Rh4AG365400

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005702
Physical Location & Seq
Reverse (-)
67337218 .. 67340007
2790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG365400.1

Sequence Viewer

Length: 1131 bp
ATGGGGAGATGGAGAGCAGCTTCTCTTCTCCTCAGGGACATAGCCATAACCACCACACCACCCAAATCTTCTTCTCTTCTTTTCAAGCCTCTCACTCTCTCACGCTCTTCTTCTTCGTCGTCAATCCCCACTTCCCAATTCTACTTCTTCCTCAAGTCTAGGCCTCTCTCTGCAATCCCATCTCGGGTATCAATTGACCCGAATGATTTCGAATCCGGGCCGGCCTATTGCGCTCATGAACAACCCAATGAAGACGACGAAACCGGGAAAATCCCCATCAAGGCGTATTTCCTTTGCACCAGTATCAATTTGAAGAGCATGCAGGCCGAGTATTTAAGCAATGTCATTCCTCCTTCGTCTCGCTCAACCAATTATATTGCACTCAGATTTTGTGATTTCCCTTCAGAAAATAGTAGATTTGGAGTATGGGAGAATCCAAGCTCCTGGCGTTACATGGTAGTATTCCAGTATGGATCAACTGTTCTGTTTAATGTTGAGGAGCATGAAGTTCAGGCTTACCTAAATCTAGTCATAAGACATGCTTCTGGAATGCTACGAGAGATGGTAAAAGACGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTTGACGATATGCAGGGTGGGCCAGACTACATAGTTCTTAGAACTTTGGACACGGATAGTATCCGCATTATTGGAAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGAGGAATGGAAAAAACTGGAACTTTTACTATGCATAAAAAGAAGCTCCTTCAACTCGTTGGAAAGGCTAACTCAACTCTAGCTGACGTAATTGTCAAAGTTGGTCTATTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACCTGAGGGAGGAGTATGAGGTTACGCAACGATTTGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAGTCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTGTAGAGAATATTATATCACTATACGAGATTGTTCGGGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

376

Amino Acids

42.81

Weight (kDa)

