FvH4_4g28650

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006504
Physical Location & Seq
Forward (+)
29217047 .. 29218126
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28650.t1

Sequence Viewer

Length: 1080 bp
ATGTCGATGGCGGGTTCTGCAATCGGATTTGAAGGATACGAAAAGAGGCTGGAAATTGCATTCTTTGAACCAAGCGTGTTTCGTGATCCTGAAGGAAGGGGTCTTCGTTCCCTGTCCAAATCCCAGCTGGATGAGTTTCTTGGACAAGCCGAGTGCACTATTGTCTCTTCCCTGTCAAACGATGATGTTGACTCGTATGTTCTTTCAGAGTCAAGCCTCTTCATCTACTCGTACAAGCTCATCATCAAAACTTGTGGGACGACGAAGTTGCTCCTGGCAATCCCACCTATCCTGAAGTTAGCTGAAAGCATTGGTCTCACCGTGAGATCTGTGAGGTACACTCGTGGGAGTTTCATTTTCCCTGGAGCACAATCATTCCCTCATCGAAACTTCTCCGAAGAAGTTTCTACACTTGACGCATACTTTGGGAAGCTCGGGGGAGGCAGCAGAGCTTATATGATGGGTGGGTCTGCTGGACCTCAGAAATGGCATGTGTATTCTGCATCTGCTGAACCAGCGCACTTGGTTGATCCTGTATACACCCTGGAAATGTGCATGACTGGTTTGGACAGGGCCAAGGCTTCTGTTTTTTACAAAACTGTGACAAGCTCAGCAGCTGCAATGACCAATGATTCTGGCATCCGAAAGATCCTCCCAGGTTCTGATATTTGTGATTTCGAGTTTGACCCCTGTGGTTATTCGATGAATGCCATTGAAGGAGCGGCGATTTCAACCATTCACGTTACACCAGAAGACGGTTTTAGCTATGCTAGTTTTGAATCCGCAGGATATGACTTCAATGAGTTGAACCTTAACCAGTTGGCTGAGAGGGTTTTGGCTTGTTTCAAACCGAGTGAGTTCTCTATTGCTGTCCATGCTGATGTTGCAGTCAAGTCACTCCAGCAAATTTGTTCATTGGATTTGAAGGGATACTGTCGTGAAGAGAGCAGCCACGAGGAGCTTGGAATGGGTGGTTCCATTGTTCAGTACAGGTTCCTCAAGACTGAGGCCTGTGGTTCTCCCAGATCAACTCTCAAAGGCTGTTGGAAAGAGGAAGATGAGGACTACGAATCCTCTTGA

Protein Analysis

360

Amino Acids

39.25

Weight (kDa)

