MD16G1057400.v1.1

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006504
Physical Location & Seq
Reverse (-)
4105994 .. 4108192
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1057400.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGCCATGGAGGGATCTGCTATTGGATTTGAAGGTTATGAAAAGAGGCTAGAAATAACATTTTTCGAGCCGAGTATCTTTCTCGACCCTGAAGGAAGGGGTCTTCGGTCTCTGTCCAAAGCTCAGATCGATGAATTTCTTGACCAAGCTGAGTGCACTATCGTCTCGTCATTGTCGAATGACGATGTTGACTCGTATGTTCTTTCTGAGTCAAGCCTCTTTATTCATCCATACAAGATGATCATCAAAACTTGTGGCACAACCAAGTTGCTCCGTGTGATCCCGACTGTCCTTCAGTTGGCTGAAACCATTTCCCTTGCTGTGAGATCTGTGAAGTACACTCGTGGGAGTTTCATTTTTCCAGGAGCACAGACATACCCTCATCGTAGCTTCTCAGAAGAAGTTTCGGTCCTTGACAGCTACTTCGGGAAGCTTGGGTCAGGAAGCAGGGCGTACGTAATGGGCAGGTCAGACGGACCTCAGAAATGGCATGTGTATTCTGCGACTGCCGAATCAGCAAGCTTGATCGAACCTGTGTACACACTCGAAATGTGCATGACTGGTTTGGACAGGGGAAAGGCTTCTGTATTCTACAAAACCCAATCGAGCTCAGCCACAGCGATGACAACCGAATCTGGTATTCGACAGATCCTTCCCGATTCCGACATTTGCGATTTCGAGTTTGACCCCTGTGGTTATTCCATGAACTCCATCGAAGGAGCTGCAATTTCGACCATTCACGTCACACCAGAAGATGGCTTCAGTTATGCAAGTTTTGAAACCGCTGGATATGATCTGAAAGATGTGAACCTGAACCAGTTGGTTGAGAGGGTATTGGTTTGTTTCCAACCGAAAGAGTTCTCCATAGCTGTGCACTCCAATGTTGCTAGCAAGTCACACCAGCAGCATTGTTCCGTGGATTTGAAGGGATACTGTCGCGAAGAGAGCAGCCACGAAGAGCTTGGACTGGGTGGAGCCATTGTCTACCAGAGGTTCCTGAAGACCGAGCGCTGCAGTTCTCCTCGATCAACTCTCAAGGGCTGCTGGAGAGAGGAAGAAGAGGAAGAAGATTATTGA

Protein Analysis

359

Amino Acids

39.81

Weight (kDa)