5.75

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 152 - 322 7.2e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 712
AciI CCGC 1 cut(s) 667
AclWI GGATC 1 cut(s) 481
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 1 cut(s) 980
AcuI CTGAAG 1 cut(s) 387
AfiI CCNNNNNNNGG 3 cut(s) 184, 280, 931
AgsI TTSAA 5 cut(s) 85, 313, 740, 812, 973
AjnI CCWGG 1 cut(s) 443
AloI GAACNNNNNNTCC 2 cut(s) 465, 497
AluBI AGCT 5 cut(s) 20, 441, 601, 805, 842
AluI AGCT 5 cut(s) 20, 441, 601, 805, 842
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 481
AlwNI CAGNNNCTG 1 cut(s) 1124
Ama87I CYCGRG 1 cut(s) 183
AoxI GGCC 6 cut(s) 161, 218, 222, 324, 623, 688
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 980
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 218, 623
AsuC2I CCSGG 2 cut(s) 217, 265
AsuII TTCGAA 1 cut(s) 210
AvaI CYCGRG 1 cut(s) 183
AxyI CCTNAGG 2 cut(s) 32, 926
BalI TGGCCA 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 993
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 1 cut(s) 29
BccI CCATC 5 cut(s) 3, 187, 284, 556, 722
BciT130I CCWGG 1 cut(s) 445
BciVI GTATCC 1 cut(s) 674
BcnI CCSGG 2 cut(s) 217, 265
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 3 cut(s) 159, 527, 839
BfmI CTRYAG 1 cut(s) 1071
BfuAI ACCTGC 1 cut(s) 712
BfuI GTATCC 1 cut(s) 674
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 217, 265, 445
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 218, 623
BmrFI CCNGG 3 cut(s) 217, 265, 445
BmsI GCATC 2 cut(s) 886, 1062
BpiI GAAGAC 1 cut(s) 258
Bpu10I CCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 2 cut(s) 217, 265
BsaBI GATNNNNATC 1 cut(s) 756
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 280, 931
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 3 cut(s) 300, 466, 781
Bse21I CCTNAGG 2 cut(s) 32, 926
Bse3DI GCAATG 1 cut(s) 346
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 901
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 3 cut(s) 184, 280, 931
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 4 cut(s) 46, 397, 611, 917
BseNI ACTGG 3 cut(s) 300, 466, 781
BseRI GAGGAG 3 cut(s) 20, 512, 947
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 6 cut(s) 163, 220, 224, 326, 625, 690
BsiHKAI GWGCWC 1 cut(s) 993
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 3 cut(s) 216, 221, 264
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 3 cut(s) 184, 280, 931
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 6 cut(s) 163, 220, 224, 326, 625, 690
BsoBI CYCGRG 1 cut(s) 183
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 3 cut(s) 473, 875, 1036
BspACI CCGC 1 cut(s) 667
BspANI GGCC 6 cut(s) 163, 220, 224, 326, 625, 690
BspCNI CTCAG 4 cut(s) 45, 396, 610, 918
BspHI TCATGA 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 1 cut(s) 481
BspQI GCTCTTC 2 cut(s) 112, 308
BspT104I TTCGAA 1 cut(s) 210
BsrDI GCAATG 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 3 cut(s) 300, 466, 781
BssAI RCCGGY 1 cut(s) 220
BssMI GATC 3 cut(s) 473, 875, 1036
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 481, 1072
Bst6I CTCTTC 5 cut(s) 30, 81, 112, 308, 735
BstAPI GCANNNNNTGC 1 cut(s) 905
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 3 cut(s) 222, 320, 324
BstDEI CTNAG 5 cut(s) 32, 383, 597, 641, 926
BstENI CCTNNNNNAGG 1 cut(s) 929
BstF5I GGATG 1 cut(s) 901
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 3 cut(s) 476, 878, 1039
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 3 cut(s) 473, 875, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 230, 622, 905
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 2 cut(s) 322, 542
BstSCI CCNGG 3 cut(s) 215, 263, 443
BstSFI CTRYAG 1 cut(s) 1071
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 258
BstXI CCANNNNNNTGG 1 cut(s) 444
Bsu36I CCTNAGG 2 cut(s) 32, 926
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 6 cut(s) 163, 220, 224, 326, 625, 690
BtsCI GGATG 1 cut(s) 901
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 3 cut(s) 222, 320, 324
CaiI CAGNNNCTG 1 cut(s) 1124
CciI TCATGA 1 cut(s) 235
CfoI GCGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 2 cut(s) 218, 623
CviAII CATG 6 cut(s) 236, 319, 454, 503, 539, 917
DdeI CTNAG 5 cut(s) 32, 383, 597, 641, 926
DpnI GATC 3 cut(s) 475, 877, 1038
DpnII GATC 3 cut(s) 473, 875, 1036
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 688
