FvH4_4g28590

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009555
Physical Location & Seq
Forward (+)
29190528 .. 29193807
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28590.t1

Sequence Viewer

Length: 873 bp
ATGATTCCACTGCAAATTCACCACCTCACTCCACCCTACTTGCCAATTTCCCAAACTACCCCTCATCTCCTCTCTCCCTTCTCCCAAAGACTCCAACTTTCCTCCAAACCCAACCTCCTCACCTTCCCCCCAATTCCACTCCCAAAACCCACCTCCAAGCTTTTAAGGGCTTCACAAAGTCAGTCTCTTTTCTTCGGAAATGAGGAGGACACCACCGGAATTTCATCTGGTCTCGACGACTTGTCCCCAAACGGCGTCGTTTACCGGAAAACACTTGCATTGGTTGAGTGCTCCATGTTTGCTGCTCTTACTGGGTTGGTCTATTTCCTCAGCAATTCTCTCGGAATTGAGAATTACTTTGGTTGCTTCTTTTCGCTGCCAATAGTGATCACCTCCATGAGATGGGGCGTCGCAGCTGGAAGGAAAACTATGGTGGCAACGACTATGCTGTTACTTGTCTTGTCTGGTCCGCTGAAAGCCTTAACCTATCTGCTTAAGCATGGACTAGTTGGCTTCACAATGGGAAGTTTGTGGAGGTCGGGAGCAAATTGGACTACTTCCGTTTTGTTGTGTGCAATTGTTCGAGCATTGGGTTCTGTAGGCTATGTTTTGATCACTTCTTTCTTGATAAGAGAAAACATATTTGCTCTGATCACCATAAACATTCATGCTTCTCTAACATTCATCTTCACAGCAGCGGGCATCTATACAGTTCCATCGATGGAGGCGATATATGCCTTGTTTAGTATTCTGGTTTTTATAAATAGCGGTTCCTTCACGTTCTTGTTGCACCTATTGTATGCTGCCTTCCTTTCGAGGCTCGGGATGAAGGCATCTTTAAGATTGCCAAGATGGCTGGAGAAGGCGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

291

Amino Acids

31.81

Weight (kDa)

