Rw0G016920

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0005702
Physical Location & Seq
Reverse (-)
604187 .. 606923
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016920.1

Sequence Viewer

Length: 1137 bp
ATGGGGAGATGGAGAGCAGCTTCTCTTCTCCTCAGGAACATAGCCATGACCACCACACCACCCAAATCTTCTTCTCTTGTTTTCAAGCCTCTCACTCTCTCACGCTCTTCTTCTTCTTCTTCTTCTTCAATCCCCACTTCCCAATTCTACTTCTTCCTCAAGTCTAGGCCTCTCTCTGCAATCCCATCTCGGGTCTCAATTGACCCGACTGATTTCGAATCCGGGCCGGCCTATTGCGCTCATGAACAACCCAATGAAGACGACGAAACCGGGAAAATCCCCATTAAGGCGTATTTCCTTTGCACCAGTATCAATTTGAAGAGCATGCAGGCCGAGTATTTAAGCAATGTCATTCCTCTTTCGTCTCGCTCAACCAATTATATTGCACTCAGATTTTGTGATTTCCCTTCGGAAAATAGTAGATTTGGAGTATGGGAGAATCCAAGCTCCTGGCGCTACATGGTAGTATTCCAGTATGGATCAACTGTTCTGTTTAATGTTGAGGAGCATGAAGTTCAGGCTTACCTAAATCTAGTCATAAGACATGCTTCTGGAATGCTACGAGAGATGGTGAAAGACGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTCGACGATATGCAGGGTGGGCCAGACTACATAGTTCTTAGAACTTTGGACACGGATAGTATCCGCATTATTGGAAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGAGGAATGGAAAAAACTGGAACTTTTACCATGCATAAAAAGAAGCTCCTTCAACTCGTTGGAAAGGCTAACTCAACTCTAGCTGATGTAATTGTCAAAGTTGGTCTATTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACCTGAGGGAGGAGTATGAGGTTACGCAGCGATTTGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAGTCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTGTAGAGAATATTATATCACTATACGAGATTGTTCGTGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

378

Amino Acids

42.99

Weight (kDa)

