MD14G1228300.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004921
Physical Location & Seq
Forward (+)
30928624 .. 30930025
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1228300.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGGAAGCGAACTCCGAAAATCTCAGCCAATTTCGAAGCTTTTGGTATCGTATTTGCAGTTTAGCCATGCCTTTCATCTAGTGTTTTTTGTCATTGGTTTGTCCATTGGAGTCATGGTCAGTTCATACTACAAGACCTTCTTCCCATTCACCTTACAAACCACTTCCTCCTCGTCACTGCTACTGCCGCCTGCCTCAATATCACAGTTTCTGCCACAACCACCCTCACCGCCGCTTCTCCCTTTACAGGAATCAGGTACTGATAACGTTGCATCGGATACTGAGGCACTTTTGGTGCACAAGGGGCCCATACCTTTGGCCCCATTGTGGGAAATGTTCTTCAATGGCCATGAAGGACTTTACAACATATATGTCCACCCTCATCCTTCATATGTTGAGTCATGGCCTCAAAATTCTGTTTTCTATGGAAGGAGAATTCCAAGCAAGGCGGTTGAGTGGGGAAGTCCTACAATGATCGATGGTGAGAGACGCCTGCTAGCAAGTGCCCTCCTTGACTTCACTAACGAGAGATTTGTGCTACTATCCGAGTCTTGCATCCCATTGTTCAACTTCACCACAATATACAGCTACCTCATAAATGCCAGTGAGAGCAATATAGGTTCATACGATGACCCGAGGAAGGTGGGTCGGGGCAGATACAATCCGAGAATGTGGCCTGAAATCAACATATCGGATTGGCGCAAAGGCTCCCAATGGTTCGAGGTCAGCCGGAAGCTGGCAGTTGAGATCATATCAGACAACAAGTACTACCCCATATTCAAAGAGCATTGCAGCCCTCCTTGCTACATGGATGAGCATTACATCCCAACCCTAGTGAACATCATTGGCCCTGAAGAAAACTCGAATCGGAGCGTTACTTGGGTCGATTGGTCCAAAAGTGGTCCTCACCCCGGAAGATTTGGGAGGAATGATGTTTCAGAAGTAATTTTGAATCAGATAAGATTTGGTTCTAATTGCAGTTGTAGTGGTAATAGTAGTGCAAATAGTACTACTACTTGTTCTATGTGTTTTTTGTTCGCTAGGAAGTTCATGGGAGACACTTTGCGGCCTCTCTTGCAAATTGCACCCATGTTGCTAGGTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

369

Amino Acids

41.52

Weight (kDa)