9.74

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 121 - 277 5.5e-08 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AccB7I CCANNNNNTGG 1 cut(s) 402
AciI CCGC 3 cut(s) 470, 698, 768
AcsI RAATTY 2 cut(s) 15, 219
AcyI GRCGYC 2 cut(s) 255, 408
AfiI CCNNNNNNNGG 1 cut(s) 402
AflII CTTAAG 1 cut(s) 494
AhdI GACNNNNNGTC 1 cut(s) 241
AhlI ACTAGT 1 cut(s) 505
AluBI AGCT 2 cut(s) 160, 416
AluI AGCT 2 cut(s) 160, 416
Alw21I GWGCWC 1 cut(s) 293
Alw26I GTCTC 2 cut(s) 189, 236
Ama87I CYCGRG 1 cut(s) 821
ApeKI GCWGC 5 cut(s) 302, 376, 413, 695, 803
ApoI RAATTY 2 cut(s) 15, 219
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 4 cut(s) 11, 112, 382, 646
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 1 cut(s) 467
BarI GAAGNNNNNNTAC 2 cut(s) 791, 823
Bbv12I GWGCWC 1 cut(s) 293
BbvCI CCTCAGC 1 cut(s) 329
BbvI GCAGC 5 cut(s) 289, 363, 425, 707, 790
BccI CCATC 4 cut(s) 396, 715, 724, 846
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BclI TGATCA 3 cut(s) 387, 612, 651
BcoDI GTCTC 2 cut(s) 189, 236
BcuI ACTAGT 1 cut(s) 505
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 494
BglI GCCNNNNNGGC 1 cut(s) 853
BisI GCNGC 5 cut(s) 303, 377, 414, 696, 804
BlsI GCNGC 5 cut(s) 304, 378, 415, 697, 805
Bme18I GGWCC 1 cut(s) 467
BmeRI GACNNNNNGTC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 1 cut(s) 772
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 711, 842
BmuI ACTGGG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 329
Bsa29I ATCGAT 1 cut(s) 719
BsaHI GRCGYC 2 cut(s) 255, 408
BsaI GGTCTC 1 cut(s) 236
BsaWI WCCGGW 2 cut(s) 215, 264
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 316
BseCI ATCGAT 1 cut(s) 719
BseGI GGATG 1 cut(s) 831
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMII CTCAG 1 cut(s) 343
BseNI ACTGG 1 cut(s) 316
BseRI GAGGAG 3 cut(s) 59, 107, 218
BseXI GCAGC 5 cut(s) 289, 363, 425, 707, 790
BshVI ATCGAT 1 cut(s) 719
BsiHKAI GWGCWC 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 2 cut(s) 216, 265
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 2 cut(s) 189, 236
BsmFI GGGAC 1 cut(s) 229
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 1 cut(s) 293
Bsp143I GATC 3 cut(s) 387, 612, 651
BspACI CCGC 3 cut(s) 470, 698, 768
BspCNI CTCAG 1 cut(s) 342
BspDI ATCGAT 1 cut(s) 719
BspLI GGNNCC 1 cut(s) 772
BspTI CTTAAG 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 236
BsrI ACTGG 1 cut(s) 316
BssMI GATC 3 cut(s) 387, 612, 651
BssNI GRCGYC 2 cut(s) 255, 408
Bst4CI ACNGT 1 cut(s) 712
BstACI GRCGYC 2 cut(s) 255, 408
BstAFI CTTAAG 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 700
BstDEI CTNAG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 831
BstKTI GATC 3 cut(s) 390, 615, 654
BstMAI GTCTC 2 cut(s) 189, 236
BstMBI GATC 3 cut(s) 387, 612, 651
BstMWI GCNNNNNNNGC 2 cut(s) 734, 853
BstSFI CTRYAG 1 cut(s) 597
BstV1I GCAGC 5 cut(s) 289, 363, 425, 707, 790
Bsu15I ATCGAT 1 cut(s) 719
BsuTUI ATCGAT 1 cut(s) 719
BtsCI GGATG 1 cut(s) 831
BtsI GCAGTG 1 cut(s) 8
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 1 cut(s) 700
Cfr13I GGNCC 1 cut(s) 467
ClaI ATCGAT 1 cut(s) 719
CseI GACGC 2 cut(s) 244, 397
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
CviAII CATG 4 cut(s) 295, 397, 500, 668
CviJI RGCY 8 cut(s) 160, 170, 416, 479, 513, 603, 820, 856
CviKI_1 RGCY 8 cut(s) 160, 170, 416, 479, 513, 603, 820, 856
DdeI CTNAG 1 cut(s) 329
DpnI GATC 3 cut(s) 389, 614, 653
DpnII GATC 3 cut(s) 387, 612, 651
DriI GACNNNNNGTC 1 cut(s) 241
Eam1105I GACNNNNNGTC 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 821
