Rh4BG358400

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009555
Physical Location & Seq
Forward (+)
51833029 .. 51835310
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG358400.1

Sequence Viewer

Length: 885 bp
ATGATTCCTCTGCAAATTCACCACCTCTCTCCACCCTTGTCAATTTCCCAAACTACCCCTCTCTCCTCTCTCCCTTCCCTTCCTCACCTCCTCTCTCAAAGACTCGACCTTTCCATCAAACCCAACCCCTTCACCTTCTCCCCAATTTCACTGCCCAAACCCACCTTCAAGCTCTCAAGGGCATCGCAAAGTCGATCGCTTTTCTTCGGAAATGAGGAGGACACCGCCGGAATTTCAACTGACCTCGACGACTTGTCCCCAAACGGCGTCGTTCACCGGAAAACATTGGCATTGGTTGAGTGCTCCATGTTTGCTGCGCTTACTGGGTTGGTGTATTTCCTCAGCAATTCTCTCGCAATTGAGAATTACTTTGGTTGCTTCTTTTCGCTGCCGATAGTGATCACCTCAATGAGATGGGGCACCGCAGCTGGAAGAAAAACTATGGTGGCAACTACTATGCTATTACTTGTCTTGTCTGGTCCGGTGAAAGCCTTAACCTATTTGCTTAAGCATGGATTAGTTGGCTTTACAATGGGAAGTTTGTGGAGGTCGGGAGCAAATTGGACTATTTCAGTTTTCTTGTGCGCAATTGTTCGAGCATTGGGTTCTGTAGGGTATGTTTTGATCACTTCTTTCTTGATCAGAGAAAACATATTTGCTCTGATCACCATAAACATTCATGCTTCTCTAACATTCATCTTCACAGCAGCAGGCATCTATACAGTTCCATCGATGGAAGCAATATATGCCTTATTTAGTATTCTGGTTTTTATAAATAGTGGGTCCTTCACGTTCTTGCTGCACATACTGTATGGTGCCTTCCTTTCGAGGCTCGGGATGAAAGCATCTTTGAGATTGCCAAGATGGCTGGAGAAGGCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

294

Amino Acids

32.21

Weight (kDa)

