pycom14g18970

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004921
Physical Location & Seq
Forward (+)
20488158 .. 20489507
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g18970.1

Sequence Viewer

Length: 1212 bp
ATGGGAAGCGAACTCCGAAAATCTCAGCCAATTTCGAAGCTTTTGGTTTCGTATTTGCAGTTTAGCCATGCCTTTCATCTAGTGTTTTTTGTCATTGGTTTGTCCATTGGAGTCATGGTCAGTTCATACTACAAGACCTTCTTCCCATTCACCTTACAAACCACTTCCTCCTCGTCACTGCTATTGCCGCCTGCCTCAATGACAGAGTTTCTGCCACAACCACCCTCACCGCCGCTTCTCCCTTTACAGGAATCAGGTACTGATAACGTTGCATCGGATACTGAGGCACTTTTGGTGCACAAGATGGTTGATGATGGTGAGTTGTTCTGGAGAGCATCTTTTGTTCCAAGGATTGGAAAGTTTCCATATAACCGTGTGCCTAGAATCGCCTTCATGTTTTTGACAAAGGGGCCCATACCTTTGGCCCCATTATGGGAAATGTTCTTCAATGGCCATGAAGGATTTTACAACATATATGTCCACCCTCATCCTTCATATGTTGATTCATGGCCTCAAAATTCTGTTTTCCATGGAAGGAGAATTCCAAGCAAGGCAGTTGAATGGGGAAGTCCTACAATGATCGATGGTGAGAGACGCCTACTAGCAAGTGCCCTCCTTGACTTTACTAACGAGAGATTTGTGCTACTATCCGAGTCTTGCATCCCATTGTTCAACTTCACCACAATATACAGCTACCTCATGAATGCCAGTGAGAGCAATATAGGATCATATGATGACCCGAGGAAGGTGGGTCGGGGCAGATACAATCCGAGAATGTGGCCTGAAATCAACATATCAGATTGGCGCAAAGGCTCCCAGTGGTTCGAGGTCAGCCGGAAGCTGGCAGTTGAGATCATATCAGACAACAAGTACTACCCCATATTCAAAGAGCATTGCAGCCCTCCTTGTTACATGGATGAGCATTACATCCCAACCCTAGTGAACATCATTGGCCCCGAAGAAAACTCGAATCGGAGCGTTACTTGGGTAGATTGGTCCAAAAGTGGTCCTCACCCTGGAAGATTTGGGAGGAATGATGTTTCAGAAGTAATTTTGAATCAGATAAGATTTGGTTCTAATTGCAGTTGTAGTGGTAATAGTAATGCAAATAGTACTATCACTTGTTCTATGTGTTTTTTGTTCGCTAGGAAGTTCATGGGAGACACTCTGCGGCCTCTCTTGCAAATTGCACCCATGTTGCTAGGTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

404

Amino Acids

45.79

Weight (kDa)

