Rh4AG349700

Predicted membrane protein (DUF2232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009555
Physical Location & Seq
Forward (+)
65814173 .. 65816679
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG349700.1

Sequence Viewer

Length: 816 bp
ATGATTCCTCTGCAAATTCAGCACCTCACTCCACCCTTGTCAATTTCCCAAACTACCCCTCTCTCCTCTCTCCCTTCCCTTCCTCACCTCCTCTCTCAAAGACTCGACCTTTCCCTCAGACCCAACCCCTTCACCTTCTCCCCAATTTCACTGCCCAAACCCACCCTCAAGCTCTCAAGGGCGTCGCAAAGTCGATCGCTTTTCTTCGGAAATGAGGAGGACACCGCCGGAATTTCAACTGACCTCGACGACTTGTCCCCAAACGGCGTCGTTCACCGGAAAACATTGGCATTGGTTGAGTGCTCCATGTTTGCTGCGCTTACTGGGTTGGTGTATTTCCTCAGCAATTCTCTCGCAATTGAGAATTACTTTGGTTGCTTCTTTTCGCTGCCGATAGTGATCACCTCAATGAGATGGGGCACCGCAGCTGGAAGAAAAACTATGGTGGCAACGACTATGCTATTACTTGTCTTGTCTGGTCCGGTGAAAGCCTTAACCTATTTGCTTAAGCATGGATTAGTTGGCTTTACAATGGGAAGTTTGTGGAGGTCGGGAGCAAATTGGACTATTTCAGTTTTCTTGTGTGCAATTGTTCGAGCATTGGGTTCTGTAGGGTATGTTTTGATCACTTCTTTCTTGATCAGAGAAAACATATTTGCTCTGATCACGATAAACATTCATGCTTCTCTAACATTCATCTTCACAGCAGCAGGCATCTATACAGTTCCATCGATGGAAGCAATATATGCCTTATTTAGTATTCTGGTATCATCAACTACCCCCATTCACTATTCCCTCCCCCGGCGGCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2232

Protein Analysis

271

Amino Acids

29.57

Weight (kDa)

