MD13G1052300.v1.1

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009555
Physical Location & Seq
Forward (+)
3703736 .. 3706199
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1052300.v1.1.491

Sequence Viewer

Length: 879 bp
ATGATTCCTCTGCAAATCCACCACCTCACCCCACGTTTCCTCATTTCCCAAACTACCCCTCTCCACTCCGCTCTCTCCCCCACCTCTCAAGCTGTCAAATTTTCCCTCAAACCCTACCCCTTTTCGTTTTCCCCAATTTTGCTATCCAACCCCACCTGCAAATTTCTGAAAGCCGCAGGGAGCCAAACGTCGTCGCTTTCTAGAAATGAGGAAGAAAGCGCTGGAATCTCAACCGGGCTCGAAGACTTGTCGGCGGACGGCGTCGTTTATAAGAAGACATTAGCGTTGGTGGAGTGCTCCATGTTTGCTGCGCTCACTGGCTTGGTGTACTTCCTCAGCAATTCTCTCGCAATTGAGAATTATTTTGGCTGCTTCTTCTCGCTGCCGATAGTGATCACCTCAATGAGATGGGGCATCGCCACTGGAAGAAAAACTATGGTTGCAACAACTATGCTGTTGTTAATCCTGTCTGGTCCGGTGAAAGCCTTAACCTATCTGCTTAAGCATGGTTTAGTTGGTTTCACAATGGGAAGTTTATGGAGGTCGGGAGCAAACTGGAGTCTTTCTATTTTCTTGTGCACAATTGTTCGATCACTGGGTCTTGTAGGGTATGTCCTGATATCGTCGTTCTTGATAAGTGAAAACATACTTGCTTTGATCACTATAAACATTCATGCTTCTCTAACATACATATTCACTGCATCGGGCATCTATACAGTCCCGTCAATGAAAGCAATTTATGTCTTATTTGGTACTCTGGTATTGATTAATTGCGGATCCTTCGTGTTCTTGCTACACCTATTATATTCGGTGTTCCTTATGAGGCTTGGGATGAAAGCATCATTAAGATTGCCAAGATGGCTGGAGAGGGCTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

293

Amino Acids

32.0

Weight (kDa)