7.64

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 129 - 355 1.1e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AciI CCGC 4 cut(s) 188, 230, 233, 1171
AclI AACGTT 1 cut(s) 267
AclWI GGATC 1 cut(s) 733
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 2 cut(s) 517, 540
AcyI GRCGYC 1 cut(s) 595
AfaI GTAC 3 cut(s) 259, 872, 1114
AfiI CCNNNNNNNGG 7 cut(s) 247, 353, 432, 433, 745, 841, 1016
AgsI TTSAA 5 cut(s) 448, 560, 673, 886, 1057
AjnI CCWGG 1 cut(s) 1015
AluBI AGCT 3 cut(s) 40, 693, 841
AluI AGCT 3 cut(s) 40, 693, 841
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 2 cut(s) 586, 1155
Alw44I GTGCAC 1 cut(s) 296
AlwI GGATC 1 cut(s) 733
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 7 cut(s) 410, 423, 451, 509, 779, 952, 1172
ApaI GGGCCC 1 cut(s) 414
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 897
ApoI RAATTY 2 cut(s) 517, 540
AspLEI GCGC 1 cut(s) 807
AspS9I GGNCC 6 cut(s) 410, 411, 424, 953, 996, 1007
AsuHPI GGTGA 6 cut(s) 142, 219, 329, 599, 670, 1004
AsuII TTCGAA 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 2 cut(s) 996, 1007
BaeGI GKGCMC 3 cut(s) 300, 414, 613
BalI TGGCCA 1 cut(s) 453
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 1 cut(s) 909
BccI CCATC 3 cut(s) 298, 308, 578
BciT130I CCWGG 1 cut(s) 1017
BciVI GTATCC 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 586, 1155
BfaI CTAG 7 cut(s) 80, 381, 602, 938, 1146, 1202, 1210
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 4 cut(s) 188, 233, 898, 1172
BlsI GCNGC 4 cut(s) 189, 234, 899, 1173
BmcAI AGTACT 2 cut(s) 872, 1114
Bme1390I CCNGG 1 cut(s) 1017
Bme18I GGWCC 2 cut(s) 996, 1007
BmeT110I CYCGRG 1 cut(s) 739
BmgT120I GGNCC 6 cut(s) 410, 411, 424, 953, 996, 1007
BmiI GGNNCC 6 cut(s) 411, 412, 426, 814, 955, 1207
BmrFI CCNGG 1 cut(s) 1017
BmrI ACTGGG 1 cut(s) 811
BmsI GCATC 3 cut(s) 281, 344, 669
BmuI ACTGGG 1 cut(s) 811
BpmI CTGGAG 1 cut(s) 349
Bpu14I TTCGAA 1 cut(s) 35
Bsa29I ATCGAT 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 595
BsaJI CCNNGG 4 cut(s) 347, 529, 740, 1015
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 7 cut(s) 247, 353, 432, 433, 745, 841, 1016
Bse1I ACTGG 2 cut(s) 708, 817
Bse3DI GCAATG 1 cut(s) 892
BseBI CCWGG 1 cut(s) 1017
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 4 cut(s) 347, 529, 740, 1015
BseGI GGATG 4 cut(s) 487, 660, 922, 927
BseLI CCNNNNNNNGG 7 cut(s) 247, 353, 432, 433, 745, 841, 1016
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 2 cut(s) 38, 273
BseNI ACTGG 2 cut(s) 708, 817
BseRI GAGGAG 1 cut(s) 160
BseSI GKGCMC 3 cut(s) 300, 414, 613
BseXI GCAGC 1 cut(s) 909
BshFI GGCC 7 cut(s) 412, 425, 453, 511, 781, 954, 1174
BshVI ATCGAT 1 cut(s) 582
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 1 cut(s) 835
BslI CCNNNNNNNGG 7 cut(s) 247, 353, 432, 433, 745, 841, 1016
BsmAI GTCTC 2 cut(s) 586, 1155
BsmBI CGTCTC 1 cut(s) 586
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 7 cut(s) 412, 425, 453, 511, 781, 954, 1174
BsoBI CYCGRG 1 cut(s) 739
Bsp119I TTCGAA 1 cut(s) 35
Bsp120I GGGCCC 1 cut(s) 410
Bsp1286I GDGCHC 3 cut(s) 300, 414, 613
Bsp143I GATC 3 cut(s) 579, 725, 852
