Rh4CG390700

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005702
Physical Location & Seq
Reverse (-)
64110851 .. 64113564
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG390700.1

Sequence Viewer

Length: 1140 bp
ATGGGGAGATGGAGAGCAGCTTCTCTTCTCCTCAAGAACATAGCCATAACCACCACACCACCCAAATCTTCTTCTCTTCTTTTCAAGCCTCCCAGTCTCTCACGCTCTTCTTCTTCTTCTTCTTCTTCTTCAATCCCCACTTCCCAATTCTACTTGTTCCTCAAGTCTAGGCCTTTCTCTGCAATCCCATCTCGGGTCTCAATTGACCCGAATGATTTCGATTCCGGGCCGGCCTATTGCGCTCACGAACAACCCAATGAAGACGACGAAACCGGGAAAATCCCCATTAAGGCGTATTTCCTTTGCACCAGTATCAATTTGAAGAGCATGCAGGCCGAGTATTTAAGCAATGTCATTCCTCCTTCGTCTCGCTCAACCAATTATATTGCACTCAGATTTTGTGATTTCCCTTCGGAAAATAGTAGATTTGGAGTATGGGAGAATCCAAGCTCCTGGCGTTACATGGTAGTATTCCAGTATGGATCAACTGTTCTGTTTAATGTTGAGGAGCATGAAGTTCAGGCTTACCTAAATCTAGTCATAAGACATGCTTCTGGAATGCTACGAGAGATGGTCAAAGACGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTTGACGATATGCAGGGTGGGCCAGACTACATAGTTCTTAGAACTTTGGACACGGATAGTATCCGCATTATTGGAAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGAGGAATGGAAAAAACTGGAACTTTTACTATGCATAAAAAGAAGCTCCTTCAACTCGTTGGAAAGGCTAACTCAACTCTAGCTGACGTAATTGTCAAAGTTGGTCTATTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACCTGAGGGAGGAGTATGAGGTTACACAACGATTTGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAGTCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTGTAGAGAATATTATATCACTATACGAGATTGTTCGTGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

379

Amino Acids

43.0

Weight (kDa)