4.92

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 9 - 332 6.2e-113 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 722
AccI GTMKAC 1 cut(s) 537
AciI CCGC 3 cut(s) 11, 722, 783
AclWI GGATC 3 cut(s) 80, 524, 643
AcsI RAATTY 1 cut(s) 906
AcuI CTGAAG 2 cut(s) 111, 314
AfaI GTAC 3 cut(s) 233, 338, 989
AfiI CCNNNNNNNGG 1 cut(s) 755
AgsI TTSAA 9 cut(s) 32, 68, 716, 732, 779, 799, 808, 847, 925
AjnI CCWGG 4 cut(s) 273, 361, 543, 655
AluBI AGCT 9 cut(s) 127, 238, 302, 433, 452, 609, 617, 765, 961
AluI AGCT 9 cut(s) 127, 238, 302, 433, 452, 609, 617, 765, 961
Alw21I GWGCWC 2 cut(s) 158, 370
Alw26I GTCTC 2 cut(s) 169, 320
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 3 cut(s) 80, 524, 643
AlwNI CAGNNNCTG 2 cut(s) 617, 662
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 2 cut(s) 573, 1008
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 4 cut(s) 444, 614, 617, 948
ApoI RAATTY 1 cut(s) 906
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 476, 573
AsuHPI GGTGA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 158
BaeI ACNNNNGTAYC 4 cut(s) 328, 361, 922, 955
BauI CACGAG 2 cut(s) 342, 953
BbsI GAAGAC 2 cut(s) 95, 759
Bbv12I GWGCWC 2 cut(s) 158, 370
BbvI GCAGC 4 cut(s) 456, 604, 626, 960
BccI CCATC 1 cut(s) 454
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 4 cut(s) 275, 363, 545, 657
BciVI GTATCC 2 cut(s) 29, 923
BcoDI GTCTC 2 cut(s) 169, 320
BfaI CTAG 1 cut(s) 771
BfuI GTATCC 2 cut(s) 29, 923
BglII AGATCT 1 cut(s) 326
BisI GCNGC 5 cut(s) 445, 615, 618, 723, 949
BlpI GCTNAGC 1 cut(s) 610
BlsI GCNGC 5 cut(s) 446, 616, 619, 724, 950
Bme1390I CCNGG 4 cut(s) 275, 363, 545, 657
Bme18I GGWCC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 2 cut(s) 476, 573
BmiI GGNNCC 2 cut(s) 976, 995
BmrFI CCNGG 4 cut(s) 275, 363, 545, 657
BmsI GCATC 2 cut(s) 512, 648
BpiI GAAGAC 2 cut(s) 95, 759
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 2 cut(s) 384, 884
Bpu1102I GCTNAGC 1 cut(s) 610
BpuEI CTTGAG 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 4 cut(s) 361, 543, 576, 655
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse1I ACTGG 2 cut(s) 565, 817
Bse3DI GCAATG 1 cut(s) 627
BseBI CCWGG 4 cut(s) 275, 363, 545, 657
BseDI CCNNGG 4 cut(s) 361, 543, 576, 655
BseGI GGATG 2 cut(s) 136, 639
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 4 cut(s) 494, 624, 816, 996
BseNI ACTGG 2 cut(s) 565, 817
BseRI GAGGAG 1 cut(s) 971
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 4 cut(s) 456, 604, 626, 960
BseYI CCCAGC 1 cut(s) 123
BshFI GGCC 2 cut(s) 575, 1010
BsiHKAI GWGCWC 2 cut(s) 158, 370
BsiHKCI CYCGRG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 2 cut(s) 169, 320
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 2 cut(s) 59, 712
BsnI GGCC 2 cut(s) 575, 1010
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 158, 370
Bsp143I GATC 5 cut(s) 85, 326, 529, 648, 1025
Bsp1720I GCTNAGC 1 cut(s) 610
BspACI CCGC 3 cut(s) 11, 722, 783
BspANI GGCC 2 cut(s) 575, 1010
BspCNI CTCAG 4 cut(s) 493, 623, 817, 997
