDa)

8.58

Isoelectric Point (pI)

63.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13090
fragaria_vesca FvH4_4g23440
malus_domestica MD05G1113800.v1.1 MD13G1122500.v1.1 MD16G1123000.v1.1
prunus_persica Prupe.1G222700_v2.0.a1 Prupe.6G184300_v2.0.a1
pyrus_communis pycom13g10660
rosa_chinensis RchiOBHm_Chr4g0429791
rosa_laevigata RLG00000007042
rosa_multiflora Rmu_co8022056.1_g000001 Rmu_sc0018457.1_g000001
rosa_roxburghii Rroxscaffold_5G00371570
rosa_rugosa Rorug04G0238400
rosa_samantha Rh4BG300700 Rh4CG316200 Rh4DG297800
rosa_wichuraiana Rw4G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 603
AccII CGCG 5 cut(s) 168, 390, 498, 708, 713
AccIII TCCGGA 1 cut(s) 754
AcoI YGGCCR 2 cut(s) 141, 738
AcyI GRCGYC 1 cut(s) 600
AfaI GTAC 2 cut(s) 705, 750
AfiI CCNNNNNNNGG 2 cut(s) 468, 499
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 1 cut(s) 775
AjiI CACGTC 2 cut(s) 691, 760
AjnI CCWGG 4 cut(s) 402, 429, 735, 740
AluBI AGCT 7 cut(s) 115, 125, 137, 357, 394, 567, 656
AluI AGCT 7 cut(s) 115, 125, 137, 357, 394, 567, 656
Alw21I GWGCWC 3 cut(s) 111, 396, 569
Alw26I GTCTC 2 cut(s) 474, 668
Aor13HI TCCGGA 1 cut(s) 754
AoxI GGCC 8 cut(s) 141, 405, 414, 432, 490, 501, 733, 738
ApeKI GCWGC 1 cut(s) 125
ArsI GACNNNNNNTTYG 4 cut(s) 9, 41, 522, 554
AspLEI GCGC 1 cut(s) 710
AspS9I GGNCC 7 cut(s) 163, 424, 433, 490, 502, 615, 733
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 2 cut(s) 452, 730
AvaII GGWCC 3 cut(s) 163, 424, 615
BalI TGGCCA 2 cut(s) 143, 740
BanII GRGCYC 2 cut(s) 396, 569
Bbv12I GWGCWC 3 cut(s) 111, 396, 569
BbvI GCAGC 1 cut(s) 112
BccI CCATC 3 cut(s) 40, 83, 481
BceAI ACGGC 1 cut(s) 696
BciT130I CCWGG 4 cut(s) 404, 431, 737, 742
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 2 cut(s) 474, 668
BfaI CTAG 2 cut(s) 134, 270
BfmI CTRYAG 1 cut(s) 349
BglI GCCNNNNNGGC 1 cut(s) 413
BisI GCNGC 6 cut(s) 126, 317, 493, 496, 711, 714
BlsI GCNGC 6 cut(s) 127, 318, 494, 497, 712, 715
BmcAI AGTACT 1 cut(s) 750
Bme1390I CCNGG 5 cut(s) 404, 431, 620, 737, 742
Bme18I GGWCC 3 cut(s) 163, 424, 615
BmgBI CACGTC 2 cut(s) 691, 760
BmgT120I GGNCC 7 cut(s) 163, 424, 433, 490, 502, 615, 733
BmiI GGNNCC 2 cut(s) 616, 617
BmrFI CCNGG 5 cut(s) 404, 431, 620, 737, 742
BplI GAGNNNNNCTC 2 cut(s) 84, 116
BpmI CTGGAG 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 600
BsaJI CCNNGG 5 cut(s) 144, 436, 576, 618, 711
BsaWI WCCGGW 1 cut(s) 754
Bsc4I CCNNNNNNNGG 2 cut(s) 468, 499
