FvH4_4g26641

Lipid phosphate phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004265
Physical Location & Seq
Reverse (-)
27958019 .. 27960711
2693 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26641.t1

Sequence Viewer

Length: 1008 bp
ATGGGTTGGTGGAATCTTGGTTCCCTTGTTTCTAGTTTCCGTAGTCTATTTCACTTCCAGGCGGTGCAGGGTGATCGACTGGGCACGTATACCATCAAGTCTCATGGAGTTGCGGTTGCCAAGAACCACAAGCATGACTGGCTCATACTTGTGCTTCTTGGTGTCATAGAGATTGTGTTGTATATCATTGAGCCTTTCCACCGTTTCGTGGGGAAGGATATGATGACTGATCTCAAGTACCCCTTGAAAGAGAACACTGTGCCTGCTTGGGCTGTTCCTGTGTATGCAGTATTGCTCCCCATTGCCATTTTCCTTTTGTTCTATGTGCGTCGGAAGGATGTGTATGATCTTCACCACAGCATTCTTGGCCTGTTATTTTCTGTGTTGGTTACTGTTGTCATTACTGAAGCAATTAAAAATGGAGTTGGGCGTCCTAGACCAGATTTCTTTTGGCGCTGCTTTCCTGATGGAAAAGACTCTTATGATGATACGTGGGGAGATGTGATGTGCCATGGTAAGGCCAGTGTCATGAAGGAAGGGTATAAGAGTTTCCCCAGTGGCCATGCTTCAGGGTCGTTTGCAGGCTTAGGCTTCCTCGCCTTATACTTGAGTGGAAAGATTAAAGTTTTTGATAGAAAAGGGCATGTTGCGAAACTATGCATTGTCGCCCTTCCTCTACTGGGTGCTTCTCTTGTTGCGGTGTCCAGAGTATCTGACTATTGGCACCATTGGCAAGATGTCTTTGCTGGAAGTCTGTTAGGATTTGTTGTGGCTGCATTTTGCTACCGGCAGTTCTTTCCTGCCCCTTATCATGATGAAGGTTGGGGTCCTTATGCATATTTCCGAGCATTAGAGGAGACACGTAGCAATTCAAGCGCGGCGACCACTTCCAATGCACTCAGTGTGCAGGTCATGGGGGTTCATAATGGGATTCAACATGTCCCTGCACAGAATGACACCAGTATTGCATTTACATCATATGCTTCGAATAACTACACAAGCCTGTAG

Protein Analysis

336

Amino Acids

37.38

Weight (kDa)