6.75

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 97 - 320 8.2e-77 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 188, 230, 233, 449, 1066
AclI AACGTT 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 2 cut(s) 412, 435
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 490
AfaI GTAC 3 cut(s) 259, 767, 1009
AfiI CCNNNNNNNGG 6 cut(s) 247, 327, 328, 640, 736, 911
AgsI TTSAA 4 cut(s) 343, 568, 781, 952
AluBI AGCT 3 cut(s) 40, 588, 736
AluI AGCT 3 cut(s) 40, 588, 736
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 2 cut(s) 481, 1050
Alw44I GTGCAC 1 cut(s) 296
AlwNI CAGNNNCTG 2 cut(s) 211, 260
Ama87I CYCGRG 1 cut(s) 634
AoxI GGCC 7 cut(s) 305, 318, 346, 404, 674, 847, 1067
ApaI GGGCCC 1 cut(s) 309
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 792
ApoI RAATTY 2 cut(s) 412, 435
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 6 cut(s) 305, 306, 319, 848, 891, 902
AsuC2I CCSGG 1 cut(s) 912
AsuHPI GGTGA 5 cut(s) 142, 219, 494, 565, 899
AsuII TTCGAA 1 cut(s) 35
AsuNHI GCTAGC 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 2 cut(s) 891, 902
BaeGI GKGCMC 3 cut(s) 300, 309, 508
BalI TGGCCA 1 cut(s) 348
BanII GRGCYC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 804
BccI CCATC 1 cut(s) 473
BciVI GTATCC 1 cut(s) 271
BcnI CCSGG 1 cut(s) 912
BcoDI GTCTC 2 cut(s) 481, 1050
BfaI CTAG 6 cut(s) 80, 497, 833, 1041, 1097, 1105
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 4 cut(s) 188, 233, 793, 1067
BlsI GCNGC 4 cut(s) 189, 234, 794, 1068
BmcAI AGTACT 2 cut(s) 767, 1009
Bme1390I CCNGG 1 cut(s) 912
Bme18I GGWCC 2 cut(s) 891, 902
BmeT110I CYCGRG 1 cut(s) 634
BmgT120I GGNCC 6 cut(s) 305, 306, 319, 848, 891, 902
BmiI GGNNCC 5 cut(s) 306, 307, 321, 709, 1102
BmrFI CCNGG 1 cut(s) 912
BmsI GCATC 2 cut(s) 281, 564
BmtI GCTAGC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 912
Bsa29I ATCGAT 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 635, 910
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 6 cut(s) 247, 327, 328, 640, 736, 911
Bse1I ACTGG 1 cut(s) 603
Bse3DI GCAATG 1 cut(s) 787
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 635, 910
BseGI GGATG 4 cut(s) 382, 555, 817, 822
BseLI CCNNNNNNNGG 6 cut(s) 247, 327, 328, 640, 736, 911
BseMI GCAATG 1 cut(s) 787
BseMII CTCAG 2 cut(s) 38, 273
BseNI ACTGG 1 cut(s) 603
BseRI GAGGAG 1 cut(s) 160
BseSI GKGCMC 3 cut(s) 300, 309, 508
BseXI GCAGC 1 cut(s) 804
BshFI GGCC 7 cut(s) 307, 320, 348, 406, 676, 849, 1069
BshVI ATCGAT 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 634
BsiSI CCGG 2 cut(s) 730, 912
BslI CCNNNNNNNGG 6 cut(s) 247, 327, 328, 640, 736, 911
BsmAI GTCTC 2 cut(s) 481, 1050
BsmBI CGTCTC 1 cut(s) 481
BsnI GGCC 7 cut(s) 307, 320, 348, 406, 676, 849, 1069
BsoBI CYCGRG 1 cut(s) 634
Bsp119I TTCGAA 1 cut(s) 35
Bsp120I GGGCCC 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 300, 309, 508
Bsp143I GATC 2 cut(s) 474, 747
BspACI CCGC 5 cut(s) 188, 230, 233, 449, 1066
BspANI GGCC 7 cut(s) 307, 320, 348, 406, 676, 849, 1069
BspCNI CTCAG 2 cut(s) 37, 274
BspDI ATCGAT 1 cut(s) 477
BspLI GGNNCC 5 cut(s) 306, 307, 321, 709, 1102
BspOI GCTAGC 1 cut(s) 500
BspT104I TTCGAA 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 787
BsrI ACTGG 1 cut(s) 603
BssECI CCNNGG 2 cut(s) 635, 910
BssMI GATC 2 cut(s) 474, 747
BssNI GRCGYC 1 cut(s) 490
Bst4CI ACNGT 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 4 cut(s) 192, 494, 498, 738
BstDEI CTNAG 2 cut(s) 24, 282
BstF5I GGATG 4 cut(s) 382, 555, 817, 822
BstHHI GCGC 1 cut(s) 702
BstKTI GATC 2 cut(s) 477, 750
BstMAI GTCTC 2 cut(s) 481, 1050
BstMBI GATC 2 cut(s) 474, 747
BstMWI GCNNNNNNNGC 4 cut(s) 187, 304, 801, 1075
BstSCI CCNGG 1 cut(s) 910
BstSLI GKGCMC 3 cut(s) 300, 309, 508
BstV1I GCAGC 1 cut(s) 804
BstXI CCANNNNNNTGG 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 477
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 7 cut(s) 307, 320, 348, 406, 676, 849, 1069
BsuTUI ATCGAT 1 cut(s) 477
BtsCI GGATG 4 cut(s) 382, 555, 817, 822
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 2 cut(s) 176, 610
Cac8I GCNNGC 4 cut(s) 192, 494, 498, 738
CaiI CAGNNNCTG 2 cut(s) 211, 260
CfoI GCGC 1 cut(s) 702
Cfr13I GGNCC 6 cut(s) 305, 306, 319, 848, 891, 902
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 1 cut(s) 498
Csp6I GTAC 3 cut(s) 258, 766, 1008
CviAII CATG 7 cut(s) 68, 115, 350, 402, 808, 1051, 1090
