Rw4G030650

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006504
Physical Location & Seq
Forward (+)
57383598 .. 57384677
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030650.1

Sequence Viewer

Length: 1080 bp
ATGTCGATGGCGGGTTCTGCAATTGGATTTGAAGGATACGAAAAGAGGCTGGAAATTGCATTTTTTGAACCAAGTATCTTTCGTGATCCCGAAGGAAGGGGTCTTCGATCCCTGTCCAAATCCCAGCTGGACGAGTTTCTTGGACAAGCTGAGTGCACTATTGTCTCATCACTGTCGAACGATGAAGTTGACTCATATGTTCTTTCAGAGTCAAGCCTCTTTGTTTACTCCTACAAGATCATCATCAAAACTTGTGGGACGACCAAGTTGCTCCTAGCAATCCCACCGATCCTGAAGTTAGCTGAAAGCATTGATCTCACCGTGAGATCTGTGAGGTATACTCGTGGGAGCTTCATTTTCCCAGGAGCACAATCATTCCCTCACCGAAACTTCTCCGAAGAAGTTTCTACTCTTGATGCTTACTTTGGGAAGCTCGGGGGAGGCAGCAGAGCTTATATGATGGGTGGGTCTGGCGGACCTCAGAAATGGCATGTGTATTCTGCTTCAGCTGAACCAGTTAGCTTGGTTGATCCTGTATACACCCTAGAAATGTGCATGACTGGTCTGGACAAGCAAAAGGCTTCTGTTTTCTACAAATCTGAGTCAAGCTCAGCAGCTGGAATGACTAATGATTCTGGAATCCGAAGGATCCTACCAGATTCTGATATTTGTGATTTCGAATTTGACCCCTGTGGTTATTCGATGAATGCCATTGAAGGAGCGGCAATTTCAACCATTCATGTCACACCAGAAGATGGTTTTAGCTATGCTAGCTTTGAATCCGCAGGATATGACTTCAATGAGTTGAATCTTAACCAGTTGACTGAGAGGGTTTTGGCTTGTTTCAAACCGAGTGAGTTCTCCATTGCTGTGCATGCTGATGTTGCAGTCAAGTCACTCCAGCAAATTTGTTCATTGGATTTGAAGGGATACTGTCGTGAAGAGAGCAGCCACGAGGAGCTTGGAATGGGTGGTTCCATTGTTCATTACAGGTTCCTCAAGACTGAGGCCTGTGGTTCTCCAAGATCAACTCTTAAAGGCTGCTGGAAAGAGGAAGATGAGGACTACGAATCCTCTTGA

Protein Analysis

359

Amino Acids

39.43

Weight (kDa)

