RchiOBHm_Chr4g0436681

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009555
Physical Location & Seq
Forward (+)
59921038 .. 59923459
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40500

Sequence Viewer

Length: 882 bp
ATGATTCCTCTGCAAATTCAGCACCTCACTCCACCCTTGTCAATTTCCCAAACTACCCCTCTCTCCTCTCTCCCTTCCCTTCCTCACCTCCTCTCTCAAAGACTCGACCTTTCCCTCAGACCCAACCCCTTCACCTTCTCCCCAATTTCACCGCCCAAACCCACCCTCAAGCTCTCAAGGGCGTCGCAAAGTCGATCGCTTTTCTTCGGAAATGAGGACACCGCCGGAATTTCAACTGACCTCGACGACTTGTCCCCAAACGGCGTCGTTCACCGGAAAACATTGGCATTGGTTGAGTGCTCCATGTTTGCTGCGCTTACTGGGTTGGTGTATTTCCTCAGCAATTCTCTCGCAATTGAGAATTACTTTGGTTGCTTCTTTTCGCTGCCCATAGTGATCACCTCAATGAGATGGGGCACCGCAGCTGGAAGAAAAACTATGGTGGCAACGACTATGCTATTACTTGTCTTGTCTGGTCCGGTGAAAGCCTTAACCTATTTGCTTAAGCATGGATTAGTTGGCTTTACAATGGGAAGTTTGTGGAGGTCGGGAGCAAATTGGACTATTTCAGTTTTCTTGTGTGCAATTGTTCGAGCATTGGGTTCTGTAGGGTATGTTTTGATCACTTCTTTCTTGATCAGAGAAAACATATTTGCTCTGATCACGATAAACATTCATGCTTCTCTAACATTCATCTTCACAGCAGCAGGCATCTATACAGTTCCATCGATGGAAGCAATATATGCCTTATTTAGTATTCTGGTTTTTATAAATAGTGGGTCCTTCACATTCTTGCTGCACATACTGTATGGTGCCTTCCTTTCGAGGCTCGGGATGAAAGCATCTTTGAGATTGCCAAGATGGCTGGAGAAGGCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

293

Amino Acids

32.07

Weight (kDa)

