FvH4_5g11820

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004921
Physical Location & Seq
Reverse (-)
6687716 .. 6689317
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g11820.t1

Sequence Viewer

Length: 1212 bp
ATGGGGAATGCAGACCGAAAATCTCAATCTATTCGAAAGCTTTTGAGTTCCTATGTACAGTCTTTTCATGCCTTTCATCTCGTCTTCTTTGTCATTGGCTTGTCGATTGGATTCATAATCAGCTTCTACTGCAAAAACTTCATTTTCAGATTGCAAATCACTTCAAATTCGGTCTCTACTTTGTCACCATTGCTGCTTCCGCCGCCCCCCTCATCCTCACCTTCACCACCGCAGCTCCCTTTAATGGATACCGGCGATGTTGTTACATCCAATGTTGAAGCTCAGACACTTTTGATGCACAATTTGGATGATGATCTTGAGTTGTTTTGGCGGGCTTCGTTTGTTCCAAGAATTACCGAGTTTCCCTATAACCGTGTGCCTAAAGTTGCTTTCATGTTCTTGACAAAGGGTCGTATACCTCTGGCTCCACTGTGGGAAATGTTCTTCAAAGACCATCAAGGACTTTACACCATATATGTTCATCCCCATCCATCCTATGTAGACTCATGGCCTGAAGATTCTGCTTTCTATCGAACAAGAATTCCAAGCAAGCAAGTCGATTGGGGAAAACCAACCATGATTGACGCCGAGAGACGCCTGCTAGCGAGTGCCCTCCTCGACATCTCTAACGAACGATTCGTGTTGTTGTCTGAGACATGCATCCCATTATTCAACTTCACCACAATATACAACTGTCTCATGAATTCCAATGAGAGCTTTATAGCTTCCTATGATGATCCAAGGAAGGTTGGCCGTGGGAGATACAATCCCCAAATGGCTCCTATGATCACAGTAGCGGATTGGCGAAAAGGGTCACAATGGTTCGAGGTCAACCGCAAGCTTGCGATAGAGATCGTATCAGATACCAAGTACTACCCCATATTCAAAGAGTACTGCAGTCATCCTTGCTACATGGATGAGCACTACATCCCTACCTTAGTGAACATTATCGGACCCGGTCACAACTCGAACCGGAGCGTTACTTGGGTTGATTGGTCCAAGAGTGGTCCGCACCCTGGAAGATTTGGGAGGAGTCATGTTTCAGCTGAAGTGCTGAATCAAATACGGTTTGGCACAAATGAAACCTGTGATCATGGTGATGTTAATATAACCAGTTCTATGTGCTTCTTGTTTGCTAGGAAGTTCATGCCGGACGCTCTGCAGCCTCTGTTGCAAATTGTACCCATGTTAGTAGGTTCAAATTCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

404

Amino Acids

46.1

Weight (kDa)