Bse118I RCCGGY 2 cut(s) 281, 461
Bse1I ACTGG 1 cut(s) 365
BseAI TCCGGA 1 cut(s) 754
BseBI CCWGG 4 cut(s) 404, 431, 737, 742
BseDI CCNNGG 5 cut(s) 144, 436, 576, 618, 711
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 2 cut(s) 468, 499
BseMII CTCAG 3 cut(s) 240, 305, 582
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 7 cut(s) 55, 58, 61, 64, 367, 566, 647
BseXI GCAGC 1 cut(s) 112
BseYI CCCAGC 1 cut(s) 121
Bsh1236I CGCG 5 cut(s) 168, 390, 498, 708, 713
BshFI GGCC 8 cut(s) 143, 407, 416, 434, 492, 503, 735, 740
BsiHKAI GWGCWC 3 cut(s) 111, 396, 569
BsiSI CCGG 4 cut(s) 282, 462, 620, 755
BslFI GGGAC 2 cut(s) 386, 601
BslI CCNNNNNNNGG 2 cut(s) 468, 499
BsmAI GTCTC 2 cut(s) 474, 668
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 2 cut(s) 386, 601
BsmI GAATGC 1 cut(s) 811
BsnI GGCC 8 cut(s) 143, 407, 416, 434, 492, 503, 735, 740
Bsp1286I GDGCHC 3 cut(s) 111, 396, 569
Bsp13I TCCGGA 1 cut(s) 754
BspANI GGCC 8 cut(s) 143, 407, 416, 434, 492, 503, 735, 740
BspCNI CTCAG 3 cut(s) 239, 304, 581
BspEI TCCGGA 1 cut(s) 754
BspFNI CGCG 5 cut(s) 168, 390, 498, 708, 713
BspLI GGNNCC 2 cut(s) 616, 617
BsrFI RCCGGY 2 cut(s) 281, 461
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 2 cut(s) 281, 461
BssECI CCNNGG 5 cut(s) 144, 436, 576, 618, 711
BssNI GRCGYC 1 cut(s) 600
Bst2UI CCWGG 4 cut(s) 404, 431, 737, 742
Bst4CI ACNGT 2 cut(s) 148, 440
Bst6I CTCTTC 3 cut(s) 58, 64, 472
BstACI GRCGYC 1 cut(s) 600
BstC8I GCNNGC 4 cut(s) 283, 392, 463, 654
BstDEI CTNAG 3 cut(s) 226, 291, 568
BstDSI CCRYGG 3 cut(s) 144, 436, 711
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 5 cut(s) 168, 390, 498, 708, 713
BstHHI GCGC 1 cut(s) 710
BstMAI GTCTC 2 cut(s) 474, 668
BstMWI GCNNNNNNNGC 4 cut(s) 134, 345, 354, 413
BstNI CCWGG 4 cut(s) 404, 431, 737, 742
BstSCI CCNGG 5 cut(s) 402, 429, 618, 735, 740
BstSFI CTRYAG 1 cut(s) 349
BstUI CGCG 5 cut(s) 168, 390, 498, 708, 713
BstV1I GCAGC 1 cut(s) 112
BsuRI GGCC 8 cut(s) 143, 407, 416, 434, 492, 503, 735, 740
BtgI CCRYGG 3 cut(s) 144, 436, 711
BtrI CACGTC 2 cut(s) 691, 760
BtsCI GGATG 1 cut(s) 396
Cac8I GCNNGC 4 cut(s) 283, 392, 463, 654
CfoI GCGC 1 cut(s) 710
Cfr10I RCCGGY 2 cut(s) 281, 461
Cfr13I GGNCC 7 cut(s) 163, 424, 433, 490, 502, 615, 733
Cfr42I CCGCGG 1 cut(s) 714
Csp6I GTAC 2 cut(s) 704, 749
CviAII CATG 2 cut(s) 184, 199
CviQI GTAC 2 cut(s) 704, 749
DdeI CTNAG 3 cut(s) 226, 291, 568
EaeI YGGCCR 2 cut(s) 141, 738