5.89

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 154 - 324 7.3e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 718
AccI GTMKAC 1 cut(s) 612
AciI CCGC 1 cut(s) 673
AclWI GGATC 1 cut(s) 487
AcoI YGGCCR 1 cut(s) 694
AcsI RAATTY 1 cut(s) 986
AfiI CCNNNNNNNGG 3 cut(s) 190, 286, 937
AgsI TTSAA 6 cut(s) 85, 129, 319, 746, 818, 979
AjnI CCWGG 1 cut(s) 449
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
AluBI AGCT 5 cut(s) 20, 447, 607, 811, 848
AluI AGCT 5 cut(s) 20, 447, 607, 811, 848
Alw21I GWGCWC 1 cut(s) 999
Alw26I GTCTC 2 cut(s) 199, 369
AlwI GGATC 1 cut(s) 487
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 6 cut(s) 167, 224, 228, 330, 629, 694
ApeKI GCWGC 2 cut(s) 17, 955
ApoI RAATTY 1 cut(s) 986
AspLEI GCGC 2 cut(s) 239, 456
AspS9I GGNCC 2 cut(s) 224, 629
AsuC2I CCSGG 2 cut(s) 223, 271
AsuHPI GGTGA 1 cut(s) 583
AsuII TTCGAA 1 cut(s) 216
AvaI CYCGRG 1 cut(s) 189
AxyI CCTNAGG 2 cut(s) 32, 932
BalI TGGCCA 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 999
BbvCI CCTCAGC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 29, 967
BccI CCATC 4 cut(s) 3, 193, 562, 728
BciT130I CCWGG 1 cut(s) 451
BciVI GTATCC 1 cut(s) 680
BcnI CCSGG 2 cut(s) 223, 271
BcoDI GTCTC 2 cut(s) 199, 369
BfaI CTAG 3 cut(s) 165, 533, 845
BfmI CTRYAG 1 cut(s) 1077
BfoI RGCGCY 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 718
BfuI GTATCC 1 cut(s) 680
BisI GCNGC 2 cut(s) 18, 956
BlsI GCNGC 2 cut(s) 19, 957
Bme1390I CCNGG 3 cut(s) 223, 271, 451
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 224, 629
BmrFI CCNGG 3 cut(s) 223, 271, 451
BmsI GCATC 2 cut(s) 892, 1068
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 603
Bpu14I TTCGAA 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 2 cut(s) 223, 271
BsaBI GATNNNNATC 1 cut(s) 762
BsaI GGTCTC 1 cut(s) 199
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 286, 937
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 3 cut(s) 306, 472, 787
Bse21I CCTNAGG 2 cut(s) 32, 932
Bse3DI GCAATG 1 cut(s) 352
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 1 cut(s) 451
BseGI GGATG 1 cut(s) 907
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 3 cut(s) 190, 286, 937
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 4 cut(s) 46, 403, 617, 923
BseNI ACTGG 3 cut(s) 306, 472, 787
BseRI GAGGAG 3 cut(s) 20, 518, 953
BseXI GCAGC 2 cut(s) 29, 967
BshFI GGCC 6 cut(s) 169, 226, 230, 332, 631, 696
BsiHKAI GWGCWC 1 cut(s) 999
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 3 cut(s) 222, 227, 270
BslI CCNNNNNNNGG 3 cut(s) 190, 286, 937
BsmAI GTCTC 2 cut(s) 199, 369
BsmBI CGTCTC 1 cut(s) 369
BsmI GAATGC 1 cut(s) 561
BsnI GGCC 6 cut(s) 169, 226, 230, 332, 631, 696
Bso31I GGTCTC 1 cut(s) 199
BsoBI CYCGRG 1 cut(s) 189
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 999
Bsp143I GATC 3 cut(s) 479, 881, 1042
BspACI CCGC 1 cut(s) 673
BspANI GGCC 6 cut(s) 169, 226, 230, 332, 631, 696
BspCNI CTCAG 4 cut(s) 45, 402, 616, 924
BspHI TCATGA 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 718
BspPI GGATC 1 cut(s) 487