Bsp19I CCATGG 1 cut(s) 529
BspACI CCGC 4 cut(s) 188, 230, 233, 1171
BspANI GGCC 7 cut(s) 412, 425, 453, 511, 781, 954, 1174
BspCNI CTCAG 2 cut(s) 37, 274
BspDI ATCGAT 1 cut(s) 582
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 6 cut(s) 411, 412, 426, 814, 955, 1207
BspPI GGATC 1 cut(s) 733
BspT104I TTCGAA 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 892
BsrI ACTGG 2 cut(s) 708, 817
BssECI CCNNGG 4 cut(s) 347, 529, 740, 1015
BssMI GATC 3 cut(s) 579, 725, 852
BssNI GRCGYC 1 cut(s) 595
BssT1I CCWWGG 2 cut(s) 347, 529
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 595
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 192, 843
BstDEI CTNAG 2 cut(s) 24, 282
BstDSI CCRYGG 1 cut(s) 529
BstF5I GGATG 4 cut(s) 487, 660, 922, 927
BstHHI GCGC 1 cut(s) 807
BstKTI GATC 3 cut(s) 582, 728, 855
BstMAI GTCTC 2 cut(s) 586, 1155
BstMBI GATC 3 cut(s) 579, 725, 852
BstMWI GCNNNNNNNGC 2 cut(s) 187, 1180
BstNI CCWGG 1 cut(s) 1017
BstSCI CCNGG 1 cut(s) 1015
BstSLI GKGCMC 3 cut(s) 300, 414, 613
BstV1I GCAGC 1 cut(s) 909
BstXI CCANNNNNNTGG 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 582
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 7 cut(s) 412, 425, 453, 511, 781, 954, 1174
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 529
BtsCI GGATG 4 cut(s) 487, 660, 922, 927
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 3 cut(s) 176, 715, 824
Cac8I GCNNGC 2 cut(s) 192, 843
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 807
Cfr13I GGNCC 6 cut(s) 410, 411, 424, 953, 996, 1007
ClaI ATCGAT 1 cut(s) 582
CseI GACGC 1 cut(s) 603
Csp6I GTAC 3 cut(s) 258, 871, 1113
CviQI GTAC 3 cut(s) 258, 871, 1113
DdeI CTNAG 2 cut(s) 24, 282
DpnI GATC 3 cut(s) 581, 727, 854
DpnII GATC 3 cut(s) 579, 725, 852
EaeI YGGCCR 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 347, 529
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 996, 1007
Eco88I CYCGRG 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 1015
EcoT14I CCWWGG 2 cut(s) 347, 529
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 347, 529
Esp3I CGTCTC 1 cut(s) 586
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FauNDI CATATG 2 cut(s) 496, 730
Fnu4HI GCNGC 4 cut(s) 188, 233, 898, 1172
FokI GGATG 4 cut(s) 474, 647, 914, 929
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 4 cut(s) 188, 233, 898, 1172
FspBI CTAG 7 cut(s) 80, 381, 602, 938, 1146, 1202, 1210
GlaI GCGC 1 cut(s) 806
GluI GCNGC 4 cut(s) 188, 233, 898, 1172
GsuI CTGGAG 1 cut(s) 349
HaeIII GGCC 7 cut(s) 412, 425, 453, 511, 781, 954, 1174
HapII CCGG 1 cut(s) 835
HgaI GACGC 1 cut(s) 603
HhaI GCGC 1 cut(s) 807
Hin1I GRCGYC 1 cut(s) 595
Hin6I GCGC 1 cut(s) 805
HinP1I GCGC 1 cut(s) 805
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 7 cut(s) 111, 251, 384, 503, 653, 970, 1057
HpaII CCGG 1 cut(s) 835
HphI GGTGA 6 cut(s) 142, 219, 329, 599, 670, 1004
Hpy166II GTNNAC 3 cut(s) 298, 481, 943
Hpy188I TCNGA 9 cut(s) 17, 277, 652, 771, 799, 862, 975, 1045, 1062
Hpy188III TCNNGA 2 cut(s) 328, 700
Hpy8I GTNNAC 3 cut(s) 298, 481, 943
HpyAV CCTTC 6 cut(s) 148, 400, 452, 501, 528, 739
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 9 cut(s) 58, 272, 298, 660, 897, 1083, 1106, 1183, 1190
