Rorug04G0295200

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009555
Physical Location & Seq
Reverse (-)
46945929 .. 46946969
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0295200.1

Sequence Viewer

Length: 1041 bp
ATGTCTAGGGTTCCATTAAGTGATGCTGAGGCAGCTGAGCAAGAGGAAAGATTTCAGAGGGCACTTGCTGAGTACATTGCTGATCCAGACACTCCACCTAACCTTCTTGAAGGCGTTGGATTTAGAAGATTTTTGGAGGTGCTTAACCCGCGGTTTAAGCCTAATTTCAAAGAGGTTGGGAGTGAATTCTGCAAAATCTATAAGGAAAGGAAAGCGGGAGTTAAGGAATTCTTGGGAAAATTTGATGGAATGATTAGCTTGTCGGTGGAGATATTGAGGCATGAAAATCCTAGTAACGGCTTCTGTCCTGATGATTACTTGTGCCTCTCTGCTCATTTTGTTGATGAGAAGTGGAAACTGCAAAAGTGGGTGCTTCACTCTCGTGGTCTTATGCTTAAGGAGGATTTGGGCCCGTCTGCTGTTCTTTATGACGATGATGATTGGGGTGTTTTTAGGTGGCTTGAGGAGTTTGGCATTGAGAAGAAGATATCCACACTCGCTATGATTAATGATGATGTTGGCTACGATCAATTGGTTGGGTGCGTGAAGAATCACATTCAAGAAAAGAAGGGAATCCAACTTAATGATCAGTTGTTTCGTGTGTATTGTTTTGAGGAAATGATAGCCACAATGGTGCAGGGTGCATTTAACAAGATTAAAAAAATAGTTGACAAGCTATCTTTCTTGTGTGGTTTTGTATCTATAACTCCTGTGTGGAATGTGACAAATTCCCATCTAAAAGAAGCTCTGGAGTTATGGTCAGCGGGAGAATACTCTTCAGTTGACACTGATGTTGTACCCACCCCTGAGGAATGGAAGAAAGTTGAAGGAGTTTGTAAGATCGTAGACAGCATATATGAAGTGTCAAATGCTTTATTTCAAGCAAAGCAACTGACTGCTAATGTTTATCTTTATCACCTACATGAGCTTCATGAAATTCTGACCCAAGCATCGGTTGACTCTGATAGTTTTGTTAATACAGTAGTTAAGGGTATGTTGAAAGCTTTTGACAGGTATTGGGATAAAATGTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

346

Amino Acids

39.76

Weight (kDa)

