FvH4_4g26974

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004921
Physical Location & Seq
Reverse (-)
28158105 .. 28159469
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26974.t1

Sequence Viewer

Length: 1230 bp
ATGAAGCAGGGTAAGCTTACTGTGATTGTAAAGGAGCTTTCCACAGGTTATGTTCATCCTAAAACCACCATCATACTCTCTTTGTGCGTGGCATTGTTTCTTTTCGTTCTTGGTATGTTCATTATCGTTCACACTACAAAGTTCTTAAGCTCAGAGTACTACTCCATCCCTCAGCTGAAGACATACTCCCCACTAAGTCCTAACTGCACTTGCCATGCACAAAGTCACTGCATCTTTTCTGTGTCTCCACAGTTACTCCCTATCAGTACTCTTCCAAATCTGAGTGATTACATAGCCCCCAAAGAGCTATGGCATTCCATGAGTGATCAGGAGCTGCTATGGCGAGCCTCTATGGCGCCGCAGGTCACCGAATACCCTTACAATCGAACACCAAAGGTTGCATTCATGTTCCTAACAAAGGGGAAACTTCCATTGGCGCCTCTTTGGGAAAGGTTTTTCAGGGGACATGAAGGCTTCTACTCAGTTTATCTTCATGCAGCACCAGACTTTAAGAACGAGCCACCCGAATCCTCAGTGTTCTACAAACGAATGATACCAAGCAAGGCAGTTGAATGGGGAAAGGTCACAATGGTTGAAGCAGAGAGGCGACTATTAGCAAATGCCTTGCTTGACTTCTCAAATGAAAGATTTGTACTGCTCTCTGAATCATGCATACCTCTTTTCAACTTCACCACAGTCTATGACTACCTCACCAACTCCAAATACAGTTTCATCAGCTCATTCGACGACCCGAGAGGCACCGGCCGGGGGCGCTACAACAGACGAATGTGGCCAACCGTAACATTGTCCGATTGGCGCAAGGGCTCACAATGGTTTGAGGTTCACAGAAACCTTGCCCTAAAGATAGTATCAGATGTAACCTACTTCCCTATATTCAGGGACCTCTGCCAACCTCCTTGCTACAACGATGAGCACTATTTGGCAACTCTGGTGACTAAAGTTGGCCCCGGCGTGAACTCAAATAGAAGCATTACCTGGGCTGATTGGTCCATAGGAGGCCCGCACCCTGCAACATATGAGAGGCGAGATGTGAATGAAATGTTTCTAAATCAGTTGAGACATGGGTTTAATTGTACCTACAATGGTGGCATTAGTAACATTTGCCACCTCTTTGCTAGGAAATTCCATCCTAGTGCACTTGAGCCTCTGCTAAAAATTGCTCCGGCTCTCTTCAAATTTAACACCGATAATACTACCACCAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

410

Amino Acids

46.97

Weight (kDa)

