MD16G1057600.v1.1

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009555
Physical Location & Seq
Reverse (-)
4118648 .. 4120498
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1057600.v1.1.491

Sequence Viewer

Length: 693 bp
ATGATTCCTCTGCAAGTCCACCACCTCCCCCCACCTTTCCTCATTTCCCAAACTACCCCTCTCCACTCTCTCTCTCCCACCTCTCAAACTGTCAAATTTTCCCCCAAGCCGAACCCCTTTTCGTCTTCCCCAATTTTGCTGTCCAACCCCCCCTGCAAATTTCTCAGGGCAGCAGGAATCCAAACGTCGTCTCTTTGTAGAAATGAGGAAGAAAGCACTGAAATTTCGACCGAGCTCGAAGACTTGTCGCCGGACGGCGTCGTTTATAAGAAGACGTTAGCGTTGGTGGAGTGCTCCATGTTTGCTGCTCTCACTGGCCTGGTGTACTTCCTCAGCAATTCTCTCGCAATTGAGGATTACTTTGGCTGCTTCTTCTCGCTGCCGGTAGTGATCACCTCAATGAGGTGGGGCATCGCTACTGGAAGAAAAACTATGGTTGCAACAACTATGCTATTGCTAGTCTTGTCTGGTCCGGTGAAAGCCTTAACCTATTTGCTTAAGCACGGTTTAGTTGGTTTTACAATGGGAAGTTTATGGAGGTCAGGAGCAAACTGGAGTCTTTCAATTTTCTTGTGCACAATTGTTCGATCACTGGGTGCTGTAGGGTATGTCTTGATATCGTCATTCTTGATAAGTGAAAACATACTTGCTTTGGTAATTAACTATCCTTTGTTTGAAATTCGTTTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

231

Amino Acids

25.21

Weight (kDa)

