Rorug04G0314800

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005702
Physical Location & Seq
Reverse (-)
48967971 .. 48969998
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0314800.1

Sequence Viewer

Length: 765 bp
ATGGATGTCCTATTCGAAGCAAATCAGCCTCTTGGCCAATATTACATGGCTGCTCGGATATATTGGACTGATGATGCTGAAGTTGATAATTTTGACCATGCTAATGCTACTGCGATCTTTGAATACAATGGCAACTATACCTGTCCAACATCTCCTTCTTTTCCGAATTCCGAACCAAGAAATCGCTACAAGCCTAAACAATATAAGTTGGGCAAACCCGGAAACATAAGCGGAGTTTATTCAACAGATTTCCCAGACCAACCACTTTCTTCCTATAACTTCACAGATGTAACTTTTGATCCTGATATTGTGTTCACAGACCAAGCGAGAAAGGTAAAGGTCTTGAATTACAACGAATCAGTTGAGATTGTGTTTCAAGGCACTAACGTTCTAGACCATTCCATGAGTCATCCAATGCATTTGCACGGCTATAGCTTCTACGTGGTCGGATATGGTTTTGGGAATTGTGACAACGAGACTGACCCAAAACGATTTAATTTGTTTGATCCTCCTCAAGTAACTACCTCTGCGATTCCAAAGAATGGATGGTTGGCCATCAGATTTATTGCAAATAATCCTGGTGTATGGTTTTGGCATTGTCATTTTGAAAAGCACTTGAGTTGGGGTATGGACGTTGCCTTTATAGTGAAGAATGGGGGCACTCCGGAGACTAGCATTCGCCATCCCCCAACCTATATGCCTCCCTGCAAAATTTCGTTAAACTCTCGCGTCCAAGATTTTGATGCAGTTATGAGAAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

254

Amino Acids

29.06

Weight (kDa)

