Rroxscaffold_5G00379980

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005702
Physical Location & Seq
Reverse (-)
60166547 .. 60169334
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379980.1

Sequence Viewer

Length: 1110 bp
ATGGGGAGATGGAGAGCAGCTTCTCTTCTCCTCAAGAACATAGCCATAACCACCACACCACCCAAATCTTCTTCTCTTGCTTTCAAGCCTCCTTCTTCTTCAACCCCCACTTCCCAATTCTACTTCTTCCTCAAGTCGAGGCCTTTCTCTGCAATCCCATCTCGGGTCTCAATTGACCCGACTGATTTCGAATCCGGGCCGGCCTATTGCGCTCATGAACAACCCAATGAAGACGACGAAACCGGGAAAATCCCCATTAAGGCGTATTTCCTTTGCACCAGTATCAATTTGAAGAGCATGCAGGCCGAGTATTTAAGCAATGTCATTCCTCCTTCGTCTCGCTCGACCAATCATATTGCACTCAGATTTTGTGATTTTCCTTCGGATAATAGTAGATTTGGAGTATGGGAGAATCCAAGCTCCTGGCGTTACATGGTAGTATTCCAGTATGGATCAACTGTTCTGTTTAATGTTGAGGAGCATGAAGTTCAGGCTTACCTAAATCTAGTCATAAGACATGCTTCTGGAATGCTACGAGAGATGGTGAAAGACGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTTGACGACATGCAGGGTGGGCCAGACTACATAGTTCTTAGAACTTTGGACACGGATAGTATCCGCATTATTGGAAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGAGGAATGGAAAAAACTGGAACTTTTACTATGCATAAAAAGAAGCTCCTTCAACTCGTTGGAAAGGCTAACTCAACTCTAGCTGACATAATTGTCAAAGTTGGTCTATTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACCTGAGGGAGGAGTATGAGGTTACACAACGATTTGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAGTCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTGTAGAGAATATTATATCACTATACGAGATTGTTCGTGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

369

Amino Acids

41.99

Weight (kDa)