8.28

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 123 - 221 5e-08 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AcsI RAATTY 4 cut(s) 95, 158, 222, 678
AcyI GRCGYC 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 596
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 402
AflII CTTAAG 1 cut(s) 497
AgsI TTSAA 2 cut(s) 564, 677
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
Alw21I GWGCWC 3 cut(s) 237, 296, 578
Alw26I GTCTC 1 cut(s) 195
Alw44I GTGCAC 1 cut(s) 574
AoxI GGCC 1 cut(s) 316
ApaLI GTGCAC 1 cut(s) 574
ApeKI GCWGC 4 cut(s) 170, 305, 366, 379
ApoI RAATTY 4 cut(s) 95, 158, 222, 678
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 385, 487
AvaII GGWCC 1 cut(s) 470
BaeGI GKGCMC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 3 cut(s) 117, 246, 278
Bbv12I GWGCWC 3 cut(s) 237, 296, 578
BbvCI CCTCAGC 1 cut(s) 332
BbvI GCAGC 4 cut(s) 182, 292, 353, 366
BceAI ACGGC 1 cut(s) 271
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BciT130I CCWGG 1 cut(s) 320
BclI TGATCA 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 600
BfrI CTTAAG 1 cut(s) 497
BisI GCNGC 4 cut(s) 171, 306, 367, 380
BlsI GCNGC 4 cut(s) 172, 307, 368, 381
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmrFI CCNGG 1 cut(s) 320
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 420
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 3 cut(s) 117, 246, 278
BpmI CTGGAG 1 cut(s) 574
Bpu10I CCTNAGC 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 258
BsaWI WCCGGW 1 cut(s) 472
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 4 cut(s) 319, 424, 557, 597
BseBI CCWGG 1 cut(s) 320
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMII CTCAG 2 cut(s) 178, 346
BseNI ACTGG 4 cut(s) 319, 424, 557, 597
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 4 cut(s) 182, 292, 353, 366
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 318
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 3 cut(s) 237, 296, 578
BsiSI CCGG 3 cut(s) 251, 383, 473
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 1 cut(s) 195
BsmBI CGTCTC 1 cut(s) 195
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 237, 296, 578
Bsp143I GATC 2 cut(s) 390, 587
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 2 cut(s) 177, 345
BspTI CTTAAG 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 4 cut(s) 319, 424, 557, 597
BssAI RCCGGY 1 cut(s) 382
BssMI GATC 2 cut(s) 390, 587
BssNI GRCGYC 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 91, 506
BstACI GRCGYC 1 cut(s) 258
BstAFI CTTAAG 1 cut(s) 497
BstDEI CTNAG 2 cut(s) 164, 332
BstENI CCTNNNNNAGG 1 cut(s) 400
BstKTI GATC 2 cut(s) 393, 590
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 2 cut(s) 390, 587
BstMCI CGRYCG 1 cut(s) 231
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 600
BstSLI GKGCMC 1 cut(s) 578
BstV1I GCAGC 4 cut(s) 182, 292, 353, 366
BstV2I GAAGAC 3 cut(s) 117, 246, 278
BsuRI GGCC 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 397
BtsIMutI CAGTG 3 cut(s) 216, 312, 590
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 470
CseI GACGC 1 cut(s) 247
Csp6I GTAC 1 cut(s) 325
CviAII CATG 1 cut(s) 298
CviJI RGCY 5 cut(s) 109, 235, 318, 366, 482
CviKI_1 RGCY 5 cut(s) 109, 235, 318, 366, 482
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 2 cut(s) 164, 332
DpnI GATC 2 cut(s) 392, 589
DpnII GATC 2 cut(s) 390, 587
DraIII CACNNNGTG 1 cut(s) 596
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 318
EcoRV GATATC 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 237
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 1 cut(s) 301
FaiI YATR 8 cut(s) 267, 299, 434, 449, 535, 609, 644, 689
FatI CATG 1 cut(s) 297
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 4 cut(s) 171, 306, 367, 380
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 4 cut(s) 171, 306, 367, 380
FspBI CTAG 1 cut(s) 458
GluI GCNGC 4 cut(s) 171, 306, 367, 380
GsuI CTGGAG 1 cut(s) 574
HaeIII GGCC 1 cut(s) 318
HapII CCGG 3 cut(s) 251, 383, 473
HgaI GACGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 1 cut(s) 301
HinfI GANTC 3 cut(s) 4, 177, 556
HpaII CCGG 3 cut(s) 251, 383, 473
HphI GGTGA 2 cut(s) 385, 487
Hpy166II GTNNAC 3 cut(s) 19, 325, 576
Hpy188III TCNNGA 3 cut(s) 543, 613, 628
Hpy8I GTNNAC 3 cut(s) 19, 325, 576
Hpy99I CGWCG 2 cut(s) 190, 263
HpyCH4III ACNGT 2 cut(s) 91, 506
HpyCH4IV ACGT 2 cut(s) 185, 275
HpyCH4V TGCA 4 cut(s) 13, 156, 440, 576
HpyF3I CTNAG 2 cut(s) 164, 332
HpySE526I ACGT 2 cut(s) 185, 275
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 2 cut(s) 390, 587
LmnI GCTCC 2 cut(s) 299, 545
Lsp1109I GCAGC 4 cut(s) 182, 292, 353, 366
LweI GCATC 1 cut(s) 420
MaeI CTAG 1 cut(s) 458
MaeII ACGT 2 cut(s) 185, 275
MalI GATC 2 cut(s) 392, 589
MboI GATC 2 cut(s) 390, 587
MboII GAAGA 6 cut(s) 117, 221, 251, 283, 364, 435
MfeI CAATTG 2 cut(s) 348, 579
MhlI GDGCHC 3 cut(s) 237, 296, 578
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 1 cut(s) 168
MseI TTAA 3 cut(s) 485, 498, 660
MslI CAYNNNNRTG 1 cut(s) 398
MspCI CTTAAG 1 cut(s) 497
MspI CCGG 3 cut(s) 251, 383, 473
MspR9I CCNGG 1 cut(s) 320
MunI CAATTG 2 cut(s) 348, 579
MvaI CCWGG 1 cut(s) 320
NdeII GATC 2 cut(s) 390, 587
NlaIII CATG 1 cut(s) 301
PfeI GAWTC 2 cut(s) 4, 177
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 4 cut(s) 172, 307, 368, 381
PleI GAGTC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 564
PsiI TTATAA 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspPI GGNCC 1 cut(s) 470
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 3 cut(s) 485, 498, 660
SatI GCNGC 4 cut(s) 171, 306, 367, 380
Sau3AI GATC 2 cut(s) 390, 587
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 3 cut(s) 237, 296, 578
SfaNI GCATC 1 cut(s) 420
SfcI CTRYAG 1 cut(s) 600
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 398
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 91, 506
TaiI ACGT 2 cut(s) 188, 278
TaqI TCGA 3 cut(s) 227, 237, 586
TaqII GACCGA 1 cut(s) 245
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 2 cut(s) 4, 177
Tru1I TTAA 3 cut(s) 485, 498, 660
Tru9I TTAA 3 cut(s) 485, 498, 660
TscAI CASTG 3 cut(s) 223, 319, 597
TseI GCWGC 4 cut(s) 170, 305, 366, 379
TspRI CASTG 3 cut(s) 223, 319, 597
Tth111I GACNNNGTC 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 497
VneI GTGCAC 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 400
XapI RAATTY 4 cut(s) 95, 158, 222, 678
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.