Rorug04G0295900

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006504
Physical Location & Seq
Forward (+)
46979133 .. 46979810
678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0295900.1

Sequence Viewer

Length: 678 bp
ATGTTCACCAGGCTAATTGGGAAACCTAAAGCGGATGAATTCTCCGGTGCCCTAACCACCATGTCCTTAGAAAAGTTAACCGAGACACTTGAAGTGCTGGAGCGAAAGGAGAAAGTACTAGAGAGGAAGGCTGCTGTTGAACATGAAAAGGCCAAGGCTTTCTCCAAAGCAAAGAATAAAAGAGCTGCTATTCAGTGTTTGATTAAGAAGAAGAGGTTCGAAGAGCAAATAGAGATACTCGGAAACTCCCAACTGCGACTCCATGATCAGATGATACTGCTAGAAGGTGCGAAAGCTACCACGGAAACTCTAGATGCCTTGAGAACTGGAGCAGCTGCAATGAAGGCAATGCAGAAAGCAACAAAGATAGATGACTTAGACAAGGTCATGGATGAAATTAATGAGCAGATGGAGAACATGAAGCAGATCCAGGAAGCTTTGTCGTATCCGATTGGTGACGGCTTTGATGAGGATGAGTTGGAAGCAGAACTTGAAGAGCTCGAAGCCGCCGAGCTGGAACGAGAACTTCTTCAGCCTGTAACTACAAGCACGTCTACTTCTGCAGCTCCGGTGCAGGTCCCAGCAGGAGTTAGGCAACCCAGTACTCGTCCTGTTCCGGAGAAGCGTAGTGCTGAAGAAGATGAACTGGCTGCTTTGCAGGCTGAGATGGCACTATAA

Protein Analysis

225

Amino Acids

25.05

Weight (kDa)