5.83

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 145 - 315 4.6e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 830
Acc36I ACCTGC 1 cut(s) 691
AciI CCGC 1 cut(s) 646
AclWI GGATC 1 cut(s) 460
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 1 cut(s) 959
AfiI CCNNNNNNNGG 3 cut(s) 163, 259, 910
AgsI TTSAA 6 cut(s) 85, 102, 292, 719, 791, 952
AjnI CCWGG 1 cut(s) 422
AloI GAACNNNNNNTCC 2 cut(s) 444, 476
AluBI AGCT 5 cut(s) 20, 420, 580, 784, 821
AluI AGCT 5 cut(s) 20, 420, 580, 784, 821
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 2 cut(s) 172, 342
AlwI GGATC 1 cut(s) 460
AlwNI CAGNNNCTG 1 cut(s) 1103
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 6 cut(s) 140, 197, 201, 303, 602, 667
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 959
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 197, 602
AsuC2I CCSGG 2 cut(s) 196, 244
AsuHPI GGTGA 1 cut(s) 556
AsuII TTCGAA 1 cut(s) 189
AvaI CYCGRG 1 cut(s) 162
AxyI CCTNAGG 1 cut(s) 905
BalI TGGCCA 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 972
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 29
BccI CCATC 4 cut(s) 3, 166, 535, 701
BciT130I CCWGG 1 cut(s) 424
BciVI GTATCC 1 cut(s) 653
BcnI CCSGG 2 cut(s) 196, 244
BcoDI GTCTC 2 cut(s) 172, 342
BfaI CTAG 2 cut(s) 506, 818
BfmI CTRYAG 1 cut(s) 1050
BfuAI ACCTGC 1 cut(s) 691
BfuI GTATCC 1 cut(s) 653
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 196, 244, 424
BmeT110I CYCGRG 1 cut(s) 162
BmgT120I GGNCC 2 cut(s) 197, 602
BmrFI CCNGG 3 cut(s) 196, 244, 424
BmsI GCATC 2 cut(s) 865, 1041
BpiI GAAGAC 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 576
Bpu14I TTCGAA 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 17, 116
BpuMI CCSGG 2 cut(s) 196, 244
BsaBI GATNNNNATC 1 cut(s) 735
BsaI GGTCTC 1 cut(s) 172
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 259, 910
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 3 cut(s) 279, 445, 760
Bse21I CCTNAGG 1 cut(s) 905
Bse3DI GCAATG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 1 cut(s) 424
BseGI GGATG 1 cut(s) 880
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 3 cut(s) 163, 259, 910
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 376, 590, 896
BseNI ACTGG 3 cut(s) 279, 445, 760
BseRI GAGGAG 3 cut(s) 20, 491, 926
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 6 cut(s) 142, 199, 203, 305, 604, 669
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 3 cut(s) 195, 200, 243
BslI CCNNNNNNNGG 3 cut(s) 163, 259, 910
BsmAI GTCTC 2 cut(s) 172, 342
BsmBI CGTCTC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 534
BsnI GGCC 6 cut(s) 142, 199, 203, 305, 604, 669
Bso31I GGTCTC 1 cut(s) 172
BsoBI CYCGRG 1 cut(s) 162
Bsp119I TTCGAA 1 cut(s) 189
Bsp1286I GDGCHC 1 cut(s) 972
Bsp143I GATC 3 cut(s) 452, 854, 1015
BspACI CCGC 1 cut(s) 646
BspANI GGCC 6 cut(s) 142, 199, 203, 305, 604, 669
BspCNI CTCAG 3 cut(s) 375, 589, 897
BspHI TCATGA 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 1 cut(s) 460
BspQI GCTCTTC 1 cut(s) 287
BspT104I TTCGAA 1 cut(s) 189
BspTNI GGTCTC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 3 cut(s) 279, 445, 760
BssAI RCCGGY 1 cut(s) 199
BssMI GATC 3 cut(s) 452, 854, 1015
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 2 cut(s) 460, 1051
Bst6I CTCTTC 3 cut(s) 30, 287, 714
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 189
BstC8I GCNNGC 3 cut(s) 201, 299, 303
BstDEI CTNAG 4 cut(s) 362, 576, 620, 905
BstENI CCTNNNNNAGG 1 cut(s) 908
BstF5I GGATG 1 cut(s) 880
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 455, 857, 1018
BstMAI GTCTC 2 cut(s) 172, 342
BstMBI GATC 3 cut(s) 452, 854, 1015
BstMWI GCNNNNNNNGC 3 cut(s) 209, 601, 884
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 3 cut(s) 301, 521, 595
BstSCI CCNGG 3 cut(s) 194, 242, 422
BstSFI CTRYAG 1 cut(s) 1050
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 423
Bsu36I CCTNAGG 1 cut(s) 905
BsuI GTATCC 1 cut(s) 653
BsuRI GGCC 6 cut(s) 142, 199, 203, 305, 604, 669
BtsCI GGATG 1 cut(s) 880
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 3 cut(s) 201, 299, 303
CaiI CAGNNNCTG 1 cut(s) 1103
CciI TCATGA 1 cut(s) 214
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 197, 602
CviAII CATG 7 cut(s) 215, 298, 433, 482, 518, 592, 896
DdeI CTNAG 4 cut(s) 362, 576, 620, 905
DpnI GATC 3 cut(s) 454, 856, 1017
DpnII GATC 3 cut(s) 452, 854, 1015