7.66

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 129 - 356 1.3e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 415, 501
AciI CCGC 7 cut(s) 200, 203, 230, 331, 797, 835, 1010
AclWI GGATC 1 cut(s) 731
AcoI YGGCCR 1 cut(s) 751
AcsI RAATTY 4 cut(s) 166, 540, 703, 1201
AcuI CTGAAG 2 cut(s) 534, 1068
AcyI GRCGYC 2 cut(s) 585, 595
AfaI GTAC 4 cut(s) 57, 872, 893, 1182
AfiI CCNNNNNNNGG 2 cut(s) 244, 1016
AgsI TTSAA 6 cut(s) 165, 278, 448, 673, 886, 1200
AhdI GACNNNNNGTC 1 cut(s) 408
AjnI CCWGG 1 cut(s) 1015
AloI GAACNNNNNNTCC 2 cut(s) 526, 558
AluBI AGCT 8 cut(s) 40, 123, 235, 281, 717, 725, 841, 1046
AluI AGCT 8 cut(s) 40, 123, 235, 281, 717, 725, 841, 1046
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 4 cut(s) 178, 586, 647, 701
AlwI GGATC 1 cut(s) 731
AlwNI CAGNNNCTG 1 cut(s) 1168
AoxI GGCC 2 cut(s) 509, 751
ApeKI GCWGC 3 cut(s) 193, 232, 1162
ApoI RAATTY 4 cut(s) 166, 540, 703, 1201
AspS9I GGNCC 3 cut(s) 953, 996, 1007
AsuC2I CCSGG 1 cut(s) 957
AsuHPI GGTGA 5 cut(s) 177, 210, 216, 670, 1109
AsuII TTCGAA 1 cut(s) 34
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 3 cut(s) 953, 996, 1007
BaeGI GKGCMC 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 3 cut(s) 180, 244, 1174
BccI CCATC 3 cut(s) 462, 495, 499
BceAI ACGGC 1 cut(s) 738
BcgI CGANNNNNNTGC 2 cut(s) 538, 572
BciT130I CCWGG 1 cut(s) 1017
BciVI GTATCC 1 cut(s) 241
BclI TGATCA 2 cut(s) 786, 1090
BcnI CCSGG 1 cut(s) 957
BcoDI GTCTC 4 cut(s) 178, 586, 647, 701
BfaI CTAG 2 cut(s) 602, 1137
BfmI CTRYAG 2 cut(s) 895, 1160
BfuI GTATCC 1 cut(s) 241
BisI GCNGC 4 cut(s) 194, 203, 233, 1163
BlsI GCNGC 4 cut(s) 195, 204, 234, 1164
BmcAI AGTACT 2 cut(s) 872, 893
Bme1390I CCNGG 2 cut(s) 957, 1017
Bme18I GGWCC 3 cut(s) 953, 996, 1007
BmeRI GACNNNNNGTC 1 cut(s) 408
BmgT120I GGNCC 3 cut(s) 953, 996, 1007
BmiI GGNNCC 3 cut(s) 426, 780, 955
BmrFI CCNGG 2 cut(s) 957, 1017
BmsI GCATC 2 cut(s) 285, 669
BmtI GCTAGC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 76
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 957
BsaBI GATNNNNATC 2 cut(s) 312, 851
BsaHI GRCGYC 2 cut(s) 585, 595
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 740, 754, 1015
BsaWI WCCGGW 1 cut(s) 972
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 1016
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 1113
Bse3DI GCAATG 1 cut(s) 188
Bse8I GATNNNNATC 2 cut(s) 312, 851
BseBI CCWGG 1 cut(s) 1017
BseDI CCNNGG 3 cut(s) 740, 754, 1015
BseJI GATNNNNATC 2 cut(s) 312, 851
BseLI CCNNNNNNNGG 2 cut(s) 244, 1016
BseMI GCAATG 1 cut(s) 188
BseMII CTCAG 2 cut(s) 296, 642
BseNI ACTGG 1 cut(s) 1113
BseRI GAGGAG 2 cut(s) 605, 1045
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 3 cut(s) 180, 244, 1174
BshFI GGCC 2 cut(s) 511, 753
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 4 cut(s) 252, 957, 973, 1151
BslI CCNNNNNNNGG 2 cut(s) 244, 1016
BsmAI GTCTC 4 cut(s) 178, 586, 647, 701
BsmBI CGTCTC 1 cut(s) 586
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 2 cut(s) 511, 753
Bso31I GGTCTC 1 cut(s) 178
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 2 cut(s) 613, 924
Bsp1407I TGTACA 1 cut(s) 55
Bsp143I GATC 5 cut(s) 313, 736, 786, 852, 1090
BspACI CCGC 7 cut(s) 200, 203, 230, 331, 797, 835, 1010
BspANI GGCC 2 cut(s) 511, 753
BspCNI CTCAG 2 cut(s) 295, 643
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 3 cut(s) 426, 780, 955
