Rh4CG354300

Lipid phosphate phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004265
Physical Location & Seq
Reverse (-)
61051800 .. 61054911
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG354300.1

Sequence Viewer

Length: 750 bp
ATGTATGCAGTCTTGGTTCCTATTACCATTTTTCTTTTCTTCTATGCGCGTAGGAAAGATGTGTATGATCTTCACCACAGCATTCTTGGCCTGTTATTTGCTGTGTTGGTTACTGCTGTCATTACTGATTCAATAAAAAATGGAGTTGGGCGTCCTAGACCAGATTTCTTTTGGCGTTGCTTTCCTGATGGAATCGATAATTATGATAAGTGGGGAGATGTGGTGTGCCATGGTAAGGCCAAGGACATAAAGGAAGGACATAAGAGTTTCCCGAGTGGCCATACTTCATGGTCCTTTGCAGGCTTAGGCTTCCTTGCCTTCTACTTGAGTGGAAAGATTAAAGTTTTTGATCGAAAAGGGCATGTTGCGAAACTATGCATTGTCTCCCTTCCTCTACTGGTTGCATCTCTCGTTGCAGTGTCCAGAGTATCTGACTATTGGCACCATTGGCAAGATGTGTTTGCTGGAGGTCTTTTAGGATTTGTTGTGGCTGCATTTTGCTACCGGCAGTTCTTCCCTGCCCCTTATCATGATCAAGGATGGGGTCCTTATGCATATTTCCAAGCATTGGAGGAGACGCGTAGCAATTCAAGCTCGGCGTCCACTGCAAATGCACTCAGTGTGCAGGTCATGGGGGTTCAGAACGTTACTCAACCTGTTCCTGCACAAAATGGTGTTGCATTTTCGTCATATGCTTCAAATCAATATACAAGCCTTAAGCTAGATGAAATGGAATCTGGGAGGAGGTGA

Protein Analysis

249

Amino Acids

27.77

Weight (kDa)

