FvH4_4g30570

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005702
Physical Location & Seq
Reverse (-)
30122625 .. 30125650
3026 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30570.t3

Sequence Viewer

Length: 813 bp
ATGCAGGCAGAGTATTTGAGCAATGTCATTCCTCCTTCGTCTCGCTCCACGAATTACATTGCACTCAGATTCTGTGATTTCCCTTCGGAAAATAGTAGATTTGGAGTATGGGAGAATCCAAGCCCGTGGCGTTACATGGTAGTATTCCAGTATGGATCTACTGTTCTGTTTAATGTTGAGGAACATGAAGTTCAAGCTTATCTGAATCTAGTCAAAGGGCATGCTTCTGGAATGCTACGAGAGATGGTGAAAGACGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTTGATGATATGCAGGGTGGGCCAGACTACATAGTACTTAGAACTTTGGACACAGATAGTATCCGCATTATAAGCAGTGTACTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCGGAGATAAACCGTGGCATGGAAAAAACTGGAACTTTCACTATGCATAAGAAGAAGCTTCTTCAACTCGTTGGAAAGGCCAACTCGACTCTAGCTGACGTTATTGTTAAAGTTGGTCTATTCGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACTTAAGGGAGGAGTATGAAGTTACACAACGATTTGGAAACTTGGATTTCAAGTTAAAGTTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAATCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTATAGAGAATATTATATCACTATACGAGATTGTTCGTGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

271

Amino Acids

31.11

Weight (kDa)