9.58

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2232 PF09991 125 - 235 6.7e-08 Predicted membrane protein (DUF2232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AciI CCGC 3 cut(s) 225, 423, 805
AcsI RAATTY 2 cut(s) 15, 231
AcyI GRCGYC 2 cut(s) 182, 267
AfiI CCNNNNNNNGG 1 cut(s) 801
AflII CTTAAG 1 cut(s) 506
AgsI TTSAA 1 cut(s) 237
AhdI GACNNNNNGTC 1 cut(s) 253
AluBI AGCT 2 cut(s) 172, 428
AluI AGCT 2 cut(s) 172, 428
Alw21I GWGCWC 1 cut(s) 305
AoxI GGCC 1 cut(s) 806
ApeKI GCWGC 4 cut(s) 314, 388, 425, 707
ApoI RAATTY 2 cut(s) 15, 231
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 479
AsuC2I CCSGG 1 cut(s) 802
AsuHPI GGTGA 5 cut(s) 77, 124, 266, 394, 496
AvaII GGWCC 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 422
BanI GGYRCC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 305
BbvCI CCTCAGC 1 cut(s) 341
BbvI GCAGC 4 cut(s) 301, 375, 437, 719
BccI CCATC 3 cut(s) 408, 727, 736
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BclI TGATCA 4 cut(s) 399, 624, 639, 663
BcnI CCSGG 1 cut(s) 802
BfmI CTRYAG 1 cut(s) 609
BfrI CTTAAG 1 cut(s) 506
BisI GCNGC 5 cut(s) 315, 389, 426, 708, 806
BlsI GCNGC 5 cut(s) 316, 390, 427, 709, 807
Bme1390I CCNGG 1 cut(s) 802
Bme18I GGWCC 1 cut(s) 479
BmeRI GACNNNNNGTC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 1 cut(s) 802
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 341
BpuEI CTTGAG 2 cut(s) 152, 160
BpuMI CCSGG 1 cut(s) 802
Bsa29I ATCGAT 1 cut(s) 731
BsaBI GATNNNNATC 1 cut(s) 398
BsaHI GRCGYC 2 cut(s) 182, 267
BsaJI CCNNGG 1 cut(s) 800
BsaWI WCCGGW 2 cut(s) 276, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 801
Bse1I ACTGG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 731
BseDI CCNNGG 1 cut(s) 800
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 801
BseMII CTCAG 2 cut(s) 130, 355
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 3 cut(s) 55, 80, 230
BseSI GKGCMC 1 cut(s) 422
BseXI GCAGC 4 cut(s) 301, 375, 437, 719
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 1 cut(s) 808
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 731
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 305
BsiSI CCGG 4 cut(s) 228, 277, 482, 802
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 1 cut(s) 801
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 1 cut(s) 808
Bsp1286I GDGCHC 2 cut(s) 305, 422
Bsp143I GATC 5 cut(s) 194, 399, 624, 639, 663
BspACI CCGC 3 cut(s) 225, 423, 805
BspANI GGCC 1 cut(s) 808
BspCNI CTCAG 2 cut(s) 129, 354
BspDI ATCGAT 1 cut(s) 731
BspLI GGNNCC 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 419
BspTI CTTAAG 1 cut(s) 506
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 800
BssMI GATC 5 cut(s) 194, 399, 624, 639, 663
BssNI GRCGYC 2 cut(s) 182, 267
Bst4CI ACNGT 1 cut(s) 724
BstACI GRCGYC 2 cut(s) 182, 267
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 1 cut(s) 746
BstC8I GCNNGC 1 cut(s) 712
BstDEI CTNAG 2 cut(s) 116, 341
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 5 cut(s) 197, 402, 627, 642, 666
BstMBI GATC 5 cut(s) 194, 399, 624, 639, 663
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 2 cut(s) 19, 746
BstSCI CCNGG 1 cut(s) 800
BstSFI CTRYAG 1 cut(s) 609
BstSLI GKGCMC 1 cut(s) 422
BstV1I GCAGC 4 cut(s) 301, 375, 437, 719
Bsu15I ATCGAT 1 cut(s) 731
BsuRI GGCC 1 cut(s) 808
BsuTUI ATCGAT 1 cut(s) 731
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 712
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 479
ClaI ATCGAT 1 cut(s) 731
CseI GACGC 2 cut(s) 171, 256
CviAII CATG 3 cut(s) 307, 512, 680
CviJI RGCY 5 cut(s) 172, 428, 491, 525, 808
CviKI_1 RGCY 5 cut(s) 172, 428, 491, 525, 808
DdeI CTNAG 2 cut(s) 116, 341
DpnI GATC 5 cut(s) 196, 401, 626, 641, 665
DpnII GATC 5 cut(s) 194, 399, 624, 639, 663
DriI GACNNNNNGTC 1 cut(s) 253
Eam1105I GACNNNNNGTC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 479
FaeI CATG 3 cut(s) 310, 515, 683
FaqI GGGAC 1 cut(s) 241
FatI CATG 3 cut(s) 306, 511, 679
FbaI TGATCA 4 cut(s) 399, 624, 639, 663
Fnu4HI GCNGC 5 cut(s) 315, 389, 426, 708, 806
Fsp4HI GCNGC 5 cut(s) 315, 389, 426, 708, 806
GlaI GCGC 1 cut(s) 318
GluI GCNGC 5 cut(s) 315, 389, 426, 708, 806
HaeIII GGCC 1 cut(s) 808
HapII CCGG 4 cut(s) 228, 277, 482, 802
HgaI GACGC 2 cut(s) 171, 256
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 2 cut(s) 182, 267
Hin1II CATG 3 cut(s) 310, 515, 683
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HinfI GANTC 2 cut(s) 4, 102
HpaII CCGG 4 cut(s) 228, 277, 482, 802
HphI GGTGA 5 cut(s) 77, 124, 266, 394, 496
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 4 cut(s) 119, 209, 644, 663
Hpy188III TCNNGA 3 cut(s) 552, 637, 667
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 3 cut(s) 187, 251, 272
HpyAV CCTTC 4 cut(s) 84, 89, 139, 145
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4V TGCA 2 cut(s) 13, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 746
HpyF3I CTNAG 2 cut(s) 116, 341
Hsp92I GRCGYC 2 cut(s) 182, 267
Hsp92II CATG 3 cut(s) 310, 515, 683
HspAI GCGC 1 cut(s) 317
Ksp22I TGATCA 4 cut(s) 399, 624, 639, 663
Kzo9I GATC 5 cut(s) 194, 399, 624, 639, 663
LmnI GCTCC 2 cut(s) 308, 554
LpnPI CCDG 8 cut(s) 241, 290, 309, 414, 462, 495, 696, 749
Lsp1109I GCAGC 4 cut(s) 301, 375, 437, 719
LweI GCATC 1 cut(s) 723
MalI GATC 5 cut(s) 196, 401, 626, 641, 665
MboI GATC 5 cut(s) 194, 399, 624, 639, 663
MboII GAAGA 3 cut(s) 196, 444, 691
MfeI CAATTG 2 cut(s) 357, 588
MhlI GDGCHC 2 cut(s) 305, 422
MluCI AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
MlyI GAGTC 1 cut(s) 96
MseI TTAA 2 cut(s) 494, 507
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 428
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 4 cut(s) 228, 277, 482, 802
MspR9I CCNGG 1 cut(s) 802
MunI CAATTG 2 cut(s) 357, 588
MwoI GCNNNNNNNGC 2 cut(s) 19, 746
NciI CCSGG 1 cut(s) 802
NdeII GATC 5 cut(s) 194, 399, 624, 639, 663
NlaIII CATG 3 cut(s) 310, 515, 683
NlaIV GGNNCC 1 cut(s) 421
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 5 cut(s) 316, 390, 427, 709, 807
Ple19I CGATCG 1 cut(s) 197
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 479
PvuI CGATCG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 2 cut(s) 494, 507
SatI GCNGC 5 cut(s) 315, 389, 426, 708, 806
Sau3AI GATC 5 cut(s) 194, 399, 624, 639, 663
Sau96I GGNCC 1 cut(s) 479
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 802
SduI GDGCHC 2 cut(s) 305, 422
SfaNI GCATC 1 cut(s) 723
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 3 cut(s) 167, 175, 506
SmoI CTYRAG 3 cut(s) 167, 175, 506
Sse9I AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
SsiI CCGC 3 cut(s) 225, 423, 805
StyD4I CCNGG 1 cut(s) 800
TaaI ACNGT 1 cut(s) 724
TaqI TCGA 5 cut(s) 105, 193, 246, 595, 731
TasI AATT 9 cut(s) 15, 42, 144, 231, 346, 357, 364, 559, 588
TauI GCSGC 1 cut(s) 808
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 494, 507
Tru9I TTAA 2 cut(s) 494, 507
TscAI CASTG 1 cut(s) 156
TseI GCWGC 4 cut(s) 314, 388, 425, 707
TspDTI ATGAA 2 cut(s) 668, 685
TspRI CASTG 1 cut(s) 156
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 479
XapI RAATTY 2 cut(s) 15, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.