8.81

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 29 - 172 3.4e-32 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 616
AccB1I GGYRCC 1 cut(s) 441
AccB7I CCANNNNNTGG 1 cut(s) 568
AccII CGCG 2 cut(s) 49, 580
AclI AACGTT 1 cut(s) 645
AcoI YGGCCR 1 cut(s) 277
AcyI GRCGYC 2 cut(s) 151, 599
AdeI CACNNNGTG 1 cut(s) 620
AfiI CCNNNNNNNGG 2 cut(s) 235, 568
AflII CTTAAG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 578
AgsI TTSAA 3 cut(s) 132, 591, 699
AluBI AGCT 2 cut(s) 594, 721
AluI AGCT 2 cut(s) 594, 721
Alw26I GTCTC 2 cut(s) 388, 569
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 3 cut(s) 88, 237, 277
ApeKI GCWGC 1 cut(s) 491
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 291, 545
AsuHPI GGTGA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 271
AvaII GGWCC 2 cut(s) 291, 545
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 182, 534
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 2 cut(s) 388, 569
BfaI CTAG 2 cut(s) 156, 722
BfrI CTTAAG 1 cut(s) 716
BfuAI ACCTGC 1 cut(s) 616
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme18I GGWCC 2 cut(s) 291, 545
BmeT110I CYCGRG 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 291, 545
BmiI GGNNCC 3 cut(s) 18, 443, 546
BmsI GCATC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 304
BpuEI CTTGAG 1 cut(s) 346
Bsa29I ATCGAT 1 cut(s) 195
BsaHI GRCGYC 2 cut(s) 151, 599
BsaJI CCNNGG 2 cut(s) 229, 240
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 568
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 229, 240
BseGI GGATG 1 cut(s) 545
BseLI CCNNNNNNNGG 2 cut(s) 235, 568
BseMII CTCAG 1 cut(s) 631
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 1 cut(s) 478
BsgI GTGCAG 2 cut(s) 644, 648
Bsh1236I CGCG 2 cut(s) 49, 580
BshFI GGCC 3 cut(s) 90, 239, 279
BshNI GGYRCC 1 cut(s) 441
BshVI ATCGAT 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 1 cut(s) 505
BslI CCNNNNNNNGG 2 cut(s) 235, 568
BsmAI GTCTC 2 cut(s) 388, 569
BsmBI CGTCTC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 81
BsnI GGCC 3 cut(s) 90, 239, 279
BsoBI CYCGRG 1 cut(s) 271
Bsp143I GATC 3 cut(s) 67, 349, 532
Bsp19I CCATGG 1 cut(s) 229
BspANI GGCC 3 cut(s) 90, 239, 279
BspCNI CTCAG 1 cut(s) 630
BspDI ATCGAT 1 cut(s) 195
BspFNI CGCG 2 cut(s) 49, 580
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 18, 443, 546
BspMI ACCTGC 1 cut(s) 616
BspT107I GGYRCC 1 cut(s) 441
BspTI CTTAAG 1 cut(s) 716
BsrFI RCCGGY 1 cut(s) 504
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 229, 240
BssMI GATC 3 cut(s) 67, 349, 532
BssNI GRCGYC 2 cut(s) 151, 599
BssT1I CCWWGG 2 cut(s) 229, 240
BstACI GRCGYC 2 cut(s) 151, 599
BstAFI CTTAAG 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 2 cut(s) 304, 617
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 545
BstFNI CGCG 2 cut(s) 49, 580
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 3 cut(s) 70, 352, 535
BstMAI GTCTC 2 cut(s) 388, 569
BstMBI GATC 3 cut(s) 67, 349, 532
BstMWI GCNNNNNNNGC 4 cut(s) 87, 448, 591, 605
BstNSI RCATGY 1 cut(s) 365
BstUI CGCG 2 cut(s) 49, 580
BstV1I GCAGC 1 cut(s) 478
Bsu15I ATCGAT 1 cut(s) 195
BsuRI GGCC 3 cut(s) 90, 239, 279
BsuTUI ATCGAT 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 545
BtsI GCAGTG 2 cut(s) 423, 603
BtsIMutI CAGTG 3 cut(s) 423, 603, 625
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 1 cut(s) 301
CciI TCATGA 1 cut(s) 529
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 2 cut(s) 291, 545
ClaI ATCGAT 1 cut(s) 195
CseI GACGC 3 cut(s) 140, 586, 588
CviAII CATG 5 cut(s) 230, 288, 362, 530, 631
CviJI RGCY 9 cut(s) 90, 239, 279, 303, 309, 491, 594, 714, 721
CviKI_1 RGCY 9 cut(s) 90, 239, 279, 303, 309, 491, 594, 714, 721
DdeI CTNAG 2 cut(s) 304, 617
DpnI GATC 3 cut(s) 69, 351, 534
