MD13G1052600.v1.1

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006504
Physical Location & Seq
Forward (+)
3728771 .. 3731492
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1052600.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCCATGGAGGGATCTGCTATTGGATTTGAAGGTTATGAAAAGAGGCTAGAAATAGCATTTTTCGAGCCGAGTATCTTTCTCGACCCTGAAGGAAGGGGTCTTCGGTCTCTGTCCAAAGCTCAGATAGATGAATTTCTTGACCAAGCTGAGTGCACCATCGTCTCGTCATTGTCGAATGACGATGTTGACTCATATGTTCTTTCTGAGTCAAGCCTCTTTATTCATCCATACAAGATGATCATCAAAACTTGTGGCACAACCAAGTTGCTCCGTGTGATCCCGACTGTCCTGCAGTTGGCTGAAACCATTTCCCTTGCTGTGAGATCTGTGAAGTACACTCGTGGGAGTTTCATTTTTCCGGGAGCACAGACGTACCCTCATCGTAGCTTCTCAGAAGAAGTTTCGGTCCTCGACAGCTACTTCGGGAAGCTTGGGTCAGGAAGCAGGGCGTATGTGATGGGCAGGTCAGACGGACCTCAGAAGTGGCATGTGTATTCTGCGACTGCCGAATCAGCAAGCTTCGTTGAACCTGTGTACACTCTTGAAATGTGCATGACTGGTTTGGACAGGGGAAAGGCTTCTGTATTCTACAAATCTGAATCGAGCTCAGCCACAACAATGACAAACGAATCTGGTATCCGAAAAATCCTTCCAGATTCCGATATTTGTGATTTCGAGTTTGACCCCTGTGGTTATTCCATGAATTCCATCGAAGGAGCTGCAATTTCGACCATTCACGTCACACCAGAAGATGGCTTCAGTTATGCAAGTTTTGAAACCGCTGGATATGATCTGAAAGATGTAAACTTGAACCAGTTGGTTGAGAGGGTATTGGTTTGTTTCCAACCAAAAGAATTCTCCATAGCTGTGCATGCCAATGTTGTTAGCAAGTCACTCCAGCAACATTCTCTCGTCGATCTGAAGGGATACTGTCGCGAAGAGAGCAGCCACGAAGAGCTTGGACTGGGTGGAGCCATTGTCTACCAGAGGTTCCTGAAGACTGAGCGGTGCGGTTCTCCTCGATCAACTCTCAAGGGCTGCTGGAGAGAGGAAGAAGAGGAAGAAGAGGAAGAAGATTATTAA

Protein Analysis

362

Amino Acids

40.19

Weight (kDa)