9.71

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 126 - 281 1.8e-07 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
Acc16I TGCGCA 1 cut(s) 586
AccB1I GGYRCC 2 cut(s) 419, 815
AciI CCGC 2 cut(s) 225, 423
AcsI RAATTY 2 cut(s) 15, 231
AcyI GRCGYC 1 cut(s) 267
AflII CTTAAG 1 cut(s) 506
AgsI TTSAA 2 cut(s) 169, 237
AhdI GACNNNNNGTC 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 2 cut(s) 172, 428
AluI AGCT 2 cut(s) 172, 428
Alw21I GWGCWC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 833
ApeKI GCWGC 5 cut(s) 314, 388, 425, 707, 799
ApoI RAATTY 2 cut(s) 15, 231
AspLEI GCGC 2 cut(s) 319, 587
AspS9I GGNCC 2 cut(s) 479, 783
AsuHPI GGTGA 7 cut(s) 11, 77, 124, 266, 394, 496, 658
AvaI CYCGRG 1 cut(s) 833
AvaII GGWCC 2 cut(s) 479, 783
BaeGI GKGCMC 1 cut(s) 422
BanI GGYRCC 2 cut(s) 419, 815
BarI GAAGNNNNNNTAC 2 cut(s) 803, 835
Bbv12I GWGCWC 1 cut(s) 305
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 5 cut(s) 301, 375, 437, 719, 786
BccI CCATC 5 cut(s) 122, 408, 727, 736, 858
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BclI TGATCA 4 cut(s) 399, 624, 639, 663
BfmI CTRYAG 1 cut(s) 609
BfrI CTTAAG 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 865
BisI GCNGC 5 cut(s) 315, 389, 426, 708, 800
BlsI GCNGC 5 cut(s) 316, 390, 427, 709, 801
Bme18I GGWCC 2 cut(s) 479, 783
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 833
BmgT120I GGNCC 2 cut(s) 479, 783
BmiI GGNNCC 3 cut(s) 421, 784, 817
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 3 cut(s) 191, 723, 854
BmuI ACTGGG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 160
Bsa29I ATCGAT 1 cut(s) 731
BsaBI GATNNNNATC 1 cut(s) 398
BsaHI GRCGYC 1 cut(s) 267
BsaWI WCCGGW 2 cut(s) 276, 481
Bse1I ACTGG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 731
BseGI GGATG 1 cut(s) 843
BseJI GATNNNNATC 1 cut(s) 398
BseMII CTCAG 1 cut(s) 355
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 3 cut(s) 55, 80, 230
BseSI GKGCMC 1 cut(s) 422
BseXI GCAGC 5 cut(s) 301, 375, 437, 719, 786
BsgI GTGCAG 1 cut(s) 785
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 2 cut(s) 419, 815
BshVI ATCGAT 1 cut(s) 731
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 833
BsiSI CCGG 3 cut(s) 228, 277, 482
BslFI GGGAC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 241
BsoBI CYCGRG 1 cut(s) 833
Bsp1286I GDGCHC 2 cut(s) 305, 422
Bsp143I GATC 5 cut(s) 194, 399, 624, 639, 663
BspACI CCGC 2 cut(s) 225, 423
BspCNI CTCAG 1 cut(s) 354
BspDI ATCGAT 1 cut(s) 731
BspLI GGNNCC 3 cut(s) 421, 784, 817
BspT107I GGYRCC 2 cut(s) 419, 815
BspTI CTTAAG 1 cut(s) 506
BsrI ACTGG 1 cut(s) 328
BssMI GATC 5 cut(s) 194, 399, 624, 639, 663
BssNI GRCGYC 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 724, 810
BstACI GRCGYC 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 1 cut(s) 746
BstC8I GCNNGC 1 cut(s) 712
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 843
BstHHI GCGC 2 cut(s) 319, 587
BstKTI GATC 5 cut(s) 197, 402, 627, 642, 666
BstMBI GATC 5 cut(s) 194, 399, 624, 639, 663
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 2 cut(s) 746, 865
BstSFI CTRYAG 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 422
BstV1I GCAGC 5 cut(s) 301, 375, 437, 719, 786
Bsu15I ATCGAT 1 cut(s) 731
BsuTUI ATCGAT 1 cut(s) 731
BtgZI GCGATG 1 cut(s) 168
BtsCI GGATG 1 cut(s) 843
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 712
CfoI GCGC 2 cut(s) 319, 587
Cfr13I GGNCC 2 cut(s) 479, 783
ClaI ATCGAT 1 cut(s) 731
CseI GACGC 1 cut(s) 256
CviAII CATG 3 cut(s) 307, 512, 680
CviJI RGCY 6 cut(s) 172, 428, 491, 525, 832, 868
CviKI_1 RGCY 6 cut(s) 172, 428, 491, 525, 832, 868
DdeI CTNAG 1 cut(s) 341
DpnI GATC 5 cut(s) 196, 401, 626, 641, 665