4.81

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 9 - 332 2.1e-113 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 722
AccI GTMKAC 2 cut(s) 338, 537
AciI CCGC 4 cut(s) 11, 474, 722, 783
AclWI GGATC 6 cut(s) 80, 102, 283, 524, 643, 656
AcsI RAATTY 2 cut(s) 680, 906
AcuI CTGAAG 2 cut(s) 314, 489
AfiI CCNNNNNNNGG 2 cut(s) 96, 755
AgsI TTSAA 9 cut(s) 32, 68, 716, 732, 779, 799, 808, 847, 925
AjnI CCWGG 1 cut(s) 361
Alw21I GWGCWC 2 cut(s) 158, 370
Alw26I GTCTC 1 cut(s) 169
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 6 cut(s) 80, 102, 283, 524, 643, 656
AlwNI CAGNNNCTG 2 cut(s) 617, 662
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 1 cut(s) 1008
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 4 cut(s) 444, 614, 948, 1041
ApoI RAATTY 2 cut(s) 680, 906
AspS9I GGNCC 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 310, 374
AsuII TTCGAA 1 cut(s) 678
AsuNHI GCTAGC 1 cut(s) 770
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 158
BaeI ACNNNNGTAYC 2 cut(s) 922, 955
BamHI GGATCC 1 cut(s) 648
BauI CACGAG 2 cut(s) 342, 953
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 2 cut(s) 158, 370
BbvI GCAGC 4 cut(s) 456, 626, 960, 1028
BccI CCATC 2 cut(s) 454, 749
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 1 cut(s) 363
BciVI GTATCC 2 cut(s) 29, 923
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 3 cut(s) 275, 545, 771
BfuI GTATCC 2 cut(s) 29, 923
BglII AGATCT 1 cut(s) 326
BisI GCNGC 5 cut(s) 445, 615, 723, 949, 1042
BlpI GCTNAGC 1 cut(s) 610
BlsI GCNGC 5 cut(s) 446, 616, 724, 950, 1043
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 3 cut(s) 650, 976, 995
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 406
BmtI GCTAGC 1 cut(s) 774
BpiI GAAGAC 1 cut(s) 95
BplI GAGNNNNNCTC 4 cut(s) 325, 357, 593, 625
BpmI CTGGAG 1 cut(s) 884
Bpu1102I GCTNAGC 1 cut(s) 610
Bpu14I TTCGAA 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 983
BsaBI GATNNNNATC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 755
Bse1I ACTGG 3 cut(s) 515, 565, 817
Bse3DI GCAATG 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 242
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 361
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 96, 755
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 6 cut(s) 141, 494, 591, 624, 816, 996
BseNI ACTGG 3 cut(s) 515, 565, 817
BseRI GAGGAG 1 cut(s) 971
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 4 cut(s) 456, 626, 960, 1028
BseYI CCCAGC 1 cut(s) 123
BshFI GGCC 1 cut(s) 1010
BsiHKAI GWGCWC 2 cut(s) 158, 370
BsiHKCI CYCGRG 1 cut(s) 434
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 2 cut(s) 96, 755
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 1 cut(s) 1010
BsoBI CYCGRG 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 2 cut(s) 158, 370
Bsp143I GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
Bsp1720I GCTNAGC 1 cut(s) 610
BspACI CCGC 4 cut(s) 11, 474, 722, 783
BspANI GGCC 1 cut(s) 1010
BspCNI CTCAG 6 cut(s) 142, 493, 592, 623, 817, 997
BspLI GGNNCC 3 cut(s) 650, 976, 995
BspOI GCTAGC 1 cut(s) 774
BspPI GGATC 6 cut(s) 80, 102, 283, 524, 643, 656
BspT104I TTCGAA 1 cut(s) 678
BsrBI CCGCTC 1 cut(s) 722
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 3 cut(s) 515, 565, 817
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
BssNAI GTATAC 2 cut(s) 339, 538
BssSI CACGAG 2 cut(s) 342, 953
Bst1107I GTATAC 2 cut(s) 339, 538
Bst2BI CACGAG 2 cut(s) 342, 953
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 3 cut(s) 174, 322, 935
Bst6I CTCTTC 1 cut(s) 936
BstBI TTCGAA 1 cut(s) 678
BstC8I GCNNGC 2 cut(s) 772, 876
BstDEI CTNAG 6 cut(s) 150, 480, 600, 610, 825, 1005
BstKTI GATC 9 cut(s) 88, 110, 240, 291, 316, 329, 532, 651, 1028
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
BstMWI GCNNNNNNNGC 4 cut(s) 17, 771, 875, 884
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 2 cut(s) 494, 878
BstSCI CCNGG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 4 cut(s) 456, 626, 960, 1028
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 2 cut(s) 326, 648
BstYI RGATCY 2 cut(s) 326, 648
BstZ17I GTATAC 2 cut(s) 339, 538
BsuI GTATCC 2 cut(s) 29, 923
BsuRI GGCC 1 cut(s) 1010
BtsIMutI CAGTG 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 772, 876
CaiI CAGNNNCTG 2 cut(s) 617, 662
Cfr13I GGNCC 1 cut(s) 476
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 4 cut(s) 491, 556, 740, 875
DdeI CTNAG 6 cut(s) 150, 480, 600, 610, 825, 1005
DpnI GATC 9 cut(s) 87, 109, 239, 290, 315, 328, 531, 650, 1027
