MD16G1039600.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004921
Physical Location & Seq
Reverse (-)
2811195 .. 2812681
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1039600.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGCTCAGAATCAGACCCAAAAAACAGAGAGCCATGAACCAAGGGAAGCTCACTGTAGTTGTCAAAGAGCTTACCACAGGCCATTTTCATGTCAAAATCACTATCATCCTCTCTGTGTCCATGCTTTTCTTCATTTTCTTTTTCGGAATCTTCGTTAACGATCACACGAAGAAATTCCTGAGCTCAGATCAGATCGTCCCTCAGCTCAACACATTCACCCCCCTCTCACCTCGGCCTTCCTACACACATCATTTCCAGTGCAACTTTTCTTTGTCCTCTCGCTTACTGCCCATTAGTATTTCAAGTCCAGGAGGGTCGACGTTGAAAGATTACTTAGCGCCCAAGGATCTATGGCATTCCATGAGTGACGAGGAGCTCTTCTGGCGAGCTTCGATGGTGCCACAAGTCATGGAGCACCCGTATAACCGAACACAAAAGGTGGCGTTTATGTTCCTAACAAAGGGACGATTGCCATTGGCGCCACTTTGGGAAATATTTTTCAGAGGACATGAAGGCTTATTCTCTGTTTATCTCCATTCAAGACCAGACTTTAAGATCGAGCCACCGGAGTCCTCGGTGTTTTACAAGCGCAGGATACCAAGCAAGATAGTTGAGTGGGGAAAGGCAACAATGGTTGATGCAGAGAGGCGCCTATTAGCCAATGCCTTACTCGATTTCTCAAACGAGAGATTCGTTTTGCTCTCTGAATCATGCATACCCCTCTTCAACTTCACTACAATCTACAAGTACCTCACCAACTCCAACCAAACTTTTGTTGGCTCATTCGACGATCCAAGACACATTGGTCGCGGGCGTTACAACCCGCAAATGTTTCCAACCGTGACGTTGTCAGACTGGCGGAAGGGCTCGCAATGGTTTGAAGTTCACCGGAAGCTTGCGGTGGAGGTAGTTTCCGACGTGACATACTACCCTAAATTTAAGGACCTTTGCCTGCCTCCTTGCTACACGGATGAGCACTACATGGCAACTCTTGTGACTAAAGTTGGCGCTGGCATGAATTCTAATAGGAGCATTACTTGGGTTGATTGGTCAAGAGGTGGCTCGCACCCTGCAACTTTTGTGAGGAAAGACGTGACAGAAAAGTTTCTCAATCGGTTAAGAGATGGATTTAATTGTACTTACAATGGTGGCGTGGGTTCCATTTGCCACCTCTTTGCAAGAAAGTTTCATCCAAGCACATTGGAGCCATTGCTAAGAATCGCTCCACGTCTATTTGGATTTAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

417

Amino Acids

48.12

Weight (kDa)

9.68

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 147 - 374 1.9e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 804
AccB1I GGYRCC 3 cut(s) 397, 478, 648
AccI GTMKAC 1 cut(s) 317
AccII CGCG 1 cut(s) 810
AciI CCGC 4 cut(s) 810, 824, 859, 899
AclWI GGATC 2 cut(s) 354, 785
AcsI RAATTY 3 cut(s) 173, 935, 1018
AcyI GRCGYC 2 cut(s) 479, 649
AfaI GTAC 2 cut(s) 749, 1138
AfiI CCNNNNNNNGG 1 cut(s) 460
AgsI TTSAA 5 cut(s) 303, 325, 540, 727, 881
AjiI CACGTC 3 cut(s) 919, 1093, 1229
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 7 cut(s) 49, 70, 183, 205, 376, 389, 895
AluI AGCT 7 cut(s) 49, 70, 183, 205, 376, 389, 895
Alw21I GWGCWC 4 cut(s) 185, 378, 417, 978
AlwI GGATC 2 cut(s) 354, 785
AoxI GGCC 2 cut(s) 79, 233
ApoI RAATTY 3 cut(s) 173, 935, 1018
Asp700I GAANNNNTTC 2 cut(s) 173, 1104
AspLEI GCGC 5 cut(s) 340, 481, 591, 651, 1010
AspS9I GGNCC 1 cut(s) 943
AsuHPI GGTGA 4 cut(s) 208, 219, 745, 878
AvaII GGWCC 1 cut(s) 943
BanI GGYRCC 3 cut(s) 397, 478, 648
BanII GRGCYC 3 cut(s) 185, 378, 869
Bbv12I GWGCWC 4 cut(s) 185, 378, 417, 978
BbvCI CCTCAGC 1 cut(s) 201
BccI CCATC 2 cut(s) 388, 1118
BciT130I CCWGG 1 cut(s) 309
BciVI GTATCC 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 54
BfoI RGCGCY 4 cut(s) 341, 482, 652, 1011
BfuI GTATCC 1 cut(s) 588