5.13

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 296 - 345 2.1e-06 hAT-like transposase, RNase-H fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 846
AccII CGCG 1 cut(s) 151
AciI CCGC 4 cut(s) 149, 151, 215, 764
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 5 cut(s) 185, 227, 239, 727, 936
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 2 cut(s) 74, 798
AfiI CCNNNNNNNGG 2 cut(s) 296, 952
AflII CTTAAG 1 cut(s) 395
AgsI TTSAA 6 cut(s) 110, 169, 560, 827, 881, 1000
AleI CACNNNNGTG 2 cut(s) 381, 632
AloI GAACNNNNNNTCC 1 cut(s) 1013
AluBI AGCT 6 cut(s) 35, 258, 676, 746, 928, 1004
AluI AGCT 6 cut(s) 35, 258, 676, 746, 928, 1004
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 409
ApaI GGGCCC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 5 cut(s) 185, 227, 239, 727, 936
AseI ATTAAT 1 cut(s) 507
Asp700I GAANNNNTTC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 409, 410
AsuHPI GGTGA 1 cut(s) 908
AxyI CCTNAGG 1 cut(s) 807
BaeGI GKGCMC 2 cut(s) 64, 413
BanII GRGCYC 1 cut(s) 413
BauI CACGAG 1 cut(s) 381
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 44
BccI CCATC 2 cut(s) 239, 741
BceAI ACGGC 1 cut(s) 313
BclI TGATCA 1 cut(s) 586
BfaI CTAG 2 cut(s) 6, 291
BfrI CTTAAG 1 cut(s) 395
BisI GCNGC 1 cut(s) 33
BlpI GCTNAGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 34
BmgT120I GGNCC 2 cut(s) 409, 410
BmiI GGNNCC 2 cut(s) 12, 411
BmsI GCATC 2 cut(s) 13, 959
BpmI CTGGAG 1 cut(s) 770
Bpu10I CCTNAGC 1 cut(s) 27
Bpu1102I GCTNAGC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 743, 773
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 952
Bse21I CCTNAGG 1 cut(s) 807
Bse3DI GCAATG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 296, 952
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 4 cut(s) 18, 27, 60, 798
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 2 cut(s) 64, 413
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 656
Bsh1236I CGCG 1 cut(s) 151
BshFI GGCC 1 cut(s) 411
BslI CCNNNNNNNGG 2 cut(s) 296, 952
BsnI GGCC 1 cut(s) 411
Bsp120I GGGCCC 1 cut(s) 409
Bsp1286I GDGCHC 2 cut(s) 64, 413
Bsp143I GATC 4 cut(s) 82, 526, 586, 840
Bsp1720I GCTNAGC 1 cut(s) 36
BspACI CCGC 4 cut(s) 149, 151, 215, 764
BspANI GGCC 1 cut(s) 411
BspCNI CTCAG 4 cut(s) 19, 28, 61, 799
BspFNI CGCG 1 cut(s) 151
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 12, 411
BspPI GGATC 1 cut(s) 77
BspTI CTTAAG 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 4 cut(s) 82, 526, 586, 840
BssSI CACGAG 1 cut(s) 381
Bst2BI CACGAG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 982
Bst6I CTCTTC 1 cut(s) 781
BstAFI CTTAAG 1 cut(s) 395
BstDEI CTNAG 4 cut(s) 27, 36, 69, 807
BstDSI CCRYGG 1 cut(s) 149
BstFNI CGCG 1 cut(s) 151
BstKTI GATC 4 cut(s) 85, 529, 589, 843
BstMBI GATC 4 cut(s) 82, 526, 586, 840
BstMWI GCNNNNNNNGC 3 cut(s) 32, 148, 157
BstSLI GKGCMC 2 cut(s) 64, 413
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 44
Bsu36I CCTNAGG 1 cut(s) 807
BsuRI GGCC 1 cut(s) 411
BtgI CCRYGG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 786
CciI TCATGA 1 cut(s) 931
Cfr13I GGNCC 2 cut(s) 409, 410
Cfr42I CCGCGG 1 cut(s) 152
Csp6I GTAC 2 cut(s) 73, 797
CviAII CATG 3 cut(s) 281, 923, 932
CviQI GTAC 2 cut(s) 73, 797
DdeI CTNAG 4 cut(s) 27, 36, 69, 807
DpnI GATC 4 cut(s) 84, 528, 588, 842
DpnII GATC 4 cut(s) 82, 526, 586, 840
Eam1104I CTCTTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 781
Eco24I GRGCYC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 762
Eco81I CCTNAGG 1 cut(s) 807
EcoRI GAATTC 2 cut(s) 185, 227