9.6

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 124 - 279 1.1e-07 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 4 cut(s) 69, 174, 254, 774
AclWI GGATC 2 cut(s) 771, 784
AcsI RAATTY 2 cut(s) 98, 161
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 2 cut(s) 329, 754
AfeI AGCGCT 1 cut(s) 220
AflII CTTAAG 1 cut(s) 500
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
Alw21I GWGCWC 2 cut(s) 299, 581
Alw44I GTGCAC 1 cut(s) 577
AlwI GGATC 2 cut(s) 771, 784
Aor51HI AGCGCT 1 cut(s) 220
ApaLI GTGCAC 1 cut(s) 577
ApeKI GCWGC 3 cut(s) 308, 369, 382
ApoI RAATTY 2 cut(s) 98, 161
AseI ATTAAT 1 cut(s) 768
AspLEI GCGC 2 cut(s) 221, 313
AspS9I GGNCC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 3 cut(s) 19, 388, 490
AvaII GGWCC 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 581
BaeI ACNNNNGTAYC 2 cut(s) 744, 777
BamHI GGATCC 1 cut(s) 776
BanII GRGCYC 1 cut(s) 240
BbsI GAAGAC 2 cut(s) 249, 281
Bbv12I GWGCWC 2 cut(s) 299, 581
BbvCI CCTCAGC 1 cut(s) 335
BbvI GCAGC 3 cut(s) 295, 356, 369
BccI CCATC 2 cut(s) 402, 852
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BclI TGATCA 2 cut(s) 393, 657
BcnI CCSGG 1 cut(s) 235
BfaI CTAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 500
BfuAI ACCTGC 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 859
BisI GCNGC 4 cut(s) 174, 309, 370, 383
BlsI GCNGC 4 cut(s) 175, 310, 371, 384
Bme1390I CCNGG 1 cut(s) 235
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 2 cut(s) 182, 778
BmrFI CCNGG 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 605
BmsI GCATC 4 cut(s) 423, 710, 717, 848
BmuI ACTGGG 1 cut(s) 605
BpiI GAAGAC 2 cut(s) 249, 281
BpmI CTGGAG 1 cut(s) 577
Bpu10I CCTNAGC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 235
BsaBI GATNNNNATC 1 cut(s) 392
BsaHI GRCGYC 1 cut(s) 261
BsaWI WCCGGW 1 cut(s) 475
Bse1I ACTGG 4 cut(s) 322, 427, 560, 600
Bse8I GATNNNNATC 1 cut(s) 392
BseGI GGATG 1 cut(s) 837
BseJI GATNNNNATC 1 cut(s) 392
BseMII CTCAG 1 cut(s) 349
BseNI ACTGG 4 cut(s) 322, 427, 560, 600
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 3 cut(s) 295, 356, 369
BsiHKAI GWGCWC 2 cut(s) 299, 581
BsiSI CCGG 2 cut(s) 234, 476
BslFI GGGAC 1 cut(s) 704
BsmFI GGGAC 1 cut(s) 704
Bsp1286I GDGCHC 3 cut(s) 240, 299, 581
Bsp143I GATC 4 cut(s) 393, 590, 657, 776
BspACI CCGC 4 cut(s) 69, 174, 254, 774
BspCNI CTCAG 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 182, 778
BspMI ACCTGC 1 cut(s) 164
BspPI GGATC 2 cut(s) 771, 784
BspTI CTTAAG 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 71
BsrI ACTGG 4 cut(s) 322, 427, 560, 600
BssMI GATC 4 cut(s) 393, 590, 657, 776
BssNI GRCGYC 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 718
BstACI GRCGYC 1 cut(s) 261
BstAFI CTTAAG 1 cut(s) 500
BstDEI CTNAG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 837
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 313
BstKTI GATC 4 cut(s) 396, 593, 660, 779
BstMBI GATC 4 cut(s) 393, 590, 657, 776
BstMWI GCNNNNNNNGC 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 581
BstV1I GCAGC 3 cut(s) 295, 356, 369
BstV2I GAAGAC 2 cut(s) 249, 281
BstX2I RGATCY 1 cut(s) 776
BstYI RGATCY 1 cut(s) 776
BtgZI GCGATG 1 cut(s) 400
BtsCI GGATG 1 cut(s) 837
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 4 cut(s) 315, 420, 593, 696
BveI ACCTGC 1 cut(s) 164
CfoI GCGC 2 cut(s) 221, 313
Cfr13I GGNCC 1 cut(s) 473
CseI GACGC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 328, 753
CviAII CATG 3 cut(s) 301, 506, 674
CviQI GTAC 2 cut(s) 328, 753
DdeI CTNAG 1 cut(s) 335
DpnI GATC 4 cut(s) 395, 592, 659, 778
DpnII GATC 4 cut(s) 393, 590, 657, 776
EciI GGCGGA 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 240
Eco32I GATATC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 473
Eco47III AGCGCT 1 cut(s) 220
EcoRV GATATC 1 cut(s) 621
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 3 cut(s) 304, 509, 677
FaqI GGGAC 1 cut(s) 704
FatI CATG 3 cut(s) 300, 505, 673
