pycom16g05020

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006504
Physical Location & Seq
Reverse (-)
3236076 .. 3236693
618 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05020.1

Sequence Viewer

Length: 618 bp
ATGGGCAGGTCAGACGGACCTCAGAAATGGCATGTATATTCGGCGACTGCCGAATCAGCAAGCTTGGTCGAACCTGTGTACACACTCGAAATGTGCATGACTGGTTTGGACAGGGGAAAGGCTTCTGTATTCTACAAAACCCAATCGAGCTCAGCCACAGCGATGACAACCGAATCTGGTATCCGAAAGATCCTTCCAGATTCGGACATTTGCGATTTCGAGTTTGACCCCTGTGGGTATTCCATGAACTCCATCGAAGGAGCTGCAATTTCGACCATTCACGTCACACCAGAAGATGGCTTCAGTTATGCGAGTTTTGAAACCGCTGGATATGATCTGAAAGATGTAAACCTGAACCAGTTGGTTGAGAGGGTATTGGTTTGTTTCCAACCGAAAGAGTTCTCCCTAGCTGTGCACTCCAATGTTGCTAGCAAGTCACTCCAGCAACATTGTTCCGTGGATTTGAAGGGATACTGTCGCGAAGAGAGCAGCCACGAAGAGCTTGGACTGGGTGGAGCCATTGTCTACCATAGGTTCCTGAAGACGGAGCGCTGCAGTTCTCCTCGATCAACTCTCAAGGGCTGCTGGAGAGAGGAAGAAGAGGAAGAAGATTATTGA

Protein Analysis

206

Amino Acids

22.8

Weight (kDa)