9.06

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 133 - 359 3.4e-78 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 352
AccB1I GGYRCC 3 cut(s) 355, 436, 758
AciI CCGC 2 cut(s) 359, 1022
AcoI YGGCCR 2 cut(s) 763, 791
AcsI RAATTY 2 cut(s) 1142, 1196
AcuI CTGAAG 1 cut(s) 197
AcyI GRCGYC 2 cut(s) 356, 437
AfaI GTAC 4 cut(s) 158, 268, 654, 1096
AfiI CCNNNNNNNGG 2 cut(s) 418, 768
AflII CTTAAG 1 cut(s) 145
AgsI TTSAA 4 cut(s) 572, 596, 685, 1195
AjnI CCWGG 1 cut(s) 995
AjuI GAANNNNNNNTTGG 4 cut(s) 416, 448, 713, 745
AluBI AGCT 7 cut(s) 16, 37, 150, 175, 307, 334, 738
AluI AGCT 7 cut(s) 16, 37, 150, 175, 307, 334, 738
Alw21I GWGCWC 2 cut(s) 936, 1159
Alw26I GTCTC 2 cut(s) 249, 1072
Alw44I GTGCAC 1 cut(s) 1155
AlwNI CAGNNNCTG 1 cut(s) 334
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 4 cut(s) 763, 791, 964, 1018
ApaLI GTGCAC 1 cut(s) 1155
ApeKI GCWGC 2 cut(s) 334, 497
ApoI RAATTY 2 cut(s) 1142, 1196
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 4 cut(s) 358, 439, 774, 819
AspS9I GGNCC 4 cut(s) 901, 965, 1008, 1019
AsuC2I CCSGG 2 cut(s) 767, 969
AsuHPI GGTGA 4 cut(s) 358, 682, 703, 964
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 2 cut(s) 901, 1008
BaeGI GKGCMC 1 cut(s) 1159
BalI TGGCCA 1 cut(s) 793
BanI GGYRCC 3 cut(s) 355, 436, 758
BanII GRGCYC 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 936, 1159
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 321, 509
BccI CCATC 3 cut(s) 77, 173, 1155
BciT130I CCWGG 1 cut(s) 997
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 2 cut(s) 767, 969
BcoDI GTCTC 2 cut(s) 249, 1072
BfaI CTAG 2 cut(s) 1137, 1152
BfoI RGCGCY 3 cut(s) 359, 440, 775
BfrI CTTAAG 1 cut(s) 145
BfuAI ACCTGC 1 cut(s) 352
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 3 cut(s) 335, 359, 498
BlsI GCNGC 3 cut(s) 336, 360, 499
BmcAI AGTACT 2 cut(s) 158, 268
Bme1390I CCNGG 3 cut(s) 767, 969, 997
Bme18I GGWCC 2 cut(s) 901, 1008
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 4 cut(s) 901, 965, 1008, 1019
BmiI GGNNCC 5 cut(s) 357, 438, 760, 902, 967
BmrFI CCNGG 3 cut(s) 767, 969, 997
BmsI GCATC 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 146, 178
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 2 cut(s) 767, 969
BsaHI GRCGYC 2 cut(s) 356, 437
BsaJI CCNNGG 3 cut(s) 766, 967, 996
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 768
Bse118I RCCGGY 1 cut(s) 761
Bse1I ACTGG 1 cut(s) 1221
BseBI CCWGG 1 cut(s) 997
BseDI CCNNGG 3 cut(s) 766, 967, 996
BseGI GGATG 3 cut(s) 55, 165, 1147
BseLI CCNNNNNNNGG 2 cut(s) 418, 768
BseMII CTCAG 5 cut(s) 165, 185, 272, 495, 546
BseNI ACTGG 1 cut(s) 1221
BseSI GKGCMC 1 cut(s) 1159
BseX3I CGGCCG 1 cut(s) 763
BseXI GCAGC 2 cut(s) 321, 509
BsgI GTGCAG 1 cut(s) 190
Bsh1285I CGRYCG 1 cut(s) 766
BshFI GGCC 4 cut(s) 765, 793, 966, 1020
BshNI GGYRCC 3 cut(s) 355, 436, 758
BsiEI CGRYCG 1 cut(s) 766
BsiHKAI GWGCWC 2 cut(s) 936, 1159
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 4 cut(s) 762, 766, 969, 1184
BslFI GGGAC 2 cut(s) 477, 914
BslI CCNNNNNNNGG 2 cut(s) 418, 768
BsmAI GTCTC 2 cut(s) 249, 1072
BsmFI GGGAC 2 cut(s) 477, 914
BsmI GAATGC 2 cut(s) 313, 401
BsnI GGCC 4 cut(s) 765, 793, 966, 1020
BsoBI CYCGRG 1 cut(s) 751
Bsp1286I GDGCHC 3 cut(s) 827, 936, 1159
Bsp143I GATC 1 cut(s) 325
BspACI CCGC 2 cut(s) 359, 1022
BspANI GGCC 4 cut(s) 765, 793, 966, 1020
BspCNI CTCAG 5 cut(s) 164, 184, 273, 494, 545
BspLI GGNNCC 5 cut(s) 357, 438, 760, 902, 967
BspMI ACCTGC 1 cut(s) 352
BspT107I GGYRCC 3 cut(s) 355, 436, 758
