Rh4AG040800

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009555
Physical Location & Seq
Reverse (-)
8546353 .. 8551169
4817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG040800.1

Sequence Viewer

Length: 336 bp
ATGTTTGCTGCGCTTACTGGGTTGGTGTATTTCCTCAGCAATTCTCTCGCAATTGAGAATTACTTTGGTTGCTTCTTTTCGCTGCCGATAGTGATCACCTCAATGAGATGGGGCACTGCAGCTGGAAGAAAAACTATGGTGGCAACGACTATACTATTACTTGTCTTGTCTGGTCCGGTGAAAGCCTTAACCTATTTGCTTAAGCATGGATTAGTTGGCTTTACAATGGGAAGTTTGTGGAGGTCTGGAACAAATTGGACTATTTCAGTTTTCTTGTGCGCAATTGTTCGAGCATTGGGTTCTGTAGGGACGTGTCTGTTACTTGACAATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

111

Amino Acids

11.96

Weight (kDa)

9.57

Isoelectric Point (pI)

18.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 23 - 108 1.8e-07 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 280
AflII CTTAAG 1 cut(s) 200
AflIII ACRYGT 1 cut(s) 311
AjiI CACGTC 1 cut(s) 312
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
ApeKI GCWGC 3 cut(s) 8, 82, 119
AspLEI GCGC 2 cut(s) 13, 281
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 88, 190
AvaII GGWCC 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 116
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 69, 131
BccI CCATC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BclI TGATCA 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 117, 303
BfrI CTTAAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 9, 83, 120
BlsI GCNGC 3 cut(s) 10, 84, 121
Bme18I GGWCC 1 cut(s) 173
BmgBI CACGTC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 27
BmuI ACTGGG 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 92
BsaWI WCCGGW 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 22
Bse8I GATNNNNATC 1 cut(s) 92
BseJI GATNNNNATC 1 cut(s) 92
BseMII CTCAG 1 cut(s) 49
BseNI ACTGG 1 cut(s) 22
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 2 cut(s) 69, 131
BsiSI CCGG 1 cut(s) 176
BslFI GGGAC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 48
BspMAI CTGCAG 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 200
BsrI ACTGG 1 cut(s) 22
BssMI GATC 1 cut(s) 93
BstAFI CTTAAG 1 cut(s) 200
BstDEI CTNAG 1 cut(s) 35
BstHHI GCGC 2 cut(s) 13, 281
BstKTI GATC 1 cut(s) 96
BstMBI GATC 1 cut(s) 93
BstSFI CTRYAG 2 cut(s) 117, 303
BstSLI GKGCMC 1 cut(s) 116
BstV1I GCAGC 2 cut(s) 69, 131
BtrI CACGTC 1 cut(s) 312
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 114
CfoI GCGC 2 cut(s) 13, 281
Cfr13I GGNCC 1 cut(s) 173
CviAII CATG 1 cut(s) 206
CviJI RGCY 3 cut(s) 122, 185, 219
CviKI_1 RGCY 3 cut(s) 122, 185, 219
DdeI CTNAG 1 cut(s) 35
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 173
FaeI CATG 1 cut(s) 209
FaiI YATR 3 cut(s) 137, 152, 207
FaqI GGGAC 1 cut(s) 322
FatI CATG 1 cut(s) 205
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 9, 83, 120
Fsp4HI GCNGC 3 cut(s) 9, 83, 120
FspI TGCGCA 1 cut(s) 280
GlaI GCGC 2 cut(s) 12, 280
GluI GCNGC 3 cut(s) 9, 83, 120
HapII CCGG 1 cut(s) 176
HhaI GCGC 2 cut(s) 13, 281
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 2 cut(s) 11, 279
HinP1I GCGC 2 cut(s) 11, 279
HpaII CCGG 1 cut(s) 176
HphI GGTGA 2 cut(s) 88, 190
Hpy188III TCNNGA 1 cut(s) 246
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 1 cut(s) 119
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 2 cut(s) 11, 279
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 1 cut(s) 93
LpnPI CCDG 5 cut(s) 3, 108, 156, 189, 231
Lsp1109I GCAGC 2 cut(s) 69, 131
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 318
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MboII GAAGA 1 cut(s) 138
MfeI CAATTG 2 cut(s) 51, 282
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 5 cut(s) 40, 51, 58, 253, 282
MnlI CCTC 3 cut(s) 44, 109, 234
MseI TTAA 2 cut(s) 188, 201
MslI CAYNNNNRTG 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 122
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 1 cut(s) 176
MunI CAATTG 2 cut(s) 51, 282
NdeII GATC 1 cut(s) 93
NlaIII CATG 1 cut(s) 209
NsbI TGCGCA 1 cut(s) 280
PkrI GCNGC 3 cut(s) 10, 84, 121
PspPI GGNCC 1 cut(s) 173
PstI CTGCAG 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 2 cut(s) 188, 201
SatI GCNGC 3 cut(s) 9, 83, 120
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 1 cut(s) 173
SduI GDGCHC 1 cut(s) 116
SetI ASST 5 cut(s) 101, 124, 194, 245, 314
SfcI CTRYAG 2 cut(s) 117, 303
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 5 cut(s) 40, 51, 58, 253, 282
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 289
TasI AATT 5 cut(s) 40, 51, 58, 253, 282
Tru1I TTAA 2 cut(s) 188, 201
Tru9I TTAA 2 cut(s) 188, 201
TscAI CASTG 1 cut(s) 121
TseI GCWGC 3 cut(s) 8, 82, 119
TspRI CASTG 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.