RchiOBHm_Chr4g0434761

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004921
Physical Location & Seq
Reverse (-)
58425813 .. 58427426
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40321

Sequence Viewer

Length: 1230 bp
ATGAAGCAGGGTAAGCTCACAGTGTTTGTAAAGGACCTTTCCACAGGCTATTTGCATGTTAAAACCACTATCATACTCTCTTTGTCTGTATTATTGCTTCTTTTCGTTCTGGGTATGTTCATTATCGGTCACACTACGAAGGTCTTAAGTTCAGAGGTCTACTTCATCCCCCAGCTGAAGACATTTTCCCCACTCACAGCTAATTGCACTAGCCACGCACAATGTCAATGCAACTTTTCTGCGTCTCCGCAGTTACTCCCTATTAGTTCTCTTCCAAATATAAGAGATTACATAGCTCCCAAAGAGCTATGGCACTCCATGAGTGATGAGGAGCTAATATGGCGAGCCTCTATGGTGCCACAGGTCACCGAATACCCTTATAATCGAACGCCAAAGGTCGCATTCATGTTCCTTGCAAAGGGAAAACTGCCATTGGCGCCTCTTTGGGAAAGGTTTTTCATGGGACATGAAGGTTTCTTCTCAGTTTATCTCCATGCAGCACCGGACTTTAAGAACGAGCCCCCCGAATCCTCAGTGTTTTACAATCGGAGGATACCAAGTAAGGCAGTTGAATGGGGAAAGGCCACAATGGTTGAAGCAGAGAGGCGCCTATTAGCCAATGCCTTACTTGACTTCTCAAATGAGAGATTTGTACTACTCTCTGAATCATGCGTACCTCTCTTCAACTTCACCACAATCTATGACTACCTCATCAACTCGAACTACAGTTTCATTAGCTCATTCGATGACCCGAGAGGCACCGGCCGGGGGCGCTACAACAGGCGAATGTGGCCAACCGTGACATTGTCCGATTGGCGCAAGGGTTCACAATGGTTCGAAGTTCACAGAAACCTTGCCCTGAAGATAGTATCAGATGTAACCTACTACCCTATATTTAAGGACCTCTGCCAGCCTCCTTGTTACAACGATGAACACTATTTGGCAACTCTGGTGAGCAAAGTTGGCCCCGGCAAGAACTCAAATAGGAGCATTACCTGGGTTGATTGGTCCGTAGGTGGGCCGCACCCTGCAACATATGGGAGGCAACATGTGACAGAAGCGTTTCTGAATCAGTTGAGACATGGGTTTGATTGTACCTACAATGGTGGCATTAGTAACATTTGCCACCTCTTTGCTAGGAAATTCCATCCTAGTACAGTTGAGCCTCTGCTAAAAATAGCTCCGACTCTGTTTGGATTTAACACCGATAATATTACCACTACAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

409

Amino Acids

46.7

Weight (kDa)

