Rroxscaffold_5G00375970

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004921
Physical Location & Seq
Reverse (-)
56749557 .. 56750866
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375970.1

Sequence Viewer

Length: 1224 bp
ATGAAGCAGGGTAAGCTCACAGTGTTTGTAAAGGACCTTTCCACAGGCTATTTGCATGTTAAAACCACTACCATACTCTCTTTGTCTGTATTATTGCTTCTTTTCGTTCTGGGTATGTTCATTATCGATCACACTACGAAAGTCTTAAGTTCAGAGGTCTACTTCATCCCCCAGCTGAAGACATTTTCCCCACTCACAGCTAATTGCACTAGCCACGCACAATGTCAATGCAACTTTTCTGCGTCTCCGCAGTTACTCCCTATTAGTTCTCTTCCAAATATAAGAGATTACATAGCTCCCAAAGAGCTATGGCACTCCATGAGTGATAAGGAGCTAATATGGCGAGCCTCTATGGTGCCACAGGTCACCGAATACCCTTATAATCGAACGCCAAAGGTCGCATTCATGTTCCTTGCAAAGGGAAAACTGCCATTGGCGCCTCTTTGGGAAAGGTTTTTCATGGGACATGAAGGCTTCTTCTCAGTTTATCTCCATGCAGCACCGGACTTTAAGAACGAGCCCCCGAATCCTCAGTGTTTTACAATCGGAGGATACCAAGCAGTTGAATGGGGAAAGGCCACAATGGTTGAAGCAGAGAGGCGCCTATTAGCCAATGCCTTACTTGACTTCTCAAATGAGAGATTTGTACTGCTCTCTGAATCATGCATACCTCTCTTCAACTTCACCACAATCTATGACTACCTCATCAACTCCAACTACAGTTTCATTAGCTCATTCGATGACCCGAGAGGCACCGGCCGGGGGCGCTACAACAGGCGAATGTGGCCAACCGTGACATTGTCCGATTGGCGCAAGGGTTCACAATGGTTCGAAGTTCACAGAAACCTTGCCCTGAAGATAGTATCAGATGTAACCTACTACCCTATATTTAAGGACCTCTGCCAGCCTCCTTGCTACAACGATGAACACTATTTGGCAACCCTGGTGAGCAAAGTTGGCACCGGCATGAACTCAAATAGGAGCATTACCTGGGTTGATTGGTCCGTAGGTGGCCCGCACCCTGCAAAATATGGGAGGCAACATGTGACAGAAGCGTTTCTGAATCAGTTGAGACATGGGTTTGATTGTACCTACAATGGTGGCGTTAGTAACATTTGCCACGTCTTTGCTAGGAAATTCCATCCTAGTACACTTGAGCCTCTGCTAAAAATAGCTCCGACTCTGTTTGGATTTAATACCGATAATATTACCACTACAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

407

Amino Acids

46.42

Weight (kDa)

