MD16G1123000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013315
Physical Location & Seq
Reverse (-)
8881109 .. 8881951
843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1123000.v1.1.491

Sequence Viewer

Length: 843 bp
ATGAAGATAAAGAACAAAGGCAAAGTCCACCCATCTCCCTTCTCCTCCTCCGCCGCCCCCTCCTCTTCCTCCTCCTCCTCCTCGGACGGGTATGTTCTCTCCGTTCTTCAGCTCCTTCCAGCTGCCATTCTAGCTCTGGCCTCCGTCCTCTCTCTCGAGGACCGCGAGGTCCTCGCTTACTTGATTACCCGCTCCATGAAAACCACCCCCAACACTTCTTCTTCTTCTTCTACTTCAATCCCCGCCGCCCAAGACCCCAAGAAAAAGACTTCCAAAAAAGGTTCTAATAAGTCTGCCAGCACCCCTGCCGCCGCCCACCAGCCTCCGATGTTCGATTGCGACTGCTTCGACTGCTACAGAAGCTACTGGTTCAAATGGGACTCTTCCCCCAACCGCGAGCTCATCCACCAAGCCATCGAAGCCTTCGAGGACCACTTGGCCAACGGCGAGCGGCCCAAGAGGAACGCCCGAGGCAAGAGAAGAGACAAGATGGGCCGTCGGGACTCCCCCAAAACGCCCGATGTTTCTGCCCAAAACGATATCTTTCTGGAAGAGAGCGTCGCTCCAGCTTCACCGGAGTTTGTTCAAAGCTCTACTACCGATGTGCTGCCTGAAATCGACGTTGTTTCCGTAGGTTCGCCTGAGAAGGCTGATGCGGTAGAAGGAAATGAGGGGAAAGTGGAAGACTTGGGCGTGGATGTTGCGGTAGCGCCGCCAGATACGGCGGTGGTTTTGAGGGCGGCGGCCGCGAGCAACCACAGGGGTTTGGCAAGGAAAGTGCTGCCGGACGTGTTGGGCCTCTTTAACTCTCGTTTGTGGAACCTTTGGAGTCCGAATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

30.15

Weight (kDa)

