Rh4CG372800

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009555
Physical Location & Seq
Forward (+)
62734561 .. 62736863
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG372800.1

Sequence Viewer

Length: 753 bp
ATGATTCCTCTGCAAATTCACCACCTCTCTCCACCCTTGTCAATTTCCCAAACTACCCCTCTCTCCTCTCTCCCTTCCCTTCCTCACCTCCTCTCTCAAAGACTCGACCTTTCCATCAAACCCAACCCCTTCACCTTCTCCCCAATTTCACTGCCCAAACCCACCTTCAAGCTCTCAAGGGCATCGCAAAGTCGATCGCTTTTCTTCGGAAATGAGGAGGACACCGCCGGAATTTCAACTGACCTCGACGACTTGTCCCCAAACGGCGTCGTTCACCGGAAAACATTGGCATTGGTTGAGTGCTCCATGTTTGCTGCGCTTACTGGGTTGGTGTATTTCCTCAGCAATTCTCTCGCAATTGAGAATTACTTTGGTTGCTTCTTTTCGCTGCCGATAGTGATCACCTCAATGAGATGGGGCACCGCAGCTGGAAGAAAAACTATGGTGGCAACTACTATGCTATTACTTGTCTTGTCTGGTCCGGTGAAAGCCTTAACCTATTTGCTTAAGCATGGATTAGTTGGCTTTACAATGGGAAGTTTGTGGAGGTCGGGAGCAAATTGGACTATTTCAGTTTTCTTGTGCGCAATTGTTCGAGCATTGGGTTCTGTAGGGTATGTTTTGATCACTTCTTTCTTGATCAGAGAAAACATATTTGCTCTGGTAATGCTTTCTCCTAATATTTGCTTGATGCAGTATCAAGATTCAAGTTACATTTCTAATGGTACACATTTTGCTTGCAGATCACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

250

Amino Acids

27.32

Weight (kDa)