8.96

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 133 - 360 7.9e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AccB1I GGYRCC 4 cut(s) 355, 436, 606, 758
AccI GTMKAC 1 cut(s) 159
AciI CCGC 2 cut(s) 248, 1022
AcoI YGGCCR 2 cut(s) 763, 791
AcsI RAATTY 1 cut(s) 1142
AcuI CTGAAG 2 cut(s) 197, 881
AcyI GRCGYC 2 cut(s) 437, 607
AfaI GTAC 4 cut(s) 654, 675, 1096, 1156
AfiI CCNNNNNNNGG 3 cut(s) 418, 768, 1017
AflII CTTAAG 1 cut(s) 145
AflIII ACRYGT 1 cut(s) 1048
AgsI TTSAA 3 cut(s) 572, 596, 685
AjnI CCWGG 1 cut(s) 995
AluBI AGCT 8 cut(s) 16, 175, 200, 296, 307, 334, 738, 1181
AluI AGCT 8 cut(s) 16, 175, 200, 296, 307, 334, 738, 1181
Alw26I GTCTC 2 cut(s) 249, 1072
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 5 cut(s) 582, 763, 791, 964, 1019
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 1 cut(s) 1142
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 4 cut(s) 439, 609, 774, 819
AspS9I GGNCC 5 cut(s) 34, 901, 965, 1008, 1019
AsuC2I CCSGG 2 cut(s) 767, 969
AsuHPI GGTGA 3 cut(s) 358, 682, 964
AsuII TTCGAA 1 cut(s) 837
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 3 cut(s) 34, 901, 1008
BalI TGGCCA 1 cut(s) 793
BanI GGYRCC 4 cut(s) 355, 436, 606, 758
BanII GRGCYC 1 cut(s) 522
BarI GAAGNNNNNNTAC 2 cut(s) 81, 113
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 509
BccI CCATC 1 cut(s) 1155
BciT130I CCWGG 1 cut(s) 997
BciVI GTATCC 1 cut(s) 546
BcnI CCSGG 2 cut(s) 767, 969
BcoDI GTCTC 2 cut(s) 249, 1072
BfaI CTAG 3 cut(s) 210, 1137, 1152
BfmI CTRYAG 1 cut(s) 724
BfoI RGCGCY 3 cut(s) 440, 610, 775
BfrI CTTAAG 1 cut(s) 145
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 2 cut(s) 498, 1022
BlsI GCNGC 2 cut(s) 499, 1023
Bme1390I CCNGG 3 cut(s) 767, 969, 997
Bme18I GGWCC 3 cut(s) 34, 901, 1008
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 5 cut(s) 34, 901, 965, 1008, 1019
BmiI GGNNCC 5 cut(s) 357, 438, 608, 760, 967
BmrFI CCNGG 3 cut(s) 767, 969, 997
BpiI GAAGAC 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 837
BpuMI CCSGG 2 cut(s) 767, 969
BsaHI GRCGYC 2 cut(s) 437, 607
BsaJI CCNNGG 3 cut(s) 766, 967, 996
BsaWI WCCGGW 1 cut(s) 502
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 768, 1017
Bse118I RCCGGY 1 cut(s) 761
BseBI CCWGG 1 cut(s) 997
BseDI CCNNGG 3 cut(s) 766, 967, 996
BseGI GGATG 2 cut(s) 165, 1147
BseLI CCNNNNNNNGG 3 cut(s) 418, 768, 1017
BseMII CTCAG 2 cut(s) 495, 546
BseRI GAGGAG 1 cut(s) 344
BseX3I CGGCCG 1 cut(s) 763
BseXI GCAGC 1 cut(s) 509
BseYI CCCAGC 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 766
BshFI GGCC 5 cut(s) 584, 765, 793, 966, 1021
BshNI GGYRCC 4 cut(s) 355, 436, 606, 758
BsiEI CGRYCG 1 cut(s) 766
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 4 cut(s) 503, 762, 766, 969
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 3 cut(s) 418, 768, 1017
BsmAI GTCTC 2 cut(s) 249, 1072
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 477
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 5 cut(s) 584, 765, 793, 966, 1021
BsoBI CYCGRG 1 cut(s) 751
Bsp119I TTCGAA 1 cut(s) 837
Bsp1286I GDGCHC 1 cut(s) 522
BspACI CCGC 2 cut(s) 248, 1022
BspANI GGCC 5 cut(s) 584, 765, 793, 966, 1021
BspCNI CTCAG 2 cut(s) 494, 545
BspLI GGNNCC 5 cut(s) 357, 438, 608, 760, 967
BspT104I TTCGAA 1 cut(s) 837
BspT107I GGYRCC 4 cut(s) 355, 436, 606, 758
BspTI CTTAAG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 3 cut(s) 766, 967, 996