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 943
BmgBI CACGTC 3 cut(s) 919, 1093, 1229
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 5 cut(s) 399, 480, 650, 1159, 1206
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 628
Bpu10I CCTNAGC 2 cut(s) 179, 201
BsaHI GRCGYC 2 cut(s) 479, 649
BsaJI CCNNGG 4 cut(s) 40, 230, 342, 573
BsaWI WCCGGW 2 cut(s) 565, 888
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 256, 860
Bse3DI GCAATG 2 cut(s) 878, 1208
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 40, 230, 342, 573
BseGI GGATG 3 cut(s) 105, 976, 1189
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMI GCAATG 2 cut(s) 878, 1208
BseMII CTCAG 4 cut(s) 19, 170, 198, 215
BseNI ACTGG 2 cut(s) 256, 860
BseRI GAGGAG 1 cut(s) 386
Bsh1236I CGCG 1 cut(s) 810
BshFI GGCC 2 cut(s) 81, 235
BshNI GGYRCC 3 cut(s) 397, 478, 648
BsiHKAI GWGCWC 4 cut(s) 185, 378, 417, 978
BsiSI CCGG 2 cut(s) 566, 889
BslFI GGGAC 2 cut(s) 182, 477
BslI CCNNNNNNNGG 1 cut(s) 460
BsmFI GGGAC 2 cut(s) 182, 477
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 2 cut(s) 81, 235
Bsp1286I GDGCHC 5 cut(s) 185, 378, 417, 869, 978
Bsp143I GATC 6 cut(s) 160, 187, 192, 346, 555, 790
BspACI CCGC 4 cut(s) 810, 824, 859, 899
BspANI GGCC 2 cut(s) 81, 235
BspCNI CTCAG 4 cut(s) 18, 171, 197, 214
BspFNI CGCG 1 cut(s) 810
BspLI GGNNCC 5 cut(s) 399, 480, 650, 1159, 1206
BspPI GGATC 2 cut(s) 354, 785
BspQI GCTCTTC 1 cut(s) 383
BspT107I GGYRCC 3 cut(s) 397, 478, 648
BsrDI GCAATG 2 cut(s) 878, 1208
BsrI ACTGG 2 cut(s) 256, 860
BssECI CCNNGG 4 cut(s) 40, 230, 342, 573
BssMI GATC 6 cut(s) 160, 187, 192, 346, 555, 790
BssNI GRCGYC 2 cut(s) 479, 649
BssT1I CCWWGG 2 cut(s) 40, 342
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 55, 841
Bst6I CTCTTC 2 cut(s) 383, 728
BstACI GRCGYC 2 cut(s) 479, 649
BstC8I GCNNGC 7 cut(s) 387, 812, 869, 897, 953, 1012, 1064
BstDEI CTNAG 6 cut(s) 5, 179, 184, 201, 334, 1214
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 3 cut(s) 105, 976, 1189
BstFNI CGCG 1 cut(s) 810
BstH2I RGCGCY 4 cut(s) 341, 482, 652, 1011
BstHHI GCGC 5 cut(s) 340, 481, 591, 651, 1010
BstKTI GATC 6 cut(s) 163, 190, 195, 349, 558, 793
BstMBI GATC 6 cut(s) 160, 187, 192, 346, 555, 790
BstMWI GCNNNNNNNGC 2 cut(s) 382, 478
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 54
BstUI CGCG 1 cut(s) 810
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 2 cut(s) 81, 235
BtrI CACGTC 3 cut(s) 919, 1093, 1229
BtsCI GGATG 3 cut(s) 105, 976, 1189
BtsIMutI CAGTG 2 cut(s) 51, 263
Cac8I GCNNGC 7 cut(s) 387, 812, 869, 897, 953, 1012, 1064
CfoI GCGC 5 cut(s) 340, 481, 591, 651, 1010
Cfr13I GGNCC 1 cut(s) 943
Csp6I GTAC 2 cut(s) 748, 1137
CviAII CATG 9 cut(s) 34, 89, 121, 361, 409, 509, 711, 982, 1015
CviQI GTAC 2 cut(s) 748, 1137
DdeI CTNAG 6 cut(s) 5, 179, 184, 201, 334, 1214
DinI GGCGCC 2 cut(s) 480, 650
DpnI GATC 6 cut(s) 162, 189, 194, 348, 557, 792