Eam1104I CTCTTC 3 cut(s) 58, 64, 472
EarI CTCTTC 3 cut(s) 58, 64, 472
EciI GGCGGA 2 cut(s) 563, 698
Ecl136II GAGCTC 2 cut(s) 394, 567
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 2 cut(s) 396, 569
Eco47I GGWCC 3 cut(s) 163, 424, 615
Eco53kI GAGCTC 2 cut(s) 394, 567
EcoICRI GAGCTC 2 cut(s) 394, 567
EcoO109I RGGNCCY 2 cut(s) 615, 733
EcoRII CCWGG 4 cut(s) 402, 429, 735, 740
EcoT38I GRGCYC 2 cut(s) 396, 569
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 2 cut(s) 187, 202
FaiI YATR 3 cut(s) 185, 200, 260
FaqI GGGAC 2 cut(s) 386, 601
FatI CATG 2 cut(s) 183, 198
Fnu4HI GCNGC 6 cut(s) 126, 317, 493, 496, 711, 714
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 2 cut(s) 396, 569
Fsp4HI GCNGC 6 cut(s) 126, 317, 493, 496, 711, 714
FspBI CTAG 2 cut(s) 134, 270
GlaI GCGC 1 cut(s) 709
GluI GCNGC 6 cut(s) 126, 317, 493, 496, 711, 714
GsaI CCCAGC 1 cut(s) 125
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 8 cut(s) 143, 407, 416, 434, 492, 503, 735, 740
HapII CCGG 4 cut(s) 282, 462, 620, 755
HhaI GCGC 1 cut(s) 710
Hin1I GRCGYC 1 cut(s) 600
Hin1II CATG 2 cut(s) 187, 202
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HinfI GANTC 5 cut(s) 242, 289, 374, 790, 799
HpaII CCGG 4 cut(s) 282, 462, 620, 755
HphI GGTGA 2 cut(s) 452, 730
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 4 cut(s) 88, 229, 294, 804
Hpy188III TCNNGA 1 cut(s) 755
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 4 cut(s) 602, 605, 682, 692
HpyAV CCTTC 2 cut(s) 48, 427
HpyCH4III ACNGT 2 cut(s) 148, 440
HpyCH4IV ACGT 3 cut(s) 600, 690, 759
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 345, 354, 413
HpyF3I CTNAG 3 cut(s) 226, 291, 568
HpySE526I ACGT 3 cut(s) 600, 690, 759
Hsp92I GRCGYC 1 cut(s) 600
Hsp92II CATG 2 cut(s) 187, 202
HspAI GCGC 1 cut(s) 708
KflI GGGWCCC 1 cut(s) 615
Kpn2I TCCGGA 1 cut(s) 754
KroI GCCGGC 2 cut(s) 281, 461
KroNI GCCGGC 2 cut(s) 283, 463
KspI CCGCGG 1 cut(s) 714
LmnI GCTCC 2 cut(s) 114, 564
Lsp1109I GCAGC 1 cut(s) 112
MaeI CTAG 2 cut(s) 134, 270
MaeII ACGT 3 cut(s) 600, 690, 759
MaeIII GTNAC 1 cut(s) 585
MboII GAAGA 9 cut(s) 16, 39, 45, 51, 72, 75, 262, 466, 489
MhlI GDGCHC 3 cut(s) 111, 396, 569
MlsI TGGCCA 2 cut(s) 143, 740
MluCI AATT 2 cut(s) 520, 775
MluNI TGGCCA 2 cut(s) 143, 740
MlyI GAGTC 3 cut(s) 283, 368, 808
MmeI TCCRAC 1 cut(s) 618
Mox20I TGGCCA 2 cut(s) 143, 740
MroI TCCGGA 1 cut(s) 754
MroNI GCCGGC 2 cut(s) 281, 461
MscI TGGCCA 2 cut(s) 143, 740