BspTI CTTAAG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 761
BsrI ACTGG 1 cut(s) 1221
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 766, 967, 996
BssMI GATC 1 cut(s) 325
BssNI GRCGYC 2 cut(s) 356, 437
Bst2UI CCWGG 1 cut(s) 997
Bst4CI ACNGT 5 cut(s) 22, 252, 697, 728, 799
Bst6I CTCTTC 2 cut(s) 276, 1196
BstACI GRCGYC 2 cut(s) 356, 437
BstAFI CTTAAG 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 345, 1022
BstDEI CTNAG 6 cut(s) 151, 171, 194, 281, 481, 532
BstEII GGTNACC 1 cut(s) 364
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 3 cut(s) 55, 165, 1147
BstH2I RGCGCY 3 cut(s) 359, 440, 775
BstHHI GCGC 4 cut(s) 358, 439, 774, 819
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 2 cut(s) 249, 1072
BstMBI GATC 1 cut(s) 325
BstMCI CGRYCG 1 cut(s) 766
BstMWI GCNNNNNNNGC 4 cut(s) 13, 340, 353, 771
BstNI CCWGG 1 cut(s) 997
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 3 cut(s) 765, 967, 995
BstSLI GKGCMC 1 cut(s) 1159
BstV1I GCAGC 2 cut(s) 321, 509
BstV2I GAAGAC 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 763
BsuRI GGCC 4 cut(s) 765, 793, 966, 1020
BtsCI GGATG 3 cut(s) 55, 165, 1147
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 226, 540
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 345, 1022
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 4 cut(s) 358, 439, 774, 819
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 4 cut(s) 901, 965, 1008, 1019
Csp6I GTAC 4 cut(s) 157, 267, 653, 1095
CviAII CATG 7 cut(s) 215, 319, 406, 467, 494, 669, 1082
CviQI GTAC 4 cut(s) 157, 267, 653, 1095
DdeI CTNAG 6 cut(s) 151, 171, 194, 281, 481, 532
DinI GGCGCC 2 cut(s) 357, 438
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 763, 791
EagI CGGCCG 1 cut(s) 763
Eam1104I CTCTTC 2 cut(s) 276, 1196
EarI CTCTTC 2 cut(s) 276, 1196
EclXI CGGCCG 1 cut(s) 763
Eco24I GRGCYC 1 cut(s) 827
Eco47I GGWCC 2 cut(s) 901, 1008
Eco52I CGGCCG 1 cut(s) 763
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 751
Eco91I GGTNACC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO109I RGGNCCY 1 cut(s) 901
EcoO65I GGTNACC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 995
EcoT22I ATGCAT 1 cut(s) 674
EcoT38I GRGCYC 1 cut(s) 827
EgeI GGCGCC 2 cut(s) 357, 438
EheI GGCGCC 2 cut(s) 357, 438
FaeI CATG 7 cut(s) 218, 322, 409, 470, 497, 672, 1085
FaqI GGGAC 2 cut(s) 477, 914
FatI CATG 7 cut(s) 214, 318, 405, 466, 493, 668, 1081
FauI CCCGC 1 cut(s) 1029
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 335, 359, 498
FokI GGATG 3 cut(s) 42, 152, 1134
FriOI GRGCYC 1 cut(s) 827
Fsp4HI GCNGC 3 cut(s) 335, 359, 498
FspBI CTAG 2 cut(s) 1137, 1152
GlaI GCGC 4 cut(s) 357, 438, 773, 818
GluI GCNGC 3 cut(s) 335, 359, 498
HaeII RGCGCY 3 cut(s) 359, 440, 775
HaeIII GGCC 4 cut(s) 765, 793, 966, 1020
HapII CCGG 4 cut(s) 762, 766, 969, 1184
HhaI GCGC 4 cut(s) 358, 439, 774, 819
Hin1I GRCGYC 2 cut(s) 356, 437
Hin1II CATG 7 cut(s) 218, 322, 409, 470, 497, 672, 1085
Hin6I GCGC 4 cut(s) 356, 437, 772, 817
HinP1I GCGC 4 cut(s) 356, 437, 772, 817
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 2 cut(s) 527, 665
HpaII CCGG 4 cut(s) 762, 766, 969, 1184
HphI GGTGA 4 cut(s) 358, 682, 703, 964
Hpy166II GTNNAC 4 cut(s) 130, 844, 976, 1157
Hpy188I TCNGA 5 cut(s) 154, 282, 664, 811, 874
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 4 cut(s) 130, 844, 976, 1157
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 5 cut(s) 22, 252, 697, 728, 799
HpyCH4V TGCA 8 cut(s) 207, 218, 231, 401, 497, 672, 1031, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 340, 353, 771