5.88

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 155 - 325 7.3e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 860
Acc36I ACCTGC 1 cut(s) 721
AciI CCGC 1 cut(s) 676
AclWI GGATC 1 cut(s) 490
AcoI YGGCCR 1 cut(s) 697
AcsI RAATTY 1 cut(s) 989
AfiI CCNNNNNNNGG 3 cut(s) 193, 289, 940
AgsI TTSAA 6 cut(s) 85, 132, 322, 749, 821, 982
AjnI CCWGG 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 474, 506
AluBI AGCT 5 cut(s) 20, 450, 610, 814, 851
AluI AGCT 5 cut(s) 20, 450, 610, 814, 851
Alw21I GWGCWC 1 cut(s) 1002
Alw26I GTCTC 3 cut(s) 101, 202, 372
AlwI GGATC 1 cut(s) 490
AlwNI CAGNNNCTG 1 cut(s) 1133
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 6 cut(s) 170, 227, 231, 333, 632, 697
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 989
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 227, 632
AsuC2I CCSGG 2 cut(s) 226, 274
AvaI CYCGRG 1 cut(s) 192
AxyI CCTNAGG 1 cut(s) 935
BalI TGGCCA 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 1002
BbvCI CCTCAGC 1 cut(s) 606
BbvI GCAGC 1 cut(s) 29
BccI CCATC 4 cut(s) 3, 196, 565, 731
BciT130I CCWGG 1 cut(s) 454
BciVI GTATCC 1 cut(s) 683
BcnI CCSGG 2 cut(s) 226, 274
BcoDI GTCTC 3 cut(s) 101, 202, 372
BfaI CTAG 3 cut(s) 168, 536, 848
BfmI CTRYAG 1 cut(s) 1080
BfuAI ACCTGC 1 cut(s) 721
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 226, 274, 454
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 227, 632
BmrFI CCNGG 3 cut(s) 226, 274, 454
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 895, 1071
BmuI ACTGGG 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 267
Bpu10I CCTNAGC 1 cut(s) 606
BpuEI CTTGAG 2 cut(s) 17, 146
BpuMI CCSGG 2 cut(s) 226, 274
BsaBI GATNNNNATC 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 202
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 289, 940
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 4 cut(s) 93, 309, 475, 790
Bse21I CCTNAGG 1 cut(s) 935
Bse3DI GCAATG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 1 cut(s) 454
BseGI GGATG 1 cut(s) 910
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 3 cut(s) 193, 289, 940
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 3 cut(s) 406, 620, 926
BseNI ACTGG 4 cut(s) 93, 309, 475, 790
BseRI GAGGAG 3 cut(s) 20, 521, 956
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 6 cut(s) 172, 229, 233, 335, 634, 699
BsiHKAI GWGCWC 1 cut(s) 1002
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 3 cut(s) 225, 230, 273
BslI CCNNNNNNNGG 3 cut(s) 193, 289, 940
BsmAI GTCTC 3 cut(s) 101, 202, 372
BsmBI CGTCTC 1 cut(s) 372
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 6 cut(s) 172, 229, 233, 335, 634, 699
Bso31I GGTCTC 1 cut(s) 202
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 1002
Bsp143I GATC 3 cut(s) 482, 884, 1045
BspACI CCGC 1 cut(s) 676
BspANI GGCC 6 cut(s) 172, 229, 233, 335, 634, 699
BspCNI CTCAG 3 cut(s) 405, 619, 927
BspMI ACCTGC 1 cut(s) 721
BspPI GGATC 1 cut(s) 490
BspQI GCTCTTC 2 cut(s) 112, 317
BspTNI GGTCTC 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 4 cut(s) 93, 309, 475, 790
BssAI RCCGGY 1 cut(s) 229
BssMI GATC 3 cut(s) 482, 884, 1045
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 2 cut(s) 490, 1081
Bst6I CTCTTC 5 cut(s) 30, 81, 112, 317, 744
BstAPI GCANNNNNTGC 1 cut(s) 914
BstC8I GCNNGC 3 cut(s) 231, 329, 333
BstDEI CTNAG 4 cut(s) 392, 606, 650, 935
BstENI CCTNNNNNAGG 1 cut(s) 938
BstF5I GGATG 1 cut(s) 910
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 3 cut(s) 485, 887, 1048
BstMAI GTCTC 3 cut(s) 101, 202, 372
BstMBI GATC 3 cut(s) 482, 884, 1045
BstMWI GCNNNNNNNGC 3 cut(s) 239, 631, 914
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 2 cut(s) 331, 551
BstSCI CCNGG 3 cut(s) 224, 272, 452
BstSFI CTRYAG 1 cut(s) 1080
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 453
Bsu36I CCTNAGG 1 cut(s) 935
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 6 cut(s) 172, 229, 233, 335, 634, 699
BtsCI GGATG 1 cut(s) 910
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 3 cut(s) 231, 329, 333
CaiI CAGNNNCTG 1 cut(s) 1133
CfoI GCGC 1 cut(s) 242
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 2 cut(s) 227, 632
CviAII CATG 5 cut(s) 328, 463, 512, 548, 926
DdeI CTNAG 4 cut(s) 392, 606, 650, 935
DpnI GATC 3 cut(s) 484, 886, 1047
DpnII GATC 3 cut(s) 482, 884, 1045
DrdI GACNNNNNNGTC 1 cut(s) 860
DseDI GACNNNNNNGTC 1 cut(s) 860
EaeI YGGCCR 1 cut(s) 697