7.71

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13090
fragaria_vesca FvH4_4g23440
malus_domestica MD05G1113800.v1.1 MD13G1122500.v1.1 MD16G1123000.v1.1
prunus_persica Prupe.1G222700_v2.0.a1 Prupe.6G184300_v2.0.a1
pyrus_communis pycom13g10660
rosa_chinensis RchiOBHm_Chr4g0429791
rosa_laevigata RLG00000007042
rosa_multiflora Rmu_co8022056.1_g000001 Rmu_sc0018457.1_g000001
rosa_roxburghii Rroxscaffold_5G00371570
rosa_rugosa Rorug04G0238400
rosa_samantha Rh4BG300700 Rh4CG316200 Rh4DG297800
rosa_wichuraiana Rw4G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AccBSI CCGCTC 2 cut(s) 192, 451
AccII CGCG 3 cut(s) 165, 396, 749
AcoI YGGCCR 2 cut(s) 438, 744
AcuI CTGAAG 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 87
AflIII ACRYGT 1 cut(s) 789
AgsI TTSAA 3 cut(s) 237, 373, 587
AjiI CACGTC 1 cut(s) 790
AluBI AGCT 7 cut(s) 112, 122, 134, 363, 400, 569, 591
AluI AGCT 7 cut(s) 112, 122, 134, 363, 400, 569, 591
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 1 cut(s) 477
Ama87I CYCGRG 2 cut(s) 155, 468
AoxI GGCC 6 cut(s) 138, 438, 452, 493, 744, 796
ApeKI GCWGC 3 cut(s) 122, 607, 781
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 6 cut(s) 160, 169, 430, 453, 493, 796
AsuHPI GGTGA 1 cut(s) 564
AvaI CYCGRG 2 cut(s) 155, 468
AvaII GGWCC 3 cut(s) 160, 169, 430
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BalI TGGCCA 1 cut(s) 440
BanII GRGCYC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 690
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 3 cut(s) 109, 594, 768
BccI CCATC 3 cut(s) 40, 422, 484
BceAI ACGGC 3 cut(s) 460, 480, 738
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BcoDI GTCTC 1 cut(s) 477
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 713
Bme18I GGWCC 3 cut(s) 160, 169, 430
BmeT110I CYCGRG 2 cut(s) 155, 468
BmgBI CACGTC 1 cut(s) 790
BmgT120I GGNCC 6 cut(s) 160, 169, 430, 453, 493, 796
BmiI GGNNCC 1 cut(s) 821
BmsI GCATC 1 cut(s) 643
BpiI GAAGAC 1 cut(s) 690
BplI GAGNNNNNCTC 2 cut(s) 547, 579
BpmI CTGGAG 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 81, 469
BsaWI WCCGGW 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 371
BseDI CCNNGG 2 cut(s) 81, 469
BseGI GGATG 2 cut(s) 402, 703
BseLI CCNNNNNNNGG 1 cut(s) 87
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 7 cut(s) 34, 37, 52, 61, 64, 67, 70
BseX3I CGGCCG 1 cut(s) 744
BseXI GCAGC 3 cut(s) 109, 594, 768
Bsh1236I CGCG 3 cut(s) 165, 396, 749
Bsh1285I CGRYCG 1 cut(s) 747
BshFI GGCC 6 cut(s) 140, 440, 454, 495, 746, 798
BsiEI CGRYCG 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 2 cut(s) 155, 468
BsiSI CCGG 2 cut(s) 575, 785
BslFI GGGAC 2 cut(s) 392, 515
BslI CCNNNNNNNGG 1 cut(s) 87
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 2 cut(s) 392, 515
BsnI GGCC 6 cut(s) 140, 440, 454, 495, 746, 798
BsoBI CYCGRG 2 cut(s) 155, 468
Bsp1286I GDGCHC 1 cut(s) 402
BspANI GGCC 6 cut(s) 140, 440, 454, 495, 746, 798
BspCNI CTCAG 1 cut(s) 634
BspFNI CGCG 3 cut(s) 165, 396, 749
BspLI GGNNCC 1 cut(s) 821
BsrBI CCGCTC 2 cut(s) 192, 451
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 81, 469
Bst6I CTCTTC 4 cut(s) 70, 388, 475, 546
BstC8I GCNNGC 4 cut(s) 298, 398, 449, 751
BstDEI CTNAG 1 cut(s) 642
BstF5I GGATG 2 cut(s) 402, 703
BstFNI CGCG 3 cut(s) 165, 396, 749
BstH2I RGCGCY 1 cut(s) 713
BstHHI GCGC 1 cut(s) 712
BstMAI GTCTC 1 cut(s) 477
BstMCI CGRYCG 1 cut(s) 747
BstMWI GCNNNNNNNGC 5 cut(s) 131, 351, 360, 419, 746
BstSFI CTRYAG 1 cut(s) 355
BstUI CGCG 3 cut(s) 165, 396, 749
BstV1I GCAGC 3 cut(s) 109, 594, 768
BstV2I GAAGAC 1 cut(s) 690
BstZI CGGCCG 1 cut(s) 744
BsuRI GGCC 6 cut(s) 140, 440, 454, 495, 746, 798
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 2 cut(s) 402, 703