EcoRV GATATC 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 413
FaeI CATG 3 cut(s) 284, 926, 935
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FatI CATG 3 cut(s) 280, 922, 931
FauI CCCGC 3 cut(s) 156, 208, 757
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 1 cut(s) 33
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 2 cut(s) 6, 291
GluI GCNGC 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 770
HaeIII GGCC 1 cut(s) 411
Hin1II CATG 3 cut(s) 284, 926, 935
HincII GTYRAC 3 cut(s) 670, 784, 958
HindII GTYRAC 3 cut(s) 670, 784, 958
HindIII AAGCTT 1 cut(s) 1002
HinfI GANTC 3 cut(s) 550, 573, 959
HphI GGTGA 1 cut(s) 908
Hpy166II GTNNAC 4 cut(s) 670, 784, 847, 958
Hpy188I TCNGA 3 cut(s) 57, 942, 964
Hpy188III TCNNGA 6 cut(s) 86, 107, 308, 560, 749, 932
Hpy8I GTNNAC 4 cut(s) 670, 784, 847, 958
HpyAV CCTTC 4 cut(s) 104, 113, 562, 821
HpyCH4III ACNGT 1 cut(s) 982
HpyCH4V TGCA 4 cut(s) 192, 361, 637, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 148, 157
HpyF3I CTNAG 4 cut(s) 27, 36, 69, 807
Hsp92II CATG 3 cut(s) 284, 926, 935
Ksp22I TGATCA 1 cut(s) 586
KspI CCGCGG 1 cut(s) 152
Kzo9I GATC 4 cut(s) 82, 526, 586, 840
LpnPI CCDG 7 cut(s) 99, 321, 623, 723, 734, 819, 997
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 2 cut(s) 13, 959
MaeI CTAG 2 cut(s) 6, 291
MaeIII GTNAC 2 cut(s) 293, 721
MalI GATC 4 cut(s) 84, 528, 588, 842
MboI GATC 4 cut(s) 82, 526, 586, 840
MboII GAAGA 6 cut(s) 138, 493, 496, 559, 768, 829
MfeI CAATTG 1 cut(s) 530
MhlI GDGCHC 2 cut(s) 64, 413
MluCI AATT 7 cut(s) 163, 185, 227, 239, 530, 727, 936
MlyI GAGTC 1 cut(s) 953
MmeI TCCRAC 2 cut(s) 97, 601
MroXI GAANNNNTTC 1 cut(s) 51
MslI CAYNNNNRTG 3 cut(s) 381, 632, 921
MspA1I CMGCKG 3 cut(s) 35, 151, 764
MspCI CTTAAG 1 cut(s) 395
MunI CAATTG 1 cut(s) 530
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 3 cut(s) 32, 148, 157
NdeII GATC 4 cut(s) 82, 526, 586, 840
NlaIII CATG 3 cut(s) 284, 926, 935
NlaIV GGNNCC 2 cut(s) 12, 411
NmuCI GTSAC 1 cut(s) 721
OliI CACNNNNGTG 2 cut(s) 381, 632
PagI TCATGA 1 cut(s) 931
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 2 cut(s) 550, 573
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 953
PpsI GAGTC 1 cut(s) 953
PshBI ATTAAT 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 12, 411
PspOMI GGGCCC 1 cut(s) 409
PspPI GGNCC 2 cut(s) 409, 410
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 2 cut(s) 74, 798
RsaNI GTAC 2 cut(s) 73, 797
RseI CAYNNNNRTG 3 cut(s) 381, 632, 921
SacII CCGCGG 1 cut(s) 152
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 4 cut(s) 82, 526, 586, 840
Sau96I GGNCC 2 cut(s) 409, 410
SchI GAGTC 1 cut(s) 953
SduI GDGCHC 2 cut(s) 64, 413
SfaNI GCATC 2 cut(s) 13, 959
Sfr303I CCGCGG 1 cut(s) 152
SgrBI CCGCGG 1 cut(s) 152
SmiMI CAYNNNNRTG 3 cut(s) 381, 632, 921
SmlI CTYRAG 2 cut(s) 395, 461
SmoI CTYRAG 2 cut(s) 395, 461
Sse9I AATT 7 cut(s) 163, 185, 227, 239, 530, 727, 936
SsiI CCGC 4 cut(s) 149, 151, 215, 764
SspMI CTAG 2 cut(s) 6, 291
TaaI ACNGT 1 cut(s) 982
TasI AATT 7 cut(s) 163, 185, 227, 239, 530, 727, 936
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 2 cut(s) 550, 573
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 1 cut(s) 721
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 721
TspDTI ATGAA 4 cut(s) 297, 873, 920, 948
TspRI CASTG 1 cut(s) 793
Vha464I CTTAAG 1 cut(s) 395
VspI ATTAAT 1 cut(s) 507
XapI RAATTY 5 cut(s) 185, 227, 239, 727, 936
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 2 cut(s) 6, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.