4.79

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 9 - 332 3.5e-116 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccB7I CCANNNNNTGG 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 1009
AccI GTMKAC 1 cut(s) 984
AccII CGCG 1 cut(s) 939
AciI CCGC 3 cut(s) 783, 1009, 1014
AclWI GGATC 2 cut(s) 22, 274
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 134, 706, 857
AcuI CTGAAG 4 cut(s) 111, 745, 944, 1019
AfaI GTAC 3 cut(s) 338, 377, 539
AfiI CCNNNNNNNGG 2 cut(s) 298, 755
AgsI TTSAA 5 cut(s) 32, 530, 548, 779, 814
AjiI CACGTC 1 cut(s) 742
Alw21I GWGCWC 3 cut(s) 158, 370, 611
Alw26I GTCTC 2 cut(s) 114, 169
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 2 cut(s) 22, 274
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 3 cut(s) 722, 948, 1041
ApoI RAATTY 3 cut(s) 134, 706, 857
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
Asp700I GAANNNNTTC 1 cut(s) 580
AspS9I GGNCC 2 cut(s) 409, 476
AsuC2I CCSGG 1 cut(s) 363
AvaII GGWCC 2 cut(s) 409, 476
BaeGI GKGCMC 1 cut(s) 158
BaeI ACNNNNGTAYC 2 cut(s) 359, 392
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 611
BauI CACGAG 1 cut(s) 342
BbsI GAAGAC 2 cut(s) 95, 1007
Bbv12I GWGCWC 3 cut(s) 158, 370, 611
BbvI GCAGC 3 cut(s) 709, 960, 1028
BccI CCATC 4 cut(s) 167, 454, 719, 749
BcgI CGANNNNNNTGC 4 cut(s) 274, 308, 704, 738
BciVI GTATCC 2 cut(s) 650, 923
BclI TGATCA 1 cut(s) 240
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 114, 169
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 456
BfuI GTATCC 2 cut(s) 650, 923
BglII AGATCT 1 cut(s) 326
BisI GCNGC 3 cut(s) 723, 949, 1042
BlpI GCTNAGC 1 cut(s) 610
BlsI GCNGC 3 cut(s) 724, 950, 1043
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 2 cut(s) 409, 476
BmgBI CACGTC 1 cut(s) 742
BmgT120I GGNCC 2 cut(s) 409, 476
BmiI GGNNCC 2 cut(s) 976, 995
BmrFI CCNGG 1 cut(s) 363
BmrI ACTGGG 1 cut(s) 977
BmuI ACTGGG 1 cut(s) 977
BpiI GAAGAC 2 cut(s) 95, 1007
BpmI CTGGAG 2 cut(s) 884, 1066
Bpu1102I GCTNAGC 1 cut(s) 610
BpuEI CTTGAG 1 cut(s) 1019
BpuMI CCSGG 1 cut(s) 363
BsaBI GATNNNNATC 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 755
Bse1I ACTGG 3 cut(s) 565, 817, 972
Bse8I GATNNNNATC 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 226
BseJI GATNNNNATC 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 298, 755
BseMII CTCAG 7 cut(s) 137, 141, 198, 408, 494, 624, 996
BseNI ACTGG 3 cut(s) 565, 817, 972
BseRI GAGGAG 1 cut(s) 1011
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 3 cut(s) 709, 960, 1028
Bsh1236I CGCG 1 cut(s) 939
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 3 cut(s) 158, 370, 611
BsiSI CCGG 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 298, 755
BsmAI GTCTC 2 cut(s) 114, 169
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 114
Bsp1286I GDGCHC 3 cut(s) 158, 370, 611
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
Bsp1720I GCTNAGC 1 cut(s) 610
Bsp19I CCATGG 1 cut(s) 6
Bsp68I TCGCGA 1 cut(s) 939
BspACI CCGC 3 cut(s) 783, 1009, 1014
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 7 cut(s) 136, 142, 199, 407, 493, 623, 997
BspFNI CGCG 1 cut(s) 939
BspLI GGNNCC 2 cut(s) 976, 995
BspMAI CTGCAG 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 2 cut(s) 22, 274
BspQI GCTCTTC 1 cut(s) 951
BspTNI GGTCTC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 1009
BsrGI TGTACA 1 cut(s) 537
BsrI ACTGG 3 cut(s) 565, 817, 972
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 6
Bst2BI CACGAG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 289, 935
Bst6I CTCTTC 4 cut(s) 936, 951, 1053, 1062
BstAUI TGTACA 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 520, 876
BstDEI CTNAG 7 cut(s) 123, 150, 207, 394, 480, 610, 1005
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 226
BstFNI CGCG 1 cut(s) 939
BstKTI GATC 7 cut(s) 17, 243, 282, 329, 796, 922, 1028
BstMAI GTCTC 2 cut(s) 114, 169
BstMBI GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
BstMWI GCNNNNNNNGC 3 cut(s) 515, 875, 945
BstNSI RCATGY 2 cut(s) 494, 878
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 293
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 1 cut(s) 939
BstV1I GCAGC 3 cut(s) 709, 960, 1028
BstV2I GAAGAC 2 cut(s) 95, 1007
BstX2I RGATCY 2 cut(s) 14, 326
BstYI RGATCY 2 cut(s) 14, 326
BsuI GTATCC 2 cut(s) 650, 923
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 6
BtrI CACGTC 1 cut(s) 742
BtsCI GGATG 1 cut(s) 226
BtuMI TCGCGA 1 cut(s) 939
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 520, 876
Cfr13I GGNCC 2 cut(s) 409, 476
Csp6I GTAC 3 cut(s) 337, 376, 538
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 5 cut(s) 7, 491, 556, 703, 875
CviQI GTAC 3 cut(s) 337, 376, 538
DdeI CTNAG 7 cut(s) 123, 150, 207, 394, 480, 610, 1005
DpnI GATC 7 cut(s) 16, 242, 281, 328, 795, 921, 1027