Eam1104I CTCTTC 5 cut(s) 30, 81, 112, 317, 744
EarI CTCTTC 5 cut(s) 30, 81, 112, 317, 744
Eco147I AGGCCT 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 202
Eco81I CCTNAGG 1 cut(s) 935
Eco88I CYCGRG 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 938
EcoRII CCWGG 1 cut(s) 452
EcoT22I ATGCAT 1 cut(s) 804
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 5 cut(s) 331, 466, 515, 551, 929
FalI AAGNNNNNCTT 4 cut(s) 535, 567, 694, 726
FatI CATG 5 cut(s) 327, 462, 511, 547, 925
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 917
FseI GGCCGGCC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 3 cut(s) 168, 536, 848
GlaI GCGC 1 cut(s) 241
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 6 cut(s) 172, 229, 233, 335, 634, 699
HapII CCGG 3 cut(s) 225, 230, 273
HhaI GCGC 1 cut(s) 242
Hin1II CATG 5 cut(s) 331, 466, 515, 551, 929
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 2 cut(s) 616, 1077
HindII GTYRAC 2 cut(s) 616, 1077
HinfI GANTC 3 cut(s) 221, 442, 1118
HpaII CCGG 3 cut(s) 225, 230, 273
Hpy166II GTNNAC 2 cut(s) 616, 1077
Hpy188I TCNGA 4 cut(s) 395, 415, 889, 1045
Hpy188III TCNNGA 5 cut(s) 34, 245, 555, 1052, 1115
Hpy8I GTNNAC 2 cut(s) 616, 1077
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 3 cut(s) 372, 420, 827
HpyCH4III ACNGT 2 cut(s) 490, 1081
HpyCH4IV ACGT 1 cut(s) 855
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 631, 914
HpyF3I CTNAG 4 cut(s) 392, 606, 650, 935
HpySE526I ACGT 1 cut(s) 855
Hsp92II CATG 5 cut(s) 331, 466, 515, 551, 929
HspAI GCGC 1 cut(s) 240
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Kzo9I GATC 3 cut(s) 482, 884, 1045
LguI GCTCTTC 2 cut(s) 112, 317
LmnI GCTCC 3 cut(s) 455, 508, 819
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 895, 1071
MaeI CTAG 3 cut(s) 168, 536, 848
MaeII ACGT 1 cut(s) 855
MaeIII GTNAC 2 cut(s) 458, 952
MalI GATC 3 cut(s) 484, 886, 1047
MboI GATC 3 cut(s) 482, 884, 1045
MfeI CAATTG 1 cut(s) 201
MhlI GDGCHC 1 cut(s) 1002
MlsI TGGCCA 1 cut(s) 699
MluCI AATT 7 cut(s) 146, 201, 316, 379, 858, 891, 989
MluNI TGGCCA 1 cut(s) 699
MmeI TCCRAC 1 cut(s) 808
Mox20I TGGCCA 1 cut(s) 699
Mph1103I ATGCAT 1 cut(s) 804
MroNI GCCGGC 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 215
MscI TGGCCA 1 cut(s) 699
MseI TTAA 4 cut(s) 288, 344, 498, 986
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 1 cut(s) 610
MspI CCGG 3 cut(s) 225, 230, 273
MspR9I CCNGG 3 cut(s) 226, 274, 454
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 1 cut(s) 454
MwoI GCNNNNNNNGC 3 cut(s) 239, 631, 914
NaeI GCCGGC 1 cut(s) 231
NciI CCSGG 2 cut(s) 226, 274
NdeII GATC 3 cut(s) 482, 884, 1045
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 5 cut(s) 331, 466, 515, 551, 929
NmeAIII GCCGAG 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 804
NspI RCATGY 2 cut(s) 331, 551
PaeI GCATGC 1 cut(s) 331
PceI AGGCCT 1 cut(s) 172
PciSI GCTCTTC 2 cut(s) 112, 317
PctI GAATGC 1 cut(s) 564
PdiI GCCGGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 3 cut(s) 221, 442, 1118
PkrI GCNGC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspPI GGNCC 2 cut(s) 227, 632
PstNI CAGNNNCTG 1 cut(s) 1133
PvuII CAGCTG 1 cut(s) 610
RigI GGCCGGCC 1 cut(s) 233
SapI GCTCTTC 2 cut(s) 112, 317
SaqAI TTAA 4 cut(s) 288, 344, 498, 986
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 482, 884, 1045
Sau96I GGNCC 2 cut(s) 227, 632
ScrFI CCNGG 3 cut(s) 226, 274, 454
SduI GDGCHC 1 cut(s) 1002
SfaNI GCATC 2 cut(s) 895, 1071
SfcI CTRYAG 1 cut(s) 1080
SmlI CTYRAG 2 cut(s) 32, 161
SmoI CTYRAG 2 cut(s) 32, 161
SphI GCATGC 1 cut(s) 331
Sse9I AATT 7 cut(s) 146, 201, 316, 379, 858, 891, 989
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 1 cut(s) 676
SspI AATATT 1 cut(s) 1090
SspMI CTAG 3 cut(s) 168, 536, 848
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 3 cut(s) 224, 272, 452
TaaI ACNGT 2 cut(s) 490, 1081
TaiI ACGT 1 cut(s) 858
TaqI TCGA 2 cut(s) 219, 769
TasI AATT 7 cut(s) 146, 201, 316, 379, 858, 891, 989
TfiI GAWTC 3 cut(s) 221, 442, 1118
Tru1I TTAA 4 cut(s) 288, 344, 498, 986
Tru9I TTAA 4 cut(s) 288, 344, 498, 986
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 4 cut(s) 273, 528, 942, 998
TspGWI ACGGA 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 938
XapI RAATTY 1 cut(s) 989
XceI RCATGY 2 cut(s) 331, 551
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 3 cut(s) 168, 536, 848
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.