4.91

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 9 - 332 2.1e-116 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccB7I CCANNNNNTGG 1 cut(s) 755
AccI GTMKAC 1 cut(s) 984
AccII CGCG 1 cut(s) 939
AciI CCGC 1 cut(s) 783
AclWI GGATC 3 cut(s) 22, 274, 643
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 4 cut(s) 111, 278, 745, 1019
AfaI GTAC 3 cut(s) 338, 455, 539
AfeI AGCGCT 1 cut(s) 1010
AfiI CCNNNNNNNGG 2 cut(s) 298, 755
AgsI TTSAA 3 cut(s) 32, 779, 925
AjiI CACGTC 1 cut(s) 742
AjnI CCWGG 1 cut(s) 361
Alw21I GWGCWC 4 cut(s) 158, 370, 611, 876
Alw26I GTCTC 2 cut(s) 114, 169
Alw44I GTGCAC 2 cut(s) 154, 872
AlwI GGATC 3 cut(s) 22, 274, 643
Aor51HI AGCGCT 1 cut(s) 1010
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 2 cut(s) 154, 872
ApeKI GCWGC 5 cut(s) 722, 904, 948, 1011, 1041
ApoI RAATTY 1 cut(s) 134
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
Asp700I GAANNNNTTC 2 cut(s) 580, 857
AspLEI GCGC 1 cut(s) 1011
AspS9I GGNCC 2 cut(s) 409, 476
AsuNHI GCTAGC 1 cut(s) 887
AvaII GGWCC 2 cut(s) 409, 476
BaeGI GKGCMC 2 cut(s) 158, 876
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 611
BauI CACGAG 1 cut(s) 342
BbsI GAAGAC 2 cut(s) 95, 1007
Bbv12I GWGCWC 4 cut(s) 158, 370, 611, 876
BbvI GCAGC 5 cut(s) 709, 916, 960, 998, 1028
BccI CCATC 2 cut(s) 719, 749
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 1 cut(s) 363
BciVI GTATCC 1 cut(s) 923
BclI TGATCA 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 114, 169
BfaI CTAG 2 cut(s) 50, 888
BfmI CTRYAG 1 cut(s) 1012
BfoI RGCGCY 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 456
BfuI GTATCC 1 cut(s) 923
BglII AGATCT 1 cut(s) 326
BisI GCNGC 5 cut(s) 723, 905, 949, 1012, 1042
BlpI GCTNAGC 1 cut(s) 610
BlsI GCNGC 5 cut(s) 724, 906, 950, 1013, 1043
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 2 cut(s) 409, 476
BmgBI CACGTC 1 cut(s) 742
BmgT120I GGNCC 2 cut(s) 409, 476
BmiI GGNNCC 2 cut(s) 976, 995
BmrFI CCNGG 1 cut(s) 363
BmrI ACTGGG 1 cut(s) 977
BmtI GCTAGC 1 cut(s) 891
BmuI ACTGGG 1 cut(s) 977
BpiI GAAGAC 2 cut(s) 95, 1007
BpmI CTGGAG 1 cut(s) 1066
Bpu1102I GCTNAGC 1 cut(s) 610
BpuEI CTTGAG 1 cut(s) 1019
Bsa29I ATCGAT 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 457
BsaBI GATNNNNATC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 6, 915
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 755
Bse1I ACTGG 3 cut(s) 565, 817, 972
Bse8I GATNNNNATC 1 cut(s) 242
BseBI CCWGG 1 cut(s) 363
BseCI ATCGAT 1 cut(s) 129
BseDI CCNNGG 2 cut(s) 6, 915
BseGI GGATG 1 cut(s) 226
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 298, 755
BseMII CTCAG 6 cut(s) 137, 141, 198, 408, 494, 624
BseNI ACTGG 3 cut(s) 565, 817, 972
BseRI GAGGAG 1 cut(s) 1011
BseSI GKGCMC 2 cut(s) 158, 876
BseXI GCAGC 5 cut(s) 709, 916, 960, 998, 1028
Bsh1236I CGCG 1 cut(s) 939
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 129
BsiHKAI GWGCWC 4 cut(s) 158, 370, 611, 876
BsiWI CGTACG 1 cut(s) 453
BslI CCNNNNNNNGG 2 cut(s) 298, 755
BsmAI GTCTC 2 cut(s) 114, 169
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 114
Bsp1286I GDGCHC 4 cut(s) 158, 370, 611, 876
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
Bsp1720I GCTNAGC 1 cut(s) 610
Bsp19I CCATGG 1 cut(s) 6
Bsp68I TCGCGA 1 cut(s) 939
BspACI CCGC 1 cut(s) 783
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 6 cut(s) 136, 142, 199, 407, 493, 623
BspDI ATCGAT 1 cut(s) 129
BspFNI CGCG 1 cut(s) 939
BspLI GGNNCC 2 cut(s) 976, 995
BspMAI CTGCAG 1 cut(s) 1016