FbaI TGATCA 2 cut(s) 393, 657
Fnu4HI GCNGC 4 cut(s) 174, 309, 370, 383
FokI GGATG 1 cut(s) 844
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 4 cut(s) 174, 309, 370, 383
FspBI CTAG 1 cut(s) 201
GlaI GCGC 2 cut(s) 220, 312
GluI GCNGC 4 cut(s) 174, 309, 370, 383
GsuI CTGGAG 1 cut(s) 577
HaeII RGCGCY 1 cut(s) 222
HapII CCGG 2 cut(s) 234, 476
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 221, 313
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 3 cut(s) 304, 509, 677
Hin6I GCGC 2 cut(s) 219, 311
HinP1I GCGC 2 cut(s) 219, 311
HinfI GANTC 3 cut(s) 4, 225, 559
HpaII CCGG 2 cut(s) 234, 476
HphI GGTGA 3 cut(s) 19, 388, 490
Hpy166II GTNNAC 2 cut(s) 328, 579
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 4 cut(s) 201, 546, 616, 631
Hpy8I GTNNAC 2 cut(s) 328, 579
Hpy99I CGWCG 4 cut(s) 193, 196, 266, 628
HpyAV CCTTC 1 cut(s) 790
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 2 cut(s) 34, 188
HpyCH4V TGCA 5 cut(s) 13, 159, 443, 579, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 859
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 2 cut(s) 34, 188
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 3 cut(s) 304, 509, 677
HspAI GCGC 2 cut(s) 219, 311
Ksp22I TGATCA 2 cut(s) 393, 657
Kzo9I GATC 4 cut(s) 393, 590, 657, 776
LmnI GCTCC 3 cut(s) 180, 302, 548
Lsp1109I GCAGC 3 cut(s) 295, 356, 369
LweI GCATC 4 cut(s) 423, 710, 717, 848
MaeI CTAG 1 cut(s) 201
MaeII ACGT 2 cut(s) 34, 188
MalI GATC 4 cut(s) 395, 592, 659, 778
MbiI CCGCTC 1 cut(s) 71
MboI GATC 4 cut(s) 393, 590, 657, 776
MboII GAAGA 5 cut(s) 224, 254, 286, 367, 438
MfeI CAATTG 2 cut(s) 351, 582
MflI RGATCY 1 cut(s) 776
MhlI GDGCHC 3 cut(s) 240, 299, 581
MluCI AATT 9 cut(s) 98, 135, 161, 340, 351, 358, 582, 735, 769
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 1 cut(s) 171
MseI TTAA 5 cut(s) 461, 488, 501, 768, 845
MslI CAYNNNNRTG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 2 cut(s) 234, 476
MspR9I CCNGG 1 cut(s) 235
MunI CAATTG 2 cut(s) 351, 582
MwoI GCNNNNNNNGC 1 cut(s) 859
NciI CCSGG 1 cut(s) 235
NdeII GATC 4 cut(s) 393, 590, 657, 776
NlaIII CATG 3 cut(s) 304, 509, 677
NlaIV GGNNCC 2 cut(s) 182, 778
PaqCI CACCTGC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 4, 225
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 4 cut(s) 175, 310, 371, 384
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
PshBI ATTAAT 1 cut(s) 768
PsiI TTATAA 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 182, 778
PspPI GGNCC 1 cut(s) 473
PsuI RGATCY 1 cut(s) 776
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 2 cut(s) 329, 754
RsaNI GTAC 2 cut(s) 328, 753
RseI CAYNNNNRTG 1 cut(s) 401
SaqAI TTAA 5 cut(s) 461, 488, 501, 768, 845
SatI GCNGC 4 cut(s) 174, 309, 370, 383
Sau3AI GATC 4 cut(s) 393, 590, 657, 776
Sau96I GGNCC 1 cut(s) 473
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 3 cut(s) 240, 299, 581
SfaNI GCATC 4 cut(s) 423, 710, 717, 848
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 87, 500
SmoI CTYRAG 2 cut(s) 87, 500
Sse9I AATT 9 cut(s) 98, 135, 161, 340, 351, 358, 582, 735, 769
SsiI CCGC 4 cut(s) 69, 174, 254, 774
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 233
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 2 cut(s) 37, 191
TaqI TCGA 2 cut(s) 240, 589
TasI AATT 9 cut(s) 98, 135, 161, 340, 351, 358, 582, 735, 769
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 2 cut(s) 4, 225
Tru1I TTAA 5 cut(s) 461, 488, 501, 768, 845
Tru9I TTAA 5 cut(s) 461, 488, 501, 768, 845
TscAI CASTG 4 cut(s) 322, 427, 600, 703
TseI GCWGC 3 cut(s) 308, 369, 382
TspDTI ATGAA 3 cut(s) 662, 743, 848
TspRI CASTG 4 cut(s) 322, 427, 600, 703
Tth111I GACNNNGTC 1 cut(s) 260
Vha464I CTTAAG 1 cut(s) 500
VneI GTGCAC 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 473
VspI ATTAAT 1 cut(s) 768
XapI RAATTY 2 cut(s) 98, 161
XbaI TCTAGA 1 cut(s) 200
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.