5.26

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 1 - 171 3.3e-34 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 394
AciI CCGC 2 cut(s) 349, 431
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 370
AfaI GTAC 2 cut(s) 321, 366
AfiI CCNNNNNNNGG 1 cut(s) 448
AflII CTTAAG 1 cut(s) 607
AgsI TTSAA 4 cut(s) 194, 422, 494, 655
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 4 cut(s) 197, 283, 487, 524
AluI AGCT 4 cut(s) 197, 283, 487, 524
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 163
AlwNI CAGNNNCTG 2 cut(s) 72, 806
AoxI GGCC 3 cut(s) 305, 370, 507
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 259
BalI TGGCCA 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 675
BbvCI CCTCAGC 1 cut(s) 279
BccI CCATC 2 cut(s) 238, 404
BciVI GTATCC 1 cut(s) 356
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 2 cut(s) 209, 521
BfmI CTRYAG 1 cut(s) 753
BfrI CTTAAG 1 cut(s) 607
BfuAI ACCTGC 1 cut(s) 394
BfuI GTATCC 1 cut(s) 356
BmcAI AGTACT 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 305
BmsI GCATC 2 cut(s) 568, 744
Bpu10I CCTNAGC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 125, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 448
Bse1I ACTGG 2 cut(s) 148, 463
Bse3DI GCAATG 2 cut(s) 28, 57
BseDI CCNNGG 2 cut(s) 125, 442
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 1 cut(s) 448
BseMI GCAATG 2 cut(s) 28, 57
BseMII CTCAG 2 cut(s) 79, 293
BseNI ACTGG 2 cut(s) 148, 463
BseRI GAGGAG 1 cut(s) 629
BshFI GGCC 3 cut(s) 307, 372, 509
BsiHKAI GWGCWC 1 cut(s) 675
BslI CCNNNNNNNGG 1 cut(s) 448
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 3 cut(s) 307, 372, 509
Bsp1286I GDGCHC 1 cut(s) 675
Bsp143I GATC 3 cut(s) 155, 557, 718
BspACI CCGC 2 cut(s) 349, 431
BspANI GGCC 3 cut(s) 307, 372, 509
BspCNI CTCAG 2 cut(s) 78, 292
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 1 cut(s) 163
BspTI CTTAAG 1 cut(s) 607
BsrDI GCAATG 2 cut(s) 28, 57
BsrI ACTGG 2 cut(s) 148, 463
BssECI CCNNGG 2 cut(s) 125, 442
BssMI GATC 3 cut(s) 155, 557, 718
Bst4CI ACNGT 2 cut(s) 163, 443
Bst6I CTCTTC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 607
BstAPI GCANNNNNTGC 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 6, 222
BstDEI CTNAG 3 cut(s) 65, 279, 323
BstDSI CCRYGG 2 cut(s) 125, 442
BstF5I GGATG 1 cut(s) 583
BstKTI GATC 3 cut(s) 158, 560, 721
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 155, 557, 718
BstMWI GCNNNNNNNGC 3 cut(s) 304, 357, 587
BstNSI RCATGY 1 cut(s) 224
BstSFI CTRYAG 1 cut(s) 753
BstX2I RGATCY 1 cut(s) 155
BstXI CCANNNNNNTGG 1 cut(s) 126
BstYI RGATCY 1 cut(s) 155
BsuI GTATCC 1 cut(s) 356
BsuRI GGCC 3 cut(s) 307, 372, 509
BtgI CCRYGG 2 cut(s) 125, 442
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 367
BveI ACCTGC 1 cut(s) 394
Cac8I GCNNGC 2 cut(s) 6, 222
CaiI CAGNNNCTG 2 cut(s) 72, 806
Cfr13I GGNCC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 320, 365
CviAII CATG 5 cut(s) 136, 185, 221, 448, 599
CviJI RGCY 9 cut(s) 123, 197, 277, 283, 307, 372, 487, 509, 524
CviKI_1 RGCY 9 cut(s) 123, 197, 277, 283, 307, 372, 487, 509, 524
CviQI GTAC 2 cut(s) 320, 365
DdeI CTNAG 3 cut(s) 65, 279, 323
DpnI GATC 3 cut(s) 157, 559, 720
DpnII GATC 3 cut(s) 155, 557, 718
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
EcoT22I ATGCAT 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 5 cut(s) 139, 188, 224, 451, 602
FalI AAGNNNNNCTT 2 cut(s) 367, 399
FatI CATG 5 cut(s) 135, 184, 220, 447, 598
FokI GGATG 1 cut(s) 590
FspBI CTAG 2 cut(s) 209, 521
HaeIII GGCC 3 cut(s) 307, 372, 509
Hin1II CATG 5 cut(s) 139, 188, 224, 451, 602
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HindIII AAGCTT 2 cut(s) 195, 485
HinfI GANTC 5 cut(s) 69, 115, 205, 517, 791
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 365, 750
Hpy188I TCNGA 5 cut(s) 68, 88, 204, 562, 718
Hpy188III TCNNGA 4 cut(s) 228, 553, 725, 788
Hpy8I GTNNAC 2 cut(s) 365, 750
HpyAV CCTTC 2 cut(s) 45, 93
HpyCH4III ACNGT 2 cut(s) 163, 443
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 8 cut(s) 4, 62, 298, 475, 581, 691, 735, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 357, 587
HpyF3I CTNAG 3 cut(s) 65, 279, 323
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 5 cut(s) 139, 188, 224, 451, 602
Kzo9I GATC 3 cut(s) 155, 557, 718
LmnI GCTCC 1 cut(s) 50
LweI GCATC 2 cut(s) 568, 744
MaeI CTAG 2 cut(s) 209, 521
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 2 cut(s) 131, 625
MalI GATC 3 cut(s) 157, 559, 720
MboI GATC 3 cut(s) 155, 557, 718
MboII GAAGA 5 cut(s) 434, 482, 493, 713, 733
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 1 cut(s) 675
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 2 cut(s) 52, 564
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 1 cut(s) 511
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 5 cut(s) 42, 172, 288, 567, 607
Mox20I TGGCCA 1 cut(s) 372
Mph1103I ATGCAT 1 cut(s) 477
MscI TGGCCA 1 cut(s) 372
MseI TTAA 4 cut(s) 171, 537, 608, 659
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 283
MspCI CTTAAG 1 cut(s) 607
Mva1269I GAATGC 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 304, 357, 587
NdeII GATC 3 cut(s) 155, 557, 718
NlaIII CATG 5 cut(s) 139, 188, 224, 451, 602
NsiI ATGCAT 1 cut(s) 477
NspI RCATGY 1 cut(s) 224
PaeI GCATGC 1 cut(s) 224
PctI GAATGC 1 cut(s) 237
PfeI GAWTC 4 cut(s) 69, 115, 205, 791
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PsiI TTATAA 1 cut(s) 356
PspPI GGNCC 1 cut(s) 305
PstNI CAGNNNCTG 2 cut(s) 72, 806
PsuI RGATCY 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 2 cut(s) 321, 366
RsaNI GTAC 2 cut(s) 320, 365
SaqAI TTAA 4 cut(s) 171, 537, 608, 659
Sau3AI GATC 3 cut(s) 155, 557, 718
Sau96I GGNCC 1 cut(s) 305
ScaI AGTACT 1 cut(s) 321
SchI GAGTC 1 cut(s) 511
SduI GDGCHC 1 cut(s) 675
SetI ASST 7 cut(s) 199, 285, 408, 489, 526, 531, 799
SfaNI GCATC 2 cut(s) 568, 744
SfcI CTRYAG 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
SphI GCATGC 1 cut(s) 224
Sse9I AATT 2 cut(s) 52, 564
SsiI CCGC 2 cut(s) 349, 431
SspI AATATT 1 cut(s) 763
SspMI CTAG 2 cut(s) 209, 521
TaaI ACNGT 2 cut(s) 163, 443
TaiI ACGT 1 cut(s) 531
TaqI TCGA 2 cut(s) 515, 552
TasI AATT 2 cut(s) 52, 564
TatI WGTACW 2 cut(s) 319, 364
TfiI GAWTC 4 cut(s) 69, 115, 205, 791
Tru1I TTAA 4 cut(s) 171, 537, 608, 659
Tru9I TTAA 4 cut(s) 171, 537, 608, 659
TscAI CASTG 1 cut(s) 367
TspDTI ATGAA 4 cut(s) 201, 615, 636, 671
TspRI CASTG 1 cut(s) 367
Vha464I CTTAAG 1 cut(s) 607
XceI RCATGY 1 cut(s) 224
XspI CTAG 2 cut(s) 209, 521
ZrmI AGTACT 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.