8.83

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 133 - 358 1.8e-72 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AccB1I GGYRCC 5 cut(s) 355, 436, 600, 752, 959
AccI GTMKAC 1 cut(s) 159
AciI CCGC 2 cut(s) 248, 1016
AcoI YGGCCR 2 cut(s) 757, 785
AcsI RAATTY 1 cut(s) 1136
AcuI CTGAAG 2 cut(s) 197, 875
AcyI GRCGYC 2 cut(s) 437, 601
AfaI GTAC 3 cut(s) 648, 1090, 1150
AfiI CCNNNNNNNGG 2 cut(s) 418, 762
AflII CTTAAG 1 cut(s) 145
AflIII ACRYGT 1 cut(s) 1042
AgsI TTSAA 3 cut(s) 566, 590, 679
AjiI CACGTC 1 cut(s) 1123
AjnI CCWGG 2 cut(s) 942, 989
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
AluBI AGCT 8 cut(s) 16, 175, 200, 296, 307, 334, 732, 1175
AluI AGCT 8 cut(s) 16, 175, 200, 296, 307, 334, 732, 1175
Alw26I GTCTC 2 cut(s) 249, 1066
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 4 cut(s) 576, 757, 785, 1012
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 1 cut(s) 1136
Asp700I GAANNNNTTC 1 cut(s) 1056
AspLEI GCGC 4 cut(s) 439, 603, 768, 813
AspS9I GGNCC 4 cut(s) 34, 895, 1002, 1013
AsuC2I CCSGG 1 cut(s) 761
AsuHPI GGTGA 3 cut(s) 358, 676, 958
AsuII TTCGAA 1 cut(s) 831
AvaI CYCGRG 1 cut(s) 745
AvaII GGWCC 3 cut(s) 34, 895, 1002
BalI TGGCCA 1 cut(s) 787
BanI GGYRCC 5 cut(s) 355, 436, 600, 752, 959
BanII GRGCYC 1 cut(s) 522
BarI GAAGNNNNNNTAC 2 cut(s) 81, 113
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 509
BccI CCATC 1 cut(s) 1149
BciT130I CCWGG 2 cut(s) 944, 991
BciVI GTATCC 1 cut(s) 545
BcnI CCSGG 1 cut(s) 761
BcoDI GTCTC 2 cut(s) 249, 1066
BfaI CTAG 3 cut(s) 210, 1131, 1146
BfmI CTRYAG 1 cut(s) 718
BfoI RGCGCY 3 cut(s) 440, 604, 769
BfrI CTTAAG 1 cut(s) 145
BfuI GTATCC 1 cut(s) 545
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
Bme1390I CCNGG 3 cut(s) 761, 944, 991
Bme18I GGWCC 3 cut(s) 34, 895, 1002
BmeT110I CYCGRG 1 cut(s) 745
BmgBI CACGTC 1 cut(s) 1123
BmgT120I GGNCC 4 cut(s) 34, 895, 1002, 1013
BmiI GGNNCC 5 cut(s) 357, 438, 602, 754, 961
BmrFI CCNGG 3 cut(s) 761, 944, 991
BpiI GAAGAC 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 1175
BpuMI CCSGG 1 cut(s) 761
Bsa29I ATCGAT 1 cut(s) 126
BsaHI GRCGYC 2 cut(s) 437, 601
BsaJI CCNNGG 3 cut(s) 760, 942, 990
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 762
Bse118I RCCGGY 2 cut(s) 755, 962
BseBI CCWGG 2 cut(s) 944, 991
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 760, 942, 990
BseGI GGATG 2 cut(s) 165, 1141
BseLI CCNNNNNNNGG 2 cut(s) 418, 762
BseMII CTCAG 2 cut(s) 495, 545
BseX3I CGGCCG 1 cut(s) 757
BseXI GCAGC 1 cut(s) 509
BseYI CCCAGC 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 4 cut(s) 578, 759, 787, 1014
BshNI GGYRCC 5 cut(s) 355, 436, 600, 752, 959
BshVI ATCGAT 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 760
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 4 cut(s) 503, 756, 760, 963
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 2 cut(s) 418, 762
BsmAI GTCTC 2 cut(s) 249, 1066
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 477
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 4 cut(s) 578, 759, 787, 1014
BsoBI CYCGRG 1 cut(s) 745
Bsp119I TTCGAA 1 cut(s) 831
Bsp1286I GDGCHC 1 cut(s) 522
Bsp143I GATC 1 cut(s) 127
BspACI CCGC 2 cut(s) 248, 1016
BspANI GGCC 4 cut(s) 578, 759, 787, 1014
BspCNI CTCAG 2 cut(s) 494, 544
BspDI ATCGAT 1 cut(s) 126
BspLI GGNNCC 5 cut(s) 357, 438, 602, 754, 961
BspT104I TTCGAA 1 cut(s) 831
BspT107I GGYRCC 5 cut(s) 355, 436, 600, 752, 959
BspTI CTTAAG 1 cut(s) 145
BsrFI RCCGGY 2 cut(s) 755, 962