Eam1104I CTCTTC 5 cut(s) 30, 81, 112, 308, 735
EarI CTCTTC 5 cut(s) 30, 81, 112, 308, 735
Eco147I AGGCCT 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 387
Eco81I CCTNAGG 2 cut(s) 32, 926
Eco88I CYCGRG 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 443
EcoT22I ATGCAT 1 cut(s) 795
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 6 cut(s) 239, 322, 457, 506, 542, 920
FalI AAGNNNNNCTT 4 cut(s) 526, 558, 685, 717
FaqI GGGAC 1 cut(s) 50
FatI CATG 6 cut(s) 235, 318, 453, 502, 538, 916
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 908
FseI GGCCGGCC 1 cut(s) 224
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 3 cut(s) 159, 527, 839
GlaI GCGC 1 cut(s) 232
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 6 cut(s) 163, 220, 224, 326, 625, 690
HapII CCGG 3 cut(s) 216, 221, 264
HhaI GCGC 1 cut(s) 233
Hin1II CATG 6 cut(s) 239, 322, 457, 506, 542, 920
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 2 cut(s) 607, 1068
HindII GTYRAC 2 cut(s) 607, 1068
HinfI GANTC 3 cut(s) 212, 433, 1109
HpaII CCGG 3 cut(s) 216, 221, 264
Hpy166II GTNNAC 2 cut(s) 607, 1068
Hpy188I TCNGA 4 cut(s) 386, 406, 880, 1036
Hpy188III TCNNGA 4 cut(s) 236, 546, 1043, 1106
Hpy8I GTNNAC 2 cut(s) 607, 1068
Hpy99I CGWCG 2 cut(s) 121, 260
HpyAV CCTTC 3 cut(s) 363, 411, 818
HpyCH4III ACNGT 2 cut(s) 481, 1072
HpyCH4IV ACGT 1 cut(s) 846
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 622, 905
HpyF3I CTNAG 5 cut(s) 32, 383, 597, 641, 926
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 6 cut(s) 239, 322, 457, 506, 542, 920
HspAI GCGC 1 cut(s) 231
KroI GCCGGC 1 cut(s) 220
KroNI GCCGGC 1 cut(s) 222
Kzo9I GATC 3 cut(s) 473, 875, 1036
LguI GCTCTTC 2 cut(s) 112, 308
LmnI GCTCC 3 cut(s) 446, 499, 810
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 886, 1062
MaeI CTAG 3 cut(s) 159, 527, 839
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 2 cut(s) 449, 943
MalI GATC 3 cut(s) 475, 877, 1038
MboI GATC 3 cut(s) 473, 875, 1036
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 993
MlsI TGGCCA 1 cut(s) 690
MluCI AATT 7 cut(s) 137, 192, 307, 370, 849, 882, 980
MluNI TGGCCA 1 cut(s) 690
MmeI TCCRAC 1 cut(s) 799
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 795
MroNI GCCGGC 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 690
MseI TTAA 3 cut(s) 335, 489, 977
Msp20I TGGCCA 1 cut(s) 690
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 3 cut(s) 216, 221, 264
MspR9I CCNGG 3 cut(s) 217, 265, 445
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 3 cut(s) 230, 622, 905
NaeI GCCGGC 1 cut(s) 222
NciI CCSGG 2 cut(s) 217, 265
NdeII GATC 3 cut(s) 473, 875, 1036
NgoMIV GCCGGC 1 cut(s) 220
NlaIII CATG 6 cut(s) 239, 322, 457, 506, 542, 920
NmeAIII GCCGAG 1 cut(s) 352
NsiI ATGCAT 1 cut(s) 795
NspI RCATGY 2 cut(s) 322, 542
NspV TTCGAA 1 cut(s) 210
PaeI GCATGC 1 cut(s) 322
PagI TCATGA 1 cut(s) 235
PceI AGGCCT 1 cut(s) 163
PciSI GCTCTTC 2 cut(s) 112, 308
PctI GAATGC 1 cut(s) 555
PdiI GCCGGC 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 3 cut(s) 212, 433, 1109
PkrI GCNGC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspPI GGNCC 2 cut(s) 218, 623
PstNI CAGNNNCTG 1 cut(s) 1124
PvuII CAGCTG 1 cut(s) 601
RigI GGCCGGCC 1 cut(s) 224
SapI GCTCTTC 2 cut(s) 112, 308
SaqAI TTAA 3 cut(s) 335, 489, 977
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 473, 875, 1036
Sau96I GGNCC 2 cut(s) 218, 623
ScrFI CCNGG 3 cut(s) 217, 265, 445
SduI GDGCHC 1 cut(s) 993
SfaNI GCATC 2 cut(s) 886, 1062
SfcI CTRYAG 1 cut(s) 1071
SfuI TTCGAA 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SphI GCATGC 1 cut(s) 322
Sse9I AATT 7 cut(s) 137, 192, 307, 370, 849, 882, 980
SseBI AGGCCT 1 cut(s) 163
SsiI CCGC 1 cut(s) 667
SspI AATATT 1 cut(s) 1081
SspMI CTAG 3 cut(s) 159, 527, 839
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 3 cut(s) 215, 263, 443
TaaI ACNGT 2 cut(s) 481, 1072
TaiI ACGT 1 cut(s) 849
TaqI TCGA 2 cut(s) 210, 760
TasI AATT 7 cut(s) 137, 192, 307, 370, 849, 882, 980
TfiI GAWTC 3 cut(s) 212, 433, 1109
Tru1I TTAA 3 cut(s) 335, 489, 977
Tru9I TTAA 3 cut(s) 335, 489, 977
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 252, 264, 519, 933, 989
TspGWI ACGGA 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 929
XapI RAATTY 1 cut(s) 980
XceI RCATGY 2 cut(s) 322, 542
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 3 cut(s) 159, 527, 839
Zsp2I ATGCAT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.