Rroxscaffold_3G00268630

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004921
Physical Location & Seq
Reverse (-)
61691204 .. 61692828
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268630.1

Sequence Viewer

Length: 1215 bp
ATGGGAATTGGAGACCGAAAATCTCAACCAATTAGAAAACTTTTGGGTTCCTATTTACAGTTTTTTCATGCCTTTCATCTCGTGTTCTTTTTCATTGGTTTGTCCATTGGAGTCATAATCAGCTTTTTCTGCAAAAACTTCATATTCAAATTGCAAACCACCTCAGATTCAGTCTCTTCGTTCACACTACCTACGTCAACATCACTGTTGCTGCTTCCGCGGCCACCCTCACCAGCGCCGATCCCTTTACTGGATTCCGGTGATGTCGTTACATCCGATGTTGAGGCTCAGACACTTCTGGTGCACAATATGGATGATGATCTTGAGTTGTTTTGGCGGGCATCGTTTGTTCCGAGGATTACAGAGTTTCCTTATAACCGTGTGCCTAAAGTTGCTTTCATGTTCTTGACAAAGGGTCCTATACCTCTAGCCCCATTGTGGGAAATGTTCTTCAAAGACCATCAAGGACTATACAACATATATTTTCACCCTCATCCATCCTATGTAGAATCGTGGCCCAAAAATTCTGTTTTCTATCGAACAAGAATTCCAAGCAAGCAAGTTGAATGGGGAAAACCAACCATGATTGACGCCGAGAGACGTCTACTAGCGAGTGCCCTCCTTGACTTCTCTAATGAACGATTCGTGTTGTTGTCCGAGACTTGCATCCCACTATTCAACTTCACTACAGTGTACAACTATCTCATGAATTCCAATAAGAGCTTTTTAGCTTCCTATGATGATCCAAGGAAAGTTGGCCGTGGCAGATACAATCCCCAAATGGATCCTACAGTTACAATATCAGATTGGCGAAAAGGGTCACAATGGTTCGAGGTCAACCGCAAACTTGCAATAGAGATCATATCAGATACCAAGTACTACCCCATATTCAAAGAGTATTGCAGTCCTCCTTGCTACATGGATGAGCATTACATCCCTACCCTAGTGAACATTATCAGCCCCGGTCACAACTCAAACAGGACCGTTACTTGGGTTGATTGGTCCAAGAGTGGTCCGCACCCTGGAAGATTTGGGAGGAATGGTGTTTCAGATGAATTGCTGAATCGAATAAGGTTTGGTACTAATGGTACCTGTGATGGTGATGTTAATACAACCTGTTCTATCTGCTTTTTGTTTGCTAGGAAGTTCATGCCGGACACTCTGCAACCTCTCTTGCAAATTGCACCCGTGTTGCTAGGTTCCATTTCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

404

Amino Acids

46.3

Weight (kDa)

