MD13G1066700.v1.1

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005702
Physical Location & Seq
Reverse (-)
4615143 .. 4618426
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1066700.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGGGAGGTGGAGAGCCACTGCTTCTCTTCTCCTCAGCCGCGTAGCCGCCGCCGGAGCTTCTAAATCTTCCGTTCCCGCCAAGCCTCCTTTCCATATAAGCTGCCCTTTAGCCTCCACCTCCTTCCACTTCCGCTTCCTCAATTCACGACCTTTCTCTGCAATCCCGTCTCGGATTTCCTTTGAAGCCAATGATTACGACTCTGAGCCGCCCTGCTACGCTCGGAGTCAGAGCCAAGCTAAGGACGACGAAGAAACGGGAAAAATACCTATTAAAGCCTACTTCCTTTGTACCAGTATCAATTTGAAGAGTATGCAAGCCGAGAATTTAATCAATGTCATTCCTCCTTCTTCTCGCTCATCGAATTACATTGCCCTCAGATTTTGCGATTTCCCTTCAGAAAATACTGAATTTGGAGTCTGGCGTTACATGGTCGTATTCCAGTATGGATCTGCTGTTCTGTTTAATGTTGAGGATCGTGAAGTTGATGGTTATCTGGATCTAGTTACAAGGCATGCTTCTGGATTACTTCCAGAGATGAGAAAAGACGATTATGCTGTAAAAGAAAAGCCACAGCTGGATGAGGACATGCAGGGTGGCCCAGACTACATAGTTCTTAAGACTTTGGACACTGATTCTATTCGCATTATTGGCAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCCCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGTGGAATGGAGAAAACTGGAACTTTCACAATGCACAAAAAGAAGCTCCTTCAACTTGTTGGGAAGGCTAACTCAAACTTAGCTGACGTAATTCTTAAAGTTGGTCTTTTTGAAAGATCAGAAATTGCTTGGAGGGATGCAAAGTACGCTCAAATACATGAGTACCTTCGGGAGGAGTACGAGGTTACACAACGCTTCGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTTCAAAACAGAAAGTCGGATCTTCTGGAATGGTGCATCATCTTCCTGTTGAGCATAGAAAACATTATATCACTATATGAGATTGTTCGTGATTCAGCTGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

367

Amino Acids

41.55

Weight (kDa)