DpnII GATC 6 cut(s) 160, 187, 192, 346, 555, 790
DrdI GACNNNNNNGTC 1 cut(s) 804
DseDI GACNNNNNNGTC 1 cut(s) 804
Eam1104I CTCTTC 2 cut(s) 383, 728
EarI CTCTTC 2 cut(s) 383, 728
EciI GGCGGA 1 cut(s) 874
Ecl136II GAGCTC 2 cut(s) 183, 376
Eco130I CCWWGG 2 cut(s) 40, 342
Eco24I GRGCYC 3 cut(s) 185, 378, 869
Eco47I GGWCC 1 cut(s) 943
Eco53kI GAGCTC 2 cut(s) 183, 376
EcoICRI GAGCTC 2 cut(s) 183, 376
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 943
EcoRI GAATTC 1 cut(s) 1018
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 40, 342
EcoT22I ATGCAT 1 cut(s) 716
EcoT38I GRGCYC 3 cut(s) 185, 378, 869
EgeI GGCGCC 2 cut(s) 480, 650
EheI GGCGCC 2 cut(s) 480, 650
ErhI CCWWGG 2 cut(s) 40, 342
FaeI CATG 9 cut(s) 37, 92, 124, 364, 412, 512, 714, 985, 1018
FaqI GGGAC 2 cut(s) 182, 477
FatI CATG 9 cut(s) 33, 88, 120, 360, 408, 508, 710, 981, 1014
FauI CCCGC 2 cut(s) 803, 831
FblI GTMKAC 1 cut(s) 317
FokI GGATG 3 cut(s) 92, 983, 1176
FriOI GRGCYC 3 cut(s) 185, 378, 869
GlaI GCGC 5 cut(s) 339, 480, 590, 650, 1009
HaeII RGCGCY 4 cut(s) 341, 482, 652, 1011
HaeIII GGCC 2 cut(s) 81, 235
HapII CCGG 2 cut(s) 566, 889
HhaI GCGC 5 cut(s) 340, 481, 591, 651, 1010
Hin1I GRCGYC 2 cut(s) 479, 649
Hin1II CATG 9 cut(s) 37, 92, 124, 364, 412, 512, 714, 985, 1018
Hin6I GCGC 5 cut(s) 338, 479, 589, 649, 1008
HinP1I GCGC 5 cut(s) 338, 479, 589, 649, 1008
HincII GTYRAC 2 cut(s) 157, 318
HindII GTYRAC 2 cut(s) 157, 318
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 6 cut(s) 9, 147, 569, 690, 707, 1218
HpaI GTTAAC 1 cut(s) 157
HpaII CCGG 2 cut(s) 566, 889
HphI GGTGA 4 cut(s) 208, 219, 745, 878
Hpy166II GTNNAC 3 cut(s) 157, 318, 886
Hpy188I TCNGA 9 cut(s) 8, 14, 146, 187, 192, 503, 706, 853, 916
Hpy188III TCNNGA 3 cut(s) 178, 540, 1053
Hpy8I GTNNAC 3 cut(s) 157, 318, 886
Hpy99I CGWCG 3 cut(s) 322, 791, 920
HpyAV CCTTC 3 cut(s) 246, 506, 856
HpyCH4III ACNGT 2 cut(s) 55, 841
HpyCH4IV ACGT 5 cut(s) 320, 845, 918, 1092, 1228
HpyCH4V TGCA 5 cut(s) 261, 641, 714, 1073, 1178
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 478
HpyF3I CTNAG 6 cut(s) 5, 179, 184, 201, 334, 1214
HpySE526I ACGT 5 cut(s) 320, 845, 918, 1092, 1228
Hsp92I GRCGYC 2 cut(s) 479, 649
Hsp92II CATG 9 cut(s) 37, 92, 124, 364, 412, 512, 714, 985, 1018
HspAI GCGC 5 cut(s) 338, 479, 589, 649, 1008
KasI GGCGCC 2 cut(s) 478, 648
KspAI GTTAAC 1 cut(s) 157
Kzo9I GATC 6 cut(s) 160, 187, 192, 346, 555, 790
LguI GCTCTTC 1 cut(s) 383
LmnI GCTCC 5 cut(s) 373, 412, 1029, 1204, 1228
LweI GCATC 1 cut(s) 628
MaeII ACGT 5 cut(s) 320, 845, 918, 1092, 1228
MaeIII GTNAC 6 cut(s) 365, 815, 841, 919, 994, 1093
MalI GATC 6 cut(s) 162, 189, 194, 348, 557, 792
MboI GATC 6 cut(s) 160, 187, 192, 346, 555, 790
MboII GAAGA 5 cut(s) 121, 142, 181, 370, 715
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 5 cut(s) 185, 378, 417, 869, 978
MluCI AATT 4 cut(s) 173, 935, 1018, 1132
Mly113I GGCGCC 2 cut(s) 479, 649