8.56

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 131 - 358 4.1e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AatII GACGTC 1 cut(s) 604
Acc65I GGTACC 1 cut(s) 1088
AccB1I GGYRCC 1 cut(s) 1088
AccI GTMKAC 1 cut(s) 604
AccII CGCG 1 cut(s) 220
AciI CCGC 5 cut(s) 218, 220, 337, 841, 1016
AclWI GGATC 4 cut(s) 235, 737, 779, 792
AcoI YGGCCR 2 cut(s) 221, 757
AcsI RAATTY 3 cut(s) 523, 546, 709
AcyI GRCGYC 2 cut(s) 591, 601
AfaI GTAC 4 cut(s) 695, 878, 1081, 1090
AfiI CCNNNNNNNGG 5 cut(s) 250, 438, 439, 990, 1022
AgsI TTSAA 5 cut(s) 148, 454, 566, 679, 892
AhdI GACNNNNNGTC 1 cut(s) 414
AjnI CCWGG 1 cut(s) 1021
AleI CACNNNNGTG 1 cut(s) 689
AloI GAACNNNNNNTCC 2 cut(s) 532, 564
AluBI AGCT 3 cut(s) 123, 723, 731
AluI AGCT 3 cut(s) 123, 723, 731
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 4 cut(s) 6, 178, 592, 653
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 4 cut(s) 235, 737, 779, 792
AoxI GGCC 3 cut(s) 221, 515, 757
ApaLI GTGCAC 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 211
ApoI RAATTY 3 cut(s) 523, 546, 709
Asp718I GGTACC 1 cut(s) 1088
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 5 cut(s) 416, 516, 981, 1002, 1013
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 4 cut(s) 222, 272, 479, 1112
AvaII GGWCC 4 cut(s) 416, 981, 1002, 1013
BaeGI GKGCMC 2 cut(s) 306, 619
BamHI GGATCC 1 cut(s) 784
BanI GGYRCC 1 cut(s) 1088
BauI CACGAG 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 198
BccI CCATC 3 cut(s) 468, 505, 1091
BceAI ACGGC 1 cut(s) 744
BciT130I CCWGG 1 cut(s) 1023
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 4 cut(s) 6, 178, 592, 653
BfaI CTAG 5 cut(s) 428, 608, 944, 1140, 1196
BfmI CTRYAG 2 cut(s) 687, 789
BfoI RGCGCY 1 cut(s) 239
BisI GCNGC 2 cut(s) 212, 221
BlsI GCNGC 2 cut(s) 213, 222
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 2 cut(s) 963, 1023
Bme18I GGWCC 4 cut(s) 416, 981, 1002, 1013
BmeRI GACNNNNNGTC 1 cut(s) 414
BmgT120I GGNCC 5 cut(s) 416, 516, 981, 1002, 1013
BmiI GGNNCC 5 cut(s) 49, 417, 786, 1090, 1201
BmrFI CCNGG 2 cut(s) 963, 1023
BmsI GCATC 2 cut(s) 350, 675
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 963
BsaBI GATNNNNATC 1 cut(s) 318
BsaHI GRCGYC 2 cut(s) 591, 601
BsaI GGTCTC 1 cut(s) 6
BsaJI CCNNGG 6 cut(s) 218, 353, 746, 760, 961, 1021
BsaWI WCCGGW 1 cut(s) 257
BsaXI ACNNNNNCTCC 2 cut(s) 1027, 1057
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 438, 439, 990, 1022
Bse1I ACTGG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 6 cut(s) 218, 353, 746, 760, 961, 1021
BseGI GGATG 7 cut(s) 272, 319, 493, 497, 666, 928, 933
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 5 cut(s) 250, 438, 439, 990, 1022
BseMII CTCAG 2 cut(s) 177, 302
BseNI ACTGG 1 cut(s) 255
BseSI GKGCMC 2 cut(s) 306, 619
BseXI GCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 223, 517, 759
BshNI GGYRCC 1 cut(s) 1088
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 3 cut(s) 258, 963, 1154
BslI CCNNNNNNNGG 5 cut(s) 250, 438, 439, 990, 1022
BsmAI GTCTC 4 cut(s) 6, 178, 592, 653
BsmBI CGTCTC 1 cut(s) 592
BsnI GGCC 3 cut(s) 223, 517, 759
Bso31I GGTCTC 1 cut(s) 6
Bsp1286I GDGCHC 2 cut(s) 306, 619
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 5 cut(s) 240, 319, 742, 784, 858
BspACI CCGC 5 cut(s) 218, 220, 337, 841, 1016
BspANI GGCC 3 cut(s) 223, 517, 759
BspCNI CTCAG 2 cut(s) 176, 301
BspFNI CGCG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 5 cut(s) 49, 417, 786, 1090, 1201