9.86

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 125 - 280 1.8e-07 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 770
AccB1I GGYRCC 2 cut(s) 416, 812
AciI CCGC 3 cut(s) 152, 222, 420
AcsI RAATTY 2 cut(s) 15, 228
AcyI GRCGYC 2 cut(s) 182, 264
AflII CTTAAG 1 cut(s) 503
AgsI TTSAA 1 cut(s) 234
AhdI GACNNNNNGTC 1 cut(s) 250
AluBI AGCT 2 cut(s) 172, 425
AluI AGCT 2 cut(s) 172, 425
Alw21I GWGCWC 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 830
ApeKI GCWGC 5 cut(s) 311, 385, 422, 704, 796
ApoI RAATTY 2 cut(s) 15, 228
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 2 cut(s) 476, 780
AsuHPI GGTGA 6 cut(s) 77, 124, 141, 263, 391, 493
AvaI CYCGRG 1 cut(s) 830
AvaII GGWCC 2 cut(s) 476, 780
BaeGI GKGCMC 1 cut(s) 419
BanI GGYRCC 2 cut(s) 416, 812
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
Bbv12I GWGCWC 1 cut(s) 302
BbvCI CCTCAGC 1 cut(s) 338
BbvI GCAGC 5 cut(s) 298, 372, 434, 716, 783
BccI CCATC 4 cut(s) 405, 724, 733, 855
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BclI TGATCA 4 cut(s) 396, 621, 636, 660
BfmI CTRYAG 1 cut(s) 606
BfrI CTTAAG 1 cut(s) 503
BglI GCCNNNNNGGC 1 cut(s) 862
BisI GCNGC 5 cut(s) 312, 386, 423, 705, 797
BlsI GCNGC 5 cut(s) 313, 387, 424, 706, 798
Bme18I GGWCC 2 cut(s) 476, 780
BmeRI GACNNNNNGTC 1 cut(s) 250
BmeT110I CYCGRG 1 cut(s) 830
BmgT120I GGNCC 2 cut(s) 476, 780
BmiI GGNNCC 3 cut(s) 418, 781, 814
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 720, 851
BmuI ACTGGG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 2 cut(s) 152, 160
Bsa29I ATCGAT 1 cut(s) 728
BsaHI GRCGYC 2 cut(s) 182, 264
BsaWI WCCGGW 2 cut(s) 273, 478
Bse1I ACTGG 1 cut(s) 325
BseCI ATCGAT 1 cut(s) 728
BseGI GGATG 1 cut(s) 840
BseMII CTCAG 2 cut(s) 130, 352
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 55, 80
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 5 cut(s) 298, 372, 434, 716, 783
BsgI GTGCAG 1 cut(s) 782
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 2 cut(s) 416, 812
BshVI ATCGAT 1 cut(s) 728
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 3 cut(s) 225, 274, 479
BslFI GGGAC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 830
Bsp1286I GDGCHC 2 cut(s) 302, 419
Bsp143I GATC 5 cut(s) 194, 396, 621, 636, 660
BspACI CCGC 3 cut(s) 152, 222, 420
BspCNI CTCAG 2 cut(s) 129, 351
BspDI ATCGAT 1 cut(s) 728
BspLI GGNNCC 3 cut(s) 418, 781, 814
BspT107I GGYRCC 2 cut(s) 416, 812
BspTI CTTAAG 1 cut(s) 503
BsrI ACTGG 1 cut(s) 325
BssMI GATC 5 cut(s) 194, 396, 621, 636, 660
BssNI GRCGYC 2 cut(s) 182, 264
Bst4CI ACNGT 2 cut(s) 721, 807
BstACI GRCGYC 2 cut(s) 182, 264
BstAFI CTTAAG 1 cut(s) 503
BstAPI GCANNNNNTGC 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 709
BstDEI CTNAG 2 cut(s) 116, 338
BstF5I GGATG 1 cut(s) 840
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 5 cut(s) 197, 399, 624, 639, 663
BstMBI GATC 5 cut(s) 194, 396, 621, 636, 660
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 3 cut(s) 19, 743, 862
BstSFI CTRYAG 1 cut(s) 606
BstSLI GKGCMC 1 cut(s) 419
BstV1I GCAGC 5 cut(s) 298, 372, 434, 716, 783
Bsu15I ATCGAT 1 cut(s) 728
BsuTUI ATCGAT 1 cut(s) 728
BtsCI GGATG 1 cut(s) 840
Cac8I GCNNGC 1 cut(s) 709
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 476, 780
ClaI ATCGAT 1 cut(s) 728
CseI GACGC 2 cut(s) 171, 253
CviAII CATG 3 cut(s) 304, 509, 677
CviJI RGCY 6 cut(s) 172, 425, 488, 522, 829, 865
CviKI_1 RGCY 6 cut(s) 172, 425, 488, 522, 829, 865
DdeI CTNAG 2 cut(s) 116, 338
DpnI GATC 5 cut(s) 196, 398, 623, 638, 662
DpnII GATC 5 cut(s) 194, 396, 621, 636, 660