BspLI GGNNCC 2 cut(s) 976, 995
BspPI GGATC 3 cut(s) 80, 524, 643
BspTNI GGTCTC 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 627
BsrI ACTGG 2 cut(s) 565, 817
BssECI CCNNGG 4 cut(s) 361, 543, 576, 655
BssMI GATC 5 cut(s) 85, 326, 529, 648, 1025
BssNAI GTATAC 1 cut(s) 538
BssSI CACGAG 2 cut(s) 342, 953
BssT1I CCWWGG 1 cut(s) 576
Bst1107I GTATAC 1 cut(s) 538
Bst2BI CACGAG 2 cut(s) 342, 953
Bst2UI CCWGG 4 cut(s) 275, 363, 545, 657
Bst4CI ACNGT 4 cut(s) 322, 601, 758, 935
Bst6I CTCTTC 3 cut(s) 172, 224, 936
BstDEI CTNAG 4 cut(s) 480, 610, 825, 1005
BstF5I GGATG 2 cut(s) 136, 639
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 5 cut(s) 88, 329, 532, 651, 1028
BstMAI GTCTC 2 cut(s) 169, 320
BstMBI GATC 5 cut(s) 85, 326, 529, 648, 1025
BstMWI GCNNNNNNNGC 4 cut(s) 17, 515, 875, 884
BstNI CCWGG 4 cut(s) 275, 363, 545, 657
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 4 cut(s) 273, 361, 543, 655
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 4 cut(s) 456, 604, 626, 960
BstV2I GAAGAC 2 cut(s) 95, 759
BstX2I RGATCY 2 cut(s) 326, 648
BstYI RGATCY 2 cut(s) 326, 648
BstZ17I GTATAC 1 cut(s) 538
BsuI GTATCC 2 cut(s) 29, 923
BsuRI GGCC 2 cut(s) 575, 1010
BtsCI GGATG 2 cut(s) 136, 639
CaiI CAGNNNCTG 2 cut(s) 617, 662
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 476, 573
CseI GACGC 1 cut(s) 425
Csp6I GTAC 3 cut(s) 232, 337, 988
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 3 cut(s) 491, 556, 875
CviQI GTAC 3 cut(s) 232, 337, 988
DdeI CTNAG 4 cut(s) 480, 610, 825, 1005
DpnI GATC 5 cut(s) 87, 328, 531, 650, 1027
DpnII GATC 5 cut(s) 85, 326, 529, 648, 1025
Eam1104I CTCTTC 3 cut(s) 172, 224, 936
EarI CTCTTC 3 cut(s) 172, 224, 936
Eco130I CCWWGG 1 cut(s) 576
Eco147I AGGCCT 1 cut(s) 1010
Eco31I GGTCTC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 2 cut(s) 111, 314
Eco88I CYCGRG 1 cut(s) 434
EcoRII CCWGG 4 cut(s) 273, 361, 543, 655
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 3 cut(s) 494, 559, 878
FaqI GGGAC 1 cut(s) 271
FatI CATG 3 cut(s) 490, 555, 874
FauI CCCGC 1 cut(s) 4
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 5 cut(s) 445, 615, 618, 723, 949
FokI GGATG 2 cut(s) 143, 626
Fsp4HI GCNGC 5 cut(s) 445, 615, 618, 723, 949
FspBI CTAG 1 cut(s) 771
GlaI GCGC 1 cut(s) 519
GluI GCNGC 5 cut(s) 445, 615, 618, 723, 949
GsaI CCCAGC 1 cut(s) 127
GsuI CTGGAG 2 cut(s) 384, 884
HaeIII GGCC 2 cut(s) 575, 1010
HgaI GACGC 1 cut(s) 425
HhaI GCGC 1 cut(s) 520
Hin1II CATG 3 cut(s) 494, 559, 878
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 5 cut(s) 191, 209, 632, 779, 1070
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 4 cut(s) 156, 190, 339, 538
Hpy188I TCNGA 6 cut(s) 26, 208, 397, 483, 644, 664
Hpy188III TCNNGA 6 cut(s) 83, 89, 292, 938, 1000, 1077
Hpy8I GTNNAC 4 cut(s) 156, 190, 339, 538
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 5 cut(s) 26, 86, 90, 710, 919
HpyCH4III ACNGT 4 cut(s) 322, 601, 758, 935
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 7 cut(s) 20, 59, 156, 503, 555, 620, 887