DpnII GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
Eam1104I CTCTTC 1 cut(s) 936
EarI CTCTTC 1 cut(s) 936
EciI GGCGGA 1 cut(s) 489
Eco147I AGGCCT 1 cut(s) 1010
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 2 cut(s) 314, 489
Eco88I CYCGRG 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 361
FaeI CATG 4 cut(s) 494, 559, 743, 878
FaqI GGGAC 1 cut(s) 271
FatI CATG 4 cut(s) 490, 555, 739, 874
FauI CCCGC 1 cut(s) 4
FauNDI CATATG 1 cut(s) 196
FblI GTMKAC 2 cut(s) 338, 537
Fnu4HI GCNGC 5 cut(s) 445, 615, 723, 949, 1042
Fsp4HI GCNGC 5 cut(s) 445, 615, 723, 949, 1042
FspBI CTAG 3 cut(s) 275, 545, 771
GluI GCNGC 5 cut(s) 445, 615, 723, 949, 1042
GsaI CCCAGC 1 cut(s) 127
GsuI CTGGAG 1 cut(s) 884
HaeIII GGCC 1 cut(s) 1010
Hin1II CATG 4 cut(s) 494, 559, 743, 878
HincII GTYRAC 2 cut(s) 190, 822
HindII GTYRAC 2 cut(s) 190, 822
HinfI GANTC 9 cut(s) 191, 209, 602, 632, 639, 659, 779, 808, 1070
HphI GGTGA 2 cut(s) 310, 374
Hpy166II GTNNAC 6 cut(s) 156, 190, 226, 339, 538, 822
Hpy188I TCNGA 6 cut(s) 208, 397, 483, 601, 644, 664
Hpy188III TCNNGA 9 cut(s) 83, 89, 292, 413, 566, 636, 938, 1000, 1077
Hpy8I GTNNAC 6 cut(s) 156, 190, 226, 339, 538, 822
HpyAV CCTTC 6 cut(s) 26, 86, 90, 639, 710, 919
HpyCH4III ACNGT 3 cut(s) 174, 322, 935
HpyCH4V TGCA 6 cut(s) 20, 59, 156, 555, 874, 887
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 771, 875, 884
HpyF3I CTNAG 6 cut(s) 150, 480, 600, 610, 825, 1005
Hsp92II CATG 4 cut(s) 494, 559, 743, 878
Kzo9I GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
LmnI GCTCC 5 cut(s) 276, 348, 365, 719, 958
Lsp1109I GCAGC 4 cut(s) 456, 626, 960, 1028
LweI GCATC 1 cut(s) 406
MaeI CTAG 3 cut(s) 275, 545, 771
MaeIII GTNAC 2 cut(s) 742, 894
MalI GATC 9 cut(s) 87, 109, 239, 290, 315, 328, 531, 650, 1027
MbiI CCGCTC 1 cut(s) 722
MboI GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
MboII GAAGA 5 cut(s) 95, 410, 764, 953, 1067
MfeI CAATTG 1 cut(s) 21
MflI RGATCY 2 cut(s) 326, 648
MhlI GDGCHC 2 cut(s) 158, 370
MluCI AATT 5 cut(s) 21, 54, 680, 726, 906
MlyI GAGTC 3 cut(s) 185, 218, 611
MseI TTAA 2 cut(s) 813, 1035
MslI CAYNNNNRTG 2 cut(s) 869, 879
MspA1I CMGCKG 3 cut(s) 127, 509, 617
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 1 cut(s) 363
MwoI GCNNNNNNNGC 4 cut(s) 17, 771, 875, 884
NdeI CATATG 1 cut(s) 196
NdeII GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
NheI GCTAGC 1 cut(s) 770
NlaIII CATG 4 cut(s) 494, 559, 743, 878
NlaIV GGNNCC 3 cut(s) 650, 976, 995
NmuCI GTSAC 2 cut(s) 742, 894
NspI RCATGY 2 cut(s) 494, 878
NspV TTCGAA 1 cut(s) 678
PaeI GCATGC 1 cut(s) 878
PceI AGGCCT 1 cut(s) 1010
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 6 cut(s) 632, 639, 659, 779, 808, 1070
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 5 cut(s) 446, 616, 724, 950, 1043
PleI GAGTC 3 cut(s) 185, 217, 610
PpsI GAGTC 3 cut(s) 185, 217, 610
Psp6I CCWGG 1 cut(s) 361
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 3 cut(s) 650, 976, 995
PspPI GGNCC 1 cut(s) 476
PstNI CAGNNNCTG 2 cut(s) 617, 662
PsuI RGATCY 2 cut(s) 326, 648
PvuII CAGCTG 3 cut(s) 127, 509, 617
RseI CAYNNNNRTG 2 cut(s) 869, 879
SaqAI TTAA 2 cut(s) 813, 1035
SatI GCNGC 5 cut(s) 445, 615, 723, 949, 1042
Sau3AI GATC 9 cut(s) 85, 107, 237, 288, 313, 326, 529, 648, 1025
Sau96I GGNCC 1 cut(s) 476
SchI GAGTC 3 cut(s) 185, 218, 611
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 158, 370
SfaNI GCATC 1 cut(s) 406
SfuI TTCGAA 1 cut(s) 678
SinI GGWCC 1 cut(s) 476
SmiMI CAYNNNNRTG 2 cut(s) 869, 879
SmlI CTYRAG 1 cut(s) 998
SmoI CTYRAG 1 cut(s) 998
SphI GCATGC 1 cut(s) 878
Sse9I AATT 5 cut(s) 21, 54, 680, 726, 906
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 4 cut(s) 11, 474, 722, 783
SspMI CTAG 3 cut(s) 275, 545, 771
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 3 cut(s) 174, 322, 935
TaqI TCGA 5 cut(s) 5, 106, 176, 678, 701
TasI AATT 5 cut(s) 21, 54, 680, 726, 906
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 6 cut(s) 632, 639, 659, 779, 808, 1070
Tru1I TTAA 2 cut(s) 813, 1035
Tru9I TTAA 2 cut(s) 813, 1035
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 2 cut(s) 742, 894
TseI GCWGC 4 cut(s) 444, 614, 948, 1041
Tsp45I GTSAC 2 cut(s) 742, 894
TspDTI ATGAA 6 cut(s) 198, 343, 719, 728, 903, 974
TspRI CASTG 1 cut(s) 177
Van91I CCANNNNNTGG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 2 cut(s) 680, 906
XceI RCATGY 2 cut(s) 494, 878
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 959
XmiI GTMKAC 2 cut(s) 338, 537
XspI CTAG 3 cut(s) 275, 545, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.