BssNI GRCGYC 2 cut(s) 437, 607
Bst2UI CCWGG 1 cut(s) 997
Bst4CI ACNGT 4 cut(s) 22, 728, 799, 1159
Bst6I CTCTTC 2 cut(s) 276, 686
BstACI GRCGYC 2 cut(s) 437, 607
BstAFI CTTAAG 1 cut(s) 145
BstBI TTCGAA 1 cut(s) 837
BstC8I GCNNGC 2 cut(s) 345, 911
BstDEI CTNAG 2 cut(s) 481, 532
BstEII GGTNACC 1 cut(s) 364
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 2 cut(s) 165, 1147
BstH2I RGCGCY 3 cut(s) 440, 610, 775
BstHHI GCGC 4 cut(s) 439, 609, 774, 819
BstMAI GTCTC 2 cut(s) 249, 1072
BstMCI CGRYCG 1 cut(s) 766
BstMWI GCNNNNNNNGC 6 cut(s) 13, 340, 436, 771, 790, 963
BstNI CCWGG 1 cut(s) 997
BstNSI RCATGY 2 cut(s) 59, 1052
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 3 cut(s) 765, 967, 995
BstSFI CTRYAG 1 cut(s) 724
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 763
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 5 cut(s) 584, 765, 793, 966, 1021
BtsCI GGATG 2 cut(s) 165, 1147
BtsIMutI CAGTG 2 cut(s) 27, 540
Cac8I GCNNGC 2 cut(s) 345, 911
CfoI GCGC 4 cut(s) 439, 609, 774, 819
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 5 cut(s) 34, 901, 965, 1008, 1019
CseI GACGC 1 cut(s) 231
Csp6I GTAC 4 cut(s) 653, 674, 1095, 1155
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 9 cut(s) 56, 319, 406, 460, 467, 494, 669, 1049, 1082
CviQI GTAC 4 cut(s) 653, 674, 1095, 1155
DdeI CTNAG 2 cut(s) 481, 532
DinI GGCGCC 2 cut(s) 438, 608
EaeI YGGCCR 2 cut(s) 763, 791
EagI CGGCCG 1 cut(s) 763
Eam1104I CTCTTC 2 cut(s) 276, 686
EarI CTCTTC 2 cut(s) 276, 686
EclXI CGGCCG 1 cut(s) 763
Eco24I GRGCYC 1 cut(s) 522
Eco47I GGWCC 3 cut(s) 34, 901, 1008
Eco52I CGGCCG 1 cut(s) 763
Eco57I CTGAAG 2 cut(s) 197, 881
Eco88I CYCGRG 1 cut(s) 751
Eco91I GGTNACC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoO109I RGGNCCY 2 cut(s) 34, 901
EcoO65I GGTNACC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 995
EcoT38I GRGCYC 1 cut(s) 522
EgeI GGCGCC 2 cut(s) 438, 608
EheI GGCGCC 2 cut(s) 438, 608
Esp3I CGTCTC 1 cut(s) 249
FaeI CATG 9 cut(s) 59, 322, 409, 463, 470, 497, 672, 1052, 1085
FaqI GGGAC 1 cut(s) 477
FatI CATG 9 cut(s) 55, 318, 405, 459, 466, 493, 668, 1048, 1081
FauNDI CATATG 1 cut(s) 1036
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 2 cut(s) 498, 1022
FokI GGATG 2 cut(s) 152, 1134
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 2 cut(s) 498, 1022
FspBI CTAG 3 cut(s) 210, 1137, 1152
GlaI GCGC 4 cut(s) 438, 608, 773, 818
GluI GCNGC 2 cut(s) 498, 1022
GsaI CCCAGC 1 cut(s) 175
HaeII RGCGCY 3 cut(s) 440, 610, 775
HaeIII GGCC 5 cut(s) 584, 765, 793, 966, 1021
HapII CCGG 4 cut(s) 503, 762, 766, 969
HgaI GACGC 1 cut(s) 231
HhaI GCGC 4 cut(s) 439, 609, 774, 819
Hin1I GRCGYC 2 cut(s) 437, 607
Hin1II CATG 9 cut(s) 59, 322, 409, 463, 470, 497, 672, 1052, 1085
Hin6I GCGC 4 cut(s) 437, 607, 772, 817
HinP1I GCGC 4 cut(s) 437, 607, 772, 817
HinfI GANTC 4 cut(s) 527, 665, 1069, 1186
HpaII CCGG 4 cut(s) 503, 762, 766, 969
HphI GGTGA 3 cut(s) 358, 682, 964
Hpy166II GTNNAC 3 cut(s) 160, 827, 844
Hpy188I TCNGA 7 cut(s) 154, 549, 664, 811, 874, 1068, 1185
Hpy8I GTNNAC 3 cut(s) 160, 827, 844
HpyAV CCTTC 2 cut(s) 133, 464
HpyCH4III ACNGT 4 cut(s) 22, 728, 799, 1159
HpyCH4V TGCA 6 cut(s) 55, 207, 231, 416, 497, 1031
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 340, 436, 771, 790, 963
HpyF3I CTNAG 2 cut(s) 481, 532
Hsp92I GRCGYC 2 cut(s) 437, 607
Hsp92II CATG 9 cut(s) 59, 322, 409, 463, 470, 497, 672, 1052, 1085