BspMI ACCTGC 1 cut(s) 456
BspOI GCTAGC 1 cut(s) 891
BspPI GGATC 3 cut(s) 22, 274, 643
BspQI GCTCTTC 1 cut(s) 951
BspTNI GGTCTC 1 cut(s) 114
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 3 cut(s) 565, 817, 972
BssECI CCNNGG 2 cut(s) 6, 915
BssMI GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 6
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 2 cut(s) 289, 935
Bst6I CTCTTC 3 cut(s) 936, 951, 1053
BstAUI TGTACA 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 520, 889
BstDEI CTNAG 6 cut(s) 123, 150, 207, 394, 480, 610
BstDSI CCRYGG 2 cut(s) 6, 915
BstF5I GGATG 1 cut(s) 226
BstFNI CGCG 1 cut(s) 939
BstH2I RGCGCY 1 cut(s) 1012
BstHHI GCGC 1 cut(s) 1011
BstKTI GATC 9 cut(s) 17, 129, 243, 282, 329, 528, 651, 796, 1028
BstMAI GTCTC 2 cut(s) 114, 169
BstMBI GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
BstMWI GCNNNNNNNGC 2 cut(s) 515, 945
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 1012
BstSLI GKGCMC 2 cut(s) 158, 876
BstSNI TACGTA 1 cut(s) 457
BstUI CGCG 1 cut(s) 939
BstV1I GCAGC 5 cut(s) 709, 916, 960, 998, 1028
BstV2I GAAGAC 2 cut(s) 95, 1007
BstX2I RGATCY 3 cut(s) 14, 326, 648
BstYI RGATCY 3 cut(s) 14, 326, 648
Bsu15I ATCGAT 1 cut(s) 129
BsuI GTATCC 1 cut(s) 923
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 129
BtgI CCRYGG 2 cut(s) 6, 915
BtgZI GCGATG 1 cut(s) 635
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 1 cut(s) 226
BtuMI TCGCGA 1 cut(s) 939
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 520, 889
CfoI GCGC 1 cut(s) 1011
Cfr13I GGNCC 2 cut(s) 409, 476
ClaI ATCGAT 1 cut(s) 129
Csp6I GTAC 3 cut(s) 337, 454, 538
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 4 cut(s) 7, 491, 556, 703
CviQI GTAC 3 cut(s) 337, 454, 538
DdeI CTNAG 6 cut(s) 123, 150, 207, 394, 480, 610
DpnI GATC 9 cut(s) 16, 128, 242, 281, 328, 527, 650, 795, 1027
DpnII GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 936, 951, 1053
EarI CTCTTC 3 cut(s) 936, 951, 1053
Ecl136II GAGCTC 1 cut(s) 609
Eco105I TACGTA 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 611
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 409, 476
Eco47III AGCGCT 1 cut(s) 1010
Eco53kI GAGCTC 1 cut(s) 609
Eco57I CTGAAG 4 cut(s) 111, 278, 745, 1019
EcoICRI GAGCTC 1 cut(s) 609
EcoRII CCWGG 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 4 cut(s) 10, 494, 559, 706
FatI CATG 4 cut(s) 6, 490, 555, 702
FbaI TGATCA 1 cut(s) 240
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 5 cut(s) 723, 905, 949, 1012, 1042
FokI GGATG 1 cut(s) 213
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 5 cut(s) 723, 905, 949, 1012, 1042
FspBI CTAG 2 cut(s) 50, 888
GlaI GCGC 1 cut(s) 1010
GluI GCNGC 5 cut(s) 723, 905, 949, 1012, 1042
GsuI CTGGAG 1 cut(s) 1066
HaeII RGCGCY 1 cut(s) 1012
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 1011
Hin1II CATG 4 cut(s) 10, 494, 559, 706
Hin6I GCGC 1 cut(s) 1009
HinP1I GCGC 1 cut(s) 1009
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 2 cut(s) 431, 520
HinfI GANTC 5 cut(s) 191, 209, 512, 632, 659
Hpy166II GTNNAC 8 cut(s) 156, 190, 339, 538, 540, 808, 874, 985
Hpy188I TCNGA 7 cut(s) 126, 208, 397, 472, 483, 664, 798
Hpy188III TCNNGA 8 cut(s) 83, 140, 283, 427, 441, 656, 938, 997
Hpy8I GTNNAC 8 cut(s) 156, 190, 339, 538, 540, 808, 874, 985
HpyAV CCTTC 7 cut(s) 26, 86, 90, 302, 662, 710, 919
HpyCH4III ACNGT 2 cut(s) 289, 935
HpyCH4IV ACGT 2 cut(s) 456, 741
HpyCH4V TGCA 6 cut(s) 156, 555, 725, 770, 874, 1014
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 945