4.89

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 24 - 187 5.6e-41 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 554
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 2 cut(s) 32, 507
AclWI GGATC 1 cut(s) 421
AcsI RAATTY 1 cut(s) 38
AcuI CTGAAG 2 cut(s) 515, 654
AfaI GTAC 2 cut(s) 117, 604
AgsI TTSAA 3 cut(s) 92, 140, 494
AjiI CACGTC 1 cut(s) 552
AjnI CCWGG 2 cut(s) 8, 429
AluBI AGCT 7 cut(s) 185, 296, 335, 437, 499, 514, 566
AluI AGCT 7 cut(s) 185, 296, 335, 437, 499, 514, 566
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 1 cut(s) 421
Aor13HI TCCGGA 1 cut(s) 616
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 6 cut(s) 131, 185, 332, 335, 563, 650
ApoI RAATTY 1 cut(s) 38
AseI ATTAAT 1 cut(s) 399
Asp700I GAANNNNTTC 2 cut(s) 215, 528
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 1 cut(s) 467
AsuII TTCGAA 1 cut(s) 219
AvaII GGWCC 1 cut(s) 577
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 6 cut(s) 118, 172, 322, 344, 575, 637
BccI CCATC 2 cut(s) 403, 661
BceAI ACGGC 1 cut(s) 475
BciT130I CCWGG 2 cut(s) 10, 431
BciVI GTATCC 1 cut(s) 456
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 3 cut(s) 119, 281, 311
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 565
BfuI GTATCC 1 cut(s) 456
BisI GCNGC 7 cut(s) 132, 186, 333, 336, 507, 564, 651
BlsI GCNGC 7 cut(s) 133, 187, 334, 337, 508, 565, 652
BmcAI AGTACT 2 cut(s) 117, 604
Bme1390I CCNGG 2 cut(s) 10, 431
Bme18I GGWCC 1 cut(s) 577
BmgBI CACGTC 1 cut(s) 552
BmgT120I GGNCC 1 cut(s) 577
BmiI GGNNCC 2 cut(s) 49, 579
BmrFI CCNGG 2 cut(s) 10, 431
BmrI ACTGGG 1 cut(s) 594
BmsI GCATC 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 594
BpmI CTGGAG 2 cut(s) 119, 348
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 340
BsaJI CCNNGG 2 cut(s) 153, 300
BsaWI WCCGGW 3 cut(s) 44, 568, 616
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bse1I ACTGG 3 cut(s) 331, 600, 651
Bse3DI GCAATG 2 cut(s) 345, 354
BseAI TCCGGA 1 cut(s) 616
BseBI CCWGG 2 cut(s) 10, 431
BseDI CCNNGG 2 cut(s) 153, 300
BseGI GGATG 3 cut(s) 40, 397, 478
BseMI GCAATG 2 cut(s) 345, 354
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 3 cut(s) 331, 600, 651
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 6 cut(s) 118, 172, 322, 344, 575, 637
BseYI CCCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 593
BshFI GGCC 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 3 cut(s) 45, 569, 617
BslFI GGGAC 1 cut(s) 563
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 563
BsnI GGCC 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 52, 501
Bsp13I TCCGGA 1 cut(s) 616
Bsp143I GATC 2 cut(s) 265, 426
BspACI CCGC 2 cut(s) 32, 507
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 1 cut(s) 655
BspEI TCCGGA 1 cut(s) 616
BspLI GGNNCC 2 cut(s) 49, 579
BspMAI CTGCAG 1 cut(s) 565
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 1 cut(s) 421
BspQI GCTCTTC 2 cut(s) 216, 489
BspT104I TTCGAA 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 47
BsrDI GCAATG 2 cut(s) 345, 354
BsrI ACTGG 3 cut(s) 331, 600, 651
BssECI CCNNGG 2 cut(s) 153, 300
BssMI GATC 2 cut(s) 265, 426
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 10, 431
Bst6I CTCTTC 3 cut(s) 206, 216, 489
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 660
BstDEI CTNAG 3 cut(s) 67, 376, 663
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 40, 397, 478
BstKTI GATC 2 cut(s) 268, 429
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 2 cut(s) 265, 426
BstMWI GCNNNNNNNGC 3 cut(s) 344, 659, 668
BstNI CCWGG 2 cut(s) 10, 431
BstSCI CCNGG 2 cut(s) 8, 429
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 6 cut(s) 118, 172, 322, 344, 575, 637
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 300
BtrI CACGTC 1 cut(s) 552
BtsCI GGATG 3 cut(s) 40, 397, 478
BtsIMutI CAGTG 1 cut(s) 200
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 1 cut(s) 660
Cfr13I GGNCC 1 cut(s) 577
Csp6I GTAC 2 cut(s) 116, 603
CviAII CATG 5 cut(s) 61, 143, 263, 388, 418
CviQI GTAC 2 cut(s) 116, 603
DdeI CTNAG 3 cut(s) 67, 376, 663
DpnI GATC 2 cut(s) 267, 428
DpnII GATC 2 cut(s) 265, 426
Eam1104I CTCTTC 3 cut(s) 206, 216, 489
EarI CTCTTC 3 cut(s) 206, 216, 489
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 577
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 2 cut(s) 515, 654
EcoICRI GAGCTC 1 cut(s) 499
EcoO109I RGGNCCY 1 cut(s) 577
EcoRI GAATTC 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 8, 429
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 5 cut(s) 64, 146, 266, 391, 421