BspQI GCTCTTC 2 cut(s) 112, 314
BspT104I TTCGAA 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 352
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 3 cut(s) 306, 472, 787
BssAI RCCGGY 1 cut(s) 226
BssMI GATC 3 cut(s) 479, 881, 1042
Bst2UI CCWGG 1 cut(s) 451
Bst4CI ACNGT 2 cut(s) 487, 1078
Bst6I CTCTTC 4 cut(s) 30, 112, 314, 741
BstAPI GCANNNNNTGC 1 cut(s) 911
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 228, 326, 330
BstDEI CTNAG 5 cut(s) 32, 389, 603, 647, 932
BstENI CCTNNNNNAGG 1 cut(s) 935
BstF5I GGATG 1 cut(s) 907
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 2 cut(s) 239, 456
BstKTI GATC 3 cut(s) 482, 884, 1045
BstMAI GTCTC 2 cut(s) 199, 369
BstMBI GATC 3 cut(s) 479, 881, 1042
BstMWI GCNNNNNNNGC 4 cut(s) 236, 453, 628, 911
BstNI CCWGG 1 cut(s) 451
BstNSI RCATGY 2 cut(s) 328, 548
BstSCI CCNGG 3 cut(s) 221, 269, 449
BstSFI CTRYAG 1 cut(s) 1077
BstV1I GCAGC 2 cut(s) 29, 967
BstV2I GAAGAC 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 450
Bsu36I CCTNAGG 2 cut(s) 32, 932
BsuI GTATCC 1 cut(s) 680
BsuRI GGCC 6 cut(s) 169, 226, 230, 332, 631, 696
BtsCI GGATG 1 cut(s) 907
BveI ACCTGC 1 cut(s) 718
Cac8I GCNNGC 3 cut(s) 228, 326, 330
CaiI CAGNNNCTG 1 cut(s) 1130
CciI TCATGA 1 cut(s) 241
CfoI GCGC 2 cut(s) 239, 456
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 224, 629
CviAII CATG 8 cut(s) 46, 242, 325, 460, 509, 545, 796, 923
DdeI CTNAG 5 cut(s) 32, 389, 603, 647, 932
DpnI GATC 3 cut(s) 481, 883, 1044
DpnII GATC 3 cut(s) 479, 881, 1042
EaeI YGGCCR 1 cut(s) 694
Eam1104I CTCTTC 4 cut(s) 30, 112, 314, 741
EarI CTCTTC 4 cut(s) 30, 112, 314, 741
Eco147I AGGCCT 1 cut(s) 169
Eco31I GGTCTC 1 cut(s) 199
Eco81I CCTNAGG 2 cut(s) 32, 932
Eco88I CYCGRG 1 cut(s) 189
EcoNI CCTNNNNNAGG 1 cut(s) 935
EcoRII CCWGG 1 cut(s) 449
EcoT22I ATGCAT 1 cut(s) 801
Esp3I CGTCTC 1 cut(s) 369
FaeI CATG 8 cut(s) 49, 245, 328, 463, 512, 548, 799, 926
FalI AAGNNNNNCTT 4 cut(s) 532, 564, 691, 723
FatI CATG 8 cut(s) 45, 241, 324, 459, 508, 544, 795, 922
FblI GTMKAC 1 cut(s) 612
Fnu4HI GCNGC 2 cut(s) 18, 956
FokI GGATG 1 cut(s) 914
FseI GGCCGGCC 1 cut(s) 230
Fsp4HI GCNGC 2 cut(s) 18, 956
FspBI CTAG 3 cut(s) 165, 533, 845
GlaI GCGC 2 cut(s) 238, 455
GluI GCNGC 2 cut(s) 18, 956
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 6 cut(s) 169, 226, 230, 332, 631, 696
HapII CCGG 3 cut(s) 222, 227, 270
HhaI GCGC 2 cut(s) 239, 456
Hin1II CATG 8 cut(s) 49, 245, 328, 463, 512, 548, 799, 926
Hin6I GCGC 2 cut(s) 237, 454
HinP1I GCGC 2 cut(s) 237, 454
HincII GTYRAC 2 cut(s) 613, 1074
HindII GTYRAC 2 cut(s) 613, 1074
HinfI GANTC 3 cut(s) 218, 439, 1115
HpaII CCGG 3 cut(s) 222, 227, 270
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 2 cut(s) 613, 1074
Hpy188I TCNGA 4 cut(s) 392, 412, 886, 1042
Hpy188III TCNNGA 5 cut(s) 34, 242, 552, 1049, 1112
Hpy8I GTNNAC 2 cut(s) 613, 1074
Hpy99I CGWCG 2 cut(s) 266, 617
HpyAV CCTTC 2 cut(s) 417, 824
HpyCH4III ACNGT 2 cut(s) 487, 1078
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 453, 628, 911
HpyF3I CTNAG 5 cut(s) 32, 389, 603, 647, 932