5.52

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 144 - 314 1.5e-32 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 42
AciI CCGC 6 cut(s) 40, 48, 51, 78, 133, 209
AclWI GGATC 4 cut(s) 457, 483, 507, 1020
AcoI YGGCCR 1 cut(s) 664
AcsI RAATTY 3 cut(s) 325, 410, 956
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 4 cut(s) 292, 881, 899, 914
AfiI CCNNNNNNNGG 2 cut(s) 241, 907
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 7 cut(s) 185, 307, 716, 788, 848, 949, 998
AjnI CCWGG 1 cut(s) 696
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 8 cut(s) 59, 102, 239, 577, 781, 818, 1091, 1097
AluI AGCT 8 cut(s) 59, 102, 239, 577, 781, 818, 1091, 1097
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 4 cut(s) 457, 483, 507, 1020
AoxI GGCC 2 cut(s) 598, 664
ApeKI GCWGC 3 cut(s) 102, 1091, 1094
ApoI RAATTY 3 cut(s) 325, 410, 956
Asp700I GAANNNNTTC 1 cut(s) 993
AspS9I GGNCC 1 cut(s) 599
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BalI TGGCCA 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 969
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 89, 1078
BccI CCATC 2 cut(s) 482, 698
BciT130I CCWGG 1 cut(s) 698
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 1 cut(s) 1092
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 7 cut(s) 40, 48, 51, 103, 209, 1092, 1095
BlsI GCNGC 7 cut(s) 41, 49, 52, 104, 210, 1093, 1096
Bme1390I CCNGG 1 cut(s) 698
BmgT120I GGNCC 1 cut(s) 599
BmrFI CCNGG 1 cut(s) 698
BmsI GCATC 2 cut(s) 862, 1038
Bpu10I CCTNAGC 2 cut(s) 35, 240
BsaBI GATNNNNATC 2 cut(s) 492, 732
BsaJI CCNNGG 1 cut(s) 696
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 907
Bse1I ACTGG 3 cut(s) 294, 442, 757
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 2 cut(s) 492, 732
BseBI CCWGG 1 cut(s) 698
BseDI CCNNGG 1 cut(s) 696
BseGI GGATG 2 cut(s) 586, 877
BseJI GATNNNNATC 2 cut(s) 492, 732
BseLI CCNNNNNNNGG 2 cut(s) 241, 907
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 3 cut(s) 49, 195, 391
BseNI ACTGG 3 cut(s) 294, 442, 757
BseRI GAGGAG 2 cut(s) 23, 923
BseXI GCAGC 2 cut(s) 89, 1078
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 2 cut(s) 600, 666
BsiHKAI GWGCWC 1 cut(s) 969
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 241, 907
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsnI GGCC 2 cut(s) 600, 666
Bsp1286I GDGCHC 1 cut(s) 969
Bsp143I GATC 5 cut(s) 449, 475, 499, 851, 1012
BspACI CCGC 6 cut(s) 40, 48, 51, 78, 133, 209
BspANI GGCC 2 cut(s) 600, 666
BspCNI CTCAG 3 cut(s) 48, 196, 390
BspFNI CGCG 1 cut(s) 42
BspMAI CTGCAG 1 cut(s) 1096
BspPI GGATC 4 cut(s) 457, 483, 507, 1020
BspTI CTTAAG 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 3 cut(s) 294, 442, 757
BssECI CCNNGG 1 cut(s) 696
BssMI GATC 5 cut(s) 449, 475, 499, 851, 1012
Bst2UI CCWGG 1 cut(s) 698
Bst6I CTCTTC 3 cut(s) 33, 302, 711
BstAFI CTTAAG 1 cut(s) 617
BstC8I GCNNGC 2 cut(s) 318, 516
BstDEI CTNAG 5 cut(s) 35, 204, 240, 377, 814
BstENI CCTNNNNNAGG 1 cut(s) 905
BstF5I GGATG 2 cut(s) 586, 877
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 5 cut(s) 452, 478, 502, 854, 1015
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 5 cut(s) 449, 475, 499, 851, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 56, 651, 881
BstNI CCWGG 1 cut(s) 698
BstNSI RCATGY 2 cut(s) 518, 592
BstSCI CCNGG 1 cut(s) 696
BstSFI CTRYAG 1 cut(s) 1092
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 2 cut(s) 89, 1078
BstX2I RGATCY 3 cut(s) 449, 499, 1012
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 3 cut(s) 449, 499, 1012
BsuRI GGCC 2 cut(s) 600, 666
BtsCI GGATG 2 cut(s) 586, 877
BtsI GCAGTG 2 cut(s) 18, 661
BtsIMutI CAGTG 3 cut(s) 18, 630, 661
Cac8I GCNNGC 2 cut(s) 318, 516
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 4 cut(s) 291, 880, 898, 913
CviAII CATG 4 cut(s) 430, 515, 589, 893
CviQI GTAC 4 cut(s) 291, 880, 898, 913
DdeI CTNAG 5 cut(s) 35, 204, 240, 377, 814
DpnI GATC 5 cut(s) 451, 477, 501, 853, 1014
DpnII GATC 5 cut(s) 449, 475, 499, 851, 1012
EaeI YGGCCR 1 cut(s) 664
Eam1104I CTCTTC 3 cut(s) 33, 302, 711