FaiI YATR 6 cut(s) 62, 144, 264, 389, 419, 676
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FaqI GGGAC 1 cut(s) 563
FatI CATG 5 cut(s) 60, 142, 262, 387, 417
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 7 cut(s) 132, 186, 333, 336, 507, 564, 651
FokI GGATG 3 cut(s) 47, 404, 485
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 7 cut(s) 132, 186, 333, 336, 507, 564, 651
FspBI CTAG 3 cut(s) 119, 281, 311
GluI GCNGC 7 cut(s) 132, 186, 333, 336, 507, 564, 651
GsaI CCCAGC 1 cut(s) 584
GsuI CTGGAG 2 cut(s) 119, 348
HaeIII GGCC 1 cut(s) 152
HapII CCGG 3 cut(s) 45, 569, 617
Hin1II CATG 5 cut(s) 64, 146, 266, 391, 421
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 1 cut(s) 258
HpaI GTTAAC 1 cut(s) 78
HpaII CCGG 3 cut(s) 45, 569, 617
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 3 cut(s) 6, 78, 555
Hpy188I TCNGA 3 cut(s) 242, 270, 450
Hpy188III TCNNGA 2 cut(s) 311, 617
Hpy8I GTNNAC 3 cut(s) 6, 78, 555
HpyAV CCTTC 3 cut(s) 121, 278, 337
HpyCH4IV ACGT 1 cut(s) 551
HpyCH4V TGCA 5 cut(s) 338, 352, 563, 574, 658
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 659, 668
HpyF3I CTNAG 3 cut(s) 67, 376, 663
HpySE526I ACGT 1 cut(s) 551
Hsp92II CATG 5 cut(s) 64, 146, 266, 391, 421
Kpn2I TCCGGA 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 265
KspAI GTTAAC 1 cut(s) 78
Kzo9I GATC 2 cut(s) 265, 426
LguI GCTCTTC 2 cut(s) 216, 489
LmnI GCTCC 3 cut(s) 100, 329, 571
Lsp1109I GCAGC 6 cut(s) 118, 172, 322, 344, 575, 637
LweI GCATC 1 cut(s) 304
MaeI CTAG 3 cut(s) 119, 281, 311
MaeII ACGT 1 cut(s) 551
MaeIII GTNAC 2 cut(s) 455, 538
MalI GATC 2 cut(s) 267, 428
MboI GATC 2 cut(s) 265, 426
MboII GAAGA 7 cut(s) 220, 223, 233, 506, 521, 647, 650
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 2 cut(s) 52, 501
MluCI AATT 3 cut(s) 15, 38, 396
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 3 cut(s) 117, 207, 463
MroI TCCGGA 1 cut(s) 616
MroXI GAANNNNTTC 2 cut(s) 215, 528
MseI TTAA 3 cut(s) 77, 204, 399
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 3 cut(s) 45, 569, 617
MspR9I CCNGG 2 cut(s) 10, 431
MvaI CCWGG 2 cut(s) 10, 431
MwoI GCNNNNNNNGC 3 cut(s) 344, 659, 668
NdeII GATC 2 cut(s) 265, 426
NlaIII CATG 5 cut(s) 64, 146, 266, 391, 421
NlaIV GGNNCC 2 cut(s) 49, 579
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 455
NspV TTCGAA 1 cut(s) 219
PciSI GCTCTTC 2 cut(s) 216, 489
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PdmI GAANNNNTTC 2 cut(s) 215, 528
PflFI GACNNNGTC 1 cut(s) 383
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 7 cut(s) 133, 187, 334, 337, 508, 565, 652
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 577
PshBI ATTAAT 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 577
Psp6I CCWGG 2 cut(s) 8, 429
PspFI CCCAGC 1 cut(s) 580
PspGI CCWGG 2 cut(s) 8, 429
PspN4I GGNNCC 2 cut(s) 49, 579
PspPI GGNCC 1 cut(s) 577
PspPPI RGGWCCY 1 cut(s) 577
PstI CTGCAG 1 cut(s) 565
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 2 cut(s) 117, 604
RsaNI GTAC 2 cut(s) 116, 603
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 2 cut(s) 216, 489
SaqAI TTAA 3 cut(s) 77, 204, 399
SatI GCNGC 7 cut(s) 132, 186, 333, 336, 507, 564, 651
Sau3AI GATC 2 cut(s) 265, 426
Sau96I GGNCC 1 cut(s) 577
ScaI AGTACT 2 cut(s) 117, 604
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 2 cut(s) 10, 431
SduI GDGCHC 2 cut(s) 52, 501
SfaNI GCATC 1 cut(s) 304
SfcI CTRYAG 1 cut(s) 561
SfuI TTCGAA 1 cut(s) 219
SinI GGWCC 1 cut(s) 577
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 3 cut(s) 15, 38, 396
SsiI CCGC 2 cut(s) 32, 507
SspMI CTAG 3 cut(s) 119, 281, 311
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 2 cut(s) 8, 429
StyI CCWWGG 1 cut(s) 153
TaiI ACGT 1 cut(s) 554
TaqI TCGA 2 cut(s) 219, 501
TasI AATT 3 cut(s) 15, 38, 396
TatI WGTACW 2 cut(s) 115, 602
TauI GCSGC 1 cut(s) 509
Tru1I TTAA 3 cut(s) 77, 204, 399
Tru9I TTAA 3 cut(s) 77, 204, 399
TscAI CASTG 1 cut(s) 200
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 6 cut(s) 131, 185, 332, 335, 563, 650
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 6 cut(s) 51, 159, 356, 408, 434, 657
TspGWI ACGGA 1 cut(s) 317
TspRI CASTG 1 cut(s) 200
Tth111I GACNNNGTC 1 cut(s) 383
VpaK11BI GGWCC 1 cut(s) 577
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 1 cut(s) 38
XbaI TCTAGA 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 554
XmnI GAANNNNTTC 2 cut(s) 215, 528
XspI CTAG 3 cut(s) 119, 281, 311
ZrmI AGTACT 2 cut(s) 117, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.