BssAI RCCGGY 2 cut(s) 755, 962
BssECI CCNNGG 3 cut(s) 760, 942, 990
BssMI GATC 1 cut(s) 127
BssNI GRCGYC 2 cut(s) 437, 601
Bst2UI CCWGG 2 cut(s) 944, 991
Bst4CI ACNGT 3 cut(s) 22, 722, 793
Bst6I CTCTTC 2 cut(s) 276, 680
BstACI GRCGYC 2 cut(s) 437, 601
BstAFI CTTAAG 1 cut(s) 145
BstBI TTCGAA 1 cut(s) 831
BstC8I GCNNGC 3 cut(s) 345, 905, 1016
BstDEI CTNAG 2 cut(s) 481, 531
BstEII GGTNACC 1 cut(s) 364
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 2 cut(s) 165, 1141
BstH2I RGCGCY 3 cut(s) 440, 604, 769
BstHHI GCGC 4 cut(s) 439, 603, 768, 813
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 2 cut(s) 249, 1066
BstMBI GATC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 6 cut(s) 13, 340, 436, 765, 784, 957
BstNI CCWGG 2 cut(s) 944, 991
BstNSI RCATGY 2 cut(s) 59, 1046
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 3 cut(s) 759, 942, 989
BstSFI CTRYAG 1 cut(s) 718
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 757
Bsu15I ATCGAT 1 cut(s) 126
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 4 cut(s) 578, 759, 787, 1014
BsuTUI ATCGAT 1 cut(s) 126
BtrI CACGTC 1 cut(s) 1123
BtsCI GGATG 2 cut(s) 165, 1141
BtsIMutI CAGTG 2 cut(s) 27, 539
Cac8I GCNNGC 3 cut(s) 345, 905, 1016
CfoI GCGC 4 cut(s) 439, 603, 768, 813
Cfr10I RCCGGY 2 cut(s) 755, 962
Cfr13I GGNCC 4 cut(s) 34, 895, 1002, 1013
ClaI ATCGAT 1 cut(s) 126
CseI GACGC 1 cut(s) 231
Csp6I GTAC 3 cut(s) 647, 1089, 1149
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviQI GTAC 3 cut(s) 647, 1089, 1149
DdeI CTNAG 2 cut(s) 481, 531
DinI GGCGCC 2 cut(s) 438, 602
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 757, 785
EagI CGGCCG 1 cut(s) 757
Eam1104I CTCTTC 2 cut(s) 276, 680
EarI CTCTTC 2 cut(s) 276, 680
EclXI CGGCCG 1 cut(s) 757
Eco24I GRGCYC 1 cut(s) 522
Eco47I GGWCC 3 cut(s) 34, 895, 1002
Eco52I CGGCCG 1 cut(s) 757
Eco57I CTGAAG 2 cut(s) 197, 875
Eco88I CYCGRG 1 cut(s) 745
Eco91I GGTNACC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO109I RGGNCCY 2 cut(s) 34, 895
EcoO65I GGTNACC 1 cut(s) 364
EcoRII CCWGG 2 cut(s) 942, 989
EcoT22I ATGCAT 1 cut(s) 668
EcoT38I GRGCYC 1 cut(s) 522
EgeI GGCGCC 2 cut(s) 438, 602
EheI GGCGCC 2 cut(s) 438, 602
Esp3I CGTCTC 1 cut(s) 249
FaqI GGGAC 1 cut(s) 477
FauI CCCGC 1 cut(s) 1023
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 2 cut(s) 152, 1128
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 3 cut(s) 210, 1131, 1146
GlaI GCGC 4 cut(s) 438, 602, 767, 812
GluI GCNGC 1 cut(s) 498
GsaI CCCAGC 1 cut(s) 175
HaeII RGCGCY 3 cut(s) 440, 604, 769
HaeIII GGCC 4 cut(s) 578, 759, 787, 1014
HapII CCGG 4 cut(s) 503, 756, 760, 963
HgaI GACGC 1 cut(s) 231
HhaI GCGC 4 cut(s) 439, 603, 768, 813
Hin1I GRCGYC 2 cut(s) 437, 601
Hin6I GCGC 4 cut(s) 437, 601, 766, 811
HinP1I GCGC 4 cut(s) 437, 601, 766, 811
HinfI GANTC 4 cut(s) 526, 659, 1063, 1180
HpaII CCGG 4 cut(s) 503, 756, 760, 963
HphI GGTGA 3 cut(s) 358, 676, 958
Hpy166II GTNNAC 4 cut(s) 160, 821, 838, 1151
Hpy188I TCNGA 7 cut(s) 154, 548, 658, 805, 868, 1062, 1179
Hpy8I GTNNAC 4 cut(s) 160, 821, 838, 1151
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 3 cut(s) 22, 722, 793
HpyCH4IV ACGT 1 cut(s) 1122
HpyCH4V TGCA 7 cut(s) 55, 207, 231, 416, 497, 666, 1025
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 340, 436, 765, 784, 957
HpyF3I CTNAG 2 cut(s) 481, 531
HpySE526I ACGT 1 cut(s) 1122
Hsp92I GRCGYC 2 cut(s) 437, 601
HspAI GCGC 4 cut(s) 437, 601, 766, 811
KasI GGCGCC 2 cut(s) 436, 600