FaeI CATG 4 cut(s) 298, 400, 503, 671
FaqI GGGAC 1 cut(s) 229
FatI CATG 4 cut(s) 294, 396, 499, 667
FauI CCCGC 1 cut(s) 691
FbaI TGATCA 3 cut(s) 387, 612, 651
Fnu4HI GCNGC 5 cut(s) 303, 377, 414, 696, 804
FokI GGATG 1 cut(s) 838
Fsp4HI GCNGC 5 cut(s) 303, 377, 414, 696, 804
FspBI CTAG 1 cut(s) 506
GluI GCNGC 5 cut(s) 303, 377, 414, 696, 804
HapII CCGG 2 cut(s) 216, 265
HgaI GACGC 2 cut(s) 244, 397
Hin1I GRCGYC 2 cut(s) 255, 408
Hin1II CATG 4 cut(s) 298, 400, 503, 671
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 4, 90
HpaII CCGG 2 cut(s) 216, 265
HphI GGTGA 4 cut(s) 11, 112, 382, 646
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 3 cut(s) 197, 344, 651
Hpy188III TCNNGA 4 cut(s) 233, 540, 625, 823
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 3 cut(s) 239, 260, 413
HpyAV CCTTC 7 cut(s) 88, 133, 414, 784, 817, 823, 856
HpyCH4III ACNGT 1 cut(s) 712
HpyCH4IV ACGT 1 cut(s) 779
HpyCH4V TGCA 4 cut(s) 13, 278, 575, 790
HpyF10VI GCNNNNNNNGC 2 cut(s) 734, 853
HpyF3I CTNAG 1 cut(s) 329
HpySE526I ACGT 1 cut(s) 779
Hsp92I GRCGYC 2 cut(s) 255, 408
Hsp92II CATG 4 cut(s) 298, 400, 503, 671
Ksp22I TGATCA 3 cut(s) 387, 612, 651
Kzo9I GATC 3 cut(s) 387, 612, 651
LmnI GCTCC 2 cut(s) 296, 542
LpnPI CCDG 8 cut(s) 213, 229, 278, 297, 402, 450, 737, 842
Lsp1109I GCAGC 5 cut(s) 289, 363, 425, 707, 790
LweI GCATC 2 cut(s) 711, 842
MaeI CTAG 1 cut(s) 506
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 1 cut(s) 450
MalI GATC 3 cut(s) 389, 614, 653
MboI GATC 3 cut(s) 387, 612, 651
MboII GAAGA 2 cut(s) 184, 679
MfeI CAATTG 1 cut(s) 576
MhlI GDGCHC 1 cut(s) 293
MluCI AATT 9 cut(s) 15, 45, 132, 219, 334, 345, 352, 547, 576
MlyI GAGTC 1 cut(s) 84
MmeI TCCRAC 1 cut(s) 118
MseI TTAA 4 cut(s) 164, 482, 495, 839
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 3 cut(s) 416, 472, 698
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 2 cut(s) 216, 265
MunI CAATTG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 734, 853
NdeII GATC 3 cut(s) 387, 612, 651
NlaIII CATG 4 cut(s) 298, 400, 503, 671
NlaIV GGNNCC 1 cut(s) 772
PcsI WCGNNNNNNNCGW 1 cut(s) 725
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 402
PkrI GCNGC 5 cut(s) 304, 378, 415, 697, 805
PleI GAGTC 1 cut(s) 84
PpsI GAGTC 1 cut(s) 84
PsiI TTATAA 1 cut(s) 761
PspN4I GGNNCC 1 cut(s) 772
PspPI GGNCC 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 416
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 4 cut(s) 164, 482, 495, 839
SatI GCNGC 5 cut(s) 303, 377, 414, 696, 804
Sau3AI GATC 3 cut(s) 387, 612, 651
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 1 cut(s) 84
SduI GDGCHC 1 cut(s) 293
SfaNI GCATC 2 cut(s) 711, 842
SfcI CTRYAG 1 cut(s) 597
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SpeI ACTAGT 1 cut(s) 505
Sse9I AATT 9 cut(s) 15, 45, 132, 219, 334, 345, 352, 547, 576
SsiI CCGC 3 cut(s) 470, 698, 768
SspMI CTAG 1 cut(s) 506
TaaI ACNGT 1 cut(s) 712
TaiI ACGT 1 cut(s) 782
TaqI TCGA 4 cut(s) 234, 583, 719, 815
TasI AATT 9 cut(s) 15, 45, 132, 219, 334, 345, 352, 547, 576
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 4 cut(s) 164, 482, 495, 839
Tru9I TTAA 4 cut(s) 164, 482, 495, 839
TscAI CASTG 1 cut(s) 15
TseI GCWGC 5 cut(s) 302, 376, 413, 695, 803
TspDTI ATGAA 4 cut(s) 213, 656, 673, 842
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 1 cut(s) 15
Van91I CCANNNNNTGG 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 2 cut(s) 15, 219
XspI CTAG 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.