pycom13g04700

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006504
Physical Location & Seq
Forward (+)
3132472 .. 3133086
615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04700.1

Sequence Viewer

Length: 615 bp
ATGGGCAGGTCAGACGGACCTCAGAAGTGGCATGTGTATTCTGCGACTGCCGAATCAGCAAGCTTCGTTGAACCTGTGTACACTCTTGAAATGTGCATGACTGGTTTGGACAGGGGAAAGGCTTCTGTATTCTACAAATCTGAATCGAGCTCAGCCACAACGATGACAAACGAATCTGGTATCCGAAAAATCCTTCCAGATTCCGATATTTGTGATTTCGAGTTTGACCCCTGTGGTTATTCCATGAATTCCATCGAAGGAGCTGCAATTTCGACCATTCACGTCACACCAGAAGATGGCTTCAGTTATGCAAGTTTTGAAACTGCTGGATATGATCTGAAATGTGTAAACCTGAACCAGTTGGTTGAGAGGGTATTGGTTTGTTTCCAACCAAAAGAATTCTCCATAGCTGTGCATGCCAATGTTGCTAGCAAGTCACTCCAGCAACATTCTCTGGTTGATCTGAAGGGATACTGTCGCGAAGAGAGCAGCCACGAAGAGCTTGGACTGGGTGGAGCCATTGTCTACCAGAGGTTCCTGAAGACTGAGCGGTGTGGTTCTCCTCGATCGACTCTCAAGGGCTGCTGGAGAGAGGAAGAGGAAGAAGATTATTAA

Protein Analysis

205

Amino Acids

22.68

Weight (kDa)