8.73

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 122 - 266 8.3e-33 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 898
AccB1I GGYRCC 1 cut(s) 723
AccI GTMKAC 1 cut(s) 89
AccII CGCG 1 cut(s) 878
AciI CCGC 4 cut(s) 62, 113, 698, 878
AcoI YGGCCR 1 cut(s) 559
AcuI CTGAAG 2 cut(s) 426, 552
AcyI GRCGYC 1 cut(s) 430
AdeI CACNNNGTG 1 cut(s) 902
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 3 cut(s) 208, 517, 680
AflIII ACRYGT 2 cut(s) 860, 937
AgsI TTSAA 3 cut(s) 247, 873, 935
AjnI CCWGG 1 cut(s) 57
AloI GAACNNNNNNTCC 1 cut(s) 36
Alw26I GTCTC 2 cut(s) 105, 851
AoxI GGCC 3 cut(s) 367, 519, 559
ApeKI GCWGC 2 cut(s) 456, 773
AspLEI GCGC 2 cut(s) 456, 878
AspS9I GGNCC 1 cut(s) 827
AsuHPI GGTGA 2 cut(s) 83, 344
AsuII TTCGAA 1 cut(s) 986
AvaII GGWCC 1 cut(s) 827
BaeGI GKGCMC 1 cut(s) 86
BalI TGGCCA 1 cut(s) 561
BanI GGYRCC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 443, 760
BccI CCATC 2 cut(s) 101, 461
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 2 cut(s) 105, 851
BfaI CTAG 2 cut(s) 33, 435
BfmI CTRYAG 1 cut(s) 1004
BfoI RGCGCY 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 898
BisI GCNGC 3 cut(s) 457, 774, 879
BlsI GCNGC 3 cut(s) 458, 775, 880
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 827
BmgT120I GGNCC 1 cut(s) 827
BmiI GGNNCC 3 cut(s) 22, 725, 828
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 3 cut(s) 89, 549, 689
BmuI ACTGGG 3 cut(s) 89, 549, 689
Bpu10I CCTNAGC 1 cut(s) 586
Bpu14I TTCGAA 1 cut(s) 986
BpuEI CTTGAG 2 cut(s) 218, 628
BsaAI YACGTR 3 cut(s) 87, 492, 863
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 517, 680
Bse118I RCCGGY 1 cut(s) 786
Bse1I ACTGG 6 cut(s) 84, 143, 522, 555, 684, 960
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 3 cut(s) 208, 517, 680
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 913
BseNI ACTGG 6 cut(s) 84, 143, 522, 555, 684, 960
BseRI GAGGAG 1 cut(s) 869
BseSI GKGCMC 1 cut(s) 86
BseXI GCAGC 2 cut(s) 443, 760
BsgI GTGCAG 3 cut(s) 86, 926, 930
Bsh1236I CGCG 1 cut(s) 878
BshFI GGCC 3 cut(s) 369, 521, 561
BshNI GGYRCC 1 cut(s) 723
BsiSI CCGG 1 cut(s) 787
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 3 cut(s) 208, 517, 680
BsmAI GTCTC 2 cut(s) 105, 851
BsmFI GGGAC 1 cut(s) 926
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 3 cut(s) 369, 521, 561
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 3 cut(s) 73, 229, 346
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 4 cut(s) 62, 113, 698, 878
BspANI GGCC 3 cut(s) 369, 521, 561
BspCNI CTCAG 1 cut(s) 912
BspFNI CGCG 1 cut(s) 878
BspHI TCATGA 2 cut(s) 528, 811
BspLI GGNNCC 3 cut(s) 22, 725, 828
BspMI ACCTGC 1 cut(s) 898
BspT104I TTCGAA 1 cut(s) 986
BspT107I GGYRCC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 786
BsrI ACTGG 6 cut(s) 84, 143, 522, 555, 684, 960
BssAI RCCGGY 1 cut(s) 786
BssECI CCNNGG 1 cut(s) 511
BssMI GATC 3 cut(s) 73, 229, 346
BssNAI GTATAC 1 cut(s) 90
BssNI GRCGYC 1 cut(s) 430
BssT1I CCWWGG 1 cut(s) 511
Bst1107I GTATAC 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 3 cut(s) 203, 259, 394
BstACI GRCGYC 1 cut(s) 430
BstBAI YACGTR 3 cut(s) 87, 492, 863
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 2 cut(s) 264, 583
BstDEI CTNAG 2 cut(s) 586, 899
BstDSI CCRYGG 1 cut(s) 511
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 878
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 2 cut(s) 456, 878
BstKTI GATC 3 cut(s) 76, 232, 349
BstMAI GTCTC 2 cut(s) 105, 851
BstMBI GATC 3 cut(s) 73, 229, 346
BstMWI GCNNNNNNNGC 4 cut(s) 139, 366, 730, 873
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 2 cut(s) 647, 941
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 1004
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 878
BstV1I GCAGC 2 cut(s) 443, 760
BstZ17I GTATAC 1 cut(s) 90
BsuRI GGCC 3 cut(s) 369, 521, 561
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 1 cut(s) 343
BtsIMutI CAGTG 4 cut(s) 255, 529, 562, 907
BveI ACCTGC 1 cut(s) 898
Cac8I GCNNGC 2 cut(s) 264, 583
CciI TCATGA 2 cut(s) 528, 811
CfoI GCGC 2 cut(s) 456, 878
Cfr10I RCCGGY 1 cut(s) 786
Cfr13I GGNCC 1 cut(s) 827
CseI GACGC 2 cut(s) 317, 419
Csp6I GTAC 1 cut(s) 238
CviAII CATG 9 cut(s) 104, 134, 512, 529, 563, 644, 812, 913, 938
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 2 cut(s) 586, 899
DpnI GATC 3 cut(s) 75, 231, 348
DpnII GATC 3 cut(s) 73, 229, 346
DraIII CACNNNGTG 1 cut(s) 902
EaeI YGGCCR 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 827
