Rw0G007370

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0005702
Physical Location & Seq
Reverse (-)
191384 .. 194102
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007370.1

Sequence Viewer

Length: 1125 bp
ATGGGGAGATGGAGAGCAGCTTCTCTTCTCCTCAGGAACATAGCCATAACCACCACACCACCCAAATCTTCTTCTCTTCTTTTCAAGCCTCTCACTCTCTCACGCTCTTCTTCTTCTTCAATCCCCACTTCCCAATTCTACTTCTTCCTCAAGTCTAGGCCTCTCTCTGCAATCCCATCTCGGGTCTCAATTGACCCGACTGATTTCGAATCCGGGCCGGCCTATTGCGCTCATGAACAACCCAATGAAGACGACGAAACCGGGAAAATCCCCATTAAGGCGTATTTCCTTTGCACCAGTATCAATTTGAAGAGCATGCAGGCCGAGTATTTAAGCAATGTCATTCCTCCTTCGTCTCGCTCAACCAATTATATTGCACTCAGATTTTGTGATTTCCCTTCGGAAAATAGTATTTGGAGTATGGGAGAATCCAAGCTCCTGGCGTACATGGTAGTATTCCAGTATGGATCAACTGTTCTGTTTAATGTTGAGGAGCATGAAGTTCAGGCTTACCTAAATCTAGTCATAAGACATGCTTCTGGAATGCTACGAGAGATGGTGAAAGACGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTCGACGATATGCAGGGTGGGCCAGACTACATAGTTCTTAGAACTTTGGACACAGATAGTATCCGCATTATTGGAAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGAGGAATGGAAAAAACTGGAACTTTTACCATGCATAAAAAGAAGCTCCTTCAACTCGTTGGAAAGGCTAACTCAACTCTAGCTGATGTAATTGTCAAAGTTGGTCTATTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACCTGAGGGAGGAGTATGAGGTTACGCAGCGATTTGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAGTCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTGTAGAGAATATTATATCACTATACGAGATTGTTCGTGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

374

Amino Acids

42.42

Weight (kDa)