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 515, 875, 884
HpyF3I CTNAG 4 cut(s) 480, 610, 825, 1005
HpySE526I ACGT 1 cut(s) 741
Hsp92II CATG 3 cut(s) 494, 559, 878
HspAI GCGC 1 cut(s) 518
Kzo9I GATC 5 cut(s) 85, 326, 529, 648, 1025
LmnI GCTCC 4 cut(s) 276, 365, 719, 958
Lsp1109I GCAGC 4 cut(s) 456, 604, 626, 960
LweI GCATC 2 cut(s) 512, 648
MaeI CTAG 1 cut(s) 771
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 3 cut(s) 601, 742, 894
MalI GATC 5 cut(s) 87, 328, 531, 650, 1027
MbiI CCGCTC 1 cut(s) 722
MboI GATC 5 cut(s) 85, 326, 529, 648, 1025
MboII GAAGA 7 cut(s) 95, 159, 211, 410, 764, 953, 1067
MflI RGATCY 2 cut(s) 326, 648
MhlI GDGCHC 2 cut(s) 158, 370
MluCI AATT 2 cut(s) 54, 906
MlyI GAGTC 2 cut(s) 185, 218
MmeI TCCRAC 1 cut(s) 1025
MseI TTAA 1 cut(s) 813
MslI CAYNNNNRTG 1 cut(s) 879
MspA1I CMGCKG 2 cut(s) 127, 617
MspR9I CCNGG 4 cut(s) 275, 363, 545, 657
Mva1269I GAATGC 2 cut(s) 59, 712
MvaI CCWGG 4 cut(s) 275, 363, 545, 657
MwoI GCNNNNNNNGC 4 cut(s) 17, 515, 875, 884
NdeII GATC 5 cut(s) 85, 326, 529, 648, 1025
NlaIII CATG 3 cut(s) 494, 559, 878
NlaIV GGNNCC 2 cut(s) 976, 995
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 2 cut(s) 601, 894
NspI RCATGY 1 cut(s) 494
PceI AGGCCT 1 cut(s) 1010
PctI GAATGC 2 cut(s) 59, 712
PfeI GAWTC 3 cut(s) 632, 779, 1070
PkrI GCNGC 5 cut(s) 446, 616, 619, 724, 950
PleI GAGTC 2 cut(s) 185, 217
PpsI GAGTC 2 cut(s) 185, 217
Psp6I CCWGG 4 cut(s) 273, 361, 543, 655
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 4 cut(s) 273, 361, 543, 655
PspN4I GGNNCC 2 cut(s) 976, 995
PspPI GGNCC 2 cut(s) 476, 573
PstNI CAGNNNCTG 2 cut(s) 617, 662
PsuI RGATCY 2 cut(s) 326, 648
PvuII CAGCTG 2 cut(s) 127, 617
RsaI GTAC 3 cut(s) 233, 338, 989
RsaNI GTAC 3 cut(s) 232, 337, 988
RseI CAYNNNNRTG 1 cut(s) 879
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 5 cut(s) 445, 615, 618, 723, 949
Sau3AI GATC 5 cut(s) 85, 326, 529, 648, 1025
Sau96I GGNCC 2 cut(s) 476, 573
SchI GAGTC 2 cut(s) 185, 218
ScrFI CCNGG 4 cut(s) 275, 363, 545, 657
SduI GDGCHC 2 cut(s) 158, 370
SfaNI GCATC 2 cut(s) 512, 648
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 879
SmlI CTYRAG 1 cut(s) 998
SmoI CTYRAG 1 cut(s) 998
Sse9I AATT 2 cut(s) 54, 906
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 3 cut(s) 11, 722, 783
SspMI CTAG 1 cut(s) 771
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 4 cut(s) 273, 361, 543, 655
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 4 cut(s) 322, 601, 758, 935
TaiI ACGT 1 cut(s) 744
TaqI TCGA 4 cut(s) 5, 385, 678, 701
TasI AATT 2 cut(s) 54, 906
TatI WGTACW 1 cut(s) 987
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 3 cut(s) 632, 779, 1070
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TseFI GTSAC 2 cut(s) 601, 894
TseI GCWGC 4 cut(s) 444, 614, 617, 948
Tsp45I GTSAC 2 cut(s) 601, 894
TspDTI ATGAA 4 cut(s) 211, 343, 719, 903
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 1 cut(s) 906
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 959
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.