DpnII GATC 5 cut(s) 194, 399, 624, 639, 663
DriI GACNNNNNGTC 1 cut(s) 253
Eam1105I GACNNNNNGTC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 479, 783
Eco88I CYCGRG 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 783
FaeI CATG 3 cut(s) 310, 515, 683
FaqI GGGAC 1 cut(s) 241
FatI CATG 3 cut(s) 306, 511, 679
FbaI TGATCA 4 cut(s) 399, 624, 639, 663
Fnu4HI GCNGC 5 cut(s) 315, 389, 426, 708, 800
FokI GGATG 1 cut(s) 850
Fsp4HI GCNGC 5 cut(s) 315, 389, 426, 708, 800
FspI TGCGCA 1 cut(s) 586
GlaI GCGC 2 cut(s) 318, 586
GluI GCNGC 5 cut(s) 315, 389, 426, 708, 800
HapII CCGG 3 cut(s) 228, 277, 482
HgaI GACGC 1 cut(s) 256
HhaI GCGC 2 cut(s) 319, 587
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 310, 515, 683
Hin6I GCGC 2 cut(s) 317, 585
HinP1I GCGC 2 cut(s) 317, 585
HinfI GANTC 2 cut(s) 4, 102
HpaII CCGG 3 cut(s) 228, 277, 482
HphI GGTGA 7 cut(s) 11, 77, 124, 266, 394, 496, 658
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 3 cut(s) 209, 644, 663
Hpy188III TCNNGA 3 cut(s) 552, 637, 835
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 251, 272
HpyAV CCTTC 8 cut(s) 84, 89, 139, 145, 175, 796, 829, 868
HpyCH4III ACNGT 2 cut(s) 724, 810
HpyCH4IV ACGT 1 cut(s) 791
HpyCH4V TGCA 2 cut(s) 13, 802
HpyF10VI GCNNNNNNNGC 2 cut(s) 746, 865
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 1 cut(s) 791
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 310, 515, 683
HspAI GCGC 2 cut(s) 317, 585
Ksp22I TGATCA 4 cut(s) 399, 624, 639, 663
Kzo9I GATC 5 cut(s) 194, 399, 624, 639, 663
LmnI GCTCC 2 cut(s) 308, 554
LpnPI CCDG 9 cut(s) 241, 290, 309, 414, 462, 495, 696, 749, 854
Lsp1109I GCAGC 5 cut(s) 301, 375, 437, 719, 786
LweI GCATC 3 cut(s) 191, 723, 854
MaeII ACGT 1 cut(s) 791
MalI GATC 5 cut(s) 196, 401, 626, 641, 665
MboI GATC 5 cut(s) 194, 399, 624, 639, 663
MboII GAAGA 3 cut(s) 196, 444, 691
MfeI CAATTG 2 cut(s) 357, 588
MhlI GDGCHC 2 cut(s) 305, 422
MluCI AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
MlyI GAGTC 1 cut(s) 96
MseI TTAA 2 cut(s) 494, 507
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 428
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 3 cut(s) 228, 277, 482
MunI CAATTG 2 cut(s) 357, 588
MwoI GCNNNNNNNGC 2 cut(s) 746, 865
NdeII GATC 5 cut(s) 194, 399, 624, 639, 663
NlaIII CATG 3 cut(s) 310, 515, 683
NlaIV GGNNCC 3 cut(s) 421, 784, 817
NsbI TGCGCA 1 cut(s) 586
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 5 cut(s) 316, 390, 427, 709, 801
Ple19I CGATCG 1 cut(s) 197
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 783
PsiI TTATAA 1 cut(s) 773
Psp5II RGGWCCY 1 cut(s) 783
PspN4I GGNNCC 3 cut(s) 421, 784, 817
PspPI GGNCC 2 cut(s) 479, 783
PspPPI RGGWCCY 1 cut(s) 783
PvuI CGATCG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 2 cut(s) 494, 507
SatI GCNGC 5 cut(s) 315, 389, 426, 708, 800
Sau3AI GATC 5 cut(s) 194, 399, 624, 639, 663
Sau96I GGNCC 2 cut(s) 479, 783
SchI GAGTC 1 cut(s) 96
SduI GDGCHC 2 cut(s) 305, 422
SfaNI GCATC 3 cut(s) 191, 723, 854
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 2 cut(s) 479, 783
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 175, 506
SmoI CTYRAG 2 cut(s) 175, 506
Sse9I AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
SsiI CCGC 2 cut(s) 225, 423
TaaI ACNGT 2 cut(s) 724, 810
TaiI ACGT 1 cut(s) 794
TaqI TCGA 6 cut(s) 105, 193, 246, 595, 731, 827
TasI AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 494, 507
Tru9I TTAA 2 cut(s) 494, 507
TscAI CASTG 1 cut(s) 156
TseI GCWGC 5 cut(s) 314, 388, 425, 707, 799
TspDTI ATGAA 3 cut(s) 668, 685, 854
TspRI CASTG 1 cut(s) 156
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 479, 783
XapI RAATTY 2 cut(s) 15, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.