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 1180
HpyF3I CTNAG 2 cut(s) 24, 282
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 595
HspAI GCGC 1 cut(s) 805
Kzo9I GATC 3 cut(s) 579, 725, 852
LmnI GCTCC 2 cut(s) 818, 975
Lsp1109I GCAGC 1 cut(s) 909
LweI GCATC 3 cut(s) 281, 344, 669
MaeI CTAG 7 cut(s) 80, 381, 602, 938, 1146, 1202, 1210
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 3 cut(s) 174, 908, 979
MalI GATC 3 cut(s) 581, 727, 854
MboI GATC 3 cut(s) 579, 725, 852
MboII GAAGA 4 cut(s) 133, 436, 971, 1032
MhlI GDGCHC 3 cut(s) 300, 414, 613
MlsI TGGCCA 1 cut(s) 453
MluCI AATT 6 cut(s) 30, 517, 540, 1050, 1078, 1185
MluNI TGGCCA 1 cut(s) 453
MlyI GAGTC 2 cut(s) 120, 662
Mox20I TGGCCA 1 cut(s) 453
MscI TGGCCA 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 453
MspI CCGG 1 cut(s) 835
MspR9I CCNGG 1 cut(s) 1017
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 1 cut(s) 1017
MwoI GCNNNNNNNGC 2 cut(s) 187, 1180
NcoI CCATGG 1 cut(s) 529
NdeI CATATG 2 cut(s) 496, 730
NdeII GATC 3 cut(s) 579, 725, 852
NlaIV GGNNCC 6 cut(s) 411, 412, 426, 814, 955, 1207
NmuCI GTSAC 1 cut(s) 174
NspV TTCGAA 1 cut(s) 35
PagI TCATGA 1 cut(s) 699
PctI GAATGC 1 cut(s) 709
PfeI GAWTC 5 cut(s) 251, 384, 503, 970, 1057
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 4 cut(s) 189, 234, 899, 1173
PleI GAGTC 2 cut(s) 119, 661
PpsI GAGTC 2 cut(s) 119, 661
Psp1406I AACGTT 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 1015
PspGI CCWGG 1 cut(s) 1015
PspN4I GGNNCC 6 cut(s) 411, 412, 426, 814, 955, 1207
PspOMI GGGCCC 1 cut(s) 410
PspPI GGNCC 6 cut(s) 410, 411, 424, 953, 996, 1007
PstNI CAGNNNCTG 1 cut(s) 260
RsaI GTAC 3 cut(s) 259, 872, 1114
RsaNI GTAC 3 cut(s) 258, 871, 1113
SatI GCNGC 4 cut(s) 188, 233, 898, 1172
Sau3AI GATC 3 cut(s) 579, 725, 852
Sau96I GGNCC 6 cut(s) 410, 411, 424, 953, 996, 1007
ScaI AGTACT 2 cut(s) 872, 1114
SchI GAGTC 2 cut(s) 120, 662
ScrFI CCNGG 1 cut(s) 1017
SduI GDGCHC 3 cut(s) 300, 414, 613
SfaNI GCATC 3 cut(s) 281, 344, 669
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 2 cut(s) 996, 1007
Sse9I AATT 6 cut(s) 30, 517, 540, 1050, 1078, 1185
SsiI CCGC 4 cut(s) 188, 230, 233, 1171
SspMI CTAG 7 cut(s) 80, 381, 602, 938, 1146, 1202, 1210
StyD4I CCNGG 1 cut(s) 1015
StyI CCWWGG 2 cut(s) 347, 529
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 270
TaqI TCGA 4 cut(s) 35, 582, 825, 968
TasI AATT 6 cut(s) 30, 517, 540, 1050, 1078, 1185
TatI WGTACW 2 cut(s) 870, 1112
TauI GCSGC 3 cut(s) 190, 235, 1174
TfiI GAWTC 5 cut(s) 251, 384, 503, 970, 1057
TscAI CASTG 3 cut(s) 183, 715, 824
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 1 cut(s) 897
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 8 cut(s) 65, 114, 382, 471, 483, 495, 716, 1144
TspRI CASTG 3 cut(s) 183, 715, 824
Van91I CCANNNNNTGG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 2 cut(s) 996, 1007
XapI RAATTY 2 cut(s) 517, 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XspI CTAG 7 cut(s) 80, 381, 602, 938, 1146, 1202, 1210
ZrmI AGTACT 2 cut(s) 872, 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.