DpnII GATC 3 cut(s) 67, 349, 532
DraIII CACNNNGTG 1 cut(s) 620
EaeI YGGCCR 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 229, 240
Eco47I GGWCC 2 cut(s) 291, 545
Eco88I CYCGRG 1 cut(s) 271
EcoO109I RGGNCCY 1 cut(s) 545
EcoT14I CCWWGG 2 cut(s) 229, 240
EcoT22I ATGCAT 2 cut(s) 380, 556
ErhI CCWWGG 2 cut(s) 229, 240
Esp3I CGTCTC 1 cut(s) 569
FaeI CATG 5 cut(s) 233, 291, 365, 533, 634
FatI CATG 5 cut(s) 229, 287, 361, 529, 630
FauNDI CATATG 1 cut(s) 691
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 1 cut(s) 552
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 2 cut(s) 156, 722
GlaI GCGC 1 cut(s) 48
GluI GCNGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 3 cut(s) 90, 239, 279
HapII CCGG 1 cut(s) 505
HgaI GACGC 3 cut(s) 140, 586, 588
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 2 cut(s) 151, 599
Hin1II CATG 5 cut(s) 233, 291, 365, 533, 634
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 3 cut(s) 128, 192, 734
HpaII CCGG 1 cut(s) 505
HphI GGTGA 1 cut(s) 65
Hpy166II GTNNAC 1 cut(s) 603
Hpy188I TCNGA 2 cut(s) 433, 642
Hpy188III TCNNGA 4 cut(s) 185, 271, 423, 530
Hpy8I GTNNAC 1 cut(s) 603
HpyAV CCTTC 3 cut(s) 248, 328, 398
HpyCH4IV ACGT 1 cut(s) 645
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 448, 591, 605
HpyF3I CTNAG 2 cut(s) 304, 617
HpySE526I ACGT 1 cut(s) 645
Hsp92I GRCGYC 2 cut(s) 151, 599
Hsp92II CATG 5 cut(s) 233, 291, 365, 533, 634
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 3 cut(s) 67, 349, 532
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 413
MaeI CTAG 2 cut(s) 156, 722
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 2 cut(s) 109, 646
MalI GATC 3 cut(s) 69, 351, 534
MboI GATC 3 cut(s) 67, 349, 532
MboII GAAGA 3 cut(s) 31, 62, 505
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 2 cut(s) 199, 586
MluI ACGCGT 1 cut(s) 578
MluNI TGGCCA 1 cut(s) 279
MnlI CCTC 5 cut(s) 402, 461, 565, 735, 738
Mox20I TGGCCA 1 cut(s) 279
Mph1103I ATGCAT 2 cut(s) 380, 556
MscI TGGCCA 1 cut(s) 279
MseI TTAA 2 cut(s) 339, 717
Msp20I TGGCCA 1 cut(s) 279
MspCI CTTAAG 1 cut(s) 716
MspI CCGG 1 cut(s) 505
Mva1269I GAATGC 1 cut(s) 81
MvnI CGCG 2 cut(s) 49, 580
MwoI GCNNNNNNNGC 4 cut(s) 87, 448, 591, 605
NcoI CCATGG 1 cut(s) 229
NdeI CATATG 1 cut(s) 691
NdeII GATC 3 cut(s) 67, 349, 532
NlaIII CATG 5 cut(s) 233, 291, 365, 533, 634
NlaIV GGNNCC 3 cut(s) 18, 443, 546
NmeAIII GCCGAG 1 cut(s) 575
NsiI ATGCAT 2 cut(s) 380, 556
NspI RCATGY 1 cut(s) 365
PagI TCATGA 1 cut(s) 529
PctI GAATGC 1 cut(s) 81
PfeI GAWTC 3 cut(s) 128, 192, 734
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 1 cut(s) 493
PpuMI RGGWCCY 1 cut(s) 545
Psp1406I AACGTT 1 cut(s) 645
Psp5II RGGWCCY 1 cut(s) 545
PspN4I GGNNCC 3 cut(s) 18, 443, 546
PspPI GGNCC 2 cut(s) 291, 545
PspPPI RGGWCCY 1 cut(s) 545
SaqAI TTAA 2 cut(s) 339, 717
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 3 cut(s) 67, 349, 532
Sau96I GGNCC 2 cut(s) 291, 545
SetI ASST 7 cut(s) 472, 596, 630, 648, 658, 723, 749
SfaNI GCATC 1 cut(s) 413
SinI GGWCC 2 cut(s) 291, 545
SmlI CTYRAG 2 cut(s) 325, 716
SmoI CTYRAG 2 cut(s) 325, 716
Sse9I AATT 2 cut(s) 199, 586
SspMI CTAG 2 cut(s) 156, 722
StyI CCWWGG 2 cut(s) 229, 240
TaiI ACGT 1 cut(s) 648
TaqI TCGA 2 cut(s) 195, 352
TasI AATT 2 cut(s) 199, 586
TfiI GAWTC 3 cut(s) 128, 192, 734
Tru1I TTAA 2 cut(s) 339, 717
Tru9I TTAA 2 cut(s) 339, 717
TscAI CASTG 3 cut(s) 423, 610, 625
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 276, 741
TspRI CASTG 3 cut(s) 423, 610, 625
Van91I CCANNNNNTGG 1 cut(s) 568
Vha464I CTTAAG 1 cut(s) 716
VpaK11BI GGWCC 2 cut(s) 291, 545
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 2 cut(s) 83, 168
XspI CTAG 2 cut(s) 156, 722
Zsp2I ATGCAT 2 cut(s) 380, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.