DrdI GACNNNNNNGTC 1 cut(s) 830
DseDI GACNNNNNNGTC 1 cut(s) 830
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 3 cut(s) 30, 287, 714
EarI CTCTTC 3 cut(s) 30, 287, 714
Eco147I AGGCCT 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 162
EcoNI CCTNNNNNAGG 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 422
EcoT22I ATGCAT 1 cut(s) 774
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 7 cut(s) 218, 301, 436, 485, 521, 595, 899
FalI AAGNNNNNCTT 4 cut(s) 505, 537, 664, 696
FatI CATG 7 cut(s) 214, 297, 432, 481, 517, 591, 895
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 887
FseI GGCCGGCC 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 2 cut(s) 506, 818
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 6 cut(s) 142, 199, 203, 305, 604, 669
HapII CCGG 3 cut(s) 195, 200, 243
HhaI GCGC 1 cut(s) 212
Hin1II CATG 7 cut(s) 218, 301, 436, 485, 521, 595, 899
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 2 cut(s) 586, 1047
HindII GTYRAC 2 cut(s) 586, 1047
HinfI GANTC 3 cut(s) 191, 412, 1088
HpaII CCGG 3 cut(s) 195, 200, 243
HphI GGTGA 1 cut(s) 556
Hpy166II GTNNAC 2 cut(s) 586, 1047
Hpy188I TCNGA 4 cut(s) 365, 385, 859, 1015
Hpy188III TCNNGA 5 cut(s) 34, 215, 525, 1022, 1085
Hpy8I GTNNAC 2 cut(s) 586, 1047
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 4 cut(s) 102, 342, 390, 797
HpyCH4III ACNGT 2 cut(s) 460, 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 601, 884
HpyF3I CTNAG 4 cut(s) 362, 576, 620, 905
Hsp92II CATG 7 cut(s) 218, 301, 436, 485, 521, 595, 899
HspAI GCGC 1 cut(s) 210
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 3 cut(s) 452, 854, 1015
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 3 cut(s) 425, 478, 789
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 865, 1041
MaeI CTAG 2 cut(s) 506, 818
MaeIII GTNAC 2 cut(s) 428, 922
MalI GATC 3 cut(s) 454, 856, 1017
MboI GATC 3 cut(s) 452, 854, 1015
MfeI CAATTG 1 cut(s) 171
MhlI GDGCHC 1 cut(s) 972
MlsI TGGCCA 1 cut(s) 669
MluCI AATT 6 cut(s) 116, 171, 286, 828, 861, 959
MluNI TGGCCA 1 cut(s) 669
MmeI TCCRAC 1 cut(s) 778
Mox20I TGGCCA 1 cut(s) 669
Mph1103I ATGCAT 1 cut(s) 774
MroNI GCCGGC 1 cut(s) 199
MscI TGGCCA 1 cut(s) 669
MseI TTAA 4 cut(s) 258, 314, 468, 956
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 580
MspI CCGG 3 cut(s) 195, 200, 243
MspR9I CCNGG 3 cut(s) 196, 244, 424
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 534
MvaI CCWGG 1 cut(s) 424
MwoI GCNNNNNNNGC 3 cut(s) 209, 601, 884
NaeI GCCGGC 1 cut(s) 201
NciI CCSGG 2 cut(s) 196, 244
NdeII GATC 3 cut(s) 452, 854, 1015
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 7 cut(s) 218, 301, 436, 485, 521, 595, 899
NmeAIII GCCGAG 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 774
NspI RCATGY 3 cut(s) 301, 521, 595
NspV TTCGAA 1 cut(s) 189
PaeI GCATGC 1 cut(s) 301
PagI TCATGA 1 cut(s) 214
PceI AGGCCT 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 341
PctI GAATGC 1 cut(s) 534
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 191, 412, 1088
PkrI GCNGC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspPI GGNCC 2 cut(s) 197, 602
PstNI CAGNNNCTG 1 cut(s) 1103
PvuII CAGCTG 1 cut(s) 580
RigI GGCCGGCC 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 4 cut(s) 258, 314, 468, 956
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 452, 854, 1015
Sau96I GGNCC 2 cut(s) 197, 602
ScrFI CCNGG 3 cut(s) 196, 244, 424
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 2 cut(s) 865, 1041
SfcI CTRYAG 1 cut(s) 1050
SfuI TTCGAA 1 cut(s) 189
SmlI CTYRAG 2 cut(s) 32, 131
SmoI CTYRAG 2 cut(s) 32, 131
SphI GCATGC 1 cut(s) 301
Sse9I AATT 6 cut(s) 116, 171, 286, 828, 861, 959
SseBI AGGCCT 1 cut(s) 142
SsiI CCGC 1 cut(s) 646
SspI AATATT 1 cut(s) 1060
SspMI CTAG 2 cut(s) 506, 818
StuI AGGCCT 1 cut(s) 142
StyD4I CCNGG 3 cut(s) 194, 242, 422
TaaI ACNGT 2 cut(s) 460, 1051
TaqI TCGA 4 cut(s) 137, 189, 344, 739
TasI AATT 6 cut(s) 116, 171, 286, 828, 861, 959
TfiI GAWTC 3 cut(s) 191, 412, 1088
Tru1I TTAA 4 cut(s) 258, 314, 468, 956
Tru9I TTAA 4 cut(s) 258, 314, 468, 956
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 5 cut(s) 231, 243, 498, 912, 968
TspGWI ACGGA 1 cut(s) 650
XagI CCTNNNNNAGG 1 cut(s) 908
XapI RAATTY 1 cut(s) 959
XceI RCATGY 3 cut(s) 301, 521, 595
XspI CTAG 2 cut(s) 506, 818
Zsp2I ATGCAT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.