DriI GACNNNNNGTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 476, 780
Eco88I CYCGRG 1 cut(s) 830
EcoO109I RGGNCCY 1 cut(s) 780
FaeI CATG 3 cut(s) 307, 512, 680
FaqI GGGAC 1 cut(s) 238
FatI CATG 3 cut(s) 303, 508, 676
FbaI TGATCA 4 cut(s) 396, 621, 636, 660
Fnu4HI GCNGC 5 cut(s) 312, 386, 423, 705, 797
FokI GGATG 1 cut(s) 847
Fsp4HI GCNGC 5 cut(s) 312, 386, 423, 705, 797
GlaI GCGC 1 cut(s) 315
GluI GCNGC 5 cut(s) 312, 386, 423, 705, 797
HapII CCGG 3 cut(s) 225, 274, 479
HgaI GACGC 2 cut(s) 171, 253
HhaI GCGC 1 cut(s) 316
Hin1I GRCGYC 2 cut(s) 182, 264
Hin1II CATG 3 cut(s) 307, 512, 680
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HinfI GANTC 2 cut(s) 4, 102
HpaII CCGG 3 cut(s) 225, 274, 479
HphI GGTGA 6 cut(s) 77, 124, 141, 263, 391, 493
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 4 cut(s) 119, 209, 641, 660
Hpy188III TCNNGA 4 cut(s) 549, 634, 664, 832
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 3 cut(s) 187, 248, 269
HpyAV CCTTC 7 cut(s) 84, 89, 139, 145, 793, 826, 865
HpyCH4III ACNGT 2 cut(s) 721, 807
HpyCH4V TGCA 3 cut(s) 13, 584, 799
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 743, 862
HpyF3I CTNAG 2 cut(s) 116, 338
Hsp92I GRCGYC 2 cut(s) 182, 264
Hsp92II CATG 3 cut(s) 307, 512, 680
HspAI GCGC 1 cut(s) 314
Ksp22I TGATCA 4 cut(s) 396, 621, 636, 660
Kzo9I GATC 5 cut(s) 194, 396, 621, 636, 660
LmnI GCTCC 2 cut(s) 305, 551
LpnPI CCDG 9 cut(s) 238, 287, 306, 411, 459, 492, 693, 746, 851
Lsp1109I GCAGC 5 cut(s) 298, 372, 434, 716, 783
LweI GCATC 2 cut(s) 720, 851
MalI GATC 5 cut(s) 196, 398, 623, 638, 662
MboI GATC 5 cut(s) 194, 396, 621, 636, 660
MboII GAAGA 3 cut(s) 196, 441, 688
MfeI CAATTG 2 cut(s) 354, 585
MhlI GDGCHC 2 cut(s) 302, 419
MluCI AATT 9 cut(s) 15, 42, 144, 228, 343, 354, 361, 556, 585
MlyI GAGTC 1 cut(s) 96
MseI TTAA 2 cut(s) 491, 504
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 425
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 3 cut(s) 225, 274, 479
MunI CAATTG 2 cut(s) 354, 585
MwoI GCNNNNNNNGC 3 cut(s) 19, 743, 862
NdeII GATC 5 cut(s) 194, 396, 621, 636, 660
NlaIII CATG 3 cut(s) 307, 512, 680
NlaIV GGNNCC 3 cut(s) 418, 781, 814
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 5 cut(s) 313, 387, 424, 706, 798
Ple19I CGATCG 1 cut(s) 197
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 780
PsiI TTATAA 1 cut(s) 770
Psp5II RGGWCCY 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 418, 781, 814
PspPI GGNCC 2 cut(s) 476, 780
PspPPI RGGWCCY 1 cut(s) 780
PvuI CGATCG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 425
RseI CAYNNNNRTG 1 cut(s) 404
SaqAI TTAA 2 cut(s) 491, 504
SatI GCNGC 5 cut(s) 312, 386, 423, 705, 797
Sau3AI GATC 5 cut(s) 194, 396, 621, 636, 660
Sau96I GGNCC 2 cut(s) 476, 780
SchI GAGTC 1 cut(s) 96
SduI GDGCHC 2 cut(s) 302, 419
SfaNI GCATC 2 cut(s) 720, 851
SfcI CTRYAG 1 cut(s) 606
SinI GGWCC 2 cut(s) 476, 780
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 3 cut(s) 167, 175, 503
SmoI CTYRAG 3 cut(s) 167, 175, 503
Sse9I AATT 9 cut(s) 15, 42, 144, 228, 343, 354, 361, 556, 585
SsiI CCGC 3 cut(s) 152, 222, 420
TaaI ACNGT 2 cut(s) 721, 807
TaqI TCGA 6 cut(s) 105, 193, 243, 592, 728, 824
TasI AATT 9 cut(s) 15, 42, 144, 228, 343, 354, 361, 556, 585
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 491, 504
Tru9I TTAA 2 cut(s) 491, 504
TseI GCWGC 5 cut(s) 311, 385, 422, 704, 796
TspDTI ATGAA 3 cut(s) 665, 682, 851
Vha464I CTTAAG 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 476, 780
XapI RAATTY 2 cut(s) 15, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.