BspPI GGATC 4 cut(s) 235, 737, 779, 792
BspT107I GGYRCC 1 cut(s) 1088
BspTNI GGTCTC 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 6 cut(s) 218, 353, 746, 760, 961, 1021
BssMI GATC 5 cut(s) 240, 319, 742, 784, 858
BssNI GRCGYC 2 cut(s) 591, 601
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 746
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 6 cut(s) 60, 207, 380, 691, 793, 985
Bst6I CTCTTC 1 cut(s) 181
BstACI GRCGYC 2 cut(s) 591, 601
BstAUI TGTACA 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 339, 557
BstDEI CTNAG 2 cut(s) 163, 288
BstDSI CCRYGG 2 cut(s) 218, 760
BstF5I GGATG 7 cut(s) 272, 319, 493, 497, 666, 928, 933
BstFNI CGCG 1 cut(s) 220
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 5 cut(s) 243, 322, 745, 787, 861
BstMAI GTCTC 4 cut(s) 6, 178, 592, 653
BstMBI GATC 5 cut(s) 240, 319, 742, 784, 858
BstMWI GCNNNNNNNGC 3 cut(s) 129, 217, 220
BstNI CCWGG 1 cut(s) 1023
BstSCI CCNGG 2 cut(s) 961, 1021
BstSFI CTRYAG 2 cut(s) 687, 789
BstSLI GKGCMC 2 cut(s) 306, 619
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuRI GGCC 3 cut(s) 223, 517, 759
BtgI CCRYGG 2 cut(s) 218, 760
BtsCI GGATG 7 cut(s) 272, 319, 493, 497, 666, 928, 933
BtsIMutI CAGTG 2 cut(s) 203, 696
Cac8I GCNNGC 2 cut(s) 339, 557
CciI TCATGA 1 cut(s) 705
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 5 cut(s) 416, 516, 981, 1002, 1013
Cfr42I CCGCGG 1 cut(s) 221
CseI GACGC 1 cut(s) 599
Csp6I GTAC 4 cut(s) 694, 877, 1080, 1089
CviAII CATG 6 cut(s) 68, 400, 583, 706, 919, 1150
CviJI RGCY 9 cut(s) 123, 223, 287, 431, 517, 723, 731, 759, 960
CviKI_1 RGCY 9 cut(s) 123, 223, 287, 431, 517, 723, 731, 759, 960
CviQI GTAC 4 cut(s) 694, 877, 1080, 1089
DdeI CTNAG 2 cut(s) 163, 288
DpnI GATC 5 cut(s) 242, 321, 744, 786, 860
DpnII GATC 5 cut(s) 240, 319, 742, 784, 858
DriI GACNNNNNGTC 1 cut(s) 414
EaeI YGGCCR 2 cut(s) 221, 757
Eam1104I CTCTTC 1 cut(s) 181
Eam1105I GACNNNNNGTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 746
Eco31I GGTCTC 1 cut(s) 6
Eco47I GGWCC 4 cut(s) 416, 981, 1002, 1013
EcoO109I RGGNCCY 1 cut(s) 416
EcoRI GAATTC 2 cut(s) 546, 709
EcoRII CCWGG 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 746
ErhI CCWWGG 1 cut(s) 746
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 6 cut(s) 71, 403, 586, 709, 922, 1153
FatI CATG 6 cut(s) 67, 399, 582, 705, 918, 1149
FauI CCCGC 1 cut(s) 330
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 2 cut(s) 212, 221
FokI GGATG 7 cut(s) 259, 326, 480, 484, 653, 920, 935
Fsp4HI GCNGC 2 cut(s) 212, 221
FspBI CTAG 5 cut(s) 428, 608, 944, 1140, 1196
GlaI GCGC 1 cut(s) 237
GluI GCNGC 2 cut(s) 212, 221
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 3 cut(s) 223, 517, 759
HapII CCGG 3 cut(s) 258, 963, 1154
HgaI GACGC 1 cut(s) 599
HhaI GCGC 1 cut(s) 238
Hin1I GRCGYC 2 cut(s) 591, 601
Hin1II CATG 6 cut(s) 71, 403, 586, 709, 922, 1153
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HincII GTYRAC 2 cut(s) 198, 838
HindII GTYRAC 2 cut(s) 198, 838
HinfI GANTC 6 cut(s) 111, 167, 254, 509, 642, 1063
HpaII CCGG 3 cut(s) 258, 963, 1154
HphI GGTGA 4 cut(s) 222, 272, 479, 1112
Hpy166II GTNNAC 7 cut(s) 183, 198, 304, 605, 694, 838, 949
Hpy188I TCNGA 8 cut(s) 166, 277, 291, 354, 658, 805, 868, 1051
Hpy188III TCNNGA 3 cut(s) 323, 406, 706
Hpy8I GTNNAC 7 cut(s) 183, 198, 304, 605, 694, 838, 949
HpyCH4III ACNGT 6 cut(s) 60, 207, 380, 691, 793, 985
HpyCH4IV ACGT 2 cut(s) 194, 601
HpyCH4V TGCA 9 cut(s) 132, 154, 304, 666, 851, 903, 1165, 1177, 1184