Hsp92II CATG 8 cut(s) 49, 245, 328, 463, 512, 548, 799, 926
HspAI GCGC 2 cut(s) 237, 454
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Kzo9I GATC 3 cut(s) 479, 881, 1042
LguI GCTCTTC 2 cut(s) 112, 314
LmnI GCTCC 3 cut(s) 452, 505, 816
Lsp1109I GCAGC 2 cut(s) 29, 967
LweI GCATC 2 cut(s) 892, 1068
MaeI CTAG 3 cut(s) 165, 533, 845
MaeIII GTNAC 1 cut(s) 949
MalI GATC 3 cut(s) 481, 883, 1044
MboI GATC 3 cut(s) 479, 881, 1042
MfeI CAATTG 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 999
MlsI TGGCCA 1 cut(s) 696
MluCI AATT 7 cut(s) 143, 198, 313, 376, 855, 888, 986
MluNI TGGCCA 1 cut(s) 696
MmeI TCCRAC 1 cut(s) 805
Mox20I TGGCCA 1 cut(s) 696
Mph1103I ATGCAT 1 cut(s) 801
MroNI GCCGGC 1 cut(s) 226
MscI TGGCCA 1 cut(s) 696
MseI TTAA 4 cut(s) 285, 341, 495, 983
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 696
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 3 cut(s) 222, 227, 270
MspR9I CCNGG 3 cut(s) 223, 271, 451
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 561
MvaI CCWGG 1 cut(s) 451
MwoI GCNNNNNNNGC 4 cut(s) 236, 453, 628, 911
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 2 cut(s) 223, 271
NdeII GATC 3 cut(s) 479, 881, 1042
NgoMIV GCCGGC 1 cut(s) 226
NlaIII CATG 8 cut(s) 49, 245, 328, 463, 512, 548, 799, 926
NmeAIII GCCGAG 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 801
NspI RCATGY 2 cut(s) 328, 548
NspV TTCGAA 1 cut(s) 216
PaeI GCATGC 1 cut(s) 328
PagI TCATGA 1 cut(s) 241
PceI AGGCCT 1 cut(s) 169
PciSI GCTCTTC 2 cut(s) 112, 314
PctI GAATGC 1 cut(s) 561
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 3 cut(s) 218, 439, 1115
PkrI GCNGC 2 cut(s) 19, 957
Psp6I CCWGG 1 cut(s) 449
PspGI CCWGG 1 cut(s) 449
PspPI GGNCC 2 cut(s) 224, 629
PstNI CAGNNNCTG 1 cut(s) 1130
PvuII CAGCTG 1 cut(s) 607
RigI GGCCGGCC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 44
SalI GTCGAC 1 cut(s) 611
SapI GCTCTTC 2 cut(s) 112, 314
SaqAI TTAA 4 cut(s) 285, 341, 495, 983
SatI GCNGC 2 cut(s) 18, 956
Sau3AI GATC 3 cut(s) 479, 881, 1042
Sau96I GGNCC 2 cut(s) 224, 629
ScrFI CCNGG 3 cut(s) 223, 271, 451
SduI GDGCHC 1 cut(s) 999
SfaNI GCATC 2 cut(s) 892, 1068
SfcI CTRYAG 1 cut(s) 1077
SfuI TTCGAA 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SphI GCATGC 1 cut(s) 328
Sse9I AATT 7 cut(s) 143, 198, 313, 376, 855, 888, 986
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 1 cut(s) 673
SspI AATATT 1 cut(s) 1087
SspMI CTAG 3 cut(s) 165, 533, 845
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 3 cut(s) 221, 269, 449
TaaI ACNGT 2 cut(s) 487, 1078
TaqI TCGA 3 cut(s) 216, 612, 766
TasI AATT 7 cut(s) 143, 198, 313, 376, 855, 888, 986
TfiI GAWTC 3 cut(s) 218, 439, 1115
Tru1I TTAA 4 cut(s) 285, 341, 495, 983
Tru9I TTAA 4 cut(s) 285, 341, 495, 983
TseI GCWGC 2 cut(s) 17, 955
TspDTI ATGAA 5 cut(s) 258, 270, 525, 939, 995
TspGWI ACGGA 1 cut(s) 677
XagI CCTNNNNNAGG 1 cut(s) 935
XapI RAATTY 1 cut(s) 986
XceI RCATGY 2 cut(s) 328, 548
XmiI GTMKAC 1 cut(s) 612
XspI CTAG 3 cut(s) 165, 533, 845
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.