HpyF3I CTNAG 6 cut(s) 123, 150, 207, 394, 480, 610
HpySE526I ACGT 2 cut(s) 456, 741
Hsp92II CATG 4 cut(s) 10, 494, 559, 706
HspAI GCGC 1 cut(s) 1009
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
LguI GCTCTTC 1 cut(s) 951
LmnI GCTCC 4 cut(s) 276, 365, 719, 974
Lsp1109I GCAGC 5 cut(s) 709, 916, 960, 998, 1028
MaeI CTAG 2 cut(s) 50, 888
MaeII ACGT 2 cut(s) 456, 741
MaeIII GTNAC 2 cut(s) 742, 894
MalI GATC 9 cut(s) 16, 128, 242, 281, 328, 527, 650, 795, 1027
MboI GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
MboII GAAGA 9 cut(s) 95, 410, 764, 953, 968, 1012, 1067, 1070, 1076
MflI RGATCY 3 cut(s) 14, 326, 648
MhlI GDGCHC 4 cut(s) 158, 370, 611, 876
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 134, 726
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 185, 218
MmeI TCCRAC 2 cut(s) 687, 871
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 580, 857
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 620, 869, 879
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 785
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 939
MwoI GCNNNNNNNGC 2 cut(s) 515, 945
NcoI CCATGG 1 cut(s) 6
NdeII GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
NheI GCTAGC 1 cut(s) 887
NlaIII CATG 4 cut(s) 10, 494, 559, 706
NlaIV GGNNCC 2 cut(s) 976, 995
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 742, 894
NruI TCGCGA 1 cut(s) 939
NspI RCATGY 1 cut(s) 494
PciSI GCTCTTC 1 cut(s) 951
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PdmI GAANNNNTTC 2 cut(s) 580, 857
PfeI GAWTC 3 cut(s) 512, 632, 659
Pfl23II CGTACG 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 755
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 5 cut(s) 724, 906, 950, 1013, 1043
PleI GAGTC 2 cut(s) 185, 217
PpsI GAGTC 2 cut(s) 185, 217
Ppu21I YACGTR 1 cut(s) 457
Psp124BI GAGCTC 1 cut(s) 611
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspLI CGTACG 1 cut(s) 453
PspN4I GGNNCC 2 cut(s) 976, 995
PspPI GGNCC 2 cut(s) 409, 476
PstI CTGCAG 1 cut(s) 1016
PsuI RGATCY 3 cut(s) 14, 326, 648
RruI TCGCGA 1 cut(s) 939
RsaI GTAC 3 cut(s) 338, 455, 539
RsaNI GTAC 3 cut(s) 337, 454, 538
RseI CAYNNNNRTG 3 cut(s) 620, 869, 879
SacI GAGCTC 1 cut(s) 611
SapI GCTCTTC 1 cut(s) 951
SatI GCNGC 5 cut(s) 723, 905, 949, 1012, 1042
Sau3AI GATC 9 cut(s) 14, 126, 240, 279, 326, 525, 648, 793, 1025
Sau96I GGNCC 2 cut(s) 409, 476
SchI GAGTC 2 cut(s) 185, 218
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 4 cut(s) 158, 370, 611, 876
SfcI CTRYAG 1 cut(s) 1012
SinI GGWCC 2 cut(s) 409, 476
SmiMI CAYNNNNRTG 3 cut(s) 620, 869, 879
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
SnaBI TACGTA 1 cut(s) 457
Sse9I AATT 2 cut(s) 134, 726
SsiI CCGC 1 cut(s) 783
SspMI CTAG 2 cut(s) 50, 888
SstI GAGCTC 1 cut(s) 611
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 2 cut(s) 289, 935
TaiI ACGT 2 cut(s) 459, 744
TaqII GACCGA 3 cut(s) 96, 397, 1019
TasI AATT 2 cut(s) 134, 726
TatI WGTACW 2 cut(s) 336, 537
TfiI GAWTC 3 cut(s) 512, 632, 659
TseFI GTSAC 2 cut(s) 742, 894
TseI GCWGC 5 cut(s) 722, 904, 948, 1011, 1041
Tsp45I GTSAC 2 cut(s) 742, 894
TspDTI ATGAA 5 cut(s) 54, 147, 215, 343, 719
TspGWI ACGGA 3 cut(s) 263, 489, 904
Van91I CCANNNNNTGG 1 cut(s) 755
VneI GTGCAC 2 cut(s) 154, 872
VpaK11BI GGWCC 2 cut(s) 409, 476
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 959
XmiI GTMKAC 1 cut(s) 984
XmnI GAANNNNTTC 2 cut(s) 580, 857
XspI CTAG 2 cut(s) 50, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.