5.75

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 150 - 320 7.1e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 600
AciI CCGC 1 cut(s) 661
AclWI GGATC 1 cut(s) 475
AcoI YGGCCR 1 cut(s) 682
AcsI RAATTY 1 cut(s) 974
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 3 cut(s) 181, 277, 925
AgsI TTSAA 6 cut(s) 85, 120, 310, 734, 806, 967
AjnI CCWGG 1 cut(s) 438
AloI GAACNNNNNNTCC 2 cut(s) 459, 491
AluBI AGCT 5 cut(s) 20, 436, 595, 799, 836
AluI AGCT 5 cut(s) 20, 436, 595, 799, 836
Alw21I GWGCWC 1 cut(s) 987
Alw26I GTCTC 2 cut(s) 190, 360
AlwI GGATC 1 cut(s) 475
AlwNI CAGNNNCTG 1 cut(s) 1118
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 6 cut(s) 158, 215, 219, 321, 617, 682
ApeKI GCWGC 2 cut(s) 17, 943
ApoI RAATTY 1 cut(s) 974
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 215, 617
AsuC2I CCSGG 2 cut(s) 214, 262
AsuHPI GGTGA 1 cut(s) 571
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 180
AxyI CCTNAGG 2 cut(s) 32, 920
BalI TGGCCA 1 cut(s) 684
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 987
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 2 cut(s) 29, 955
BccI CCATC 4 cut(s) 3, 184, 550, 716
BciT130I CCWGG 1 cut(s) 440
BciVI GTATCC 1 cut(s) 668
BcnI CCSGG 2 cut(s) 214, 262
BcoDI GTCTC 2 cut(s) 190, 360
BfaI CTAG 3 cut(s) 156, 521, 833
BfmI CTRYAG 1 cut(s) 1065
BfuAI ACCTGC 1 cut(s) 706
BfuI GTATCC 1 cut(s) 668
BisI GCNGC 2 cut(s) 18, 944
BlsI GCNGC 2 cut(s) 19, 945
Bme1390I CCNGG 3 cut(s) 214, 262, 440
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 215, 617
BmrFI CCNGG 3 cut(s) 214, 262, 440
BmsI GCATC 2 cut(s) 880, 1056
BpiI GAAGAC 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 591
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 214, 262
BsaBI GATNNNNATC 1 cut(s) 750
BsaI GGTCTC 1 cut(s) 190
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 277, 925
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 3 cut(s) 297, 460, 775
Bse21I CCTNAGG 2 cut(s) 32, 920
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 1 cut(s) 440
BseGI GGATG 1 cut(s) 895
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 3 cut(s) 181, 277, 925
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 4 cut(s) 46, 394, 605, 911
BseNI ACTGG 3 cut(s) 297, 460, 775
BseRI GAGGAG 3 cut(s) 20, 506, 941
BseXI GCAGC 2 cut(s) 29, 955
BshFI GGCC 6 cut(s) 160, 217, 221, 323, 619, 684
BsiHKAI GWGCWC 1 cut(s) 987
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 213, 218, 261
BslI CCNNNNNNNGG 3 cut(s) 181, 277, 925
BsmAI GTCTC 2 cut(s) 190, 360
BsmBI CGTCTC 1 cut(s) 360
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 6 cut(s) 160, 217, 221, 323, 619, 684
Bso31I GGTCTC 1 cut(s) 190
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 987
Bsp143I GATC 3 cut(s) 467, 869, 1030
BspACI CCGC 1 cut(s) 661
BspANI GGCC 6 cut(s) 160, 217, 221, 323, 619, 684
BspCNI CTCAG 4 cut(s) 45, 393, 604, 912
BspHI TCATGA 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 1 cut(s) 475
BspQI GCTCTTC 2 cut(s) 112, 305
BspT104I TTCGAA 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 3 cut(s) 297, 460, 775
BssAI RCCGGY 1 cut(s) 217
BssMI GATC 3 cut(s) 467, 869, 1030
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 2 cut(s) 475, 1066
Bst6I CTCTTC 5 cut(s) 30, 81, 112, 305, 729
BstAPI GCANNNNNTGC 1 cut(s) 899
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 219, 317, 321
BstDEI CTNAG 5 cut(s) 32, 380, 591, 635, 920
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 1 cut(s) 895
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 3 cut(s) 470, 872, 1033
BstMAI GTCTC 2 cut(s) 190, 360
BstMBI GATC 3 cut(s) 467, 869, 1030
BstMWI GCNNNNNNNGC 3 cut(s) 227, 616, 899
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 2 cut(s) 319, 536
BstSCI CCNGG 3 cut(s) 212, 260, 438
BstSFI CTRYAG 1 cut(s) 1065
BstV1I GCAGC 2 cut(s) 29, 955
BstV2I GAAGAC 1 cut(s) 255
BstXI CCANNNNNNTGG 1 cut(s) 439
Bsu36I CCTNAGG 2 cut(s) 32, 920
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 6 cut(s) 160, 217, 221, 323, 619, 684
BtsCI GGATG 1 cut(s) 895
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 3 cut(s) 219, 317, 321
CaiI CAGNNNCTG 1 cut(s) 1118
CciI TCATGA 1 cut(s) 232
CfoI GCGC 1 cut(s) 230
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 215, 617
Csp6I GTAC 1 cut(s) 445
CviAII CATG 7 cut(s) 233, 316, 448, 497, 533, 784, 911
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 5 cut(s) 32, 380, 591, 635, 920
DpnI GATC 3 cut(s) 469, 871, 1032