Eco57I CTGAAG 2 cut(s) 426, 552
EcoO109I RGGNCCY 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 511
EcoT22I ATGCAT 2 cut(s) 662, 838
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 9 cut(s) 107, 137, 515, 532, 566, 647, 815, 916, 941
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 1 cut(s) 926
FatI CATG 9 cut(s) 103, 133, 511, 528, 562, 643, 811, 912, 937
FauNDI CATATG 1 cut(s) 979
FblI GTMKAC 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 457, 774, 879
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 3 cut(s) 457, 774, 879
FspBI CTAG 2 cut(s) 33, 435
GlaI GCGC 2 cut(s) 455, 877
GluI GCNGC 3 cut(s) 457, 774, 879
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 3 cut(s) 369, 521, 561
HapII CCGG 1 cut(s) 787
HgaI GACGC 2 cut(s) 317, 419
HhaI GCGC 2 cut(s) 456, 878
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 9 cut(s) 107, 137, 515, 532, 566, 647, 815, 916, 941
Hin6I GCGC 2 cut(s) 454, 876
HinP1I GCGC 2 cut(s) 454, 876
HinfI GANTC 3 cut(s) 13, 476, 931
HpaII CCGG 1 cut(s) 787
HphI GGTGA 2 cut(s) 83, 344
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 3 cut(s) 333, 715, 845
Hpy188III TCNNGA 4 cut(s) 464, 529, 705, 812
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 6 cut(s) 208, 328, 526, 530, 680, 812
HpyCH4III ACNGT 3 cut(s) 203, 259, 394
HpyCH4IV ACGT 3 cut(s) 86, 491, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 366, 730, 873
HpyF3I CTNAG 2 cut(s) 586, 899
HpySE526I ACGT 3 cut(s) 86, 491, 862
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 9 cut(s) 107, 137, 515, 532, 566, 647, 815, 916, 941
HspAI GCGC 2 cut(s) 454, 876
Kzo9I GATC 3 cut(s) 73, 229, 346
LmnI GCTCC 1 cut(s) 300
Lsp1109I GCAGC 2 cut(s) 443, 760
MaeI CTAG 2 cut(s) 33, 435
MaeII ACGT 3 cut(s) 86, 491, 862
MaeIII GTNAC 1 cut(s) 388
MalI GATC 3 cut(s) 75, 231, 348
MboI GATC 3 cut(s) 73, 229, 346
MboII GAAGA 1 cut(s) 341
MhlI GDGCHC 1 cut(s) 86
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 2 cut(s) 411, 868
MluNI TGGCCA 1 cut(s) 561
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 3 cut(s) 605, 684, 847
Mox20I TGGCCA 1 cut(s) 561
Mph1103I ATGCAT 2 cut(s) 662, 838
MscI TGGCCA 1 cut(s) 561
MseI TTAA 2 cut(s) 414, 621
MslI CAYNNNNRTG 2 cut(s) 132, 149
Msp20I TGGCCA 1 cut(s) 561
MspI CCGG 1 cut(s) 787
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 360
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 1 cut(s) 878
MwoI GCNNNNNNNGC 4 cut(s) 139, 366, 730, 873
NcoI CCATGG 1 cut(s) 511
NdeI CATATG 1 cut(s) 979
NdeII GATC 3 cut(s) 73, 229, 346
NlaIII CATG 9 cut(s) 107, 137, 515, 532, 566, 647, 815, 916, 941
NlaIV GGNNCC 3 cut(s) 22, 725, 828
NsiI ATGCAT 2 cut(s) 662, 838
NspI RCATGY 2 cut(s) 647, 941
NspV TTCGAA 1 cut(s) 986
PagI TCATGA 2 cut(s) 528, 811
PciI ACATGT 1 cut(s) 937
PctI GAATGC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 13, 931
PkrI GCNGC 3 cut(s) 458, 775, 880
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
Ppu21I YACGTR 3 cut(s) 87, 492, 863
PpuMI RGGWCCY 1 cut(s) 827
PscI ACATGT 1 cut(s) 937
Psp5II RGGWCCY 1 cut(s) 827
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 3 cut(s) 22, 725, 828
PspPI GGNCC 1 cut(s) 827
PspPPI RGGWCCY 1 cut(s) 827
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 2 cut(s) 132, 149
SaqAI TTAA 2 cut(s) 414, 621
SatI GCNGC 3 cut(s) 457, 774, 879
Sau3AI GATC 3 cut(s) 73, 229, 346
Sau96I GGNCC 1 cut(s) 827
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 1 cut(s) 86
SetI ASST 5 cut(s) 89, 494, 823, 865, 912
SfcI CTRYAG 1 cut(s) 1004
SfuI TTCGAA 1 cut(s) 986
SinI GGWCC 1 cut(s) 827
SmiMI CAYNNNNRTG 2 cut(s) 132, 149
SmlI CTYRAG 2 cut(s) 233, 607
SmoI CTYRAG 2 cut(s) 233, 607
Sse9I AATT 2 cut(s) 411, 868
SsiI CCGC 4 cut(s) 62, 113, 698, 878
SspMI CTAG 2 cut(s) 33, 435
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 203, 259, 394
TaiI ACGT 3 cut(s) 89, 494, 865
TaqI TCGA 2 cut(s) 76, 986
TasI AATT 2 cut(s) 411, 868
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 2 cut(s) 13, 931
Tru1I TTAA 2 cut(s) 414, 621
Tru9I TTAA 2 cut(s) 414, 621
TscAI CASTG 4 cut(s) 262, 529, 562, 907
TseI GCWGC 2 cut(s) 456, 773
TspDTI ATGAA 3 cut(s) 545, 831, 911
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 4 cut(s) 262, 529, 562, 907
VpaK11BI GGWCC 1 cut(s) 827
XceI RCATGY 2 cut(s) 647, 941
XcmI CCANNNNNNNNNTGG 2 cut(s) 362, 447
XmiI GTMKAC 1 cut(s) 89
XspI CTAG 2 cut(s) 33, 435
Zsp2I ATGCAT 2 cut(s) 662, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.