HspAI GCGC 4 cut(s) 437, 607, 772, 817
KasI GGCGCC 2 cut(s) 436, 606
LmnI GCTCC 4 cut(s) 301, 331, 987, 1186
Lsp1109I GCAGC 1 cut(s) 509
MaeI CTAG 3 cut(s) 210, 1137, 1152
MaeIII GTNAC 8 cut(s) 128, 252, 364, 799, 877, 920, 1051, 1115
MboII GAAGA 5 cut(s) 190, 263, 469, 673, 874
MhlI GDGCHC 1 cut(s) 522
MlsI TGGCCA 1 cut(s) 793
MluCI AATT 2 cut(s) 202, 1142
MluNI TGGCCA 1 cut(s) 793
Mly113I GGCGCC 2 cut(s) 437, 607
MlyI GAGTC 1 cut(s) 1180
MmeI TCCRAC 1 cut(s) 1208
Mox20I TGGCCA 1 cut(s) 793
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 793
MseI TTAA 5 cut(s) 60, 146, 510, 897, 1200
Msp20I TGGCCA 1 cut(s) 793
MspA1I CMGCKG 1 cut(s) 175
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 4 cut(s) 503, 762, 766, 969
MspR9I CCNGG 3 cut(s) 767, 969, 997
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 1 cut(s) 997
MwoI GCNNNNNNNGC 6 cut(s) 13, 340, 436, 771, 790, 963
NarI GGCGCC 2 cut(s) 437, 607
NciI CCSGG 2 cut(s) 767, 969
NdeI CATATG 1 cut(s) 1036
NlaIII CATG 9 cut(s) 59, 322, 409, 463, 470, 497, 672, 1052, 1085
NlaIV GGNNCC 5 cut(s) 357, 438, 608, 760, 967
NmuCI GTSAC 4 cut(s) 128, 364, 799, 1051
NspI RCATGY 2 cut(s) 59, 1052
NspV TTCGAA 1 cut(s) 837
PciI ACATGT 1 cut(s) 1048
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 3 cut(s) 527, 665, 1069
PflFI GACNNNGTC 1 cut(s) 805
PkrI GCNGC 2 cut(s) 499, 1023
PleI GAGTC 1 cut(s) 1180
PluTI GGCGCC 2 cut(s) 440, 610
PpsI GAGTC 1 cut(s) 1180
PpuMI RGGWCCY 2 cut(s) 34, 901
PscI ACATGT 1 cut(s) 1048
PsiI TTATAA 1 cut(s) 381
Psp5II RGGWCCY 2 cut(s) 34, 901
Psp6I CCWGG 1 cut(s) 995
PspEI GGTNACC 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 995
PspN4I GGNNCC 5 cut(s) 357, 438, 608, 760, 967
PspPI GGNCC 5 cut(s) 34, 901, 965, 1008, 1019
PspPPI RGGWCCY 2 cut(s) 34, 901
PsyI GACNNNGTC 1 cut(s) 805
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 4 cut(s) 654, 675, 1096, 1156
RsaNI GTAC 4 cut(s) 653, 674, 1095, 1155
SaqAI TTAA 5 cut(s) 60, 146, 510, 897, 1200
SatI GCNGC 2 cut(s) 498, 1022
Sau96I GGNCC 5 cut(s) 34, 901, 965, 1008, 1019
SchI GAGTC 1 cut(s) 1180
ScrFI CCNGG 3 cut(s) 767, 969, 997
SduI GDGCHC 1 cut(s) 522
SfcI CTRYAG 1 cut(s) 724
SfoI GGCGCC 2 cut(s) 438, 608
SfuI TTCGAA 1 cut(s) 837
SinI GGWCC 3 cut(s) 34, 901, 1008
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 2 cut(s) 202, 1142
SsiI CCGC 2 cut(s) 248, 1022
SspDI GGCGCC 2 cut(s) 436, 606
SspI AATATT 1 cut(s) 1213
SspMI CTAG 3 cut(s) 210, 1137, 1152
StyD4I CCNGG 3 cut(s) 765, 967, 995
TaaI ACNGT 4 cut(s) 22, 728, 799, 1159
TaqI TCGA 4 cut(s) 385, 719, 744, 837
TaqII GACCGA 1 cut(s) 116
TasI AATT 2 cut(s) 202, 1142
TatI WGTACW 2 cut(s) 652, 1154
TauI GCSGC 1 cut(s) 1024
TfiI GAWTC 3 cut(s) 527, 665, 1069
Tru1I TTAA 5 cut(s) 60, 146, 510, 897, 1200
Tru9I TTAA 5 cut(s) 60, 146, 510, 897, 1200
TscAI CASTG 2 cut(s) 27, 540
TseFI GTSAC 4 cut(s) 128, 364, 799, 1051
TseI GCWGC 1 cut(s) 497
Tsp45I GTSAC 4 cut(s) 128, 364, 799, 1051
TspDTI ATGAA 8 cut(s) 17, 109, 154, 394, 448, 483, 721, 945
TspGWI ACGGA 1 cut(s) 1000
TspRI CASTG 2 cut(s) 27, 540
Tth111I GACNNNGTC 1 cut(s) 805
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 3 cut(s) 34, 901, 1008
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 1 cut(s) 1142
XceI RCATGY 2 cut(s) 59, 1052
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 3 cut(s) 210, 1137, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.