DpnII GATC 3 cut(s) 467, 869, 1030
EaeI YGGCCR 1 cut(s) 682
Eam1104I CTCTTC 5 cut(s) 30, 81, 112, 305, 729
EarI CTCTTC 5 cut(s) 30, 81, 112, 305, 729
Eco147I AGGCCT 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 190
Eco81I CCTNAGG 2 cut(s) 32, 920
Eco88I CYCGRG 1 cut(s) 180
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 789
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 7 cut(s) 236, 319, 451, 500, 536, 787, 914
FalI AAGNNNNNCTT 4 cut(s) 520, 552, 679, 711
FatI CATG 7 cut(s) 232, 315, 447, 496, 532, 783, 910
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 2 cut(s) 18, 944
FokI GGATG 1 cut(s) 902
FseI GGCCGGCC 1 cut(s) 221
Fsp4HI GCNGC 2 cut(s) 18, 944
FspBI CTAG 3 cut(s) 156, 521, 833
GlaI GCGC 1 cut(s) 229
GluI GCNGC 2 cut(s) 18, 944
HaeIII GGCC 6 cut(s) 160, 217, 221, 323, 619, 684
HapII CCGG 3 cut(s) 213, 218, 261
HhaI GCGC 1 cut(s) 230
Hin1II CATG 7 cut(s) 236, 319, 451, 500, 536, 787, 914
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 2 cut(s) 601, 1062
HindII GTYRAC 2 cut(s) 601, 1062
HinfI GANTC 3 cut(s) 209, 428, 1103
HpaII CCGG 3 cut(s) 213, 218, 261
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 2 cut(s) 601, 1062
Hpy188I TCNGA 4 cut(s) 383, 403, 874, 1030
Hpy188III TCNNGA 5 cut(s) 34, 233, 540, 1037, 1100
Hpy8I GTNNAC 2 cut(s) 601, 1062
Hpy99I CGWCG 2 cut(s) 257, 605
HpyAV CCTTC 3 cut(s) 360, 408, 812
HpyCH4III ACNGT 2 cut(s) 475, 1066
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 616, 899
HpyF3I CTNAG 5 cut(s) 32, 380, 591, 635, 920
Hsp92II CATG 7 cut(s) 236, 319, 451, 500, 536, 787, 914
HspAI GCGC 1 cut(s) 228
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
Kzo9I GATC 3 cut(s) 467, 869, 1030
LguI GCTCTTC 2 cut(s) 112, 305
LmnI GCTCC 3 cut(s) 441, 493, 804
Lsp1109I GCAGC 2 cut(s) 29, 955
LweI GCATC 2 cut(s) 880, 1056
MaeI CTAG 3 cut(s) 156, 521, 833
MaeIII GTNAC 1 cut(s) 937
MalI GATC 3 cut(s) 469, 871, 1032
MboI GATC 3 cut(s) 467, 869, 1030
MfeI CAATTG 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 987
MlsI TGGCCA 1 cut(s) 684
MluCI AATT 7 cut(s) 134, 189, 304, 367, 843, 876, 974
MluNI TGGCCA 1 cut(s) 684
MmeI TCCRAC 1 cut(s) 793
Mox20I TGGCCA 1 cut(s) 684
Mph1103I ATGCAT 1 cut(s) 789
MroNI GCCGGC 1 cut(s) 217
MscI TGGCCA 1 cut(s) 684
MseI TTAA 4 cut(s) 276, 332, 483, 971
Msp20I TGGCCA 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 3 cut(s) 213, 218, 261
MspR9I CCNGG 3 cut(s) 214, 262, 440
MunI CAATTG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 549
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 227, 616, 899
NaeI GCCGGC 1 cut(s) 219
NciI CCSGG 2 cut(s) 214, 262
NdeII GATC 3 cut(s) 467, 869, 1030
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 7 cut(s) 236, 319, 451, 500, 536, 787, 914
NmeAIII GCCGAG 1 cut(s) 349
NsiI ATGCAT 1 cut(s) 789
NspI RCATGY 2 cut(s) 319, 536
NspV TTCGAA 1 cut(s) 207
PaeI GCATGC 1 cut(s) 319
PagI TCATGA 1 cut(s) 232
PceI AGGCCT 1 cut(s) 160
PciSI GCTCTTC 2 cut(s) 112, 305
PctI GAATGC 1 cut(s) 549
PdiI GCCGGC 1 cut(s) 219
PfeI GAWTC 3 cut(s) 209, 428, 1103
PkrI GCNGC 2 cut(s) 19, 945
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspPI GGNCC 2 cut(s) 215, 617
PstNI CAGNNNCTG 1 cut(s) 1118
PvuII CAGCTG 1 cut(s) 595
RigI GGCCGGCC 1 cut(s) 221
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SalI GTCGAC 1 cut(s) 599
SapI GCTCTTC 2 cut(s) 112, 305
SaqAI TTAA 4 cut(s) 276, 332, 483, 971
SatI GCNGC 2 cut(s) 18, 944
Sau3AI GATC 3 cut(s) 467, 869, 1030
Sau96I GGNCC 2 cut(s) 215, 617
ScrFI CCNGG 3 cut(s) 214, 262, 440
SduI GDGCHC 1 cut(s) 987
SfaNI GCATC 2 cut(s) 880, 1056
SfcI CTRYAG 1 cut(s) 1065
SfuI TTCGAA 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SphI GCATGC 1 cut(s) 319
Sse9I AATT 7 cut(s) 134, 189, 304, 367, 843, 876, 974
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 1 cut(s) 661
SspI AATATT 1 cut(s) 1075
SspMI CTAG 3 cut(s) 156, 521, 833
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 3 cut(s) 212, 260, 438
TaaI ACNGT 2 cut(s) 475, 1066
TaqI TCGA 3 cut(s) 207, 600, 754
TasI AATT 7 cut(s) 134, 189, 304, 367, 843, 876, 974
TfiI GAWTC 3 cut(s) 209, 428, 1103
Tru1I TTAA 4 cut(s) 276, 332, 483, 971
Tru9I TTAA 4 cut(s) 276, 332, 483, 971
TseI GCWGC 2 cut(s) 17, 943
TspDTI ATGAA 5 cut(s) 249, 261, 513, 927, 983
XagI CCTNNNNNAGG 1 cut(s) 923
XapI RAATTY 1 cut(s) 974
XceI RCATGY 2 cut(s) 319, 536
XmiI GTMKAC 1 cut(s) 600
XspI CTAG 3 cut(s) 156, 521, 833
Zsp2I ATGCAT 1 cut(s) 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.