EarI CTCTTC 3 cut(s) 33, 302, 711
Eco57I CTGAAG 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 905
EcoRII CCWGG 1 cut(s) 696
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 4 cut(s) 433, 518, 592, 896
FatI CATG 4 cut(s) 429, 514, 588, 892
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 7 cut(s) 40, 48, 51, 103, 209, 1092, 1095
FokI GGATG 2 cut(s) 593, 884
Fsp4HI GCNGC 7 cut(s) 40, 48, 51, 103, 209, 1092, 1095
FspBI CTAG 1 cut(s) 503
GluI GCNGC 7 cut(s) 40, 48, 51, 103, 209, 1092, 1095
HaeIII GGCC 2 cut(s) 600, 666
HapII CCGG 1 cut(s) 54
Hin1II CATG 4 cut(s) 433, 518, 592, 896
HinfI GANTC 5 cut(s) 200, 226, 417, 635, 1085
HpaII CCGG 1 cut(s) 54
Hpy188I TCNGA 9 cut(s) 174, 205, 225, 231, 380, 400, 856, 935, 1012
Hpy188III TCNNGA 8 cut(s) 147, 479, 497, 522, 533, 905, 1019, 1082
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 7 cut(s) 133, 357, 405, 793, 794, 911, 1004
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 9 cut(s) 161, 316, 592, 725, 769, 875, 985, 1029, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 651, 881
HpyF3I CTNAG 5 cut(s) 35, 204, 240, 377, 814
HpySE526I ACGT 1 cut(s) 822
Hsp92II CATG 4 cut(s) 433, 518, 592, 896
Kzo9I GATC 5 cut(s) 449, 475, 499, 851, 1012
LmnI GCTCC 2 cut(s) 56, 786
Lsp1109I GCAGC 2 cut(s) 89, 1078
LweI GCATC 2 cut(s) 862, 1038
MaeI CTAG 1 cut(s) 503
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 3 cut(s) 425, 505, 919
MalI GATC 5 cut(s) 451, 477, 501, 853, 1014
MboI GATC 5 cut(s) 449, 475, 499, 851, 1012
MboII GAAGA 8 cut(s) 20, 60, 263, 319, 342, 728, 1007, 1027
MflI RGATCY 3 cut(s) 449, 499, 1012
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 8 cut(s) 142, 301, 325, 364, 410, 825, 858, 956
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 3 cut(s) 194, 235, 426
MmeI TCCRAC 1 cut(s) 990
Mox20I TGGCCA 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 993
MscI TGGCCA 1 cut(s) 666
MseI TTAA 7 cut(s) 273, 329, 465, 618, 831, 953, 1099
Msp20I TGGCCA 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 577, 1091
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 698
MvaI CCWGG 1 cut(s) 698
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 56, 651, 881
NdeII GATC 5 cut(s) 449, 475, 499, 851, 1012
NlaIII CATG 4 cut(s) 433, 518, 592, 896
NmeAIII GCCGAG 1 cut(s) 346
NspI RCATGY 2 cut(s) 518, 592
PaeI GCATGC 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 2 cut(s) 635, 1085
PkrI GCNGC 7 cut(s) 41, 49, 52, 104, 210, 1093, 1096
PleI GAGTC 3 cut(s) 194, 234, 425
PpsI GAGTC 3 cut(s) 194, 234, 425
Psp6I CCWGG 1 cut(s) 696
PspGI CCWGG 1 cut(s) 696
PspPI GGNCC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 1096
PsuI RGATCY 3 cut(s) 449, 499, 1012
PvuII CAGCTG 2 cut(s) 577, 1091
RsaI GTAC 4 cut(s) 292, 881, 899, 914
RsaNI GTAC 4 cut(s) 291, 880, 898, 913
SaqAI TTAA 7 cut(s) 273, 329, 465, 618, 831, 953, 1099
SatI GCNGC 7 cut(s) 40, 48, 51, 103, 209, 1092, 1095
Sau3AI GATC 5 cut(s) 449, 475, 499, 851, 1012
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 3 cut(s) 194, 235, 426
ScrFI CCNGG 1 cut(s) 698
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 2 cut(s) 862, 1038
SfcI CTRYAG 1 cut(s) 1092
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SphI GCATGC 1 cut(s) 518
Sse9I AATT 8 cut(s) 142, 301, 325, 364, 410, 825, 858, 956
SsiI CCGC 6 cut(s) 40, 48, 51, 78, 133, 209
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 696
TaiI ACGT 1 cut(s) 825
TaqI TCGA 1 cut(s) 362
TasI AATT 8 cut(s) 142, 301, 325, 364, 410, 825, 858, 956
TauI GCSGC 4 cut(s) 42, 50, 53, 211
TfiI GAWTC 2 cut(s) 635, 1085
Tru1I TTAA 7 cut(s) 273, 329, 465, 618, 831, 953, 1099
Tru9I TTAA 7 cut(s) 273, 329, 465, 618, 831, 953, 1099
TscAI CASTG 3 cut(s) 25, 637, 661
TseI GCWGC 3 cut(s) 102, 1091, 1094
TspDTI ATGAA 1 cut(s) 965
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 3 cut(s) 25, 637, 661
Vha464I CTTAAG 1 cut(s) 617
XagI CCTNNNNNAGG 1 cut(s) 905
XapI RAATTY 3 cut(s) 325, 410, 956
XceI RCATGY 2 cut(s) 518, 592
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.