9.22

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 125 - 223 3.9e-08 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 586
AccB1I GGYRCC 1 cut(s) 419
AciI CCGC 2 cut(s) 225, 423
AcsI RAATTY 2 cut(s) 15, 231
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 1 cut(s) 727
AflII CTTAAG 1 cut(s) 506
AgsI TTSAA 3 cut(s) 169, 237, 708
AhdI GACNNNNNGTC 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 2 cut(s) 172, 428
AluI AGCT 2 cut(s) 172, 428
Alw21I GWGCWC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 314, 388, 425
ApoI RAATTY 2 cut(s) 15, 231
AspLEI GCGC 2 cut(s) 319, 587
AspS9I GGNCC 1 cut(s) 479
AsuHPI GGTGA 7 cut(s) 11, 77, 124, 266, 394, 496, 738
AvaII GGWCC 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 422
BanI GGYRCC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 305
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 3 cut(s) 301, 375, 437
BccI CCATC 2 cut(s) 122, 408
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BclI TGATCA 3 cut(s) 399, 624, 639
BfmI CTRYAG 1 cut(s) 609
BfrI CTTAAG 1 cut(s) 506
BisI GCNGC 3 cut(s) 315, 389, 426
BlsI GCNGC 3 cut(s) 316, 390, 427
Bme18I GGWCC 1 cut(s) 479
BmeRI GACNNNNNGTC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 421
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 2 cut(s) 191, 681
BmuI ACTGGG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 398
BsaHI GRCGYC 1 cut(s) 267
BsaWI WCCGGW 2 cut(s) 276, 481
Bse1I ACTGG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 398
BseJI GATNNNNATC 1 cut(s) 398
BseMII CTCAG 1 cut(s) 355
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 3 cut(s) 55, 80, 230
BseSI GKGCMC 1 cut(s) 422
BseXI GCAGC 3 cut(s) 301, 375, 437
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 419
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 305
BsiSI CCGG 3 cut(s) 228, 277, 482
BslFI GGGAC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 241
Bsp1286I GDGCHC 2 cut(s) 305, 422
Bsp143I GATC 5 cut(s) 194, 399, 624, 639, 743
BspACI CCGC 2 cut(s) 225, 423
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 419
BspTI CTTAAG 1 cut(s) 506
BsrI ACTGG 1 cut(s) 328
BssMI GATC 5 cut(s) 194, 399, 624, 639, 743
BssNI GRCGYC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 267
BstAFI CTTAAG 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 739
BstDEI CTNAG 1 cut(s) 341
BstHHI GCGC 2 cut(s) 319, 587
BstKTI GATC 5 cut(s) 197, 402, 627, 642, 746
BstMBI GATC 5 cut(s) 194, 399, 624, 639, 743
BstMCI CGRYCG 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 422
BstV1I GCAGC 3 cut(s) 301, 375, 437
BtgZI GCGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 739
CfoI GCGC 2 cut(s) 319, 587
Cfr13I GGNCC 1 cut(s) 479
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 726
CviAII CATG 2 cut(s) 307, 512
CviJI RGCY 4 cut(s) 172, 428, 491, 525
CviKI_1 RGCY 4 cut(s) 172, 428, 491, 525
CviQI GTAC 1 cut(s) 726
DdeI CTNAG 1 cut(s) 341
DpnI GATC 5 cut(s) 196, 401, 626, 641, 745
DpnII GATC 5 cut(s) 194, 399, 624, 639, 743
DriI GACNNNNNGTC 1 cut(s) 253
Eam1105I GACNNNNNGTC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 479
FaeI CATG 2 cut(s) 310, 515
FaiI YATR 7 cut(s) 308, 443, 458, 513, 618, 653, 751
FaqI GGGAC 1 cut(s) 241
FatI CATG 2 cut(s) 306, 511
FbaI TGATCA 3 cut(s) 399, 624, 639
Fnu4HI GCNGC 3 cut(s) 315, 389, 426
Fsp4HI GCNGC 3 cut(s) 315, 389, 426
FspI TGCGCA 1 cut(s) 586
GlaI GCGC 2 cut(s) 318, 586
GluI GCNGC 3 cut(s) 315, 389, 426
HapII CCGG 3 cut(s) 228, 277, 482
HgaI GACGC 1 cut(s) 256
HhaI GCGC 2 cut(s) 319, 587
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 2 cut(s) 310, 515
Hin6I GCGC 2 cut(s) 317, 585
HinP1I GCGC 2 cut(s) 317, 585
HinfI GANTC 3 cut(s) 4, 102, 704
HpaII CCGG 3 cut(s) 228, 277, 482
HphI GGTGA 7 cut(s) 11, 77, 124, 266, 394, 496, 738
Hpy166II GTNNAC 2 cut(s) 274, 728
Hpy188I TCNGA 2 cut(s) 209, 644
Hpy188III TCNNGA 3 cut(s) 552, 637, 701
Hpy8I GTNNAC 2 cut(s) 274, 728
Hpy99I CGWCG 2 cut(s) 251, 272
HpyAV CCTTC 5 cut(s) 84, 89, 139, 145, 175
HpyCH4V TGCA 3 cut(s) 13, 694, 741
HpyF3I CTNAG 1 cut(s) 341
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 2 cut(s) 310, 515
HspAI GCGC 2 cut(s) 317, 585
Ksp22I TGATCA 3 cut(s) 399, 624, 639
Kzo9I GATC 5 cut(s) 194, 399, 624, 639, 743
LmnI GCTCC 2 cut(s) 308, 554
LpnPI CCDG 7 cut(s) 241, 290, 309, 414, 462, 495, 647
Lsp1109I GCAGC 3 cut(s) 301, 375, 437
LweI GCATC 2 cut(s) 191, 681
MaeIII GTNAC 1 cut(s) 710
MalI GATC 5 cut(s) 196, 401, 626, 641, 745
MboI GATC 5 cut(s) 194, 399, 624, 639, 743
MboII GAAGA 2 cut(s) 196, 444
MfeI CAATTG 2 cut(s) 357, 588
MhlI GDGCHC 2 cut(s) 305, 422
MluCI AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
MlyI GAGTC 1 cut(s) 96
MseI TTAA 2 cut(s) 494, 507
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 428
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 3 cut(s) 228, 277, 482
MunI CAATTG 2 cut(s) 357, 588
NdeII GATC 5 cut(s) 194, 399, 624, 639, 743
NlaIII CATG 2 cut(s) 310, 515
NlaIV GGNNCC 1 cut(s) 421
NsbI TGCGCA 1 cut(s) 586
PfeI GAWTC 2 cut(s) 4, 704
PkrI GCNGC 3 cut(s) 316, 390, 427
Ple19I CGATCG 1 cut(s) 197
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 479
PvuI CGATCG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 2 cut(s) 494, 507
SatI GCNGC 3 cut(s) 315, 389, 426
Sau3AI GATC 5 cut(s) 194, 399, 624, 639, 743
Sau96I GGNCC 1 cut(s) 479
SchI GAGTC 1 cut(s) 96
SduI GDGCHC 2 cut(s) 305, 422
SfaNI GCATC 2 cut(s) 191, 681
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 175, 506
SmoI CTYRAG 2 cut(s) 175, 506
Sse9I AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
SsiI CCGC 2 cut(s) 225, 423
SspI AATATT 1 cut(s) 682
TaqI TCGA 4 cut(s) 105, 193, 246, 595
TasI AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
TfiI GAWTC 2 cut(s) 4, 704
Tru1I TTAA 2 cut(s) 494, 507
Tru9I TTAA 2 cut(s) 494, 507
TscAI CASTG 1 cut(s) 156
TseI GCWGC 3 cut(s) 314, 388, 425
TspRI CASTG 1 cut(s) 156
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 479
XapI RAATTY 2 cut(s) 15, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.