MseI TTAA 2 cut(s) 366, 666
Msp20I TGGCCA 2 cut(s) 143, 740
MspA1I CMGCKG 2 cut(s) 125, 713
MspI CCGG 4 cut(s) 282, 462, 620, 755
MspR9I CCNGG 5 cut(s) 404, 431, 620, 737, 742
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 4 cut(s) 404, 431, 737, 742
MvnI CGCG 5 cut(s) 168, 390, 498, 708, 713
MwoI GCNNNNNNNGC 4 cut(s) 134, 345, 354, 413
NaeI GCCGGC 2 cut(s) 283, 463
NciI CCSGG 1 cut(s) 620
NgoMIV GCCGGC 2 cut(s) 281, 461
NlaIII CATG 2 cut(s) 187, 202
NlaIV GGNNCC 2 cut(s) 616, 617
NmeAIII GCCGAG 1 cut(s) 601
PceI AGGCCT 1 cut(s) 416
PctI GAATGC 1 cut(s) 811
PdiI GCCGGC 2 cut(s) 283, 463
PfeI GAWTC 2 cut(s) 242, 790
PkrI GCNGC 6 cut(s) 127, 318, 494, 497, 712, 715
PleI GAGTC 3 cut(s) 283, 368, 807
PpsI GAGTC 3 cut(s) 283, 368, 807
PpuMI RGGWCCY 1 cut(s) 615
Psp124BI GAGCTC 2 cut(s) 396, 569
Psp5II RGGWCCY 1 cut(s) 615
Psp6I CCWGG 4 cut(s) 402, 429, 735, 740
PspFI CCCAGC 1 cut(s) 121
PspGI CCWGG 4 cut(s) 402, 429, 735, 740
PspN4I GGNNCC 2 cut(s) 616, 617
PspPI GGNCC 7 cut(s) 163, 424, 433, 490, 502, 615, 733
PspPPI RGGWCCY 1 cut(s) 615
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 2 cut(s) 705, 750
RsaNI GTAC 2 cut(s) 704, 749
SacI GAGCTC 2 cut(s) 396, 569
SacII CCGCGG 1 cut(s) 714
SaqAI TTAA 2 cut(s) 366, 666
SatI GCNGC 6 cut(s) 126, 317, 493, 496, 711, 714
Sau96I GGNCC 7 cut(s) 163, 424, 433, 490, 502, 615, 733
ScaI AGTACT 1 cut(s) 750
SchI GAGTC 3 cut(s) 283, 368, 808
ScrFI CCNGG 5 cut(s) 404, 431, 620, 737, 742
SduI GDGCHC 3 cut(s) 111, 396, 569
SfcI CTRYAG 1 cut(s) 349
SfiI GGCCNNNNNGGCC 1 cut(s) 413
Sfr303I CCGCGG 1 cut(s) 714
SgrBI CCGCGG 1 cut(s) 714
SinI GGWCC 3 cut(s) 163, 424, 615
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 2 cut(s) 520, 775
SseBI AGGCCT 1 cut(s) 416
SspMI CTAG 2 cut(s) 134, 270
SstI GAGCTC 2 cut(s) 396, 569
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 5 cut(s) 402, 429, 618, 735, 740
TaaI ACNGT 2 cut(s) 148, 440
TaiI ACGT 3 cut(s) 603, 693, 762
TaqI TCGA 3 cut(s) 327, 342, 420
TasI AATT 2 cut(s) 520, 775
TatI WGTACW 1 cut(s) 748
TauI GCSGC 5 cut(s) 319, 495, 498, 713, 716
TfiI GAWTC 2 cut(s) 242, 790
Tru1I TTAA 2 cut(s) 366, 666
Tru9I TTAA 2 cut(s) 366, 666
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 17, 215, 549
TspGWI ACGGA 2 cut(s) 94, 686
VpaK11BI GGWCC 3 cut(s) 163, 424, 615
XspI CTAG 2 cut(s) 134, 270
ZraI GACGTC 1 cut(s) 601
ZrmI AGTACT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.