4.86

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 1 - 179 5.1e-54 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AccI GTMKAC 1 cut(s) 525
AccII CGCG 1 cut(s) 480
AciI CCGC 1 cut(s) 324
AclWI GGATC 1 cut(s) 184
AcuI CTGAAG 2 cut(s) 286, 560
AfaI GTAC 1 cut(s) 80
AfeI AGCGCT 1 cut(s) 551
AfiI CCNNNNNNNGG 2 cut(s) 296, 544
AgsI TTSAA 2 cut(s) 320, 466
AjiI CACGTC 1 cut(s) 283
AluBI AGCT 5 cut(s) 63, 150, 263, 410, 502
AluI AGCT 5 cut(s) 63, 150, 263, 410, 502
Alw21I GWGCWC 2 cut(s) 152, 417
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 551
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 4 cut(s) 263, 489, 552, 582
Asp700I GAANNNNTTC 2 cut(s) 121, 398
AspLEI GCGC 1 cut(s) 552
AspS9I GGNCC 1 cut(s) 17
AsuNHI GCTAGC 1 cut(s) 428
AvaII GGWCC 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 2 cut(s) 152, 417
BbvI GCAGC 4 cut(s) 250, 501, 539, 569
BccI CCATC 2 cut(s) 260, 290
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciVI GTATCC 2 cut(s) 191, 464
BfaI CTAG 2 cut(s) 407, 429
BfmI CTRYAG 1 cut(s) 553
BfoI RGCGCY 1 cut(s) 553
BfuI GTATCC 2 cut(s) 191, 464
BisI GCNGC 4 cut(s) 264, 490, 553, 583
BlpI GCTNAGC 1 cut(s) 151
BlsI GCNGC 4 cut(s) 265, 491, 554, 584
Bme18I GGWCC 1 cut(s) 17
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 517, 536
BmrI ACTGGG 1 cut(s) 518
BmtI GCTAGC 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 548
BpmI CTGGAG 2 cut(s) 425, 607
Bpu1102I GCTNAGC 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 560
BsaJI CCNNGG 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 544
Bse1I ACTGG 3 cut(s) 106, 358, 513
BseDI CCNNGG 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 296, 544
BseMII CTCAG 2 cut(s) 35, 165
BseNI ACTGG 3 cut(s) 106, 358, 513
BseRI GAGGAG 1 cut(s) 552
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 4 cut(s) 250, 501, 539, 569
Bsh1236I CGCG 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 152, 417
BslI CCNNNNNNNGG 2 cut(s) 296, 544
Bsp1286I GDGCHC 2 cut(s) 152, 417
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 3 cut(s) 189, 334, 566
Bsp1720I GCTNAGC 1 cut(s) 151
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 1 cut(s) 324
BspCNI CTCAG 2 cut(s) 34, 164
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 517, 536
BspMAI CTGCAG 1 cut(s) 557
BspOI GCTAGC 1 cut(s) 432
BspPI GGATC 1 cut(s) 184
BspQI GCTCTTC 1 cut(s) 492
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 3 cut(s) 106, 358, 513
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 3 cut(s) 189, 334, 566
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 3 cut(s) 477, 492, 594
BstAUI TGTACA 1 cut(s) 78
BstC8I GCNNGC 2 cut(s) 61, 430
BstDEI CTNAG 2 cut(s) 21, 151
BstDSI CCRYGG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 480
BstH2I RGCGCY 1 cut(s) 553
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 3 cut(s) 192, 337, 569
BstMBI GATC 3 cut(s) 189, 334, 566
BstMWI GCNNNNNNNGC 2 cut(s) 56, 486
BstNSI RCATGY 1 cut(s) 35
BstSFI CTRYAG 1 cut(s) 553
BstSLI GKGCMC 1 cut(s) 417
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 4 cut(s) 250, 501, 539, 569
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
BsuI GTATCC 2 cut(s) 191, 464
BtgI CCRYGG 1 cut(s) 456
BtgZI GCGATG 1 cut(s) 176
BtrI CACGTC 1 cut(s) 283
BtuMI TCGCGA 1 cut(s) 480
Cac8I GCNNGC 2 cut(s) 61, 430
CfoI GCGC 1 cut(s) 552
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 79
CviAII CATG 3 cut(s) 32, 97, 244
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 21, 151
DpnI GATC 3 cut(s) 191, 336, 568
DpnII GATC 3 cut(s) 189, 334, 566
Eam1104I CTCTTC 3 cut(s) 477, 492, 594
EarI CTCTTC 3 cut(s) 477, 492, 594
Ecl136II GAGCTC 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 17
Eco47III AGCGCT 1 cut(s) 551
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 2 cut(s) 286, 560
EcoICRI GAGCTC 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 152
FaeI CATG 3 cut(s) 35, 100, 247
FaiI YATR 7 cut(s) 33, 37, 98, 245, 309, 333, 531
FatI CATG 3 cut(s) 31, 96, 243
FblI GTMKAC 1 cut(s) 525
Fnu4HI GCNGC 4 cut(s) 264, 490, 553, 583
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 4 cut(s) 264, 490, 553, 583
FspBI CTAG 2 cut(s) 407, 429
GlaI GCGC 1 cut(s) 551
GluI GCNGC 4 cut(s) 264, 490, 553, 583
GsuI CTGGAG 2 cut(s) 425, 607
HaeII RGCGCY 1 cut(s) 553
HhaI GCGC 1 cut(s) 552
Hin1II CATG 3 cut(s) 35, 100, 247
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 3 cut(s) 53, 173, 200
Hpy166II GTNNAC 5 cut(s) 79, 81, 349, 415, 526
Hpy188I TCNGA 5 cut(s) 13, 24, 185, 205, 339
Hpy188III TCNNGA 3 cut(s) 197, 479, 538
Hpy8I GTNNAC 5 cut(s) 79, 81, 349, 415, 526
HpyAV CCTTC 3 cut(s) 203, 251, 460
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 4 cut(s) 96, 266, 415, 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 486
HpyF3I CTNAG 2 cut(s) 21, 151
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 3 cut(s) 35, 100, 247
HspAI GCGC 1 cut(s) 550
Kzo9I GATC 3 cut(s) 189, 334, 566
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 3 cut(s) 260, 515, 547
Lsp1109I GCAGC 4 cut(s) 250, 501, 539, 569
MaeI CTAG 2 cut(s) 407, 429
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 283, 435
MalI GATC 3 cut(s) 191, 336, 568
MboI GATC 3 cut(s) 189, 334, 566
MboII GAAGA 7 cut(s) 305, 494, 509, 553, 608, 611, 617
MflI RGATCY 1 cut(s) 189
MhlI GDGCHC 2 cut(s) 152, 417
MluCI AATT 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 5 cut(s) 30, 363, 573, 586, 595
MroXI GAANNNNTTC 2 cut(s) 121, 398
MslI CAYNNNNRTG 2 cut(s) 161, 420
MspA1I CMGCKG 1 cut(s) 326
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 2 cut(s) 56, 486
NdeII GATC 3 cut(s) 189, 334, 566
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 3 cut(s) 35, 100, 247
NlaIV GGNNCC 2 cut(s) 517, 536
NmuCI GTSAC 2 cut(s) 283, 435
NruI TCGCGA 1 cut(s) 480
NspI RCATGY 1 cut(s) 35
PciSI GCTCTTC 1 cut(s) 492
PdmI GAANNNNTTC 2 cut(s) 121, 398
PfeI GAWTC 3 cut(s) 53, 173, 200
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 4 cut(s) 265, 491, 554, 584
Psp124BI GAGCTC 1 cut(s) 152
PspN4I GGNNCC 2 cut(s) 517, 536
PspPI GGNCC 1 cut(s) 17
PstI CTGCAG 1 cut(s) 557
PsuI RGATCY 1 cut(s) 189
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 2 cut(s) 161, 420
SacI GAGCTC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 492
SatI GCNGC 4 cut(s) 264, 490, 553, 583
Sau3AI GATC 3 cut(s) 189, 334, 566
Sau96I GGNCC 1 cut(s) 17
SduI GDGCHC 2 cut(s) 152, 417
SfcI CTRYAG 1 cut(s) 553
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 2 cut(s) 161, 420
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
Sse9I AATT 1 cut(s) 267
SsiI CCGC 1 cut(s) 324
SspMI CTAG 2 cut(s) 407, 429
SstI GAGCTC 1 cut(s) 152
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 285
TaqI TCGA 7 cut(s) 69, 87, 146, 219, 255, 272, 565
TasI AATT 1 cut(s) 267
TatI WGTACW 1 cut(s) 78
TfiI GAWTC 3 cut(s) 53, 173, 200
TseFI GTSAC 2 cut(s) 283, 435
TseI GCWGC 4 cut(s) 263, 489, 552, 582
Tsp45I GTSAC 2 cut(s) 283, 435
TspDTI ATGAA 1 cut(s) 260
TspGWI ACGGA 3 cut(s) 30, 445, 560
Van91I CCANNNNNTGG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 17
XceI RCATGY 1 cut(s) 35
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 525
XmnI GAANNNNTTC 2 cut(s) 121, 398
XspI CTAG 2 cut(s) 407, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.