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 217, 220
HpyF3I CTNAG 2 cut(s) 163, 288
HpySE526I ACGT 2 cut(s) 194, 601
Hsp92I GRCGYC 2 cut(s) 591, 601
Hsp92II CATG 6 cut(s) 71, 403, 586, 709, 922, 1153
HspAI GCGC 1 cut(s) 236
KpnI GGTACC 1 cut(s) 1092
KspI CCGCGG 1 cut(s) 221
Kzo9I GATC 5 cut(s) 240, 319, 742, 784, 858
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 2 cut(s) 350, 675
MaeI CTAG 5 cut(s) 428, 608, 944, 1140, 1196
MaeII ACGT 2 cut(s) 194, 601
MaeIII GTNAC 5 cut(s) 268, 793, 819, 965, 985
MalI GATC 5 cut(s) 242, 321, 744, 786, 860
MboI GATC 5 cut(s) 240, 319, 742, 784, 858
MboII GAAGA 3 cut(s) 168, 442, 1038
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 306, 619
MluCI AATT 8 cut(s) 6, 30, 149, 523, 546, 709, 1055, 1179
MlyI GAGTC 1 cut(s) 120
MseI TTAA 1 cut(s) 1107
MslI CAYNNNNRTG 1 cut(s) 689
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 3 cut(s) 258, 963, 1154
MspR9I CCNGG 2 cut(s) 963, 1023
MvaI CCWGG 1 cut(s) 1023
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 3 cut(s) 129, 217, 220
NciI CCSGG 1 cut(s) 963
NdeII GATC 5 cut(s) 240, 319, 742, 784, 858
NlaIII CATG 6 cut(s) 71, 403, 586, 709, 922, 1153
NlaIV GGNNCC 5 cut(s) 49, 417, 786, 1090, 1201
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 2 cut(s) 819, 965
OliI CACNNNNGTG 1 cut(s) 689
PagI TCATGA 1 cut(s) 705
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 350
PfeI GAWTC 5 cut(s) 167, 254, 509, 642, 1063
PkrI GCNGC 2 cut(s) 213, 222
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 416
PsiI TTATAA 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 1021
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 5 cut(s) 49, 417, 786, 1090, 1201
PspPI GGNCC 5 cut(s) 416, 516, 981, 1002, 1013
PspPPI RGGWCCY 1 cut(s) 416
PsuI RGATCY 1 cut(s) 784
RsaI GTAC 4 cut(s) 695, 878, 1081, 1090
RsaNI GTAC 4 cut(s) 694, 877, 1080, 1089
RseI CAYNNNNRTG 1 cut(s) 689
SacII CCGCGG 1 cut(s) 221
SaqAI TTAA 1 cut(s) 1107
SatI GCNGC 2 cut(s) 212, 221
Sau3AI GATC 5 cut(s) 240, 319, 742, 784, 858
Sau96I GGNCC 5 cut(s) 416, 516, 981, 1002, 1013
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 963, 1023
SduI GDGCHC 2 cut(s) 306, 619
SfaNI GCATC 2 cut(s) 350, 675
SfcI CTRYAG 2 cut(s) 687, 789
Sfr303I CCGCGG 1 cut(s) 221
SgrBI CCGCGG 1 cut(s) 221
SinI GGWCC 4 cut(s) 416, 981, 1002, 1013
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 8 cut(s) 6, 30, 149, 523, 546, 709, 1055, 1179
SsiI CCGC 5 cut(s) 218, 220, 337, 841, 1016
SspMI CTAG 5 cut(s) 428, 608, 944, 1140, 1196
StyD4I CCNGG 2 cut(s) 961, 1021
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 6 cut(s) 60, 207, 380, 691, 793, 985
TaiI ACGT 2 cut(s) 197, 604
TaqI TCGA 3 cut(s) 538, 831, 1066
TaqII GACCGA 1 cut(s) 30
TasI AATT 8 cut(s) 6, 30, 149, 523, 546, 709, 1055, 1179
TatI WGTACW 2 cut(s) 693, 876
TauI GCSGC 1 cut(s) 223
TfiI GAWTC 5 cut(s) 167, 254, 509, 642, 1063
Tru1I TTAA 1 cut(s) 1107
Tru9I TTAA 1 cut(s) 1107
TscAI CASTG 2 cut(s) 210, 696
TseFI GTSAC 2 cut(s) 819, 965
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 2 cut(s) 819, 965
TspDTI ATGAA 9 cut(s) 56, 65, 82, 130, 388, 651, 722, 1068, 1138
TspRI CASTG 2 cut(s) 210, 696
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 4 cut(s) 416, 981, 1002, 1013
XapI RAATTY 3 cut(s) 523, 546, 709
XmiI GTMKAC 1 cut(s) 604
XspI CTAG 5 cut(s) 428, 608, 944, 1140, 1196
ZraI GACGTC 1 cut(s) 602
ZrmI AGTACT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.