5.68

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_2 PF07731 94 - 219 6.7e-39 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 542
AccII CGCG 1 cut(s) 729
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 1 cut(s) 231
AclI AACGTT 1 cut(s) 387
AclWI GGATC 2 cut(s) 293, 500
AcoI YGGCCR 2 cut(s) 34, 552
AcsI RAATTY 2 cut(s) 166, 711
AcuI CTGAAG 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 542
AgsI TTSAA 5 cut(s) 122, 243, 346, 377, 608
AjnI CCWGG 1 cut(s) 577
AjuI GAANNNNNNNTTGG 4 cut(s) 139, 171, 533, 565
AluBI AGCT 1 cut(s) 435
AluI AGCT 1 cut(s) 435
Alw26I GTCTC 2 cut(s) 470, 662
AlwI GGATC 2 cut(s) 293, 500
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 2 cut(s) 34, 552
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 2 cut(s) 166, 711
AsuC2I CCSGG 1 cut(s) 219
AsuII TTCGAA 1 cut(s) 15
BaeGI GKGCMC 1 cut(s) 662
BalI TGGCCA 2 cut(s) 36, 554
BbvI GCAGC 1 cut(s) 37
BccI CCATC 3 cut(s) 540, 563, 690
BceAI ACGGC 1 cut(s) 442
BciT130I CCWGG 1 cut(s) 579
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 470, 662
BfaI CTAG 2 cut(s) 392, 672
BfmI CTRYAG 1 cut(s) 430
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 219, 579
BmrFI CCNGG 2 cut(s) 219, 579
BmsI GCATC 2 cut(s) 64, 733
Bpu14I TTCGAA 1 cut(s) 15
BpuEI CTTGAG 2 cut(s) 498, 637
BpuMI CCSGG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 442
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 1 cut(s) 542
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 1 cut(s) 579
BseGI GGATG 4 cut(s) 10, 409, 551, 682
BseLI CCNNNNNNNGG 1 cut(s) 542
BseRI GAGGAG 1 cut(s) 501
BseSI GKGCMC 1 cut(s) 662
BseXI GCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 729
BshFI GGCC 2 cut(s) 36, 554
BsiSI CCGG 2 cut(s) 219, 665
BslI CCNNNNNNNGG 1 cut(s) 542
BsmAI GTCTC 2 cut(s) 470, 662
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 2 cut(s) 36, 554
Bsp119I TTCGAA 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 662
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 3 cut(s) 114, 298, 505
BspACI CCGC 1 cut(s) 231
BspANI GGCC 2 cut(s) 36, 554
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 1 cut(s) 729
BspPI GGATC 2 cut(s) 293, 500
BspT104I TTCGAA 1 cut(s) 15
BssMI GATC 3 cut(s) 114, 298, 505
Bst2UI CCWGG 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 442
BstBI TTCGAA 1 cut(s) 15
BstF5I GGATG 4 cut(s) 10, 409, 551, 682
BstFNI CGCG 1 cut(s) 729
BstKTI GATC 3 cut(s) 117, 301, 508
BstMAI GTCTC 2 cut(s) 470, 662
BstMBI GATC 3 cut(s) 114, 298, 505
BstNI CCWGG 1 cut(s) 579
BstSCI CCNGG 2 cut(s) 217, 577
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 662
BstUI CGCG 1 cut(s) 729
BstV1I GCAGC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 36, 554
BtsCI GGATG 4 cut(s) 10, 409, 551, 682
CseI GACGC 1 cut(s) 718
CviAII CATG 3 cut(s) 46, 98, 403
CviJI RGCY 7 cut(s) 28, 36, 50, 193, 429, 435, 554
CviKI_1 RGCY 7 cut(s) 28, 36, 50, 193, 429, 435, 554
DpnI GATC 3 cut(s) 116, 300, 507
DpnII GATC 3 cut(s) 114, 298, 505
EaeI YGGCCR 2 cut(s) 34, 552
Eco57I CTGAAG 1 cut(s) 99
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 577
EcoT22I ATGCAT 1 cut(s) 420
FaeI CATG 3 cut(s) 49, 101, 406
FatI CATG 3 cut(s) 45, 97, 402
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 4 cut(s) 17, 396, 558, 669
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 2 cut(s) 392, 672
GluI GCNGC 1 cut(s) 51
HaeIII GGCC 2 cut(s) 36, 554
HapII CCGG 2 cut(s) 219, 665
HgaI GACGC 1 cut(s) 718
Hin1II CATG 3 cut(s) 49, 101, 406
HinfI GANTC 3 cut(s) 356, 406, 532
HpaII CCGG 2 cut(s) 219, 665
Hpy166II GTNNAC 1 cut(s) 315
Hpy188I TCNGA 5 cut(s) 57, 165, 172, 449, 560
Hpy188III TCNNGA 4 cut(s) 302, 343, 392, 665
Hpy8I GTNNAC 1 cut(s) 315
HpyAV CCTTC 1 cut(s) 165
HpyCH4IV ACGT 3 cut(s) 387, 441, 633
HpyCH4V TGCA 5 cut(s) 418, 424, 569, 708, 746
HpySE526I ACGT 3 cut(s) 387, 441, 633
Hsp92II CATG 3 cut(s) 49, 101, 406
Kpn2I TCCGGA 1 cut(s) 664
Kzo9I GATC 3 cut(s) 114, 298, 505
LpnPI CCDG 8 cut(s) 154, 232, 267, 315, 564, 591, 678, 718
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 2 cut(s) 64, 733
MaeI CTAG 2 cut(s) 392, 672
MaeII ACGT 3 cut(s) 387, 441, 633
MaeIII GTNAC 3 cut(s) 289, 467, 517
MalI GATC 3 cut(s) 116, 300, 507
MboI GATC 3 cut(s) 114, 298, 505
MboII GAAGA 2 cut(s) 261, 661
MhlI GDGCHC 1 cut(s) 662
MlsI TGGCCA 2 cut(s) 36, 554
MluCI AATT 6 cut(s) 88, 166, 346, 463, 496, 711
MluNI TGGCCA 2 cut(s) 36, 554
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 2 cut(s) 170, 427
MnlI CCTC 5 cut(s) 39, 519, 522, 535, 711
Mox20I TGGCCA 2 cut(s) 36, 554
Mph1103I ATGCAT 1 cut(s) 420
MroI TCCGGA 1 cut(s) 664
MscI TGGCCA 2 cut(s) 36, 554
MseI TTAA 2 cut(s) 495, 719
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 2 cut(s) 36, 554
MspI CCGG 2 cut(s) 219, 665
MspR9I CCNGG 2 cut(s) 219, 579
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 1 cut(s) 729
NciI CCSGG 1 cut(s) 219
NdeII GATC 3 cut(s) 114, 298, 505
NlaIII CATG 3 cut(s) 49, 101, 406
NmuCI GTSAC 1 cut(s) 467
NsiI ATGCAT 1 cut(s) 420
NspV TTCGAA 1 cut(s) 15
PctI GAATGC 1 cut(s) 675
PfeI GAWTC 2 cut(s) 356, 532
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Ppu21I YACGTR 1 cut(s) 442
Psp1406I AACGTT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 2 cut(s) 495, 719
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 3 cut(s) 114, 298, 505
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 2 cut(s) 219, 579
SduI GDGCHC 1 cut(s) 662
SetI ASST 9 cut(s) 143, 336, 342, 390, 437, 444, 527, 636, 695
SfaNI GCATC 2 cut(s) 64, 733
SfcI CTRYAG 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 15
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 513, 616
SmoI CTYRAG 2 cut(s) 513, 616
Sse9I AATT 6 cut(s) 88, 166, 346, 463, 496, 711
SsiI CCGC 1 cut(s) 231
SspI AATATT 1 cut(s) 41
SspMI CTAG 2 cut(s) 392, 672
StyD4I CCNGG 2 cut(s) 217, 577
TaiI ACGT 3 cut(s) 390, 444, 636
TaqI TCGA 1 cut(s) 15
TasI AATT 6 cut(s) 88, 166, 346, 463, 496, 711
TfiI GAWTC 2 cut(s) 356, 532
Tru1I TTAA 2 cut(s) 495, 719
Tru9I TTAA 2 cut(s) 495, 719
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 467
Van91I CCANNNNNTGG 1 cut(s) 542
XapI RAATTY 2 cut(s) 166, 711
XbaI TCTAGA 1 cut(s) 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 543
XspI CTAG 2 cut(s) 392, 672
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.