BspMAI CTGCAG 2 cut(s) 899, 1164
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 1 cut(s) 731
BspT104I TTCGAA 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 188
BsrFI RCCGGY 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 55
BsrI ACTGG 1 cut(s) 1113
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 3 cut(s) 740, 754, 1015
BssMI GATC 5 cut(s) 313, 736, 786, 852, 1090
BssNAI GTATAC 1 cut(s) 416
BssNI GRCGYC 2 cut(s) 585, 595
BssT1I CCWWGG 1 cut(s) 740
Bst1107I GTATAC 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 6 cut(s) 60, 374, 432, 695, 793, 1068
BstACI GRCGYC 2 cut(s) 585, 595
BstAUI TGTACA 1 cut(s) 55
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 6 cut(s) 333, 551, 599, 603, 839, 843
BstDEI CTNAG 3 cut(s) 282, 651, 937
BstDSI CCRYGG 1 cut(s) 754
BstKTI GATC 5 cut(s) 316, 739, 789, 855, 1093
BstMAI GTCTC 4 cut(s) 178, 586, 647, 701
BstMBI GATC 5 cut(s) 313, 736, 786, 852, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 129, 199, 202, 1171
BstNI CCWGG 1 cut(s) 1017
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 2 cut(s) 955, 1015
BstSFI CTRYAG 2 cut(s) 895, 1160
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 180, 244, 1174
BstV2I GAAGAC 1 cut(s) 76
BstZ17I GTATAC 1 cut(s) 416
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 511, 753
BtgI CCRYGG 1 cut(s) 754
BtgZI GCGATG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 6 cut(s) 333, 551, 599, 603, 839, 843
CaiI CAGNNNCTG 1 cut(s) 1168
CciI TCATGA 1 cut(s) 699
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 953, 996, 1007
CseI GACGC 3 cut(s) 593, 603, 1163
Csp6I GTAC 4 cut(s) 56, 871, 892, 1181
CviQI GTAC 4 cut(s) 56, 871, 892, 1181
DdeI CTNAG 3 cut(s) 282, 651, 937
DpnI GATC 5 cut(s) 315, 738, 788, 854, 1092
DpnII GATC 5 cut(s) 313, 736, 786, 852, 1090
DriI GACNNNNNGTC 1 cut(s) 408
EaeI YGGCCR 1 cut(s) 751
Eam1105I GACNNNNNGTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 740
Eco31I GGTCTC 1 cut(s) 178
Eco47I GGWCC 3 cut(s) 953, 996, 1007
Eco57I CTGAAG 2 cut(s) 534, 1068
EcoRI GAATTC 2 cut(s) 540, 703
EcoRII CCWGG 1 cut(s) 1015
EcoT14I CCWWGG 1 cut(s) 740
EcoT22I ATGCAT 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 740
Esp3I CGTCTC 1 cut(s) 586
FauI CCCGC 1 cut(s) 324
FbaI TGATCA 2 cut(s) 786, 1090
FblI GTMKAC 2 cut(s) 415, 501
Fnu4HI GCNGC 4 cut(s) 194, 203, 233, 1163
Fsp4HI GCNGC 4 cut(s) 194, 203, 233, 1163
FspBI CTAG 2 cut(s) 602, 1137
GluI GCNGC 4 cut(s) 194, 203, 233, 1163
HaeIII GGCC 2 cut(s) 511, 753
HapII CCGG 4 cut(s) 252, 957, 973, 1151
HgaI GACGC 3 cut(s) 593, 603, 1163
Hin1I GRCGYC 2 cut(s) 585, 595
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 2 cut(s) 38, 839
HinfI GANTC 6 cut(s) 111, 503, 518, 636, 1033, 1057
HpaII CCGG 4 cut(s) 252, 957, 973, 1151
HphI GGTGA 5 cut(s) 177, 210, 216, 670, 1109
Hpy166II GTNNAC 4 cut(s) 416, 502, 832, 943
Hpy188I TCNGA 5 cut(s) 149, 285, 652, 862, 953
Hpy188III TCNNGA 3 cut(s) 317, 400, 700
Hpy8I GTNNAC 4 cut(s) 416, 502, 832, 943
HpyAV CCTTC 2 cut(s) 231, 739
HpyCH4III ACNGT 6 cut(s) 60, 374, 432, 695, 793, 1068
HpyCH4V TGCA 8 cut(s) 11, 132, 154, 298, 660, 897, 1162, 1174
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 199, 202, 1171
HpyF3I CTNAG 3 cut(s) 282, 651, 937
Hsp92I GRCGYC 2 cut(s) 585, 595
Ksp22I TGATCA 2 cut(s) 786, 1090