CviQI GTAC 3 cut(s) 258, 766, 1008
DdeI CTNAG 2 cut(s) 24, 282
DpnI GATC 2 cut(s) 476, 749
DpnII GATC 2 cut(s) 474, 747
EaeI YGGCCR 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 891, 902
Eco57I CTGAAG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 481
FaeI CATG 7 cut(s) 71, 118, 353, 405, 811, 1054, 1093
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 7 cut(s) 67, 114, 349, 401, 807, 1050, 1089
FauNDI CATATG 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 188, 233, 793, 1067
FokI GGATG 4 cut(s) 369, 542, 809, 824
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 4 cut(s) 188, 233, 793, 1067
FspBI CTAG 6 cut(s) 80, 497, 833, 1041, 1097, 1105
GlaI GCGC 1 cut(s) 701
GluI GCNGC 4 cut(s) 188, 233, 793, 1067
HaeIII GGCC 7 cut(s) 307, 320, 348, 406, 676, 849, 1069
HapII CCGG 2 cut(s) 730, 912
HgaI GACGC 1 cut(s) 498
HhaI GCGC 1 cut(s) 702
Hin1I GRCGYC 1 cut(s) 490
Hin1II CATG 7 cut(s) 71, 118, 353, 405, 811, 1054, 1093
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 6 cut(s) 111, 251, 398, 548, 865, 952
HpaII CCGG 2 cut(s) 730, 912
HphI GGTGA 5 cut(s) 142, 219, 494, 565, 899
Hpy166II GTNNAC 3 cut(s) 298, 376, 838
Hpy188I TCNGA 9 cut(s) 17, 277, 547, 666, 694, 757, 870, 940, 957
Hpy8I GTNNAC 3 cut(s) 298, 376, 838
HpyAV CCTTC 5 cut(s) 148, 347, 396, 423, 634
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 9 cut(s) 58, 272, 298, 555, 792, 978, 1001, 1078, 1085
HpyF10VI GCNNNNNNNGC 4 cut(s) 187, 304, 801, 1075
HpyF3I CTNAG 2 cut(s) 24, 282
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 490
Hsp92II CATG 7 cut(s) 71, 118, 353, 405, 811, 1054, 1093
HspAI GCGC 1 cut(s) 700
Kzo9I GATC 2 cut(s) 474, 747
LmnI GCTCC 2 cut(s) 713, 870
Lsp1109I GCAGC 1 cut(s) 804
LweI GCATC 2 cut(s) 281, 564
MaeI CTAG 6 cut(s) 80, 497, 833, 1041, 1097, 1105
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 174, 874
MalI GATC 2 cut(s) 476, 749
MboI GATC 2 cut(s) 474, 747
MboII GAAGA 4 cut(s) 133, 331, 866, 927
MhlI GDGCHC 3 cut(s) 300, 309, 508
MlsI TGGCCA 1 cut(s) 348
MluCI AATT 6 cut(s) 30, 412, 435, 945, 973, 1080
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 3 cut(s) 120, 407, 557
Mox20I TGGCCA 1 cut(s) 348
MscI TGGCCA 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 348
MspI CCGG 2 cut(s) 730, 912
MspR9I CCNGG 1 cut(s) 912
MwoI GCNNNNNNNGC 4 cut(s) 187, 304, 801, 1075
NciI CCSGG 1 cut(s) 912
NdeI CATATG 1 cut(s) 391
NdeII GATC 2 cut(s) 474, 747
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 7 cut(s) 71, 118, 353, 405, 811, 1054, 1093
NlaIV GGNNCC 5 cut(s) 306, 307, 321, 709, 1102
NmuCI GTSAC 1 cut(s) 174
NspV TTCGAA 1 cut(s) 35
PfeI GAWTC 3 cut(s) 251, 865, 952
PkrI GCNGC 4 cut(s) 189, 234, 794, 1068
PleI GAGTC 3 cut(s) 119, 406, 556
PpsI GAGTC 3 cut(s) 119, 406, 556
Psp1406I AACGTT 1 cut(s) 267
PspN4I GGNNCC 5 cut(s) 306, 307, 321, 709, 1102
PspOMI GGGCCC 1 cut(s) 305
PspPI GGNCC 6 cut(s) 305, 306, 319, 848, 891, 902
PstNI CAGNNNCTG 2 cut(s) 211, 260
RsaI GTAC 3 cut(s) 259, 767, 1009
RsaNI GTAC 3 cut(s) 258, 766, 1008
SatI GCNGC 4 cut(s) 188, 233, 793, 1067
Sau3AI GATC 2 cut(s) 474, 747
Sau96I GGNCC 6 cut(s) 305, 306, 319, 848, 891, 902
ScaI AGTACT 2 cut(s) 767, 1009
SchI GAGTC 3 cut(s) 120, 407, 557
ScrFI CCNGG 1 cut(s) 912
SduI GDGCHC 3 cut(s) 300, 309, 508
SfaNI GCATC 2 cut(s) 281, 564
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 2 cut(s) 891, 902
Sse9I AATT 6 cut(s) 30, 412, 435, 945, 973, 1080
SsiI CCGC 5 cut(s) 188, 230, 233, 449, 1066
SspMI CTAG 6 cut(s) 80, 497, 833, 1041, 1097, 1105
StyD4I CCNGG 1 cut(s) 910
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 1 cut(s) 270
TaqI TCGA 5 cut(s) 35, 477, 720, 863, 885
TasI AATT 6 cut(s) 30, 412, 435, 945, 973, 1080
TatI WGTACW 2 cut(s) 765, 1007
TauI GCSGC 3 cut(s) 190, 235, 1069
TfiI GAWTC 3 cut(s) 251, 865, 952
TscAI CASTG 2 cut(s) 183, 610
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 1 cut(s) 792
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 6 cut(s) 65, 114, 366, 378, 612, 1039
TspRI CASTG 2 cut(s) 183, 610
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 2 cut(s) 891, 902
XapI RAATTY 2 cut(s) 412, 435
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XspI CTAG 6 cut(s) 80, 497, 833, 1041, 1097, 1105
ZrmI AGTACT 2 cut(s) 767, 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.