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 3 cut(s) 786, 860, 939
Mph1103I ATGCAT 1 cut(s) 716
MroXI GAANNNNTTC 2 cut(s) 173, 1104
MseI TTAA 6 cut(s) 156, 552, 939, 1118, 1131, 1242
MslI CAYNNNNRTG 1 cut(s) 87
MspI CCGG 2 cut(s) 566, 889
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 810
MwoI GCNNNNNNNGC 2 cut(s) 382, 478
NarI GGCGCC 2 cut(s) 479, 649
NdeII GATC 6 cut(s) 160, 187, 192, 346, 555, 790
NlaIII CATG 9 cut(s) 37, 92, 124, 364, 412, 512, 714, 985, 1018
NlaIV GGNNCC 5 cut(s) 399, 480, 650, 1159, 1206
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 5 cut(s) 365, 841, 919, 994, 1093
NsiI ATGCAT 1 cut(s) 716
PciSI GCTCTTC 1 cut(s) 383
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 690
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 2 cut(s) 173, 1104
PfeI GAWTC 5 cut(s) 9, 147, 690, 707, 1218
PflFI GACNNNGTC 1 cut(s) 847
PfoI TCCNGGA 1 cut(s) 307
PleI GAGTC 1 cut(s) 577
PluTI GGCGCC 2 cut(s) 482, 652
PpsI GAGTC 1 cut(s) 577
PpuMI RGGWCCY 1 cut(s) 943
Psp124BI GAGCTC 2 cut(s) 185, 378
Psp5II RGGWCCY 1 cut(s) 943
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 5 cut(s) 399, 480, 650, 1159, 1206
PspPI GGNCC 1 cut(s) 943
PspPPI RGGWCCY 1 cut(s) 943
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 847
RsaI GTAC 2 cut(s) 749, 1138
RsaNI GTAC 2 cut(s) 748, 1137
RseI CAYNNNNRTG 1 cut(s) 87
SacI GAGCTC 2 cut(s) 185, 378
SalI GTCGAC 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 6 cut(s) 156, 552, 939, 1118, 1131, 1242
Sau3AI GATC 6 cut(s) 160, 187, 192, 346, 555, 790
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 5 cut(s) 185, 378, 417, 869, 978
SfaNI GCATC 1 cut(s) 628
SfcI CTRYAG 1 cut(s) 54
SfoI GGCGCC 2 cut(s) 480, 650
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 4 cut(s) 173, 935, 1018, 1132
SsiI CCGC 4 cut(s) 810, 824, 859, 899
SspDI GGCGCC 2 cut(s) 478, 648
SspI AATATT 1 cut(s) 495
SstI GAGCTC 2 cut(s) 185, 378
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 40, 342
TaaI ACNGT 2 cut(s) 55, 841
TaiI ACGT 5 cut(s) 323, 848, 921, 1095, 1231
TaqI TCGA 5 cut(s) 317, 392, 558, 672, 786
TasI AATT 4 cut(s) 173, 935, 1018, 1132
TatI WGTACW 1 cut(s) 1136
TfiI GAWTC 5 cut(s) 9, 147, 690, 707, 1218
Tru1I TTAA 6 cut(s) 156, 552, 939, 1118, 1131, 1242
Tru9I TTAA 6 cut(s) 156, 552, 939, 1118, 1131, 1242
TscAI CASTG 2 cut(s) 58, 263
TseFI GTSAC 5 cut(s) 365, 841, 919, 994, 1093
Tsp45I GTSAC 5 cut(s) 365, 841, 919, 994, 1093
TspDTI ATGAA 6 cut(s) 50, 77, 121, 525, 1031, 1178
TspGWI ACGGA 1 cut(s) 983
TspRI CASTG 2 cut(s) 58, 263
Tth111I GACNNNGTC 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 943
XagI CCTNNNNNAGG 1 cut(s) 458
XapI RAATTY 3 cut(s) 173, 935, 1018
XcmI CCANNNNNNNNNTGG 1 cut(s) 773
XmiI GTMKAC 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 173, 1104
Zsp2I ATGCAT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.