DpnII GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 936, 951, 1053, 1062
EarI CTCTTC 4 cut(s) 936, 951, 1053, 1062
Ecl136II GAGCTC 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 611
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 409, 476
Eco53kI GAGCTC 1 cut(s) 609
Eco57I CTGAAG 4 cut(s) 111, 745, 944, 1019
EcoICRI GAGCTC 1 cut(s) 609
EcoRI GAATTC 2 cut(s) 706, 857
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 5 cut(s) 10, 494, 559, 706, 878
FatI CATG 5 cut(s) 6, 490, 555, 702, 874
FauNDI CATATG 1 cut(s) 196
FbaI TGATCA 1 cut(s) 240
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 3 cut(s) 723, 949, 1042
FokI GGATG 1 cut(s) 213
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 723, 949, 1042
FspBI CTAG 1 cut(s) 50
GluI GCNGC 3 cut(s) 723, 949, 1042
GsuI CTGGAG 2 cut(s) 884, 1066
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 362
Hin1II CATG 5 cut(s) 10, 494, 559, 706, 878
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 2 cut(s) 431, 520
HinfI GANTC 6 cut(s) 191, 209, 512, 602, 632, 659
HpaII CCGG 1 cut(s) 362
Hpy166II GTNNAC 7 cut(s) 156, 190, 339, 538, 540, 808, 985
Hpy188III TCNNGA 9 cut(s) 83, 140, 283, 427, 441, 545, 656, 938, 997
Hpy8I GTNNAC 7 cut(s) 156, 190, 339, 538, 540, 808, 985
Hpy99I CGWCG 1 cut(s) 920
HpyAV CCTTC 6 cut(s) 26, 86, 90, 662, 710, 919
HpyCH4III ACNGT 2 cut(s) 289, 935
HpyCH4IV ACGT 2 cut(s) 374, 741
HpyCH4V TGCA 6 cut(s) 156, 295, 555, 725, 770, 874
HpyF10VI GCNNNNNNNGC 3 cut(s) 515, 875, 945
HpyF3I CTNAG 7 cut(s) 123, 150, 207, 394, 480, 610, 1005
HpySE526I ACGT 2 cut(s) 374, 741
Hsp92II CATG 5 cut(s) 10, 494, 559, 706, 878
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
LguI GCTCTTC 1 cut(s) 951
LmnI GCTCC 4 cut(s) 276, 365, 719, 974
Lsp1109I GCAGC 3 cut(s) 709, 960, 1028
MaeI CTAG 1 cut(s) 50
MaeII ACGT 2 cut(s) 374, 741
MaeIII GTNAC 2 cut(s) 742, 894
MalI GATC 7 cut(s) 16, 242, 281, 328, 795, 921, 1027
MbiI CCGCTC 1 cut(s) 1009
MboI GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
MflI RGATCY 2 cut(s) 14, 326
MhlI GDGCHC 3 cut(s) 158, 370, 611
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 134, 706, 726, 857
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 185, 218
MmeI TCCRAC 1 cut(s) 871
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 580
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 1084
MslI CAYNNNNRTG 3 cut(s) 620, 869, 879
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 939
MwoI GCNNNNNNNGC 3 cut(s) 515, 875, 945
NciI CCSGG 1 cut(s) 363
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 196
NdeII GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
NlaIII CATG 5 cut(s) 10, 494, 559, 706, 878
NlaIV GGNNCC 2 cut(s) 976, 995
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 742, 894
NruI TCGCGA 1 cut(s) 939
NspI RCATGY 2 cut(s) 494, 878
PaeI GCATGC 1 cut(s) 878
PciSI GCTCTTC 1 cut(s) 951
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PdmI GAANNNNTTC 1 cut(s) 580
PfeI GAWTC 4 cut(s) 512, 602, 632, 659
PflMI CCANNNNNTGG 1 cut(s) 755
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 3 cut(s) 724, 950, 1043
PleI GAGTC 2 cut(s) 185, 217
PpsI GAGTC 2 cut(s) 185, 217
Psp124BI GAGCTC 1 cut(s) 611
PspN4I GGNNCC 2 cut(s) 976, 995
PspPI GGNCC 2 cut(s) 409, 476
PstI CTGCAG 1 cut(s) 297
PsuI RGATCY 2 cut(s) 14, 326
RruI TCGCGA 1 cut(s) 939
RsaI GTAC 3 cut(s) 338, 377, 539
RsaNI GTAC 3 cut(s) 337, 376, 538
RseI CAYNNNNRTG 3 cut(s) 620, 869, 879
SacI GAGCTC 1 cut(s) 611
SapI GCTCTTC 1 cut(s) 951
SaqAI TTAA 1 cut(s) 1084
SatI GCNGC 3 cut(s) 723, 949, 1042
Sau3AI GATC 7 cut(s) 14, 240, 279, 326, 793, 919, 1025
Sau96I GGNCC 2 cut(s) 409, 476
SchI GAGTC 2 cut(s) 185, 218
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 3 cut(s) 158, 370, 611
SfcI CTRYAG 1 cut(s) 293
SinI GGWCC 2 cut(s) 409, 476
SmiMI CAYNNNNRTG 3 cut(s) 620, 869, 879
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
SphI GCATGC 1 cut(s) 878
Sse9I AATT 4 cut(s) 134, 706, 726, 857
SsiI CCGC 3 cut(s) 783, 1009, 1014
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 611
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 2 cut(s) 289, 935
TaiI ACGT 2 cut(s) 377, 744
TaqII GACCGA 2 cut(s) 96, 397
TasI AATT 4 cut(s) 134, 706, 726, 857
TatI WGTACW 2 cut(s) 336, 537
TfiI GAWTC 4 cut(s) 512, 602, 632, 659
Tru1I TTAA 1 cut(s) 1084
Tru9I TTAA 1 cut(s) 1084
TseFI GTSAC 2 cut(s) 742, 894
TseI GCWGC 3 cut(s) 722, 948, 1041
Tsp45I GTSAC 2 cut(s) 742, 894
TspDTI ATGAA 5 cut(s) 54, 147, 215, 343, 719
TspGWI ACGGA 2 cut(s) 263, 489
Van91I CCANNNNNTGG 1 cut(s) 755
VneI GTGCAC 1 cut(s) 154
VpaK11BI GGWCC 2 cut(s) 409, 476
XapI RAATTY 3 cut(s) 134, 706, 857
XceI RCATGY 2 cut(s) 494, 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 959
XmiI GTMKAC 1 cut(s) 984
XmnI GAANNNNTTC 1 cut(s) 580
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.