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 4 cut(s) 301, 331, 981, 1180
Lsp1109I GCAGC 1 cut(s) 509
MaeI CTAG 3 cut(s) 210, 1131, 1146
MaeII ACGT 1 cut(s) 1122
MaeIII GTNAC 6 cut(s) 252, 364, 793, 871, 1045, 1109
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 5 cut(s) 190, 263, 469, 667, 868
MhlI GDGCHC 1 cut(s) 522
MlsI TGGCCA 1 cut(s) 787
MluCI AATT 2 cut(s) 202, 1136
MluNI TGGCCA 1 cut(s) 787
Mly113I GGCGCC 2 cut(s) 437, 601
MlyI GAGTC 1 cut(s) 1174
MmeI TCCRAC 2 cut(s) 738, 1202
Mox20I TGGCCA 1 cut(s) 787
Mph1103I ATGCAT 1 cut(s) 668
MroXI GAANNNNTTC 1 cut(s) 1056
MscI TGGCCA 1 cut(s) 787
MseI TTAA 5 cut(s) 60, 146, 510, 891, 1194
MslI CAYNNNNRTG 1 cut(s) 965
Msp20I TGGCCA 1 cut(s) 787
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 4 cut(s) 503, 756, 760, 963
MspR9I CCNGG 3 cut(s) 761, 944, 991
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 2 cut(s) 944, 991
MwoI GCNNNNNNNGC 6 cut(s) 13, 340, 436, 765, 784, 957
NarI GGCGCC 2 cut(s) 437, 601
NciI CCSGG 1 cut(s) 761
NdeII GATC 1 cut(s) 127
NlaIV GGNNCC 5 cut(s) 357, 438, 602, 754, 961
NmuCI GTSAC 3 cut(s) 364, 793, 1045
NsiI ATGCAT 1 cut(s) 668
NspI RCATGY 2 cut(s) 59, 1046
NspV TTCGAA 1 cut(s) 831
PciI ACATGT 1 cut(s) 1042
PctI GAATGC 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 3 cut(s) 526, 659, 1063
PflFI GACNNNGTC 1 cut(s) 799
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 1 cut(s) 1174
PluTI GGCGCC 2 cut(s) 440, 604
PpsI GAGTC 1 cut(s) 1174
PpuMI RGGWCCY 2 cut(s) 34, 895
PscI ACATGT 1 cut(s) 1042
PsiI TTATAA 1 cut(s) 381
Psp5II RGGWCCY 2 cut(s) 34, 895
Psp6I CCWGG 2 cut(s) 942, 989
PspEI GGTNACC 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 2 cut(s) 942, 989
PspN4I GGNNCC 5 cut(s) 357, 438, 602, 754, 961
PspPI GGNCC 4 cut(s) 34, 895, 1002, 1013
PspPPI RGGWCCY 2 cut(s) 34, 895
PsyI GACNNNGTC 1 cut(s) 799
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 3 cut(s) 648, 1090, 1150
RsaNI GTAC 3 cut(s) 647, 1089, 1149
RseI CAYNNNNRTG 1 cut(s) 965
SaqAI TTAA 5 cut(s) 60, 146, 510, 891, 1194
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 4 cut(s) 34, 895, 1002, 1013
SchI GAGTC 1 cut(s) 1174
ScrFI CCNGG 3 cut(s) 761, 944, 991
SduI GDGCHC 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 718
SfoI GGCGCC 2 cut(s) 438, 602
SfuI TTCGAA 1 cut(s) 831
SinI GGWCC 3 cut(s) 34, 895, 1002
SmiMI CAYNNNNRTG 1 cut(s) 965
SmlI CTYRAG 2 cut(s) 145, 1154
SmoI CTYRAG 2 cut(s) 145, 1154
Sse9I AATT 2 cut(s) 202, 1136
SsiI CCGC 2 cut(s) 248, 1016
SspDI GGCGCC 2 cut(s) 436, 600
SspI AATATT 1 cut(s) 1207
SspMI CTAG 3 cut(s) 210, 1131, 1146
StyD4I CCNGG 3 cut(s) 759, 942, 989
TaaI ACNGT 3 cut(s) 22, 722, 793
TaiI ACGT 1 cut(s) 1125
TaqI TCGA 4 cut(s) 126, 385, 738, 831
TasI AATT 2 cut(s) 202, 1136
TatI WGTACW 2 cut(s) 646, 1148
TfiI GAWTC 3 cut(s) 526, 659, 1063
Tru1I TTAA 5 cut(s) 60, 146, 510, 891, 1194
Tru9I TTAA 5 cut(s) 60, 146, 510, 891, 1194
TscAI CASTG 2 cut(s) 27, 539
TseFI GTSAC 3 cut(s) 364, 793, 1045
TseI GCWGC 1 cut(s) 497
Tsp45I GTSAC 3 cut(s) 364, 793, 1045
TspDTI ATGAA 9 cut(s) 17, 109, 154, 394, 448, 483, 715, 939, 983
TspGWI ACGGA 1 cut(s) 994
TspRI CASTG 2 cut(s) 27, 539
Tth111I GACNNNGTC 1 cut(s) 799
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 3 cut(s) 34, 895, 1002
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 1 cut(s) 1136
XceI RCATGY 2 cut(s) 59, 1046
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 3 cut(s) 210, 1131, 1146
Zsp2I ATGCAT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.