HpyF3I CTNAG 6 cut(s) 151, 171, 194, 281, 481, 532
Hsp92I GRCGYC 2 cut(s) 356, 437
Hsp92II CATG 7 cut(s) 218, 322, 409, 470, 497, 672, 1085
HspAI GCGC 4 cut(s) 356, 437, 772, 817
KasI GGCGCC 2 cut(s) 355, 436
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 3 cut(s) 34, 331, 1186
Lsp1109I GCAGC 2 cut(s) 321, 509
LweI GCATC 1 cut(s) 240
MaeI CTAG 2 cut(s) 1137, 1152
MaeIII GTNAC 8 cut(s) 224, 252, 364, 583, 799, 877, 952, 1115
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 4 cut(s) 190, 263, 482, 1183
MhlI GDGCHC 3 cut(s) 827, 936, 1159
MlsI TGGCCA 1 cut(s) 793
MluCI AATT 4 cut(s) 1090, 1142, 1176, 1196
MluNI TGGCCA 1 cut(s) 793
Mly113I GGCGCC 2 cut(s) 356, 437
Mox20I TGGCCA 1 cut(s) 793
Mph1103I ATGCAT 1 cut(s) 674
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 793
MseI TTAA 4 cut(s) 146, 510, 1089, 1200
MslI CAYNNNNRTG 1 cut(s) 1152
Msp20I TGGCCA 1 cut(s) 793
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 4 cut(s) 762, 766, 969, 1184
MspR9I CCNGG 3 cut(s) 767, 969, 997
Mva1269I GAATGC 2 cut(s) 313, 401
MvaI CCWGG 1 cut(s) 997
MwoI GCNNNNNNNGC 4 cut(s) 13, 340, 353, 771
NarI GGCGCC 2 cut(s) 356, 437
NciI CCSGG 2 cut(s) 767, 969
NdeI CATATG 1 cut(s) 1036
NdeII GATC 1 cut(s) 325
NlaIII CATG 7 cut(s) 218, 322, 409, 470, 497, 672, 1085
NlaIV GGNNCC 5 cut(s) 357, 438, 760, 902, 967
NmuCI GTSAC 4 cut(s) 224, 364, 583, 952
NsiI ATGCAT 1 cut(s) 674
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 2 cut(s) 313, 401
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 2 cut(s) 527, 665
PkrI GCNGC 3 cut(s) 336, 360, 499
PluTI GGCGCC 2 cut(s) 359, 440
PpuMI RGGWCCY 1 cut(s) 901
Psp5II RGGWCCY 1 cut(s) 901
Psp6I CCWGG 1 cut(s) 995
PspEI GGTNACC 1 cut(s) 364
PspGI CCWGG 1 cut(s) 995
PspN4I GGNNCC 5 cut(s) 357, 438, 760, 902, 967
PspPI GGNCC 4 cut(s) 901, 965, 1008, 1019
PspPPI RGGWCCY 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 4 cut(s) 158, 268, 654, 1096
RsaNI GTAC 4 cut(s) 157, 267, 653, 1095
RseI CAYNNNNRTG 1 cut(s) 1152
SaqAI TTAA 4 cut(s) 146, 510, 1089, 1200
SatI GCNGC 3 cut(s) 335, 359, 498
Sau3AI GATC 1 cut(s) 325
Sau96I GGNCC 4 cut(s) 901, 965, 1008, 1019
ScaI AGTACT 2 cut(s) 158, 268
ScrFI CCNGG 3 cut(s) 767, 969, 997
SduI GDGCHC 3 cut(s) 827, 936, 1159
SfaNI GCATC 1 cut(s) 240
SfoI GGCGCC 2 cut(s) 357, 438
SinI GGWCC 2 cut(s) 901, 1008
SmiMI CAYNNNNRTG 1 cut(s) 1152
SmlI CTYRAG 2 cut(s) 145, 1160
SmoI CTYRAG 2 cut(s) 145, 1160
Sse9I AATT 4 cut(s) 1090, 1142, 1176, 1196
SsiI CCGC 2 cut(s) 359, 1022
SspDI GGCGCC 2 cut(s) 355, 436
SspMI CTAG 2 cut(s) 1137, 1152
StyD4I CCNGG 3 cut(s) 765, 967, 995
TaaI ACNGT 5 cut(s) 22, 252, 697, 728, 799
TaqI TCGA 2 cut(s) 385, 744
TasI AATT 4 cut(s) 1090, 1142, 1176, 1196
TatI WGTACW 3 cut(s) 156, 266, 652
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 2 cut(s) 527, 665
Tru1I TTAA 4 cut(s) 146, 510, 1089, 1200
Tru9I TTAA 4 cut(s) 146, 510, 1089, 1200
TscAI CASTG 2 cut(s) 233, 540
TseFI GTSAC 4 cut(s) 224, 364, 583, 952
TseI GCWGC 2 cut(s) 334, 497
Tsp45I GTSAC 4 cut(s) 224, 364, 583, 952
TspDTI ATGAA 9 cut(s) 17, 44, 109, 394, 482, 483, 657, 721, 1071
TspRI CASTG 2 cut(s) 233, 540
Vha464I CTTAAG 1 cut(s) 145
VneI GTGCAC 1 cut(s) 1155
VpaK11BI GGWCC 2 cut(s) 901, 1008
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 2 cut(s) 1142, 1196
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 2 cut(s) 1137, 1152
ZrmI AGTACT 2 cut(s) 158, 268
Zsp2I ATGCAT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.