Cac8I GCNNGC 4 cut(s) 298, 398, 449, 751
CciNI GCGGCCGC 1 cut(s) 744
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 6 cut(s) 160, 169, 430, 453, 493, 796
CseI GACGC 1 cut(s) 547
CviAII CATG 1 cut(s) 196
DdeI CTNAG 1 cut(s) 642
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 438, 744
EagI CGGCCG 1 cut(s) 744
Eam1104I CTCTTC 4 cut(s) 70, 388, 475, 546
EarI CTCTTC 4 cut(s) 70, 388, 475, 546
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 400
EclXI CGGCCG 1 cut(s) 744
Eco24I GRGCYC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 3 cut(s) 160, 169, 430
Eco52I CGGCCG 1 cut(s) 744
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 92
Eco88I CYCGRG 2 cut(s) 155, 468
EcoICRI GAGCTC 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 169
EcoRV GATATC 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 402
FaeI CATG 1 cut(s) 199
FaiI YATR 2 cut(s) 93, 197
FaqI GGGAC 2 cut(s) 392, 515
FatI CATG 1 cut(s) 195
FauI CCCGC 2 cut(s) 197, 250
FokI GGATG 2 cut(s) 389, 710
FriOI GRGCYC 1 cut(s) 402
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 711
GsuI CTGGAG 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 713
HaeIII GGCC 6 cut(s) 140, 440, 454, 495, 746, 798
HapII CCGG 2 cut(s) 575, 785
HgaI GACGC 1 cut(s) 547
HhaI GCGC 1 cut(s) 712
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HinfI GANTC 3 cut(s) 380, 503, 829
HpaII CCGG 2 cut(s) 575, 785
HphI GGTGA 1 cut(s) 564
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 85, 327, 834
Hpy188III TCNNGA 3 cut(s) 155, 500, 548
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 3 cut(s) 501, 563, 623
HpyAV CCTTC 5 cut(s) 49, 125, 433, 640, 656
HpyCH4IV ACGT 2 cut(s) 621, 789
HpyF10VI GCNNNNNNNGC 5 cut(s) 131, 351, 360, 419, 746
HpyF3I CTNAG 1 cut(s) 642
HpySE526I ACGT 2 cut(s) 621, 789
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 710
LmnI GCTCC 3 cut(s) 117, 197, 568
Lsp1109I GCAGC 3 cut(s) 109, 594, 768
LweI GCATC 1 cut(s) 643
MaeI CTAG 1 cut(s) 131
MaeII ACGT 2 cut(s) 621, 789
MbiI CCGCTC 2 cut(s) 192, 451
MhlI GDGCHC 1 cut(s) 402
MlsI TGGCCA 1 cut(s) 440
MluNI TGGCCA 1 cut(s) 440
MlyI GAGTC 3 cut(s) 374, 497, 838
Mox20I TGGCCA 1 cut(s) 440
MscI TGGCCA 1 cut(s) 440
MseI TTAA 1 cut(s) 804
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 2 cut(s) 575, 785
MvnI CGCG 3 cut(s) 165, 396, 749
MwoI GCNNNNNNNGC 5 cut(s) 131, 351, 360, 419, 746
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 1 cut(s) 821
NotI GCGGCCGC 1 cut(s) 744
PaeR7I CTCGAG 1 cut(s) 155
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 423, 627
PleI GAGTC 3 cut(s) 374, 497, 837
PpsI GAGTC 3 cut(s) 374, 497, 837
PpuMI RGGWCCY 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 821
PspPI GGNCC 6 cut(s) 160, 169, 430, 453, 493, 796
PspPPI RGGWCCY 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 122
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 1 cut(s) 804
Sau96I GGNCC 6 cut(s) 160, 169, 430, 453, 493, 796
SchI GAGTC 3 cut(s) 374, 497, 838
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 155
SinI GGWCC 3 cut(s) 160, 169, 430
SlaI CTCGAG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SspMI CTAG 1 cut(s) 131
SstI GAGCTC 1 cut(s) 402
TaiI ACGT 2 cut(s) 624, 792
TaqI TCGA 6 cut(s) 156, 333, 348, 417, 426, 618
TauI GCSGC 9 cut(s) 56, 248, 311, 314, 454, 715, 743, 746, 749
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TseI GCWGC 3 cut(s) 122, 607, 781
TspDTI ATGAA 2 cut(s) 17, 212
TspGWI ACGGA 3 cut(s) 91, 133, 619
VpaK11BI GGWCC 3 cut(s) 160, 169, 430
XcmI CCANNNNNNNNNTGG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 155
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.