4.85

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 1 - 179 2.2e-54 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AccBSI CCGCTC 1 cut(s) 550
AccI GTMKAC 1 cut(s) 525
AccII CGCG 1 cut(s) 480
AciI CCGC 1 cut(s) 550
AcsI RAATTY 2 cut(s) 247, 398
AcuI CTGAAG 3 cut(s) 286, 485, 560
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 3 cut(s) 71, 89, 320
AjiI CACGTC 1 cut(s) 283
AluBI AGCT 5 cut(s) 63, 150, 263, 410, 502
AluI AGCT 5 cut(s) 63, 150, 263, 410, 502
Alw21I GWGCWC 1 cut(s) 152
ApeKI GCWGC 3 cut(s) 263, 489, 582
ApoI RAATTY 2 cut(s) 247, 398
Asp700I GAANNNNTTC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 17
AsuNHI GCTAGC 1 cut(s) 428
AvaII GGWCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 3 cut(s) 250, 501, 569
BccI CCATC 2 cut(s) 260, 290
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciVI GTATCC 2 cut(s) 191, 464
BfaI CTAG 1 cut(s) 429
BfuI GTATCC 2 cut(s) 191, 464
BisI GCNGC 3 cut(s) 264, 490, 583
BlpI GCTNAGC 1 cut(s) 151
BlsI GCNGC 3 cut(s) 265, 491, 584
Bme18I GGWCC 1 cut(s) 17
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 517, 536
BmrI ACTGGG 1 cut(s) 518
BmtI GCTAGC 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 548
BpmI CTGGAG 2 cut(s) 425, 607
Bpu1102I GCTNAGC 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 3 cut(s) 106, 358, 513
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 3 cut(s) 35, 165, 537
BseNI ACTGG 3 cut(s) 106, 358, 513
BseRI GAGGAG 1 cut(s) 552
BseXI GCAGC 3 cut(s) 250, 501, 569
Bsh1236I CGCG 1 cut(s) 480
Bsh1285I CGRYCG 1 cut(s) 569
BsiEI CGRYCG 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 3 cut(s) 334, 460, 566
Bsp1720I GCTNAGC 1 cut(s) 151
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 1 cut(s) 550
BspCNI CTCAG 3 cut(s) 34, 164, 538
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 517, 536
BspOI GCTAGC 1 cut(s) 432
BspQI GCTCTTC 1 cut(s) 492
BsrBI CCGCTC 1 cut(s) 550
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 3 cut(s) 106, 358, 513
BssMI GATC 3 cut(s) 334, 460, 566
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 3 cut(s) 477, 492, 591
BstAUI TGTACA 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 61, 417, 430
BstDEI CTNAG 3 cut(s) 21, 151, 546
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 3 cut(s) 337, 463, 569
BstMBI GATC 3 cut(s) 334, 460, 566
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 4 cut(s) 56, 416, 425, 486
BstNSI RCATGY 2 cut(s) 35, 419
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 3 cut(s) 250, 501, 569
BstV2I GAAGAC 1 cut(s) 548
BsuI GTATCC 2 cut(s) 191, 464
BtrI CACGTC 1 cut(s) 283
BtuMI TCGCGA 1 cut(s) 480
Cac8I GCNNGC 3 cut(s) 61, 417, 430
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 79
CviAII CATG 4 cut(s) 32, 97, 244, 416
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 3 cut(s) 21, 151, 546
DpnI GATC 3 cut(s) 336, 462, 568
DpnII GATC 3 cut(s) 334, 460, 566
Eam1104I CTCTTC 3 cut(s) 477, 492, 591
EarI CTCTTC 3 cut(s) 477, 492, 591
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 17
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 3 cut(s) 286, 485, 560
EcoICRI GAGCTC 1 cut(s) 150
EcoRI GAATTC 2 cut(s) 247, 398
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 4 cut(s) 35, 100, 247, 419
FaiI YATR 7 cut(s) 33, 98, 245, 309, 333, 407, 417
FatI CATG 4 cut(s) 31, 96, 243, 415
FblI GTMKAC 1 cut(s) 525
Fnu4HI GCNGC 3 cut(s) 264, 490, 583
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 264, 490, 583
FspBI CTAG 1 cut(s) 429
GluI GCNGC 3 cut(s) 264, 490, 583
GsuI CTGGAG 2 cut(s) 425, 607
Hin1II CATG 4 cut(s) 35, 100, 247, 419
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 5 cut(s) 53, 143, 173, 200, 571
Hpy166II GTNNAC 4 cut(s) 79, 81, 349, 526
Hpy188I TCNGA 7 cut(s) 13, 24, 142, 185, 205, 339, 465
Hpy188III TCNNGA 4 cut(s) 86, 197, 479, 538
Hpy8I GTNNAC 4 cut(s) 79, 81, 349, 526
HpyAV CCTTC 3 cut(s) 203, 251, 460
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 4 cut(s) 96, 266, 311, 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 416, 425, 486
HpyF3I CTNAG 3 cut(s) 21, 151, 546
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 4 cut(s) 35, 100, 247, 419
Kzo9I GATC 3 cut(s) 334, 460, 566
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 2 cut(s) 260, 515
Lsp1109I GCAGC 3 cut(s) 250, 501, 569
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 283, 435
MalI GATC 3 cut(s) 336, 462, 568
MbiI CCGCTC 1 cut(s) 550
MboI GATC 3 cut(s) 334, 460, 566
MboII GAAGA 6 cut(s) 305, 494, 509, 553, 608, 614
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 3 cut(s) 247, 267, 398
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 6 cut(s) 30, 363, 525, 573, 586, 592
MroXI GAANNNNTTC 1 cut(s) 121
MseI TTAA 1 cut(s) 613
MslI CAYNNNNRTG 3 cut(s) 161, 410, 420
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 4 cut(s) 56, 416, 425, 486
NdeII GATC 3 cut(s) 334, 460, 566
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 4 cut(s) 35, 100, 247, 419
NlaIV GGNNCC 2 cut(s) 517, 536
NmuCI GTSAC 2 cut(s) 283, 435
NruI TCGCGA 1 cut(s) 480
NspI RCATGY 2 cut(s) 35, 419
PaeI GCATGC 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 121
PfeI GAWTC 4 cut(s) 53, 143, 173, 200
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 3 cut(s) 265, 491, 584
Ple19I CGATCG 1 cut(s) 569
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Psp124BI GAGCTC 1 cut(s) 152
PspN4I GGNNCC 2 cut(s) 517, 536
PspPI GGNCC 1 cut(s) 17
PvuI CGATCG 1 cut(s) 569
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 3 cut(s) 161, 410, 420
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 1 cut(s) 613
SatI GCNGC 3 cut(s) 264, 490, 583
Sau3AI GATC 3 cut(s) 334, 460, 566
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 1 cut(s) 565
SduI GDGCHC 1 cut(s) 152
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 3 cut(s) 161, 410, 420
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
SphI GCATGC 1 cut(s) 419
Sse9I AATT 3 cut(s) 247, 267, 398
SsiI CCGC 1 cut(s) 550
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 1 cut(s) 152
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 285
TaqI TCGA 6 cut(s) 146, 219, 255, 272, 565, 569
TasI AATT 3 cut(s) 247, 267, 398
TatI WGTACW 1 cut(s) 78
TfiI GAWTC 4 cut(s) 53, 143, 173, 200
Tru1I TTAA 1 cut(s) 613
Tru9I TTAA 1 cut(s) 613
TseFI GTSAC 2 cut(s) 283, 435
TseI GCWGC 3 cut(s) 263, 489, 582
Tsp45I GTSAC 2 cut(s) 283, 435
TspDTI ATGAA 1 cut(s) 260
TspGWI ACGGA 1 cut(s) 30
Van91I CCANNNNNTGG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 2 cut(s) 247, 398
XceI RCATGY 2 cut(s) 35, 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 121
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.