Kzo9I GATC 5 cut(s) 313, 736, 786, 852, 1090
LmnI GCTCC 4 cut(s) 240, 430, 784, 975
Lsp1109I GCAGC 3 cut(s) 180, 244, 1174
LweI GCATC 2 cut(s) 285, 669
MaeI CTAG 2 cut(s) 602, 1137
MaeIII GTNAC 5 cut(s) 183, 262, 813, 959, 979
MalI GATC 5 cut(s) 315, 738, 788, 854, 1092
MboI GATC 5 cut(s) 313, 736, 786, 852, 1090
MboII GAAGA 4 cut(s) 76, 436, 527, 1032
MhlI GDGCHC 2 cut(s) 613, 924
MluCI AATT 7 cut(s) 166, 301, 351, 540, 703, 1176, 1201
MlyI GAGTC 2 cut(s) 497, 1042
MnlI CCTC 8 cut(s) 220, 226, 429, 623, 626, 820, 1023, 1176
Mph1103I ATGCAT 1 cut(s) 662
MseI TTAA 2 cut(s) 242, 1104
MslI CAYNNNNRTG 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 4 cut(s) 252, 957, 973, 1151
MspR9I CCNGG 2 cut(s) 957, 1017
Mva1269I GAATGC 1 cut(s) 13
MvaI CCWGG 1 cut(s) 1017
MwoI GCNNNNNNNGC 4 cut(s) 129, 199, 202, 1171
NciI CCSGG 1 cut(s) 957
NdeII GATC 5 cut(s) 313, 736, 786, 852, 1090
NheI GCTAGC 1 cut(s) 601
NlaIV GGNNCC 3 cut(s) 426, 780, 955
NmeAIII GCCGAG 1 cut(s) 613
NmuCI GTSAC 3 cut(s) 183, 813, 959
NsiI ATGCAT 1 cut(s) 662
NspI RCATGY 1 cut(s) 660
NspV TTCGAA 1 cut(s) 34
PagI TCATGA 1 cut(s) 699
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PctI GAATGC 1 cut(s) 13
PfeI GAWTC 4 cut(s) 111, 518, 636, 1057
PflFI GACNNNGTC 1 cut(s) 957
PkrI GCNGC 4 cut(s) 195, 204, 234, 1164
PleI GAGTC 2 cut(s) 497, 1041
PpsI GAGTC 2 cut(s) 497, 1041
Psp6I CCWGG 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1015
PspN4I GGNNCC 3 cut(s) 426, 780, 955
PspPI GGNCC 3 cut(s) 953, 996, 1007
PstI CTGCAG 2 cut(s) 899, 1164
PstNI CAGNNNCTG 1 cut(s) 1168
PsyI GACNNNGTC 1 cut(s) 957
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 4 cut(s) 57, 872, 893, 1182
RsaNI GTAC 4 cut(s) 56, 871, 892, 1181
RseI CAYNNNNRTG 1 cut(s) 1098
SaqAI TTAA 2 cut(s) 242, 1104
SatI GCNGC 4 cut(s) 194, 203, 233, 1163
Sau3AI GATC 5 cut(s) 313, 736, 786, 852, 1090
Sau96I GGNCC 3 cut(s) 953, 996, 1007
ScaI AGTACT 2 cut(s) 872, 893
SchI GAGTC 2 cut(s) 497, 1042
ScrFI CCNGG 2 cut(s) 957, 1017
SduI GDGCHC 2 cut(s) 613, 924
SfaNI GCATC 2 cut(s) 285, 669
SfcI CTRYAG 2 cut(s) 895, 1160
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 3 cut(s) 953, 996, 1007
SmiMI CAYNNNNRTG 1 cut(s) 1098
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 7 cut(s) 166, 301, 351, 540, 703, 1176, 1201
SsiI CCGC 7 cut(s) 200, 203, 230, 331, 797, 835, 1010
SspMI CTAG 2 cut(s) 602, 1137
StyD4I CCNGG 2 cut(s) 955, 1015
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 6 cut(s) 60, 374, 432, 695, 793, 1068
TaqI TCGA 7 cut(s) 34, 104, 532, 558, 618, 825, 968
TaqII GACCGA 2 cut(s) 30, 160
TasI AATT 7 cut(s) 166, 301, 351, 540, 703, 1176, 1201
TatI WGTACW 3 cut(s) 55, 870, 891
TauI GCSGC 1 cut(s) 205
TfiI GAWTC 4 cut(s) 111, 518, 636, 1057
Tru1I TTAA 2 cut(s) 242, 1104
Tru9I TTAA 2 cut(s) 242, 1104
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 3 cut(s) 183, 813, 959
TseI GCWGC 3 cut(s) 193, 232, 1162
Tsp45I GTSAC 3 cut(s) 183, 813, 959
TspDTI ATGAA 9 cut(s) 56, 65, 103, 130, 382, 470, 716, 1095, 1135
TspRI CASTG 1 cut(s) 435
Tth111I GACNNNGTC 1 cut(s) 957
VpaK11BI GGWCC 3 cut(s) 953, 996, 1007
XapI RAATTY 4 cut(s) 166, 540, 703, 1201
XceI RCATGY 1 cut(s) 660
XmiI GTMKAC 2 cut(s) 415, 501
XspI CTAG 2 cut(s) 602, 1137
ZrmI AGTACT 2 cut(s) 872, 893
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.