MD16G1065900.v1.1

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005702
Physical Location & Seq
Reverse (-)
4613340 .. 4616345
3006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1065900.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGGGAGGTGGAGAGCCGCTGCTTCTCTTCTCCTCAGCCGCGTAGCCGCCGCCGGAGCTTATAAATCTTCTGTTCCCGCCAAGCCTCCTTTCCATCTTCACCGCCCTTCAGCCTCCACCTCCTTCCACTTCCGCTTTCTCAATTCACGACCCTTCTCTGCAATCCCGTCTCGGGTTTCCGTTGAAGCCAATGATTACGACTCTGAGCCGCCCTGTTATGCTCAGAGTCAGAGCCAAGCTAAGGAAGACGAGGAAACCGGAAAAATACCCGTTAAAGCCTACTTCCTTTGTACCAGTATCAATTTGAAGAGCATGCAAGCCGAGAATTTAAGCAATGTCATTCCTCCTTCTTCTCGCTCATCGAATTACATTGCCCTCAGATTTTGCGAAAATACTGAATTTGGAGTTTGGCGTTACATGGTCGTATTCCAGTATGGATCTGCTGTTTTGTTTAATGTTGAGGATCATGAAGTCGACGCTTATCTGGATCTAGTTACAAGACATGCTTCTGGATTACTTCCAGAGATGAGAAAAGACGATTATGCCGTAAAAGAAAAGCCACAGCTGGATGAGGACATGAAGGGTGGTCCAGATTACATAGTTCTCAAAACTTTGGACACTGATTCTATTCGCATTATTGGCAGTGTGCTTGGCCAAAGTATTGCCTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGCGGAATGGAGAAAACTGGAACTTTCACAATGCATAAAAAGAAGCTCCTCCAACTTGTTGGGAAGGCTAACTCGAACTTAGCTGATGTAATTCTTAAAGTAGGTCTTTTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTCAAATACATGAGTACCTTCGGGAGGAGTACGAGGTTACACAACGCTTCGGAAACTTGGATTTCAAGTTAAAGTTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTTCAAAACAGAAAGTCAGATCTTCTGGAATGGTGCATCATCTTCCTGTTGAGCATAGAAAACATTATATCGCTGTACGAGATTGTTCGTGATTCATCTGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

363

Amino Acids

41.12

Weight (kDa)

5.79

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 140 - 310 4.8e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 474
AccII CGCG 2 cut(s) 42, 726
AciI CCGC 9 cut(s) 18, 40, 48, 51, 78, 103, 133, 209, 726
AclWI GGATC 3 cut(s) 445, 471, 495
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 2 cut(s) 325, 398
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 4 cut(s) 292, 887, 902, 1058
AfiI CCNNNNNNNGG 3 cut(s) 172, 241, 895
AgsI TTSAA 5 cut(s) 185, 307, 704, 937, 986
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 6 cut(s) 59, 239, 565, 769, 806, 1085
AluI AGCT 6 cut(s) 59, 239, 565, 769, 806, 1085
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 445, 471, 495
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 2 cut(s) 20, 1082
ApoI RAATTY 2 cut(s) 325, 398
Asp700I GAANNNNTTC 1 cut(s) 981
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 1 cut(s) 587
BaeI ACNNNNGTAYC 2 cut(s) 869, 902
BalI TGGCCA 1 cut(s) 654
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 957
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 7
BccI CCATC 2 cut(s) 102, 686
BceAI ACGGC 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 1080
BfuAI ACCTGC 1 cut(s) 676
BglII AGATCT 1 cut(s) 1000
BisI GCNGC 7 cut(s) 18, 21, 40, 48, 51, 209, 1083
BlsI GCNGC 7 cut(s) 19, 22, 41, 49, 52, 210, 1084
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 587
BmsI GCATC 2 cut(s) 850, 1026
BpiI GAAGAC 1 cut(s) 252
Bpu10I CCTNAGC 2 cut(s) 35, 240
BsaBI GATNNNNATC 1 cut(s) 720
BsaWI WCCGGW 1 cut(s) 257
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 241, 895
Bse1I ACTGG 3 cut(s) 294, 430, 745
Bse3DI GCAATG 2 cut(s) 340, 369
Bse8I GATNNNNATC 1 cut(s) 720
BseGI GGATG 2 cut(s) 574, 865
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 3 cut(s) 172, 241, 895
BseMI GCAATG 2 cut(s) 340, 369
BseMII CTCAG 4 cut(s) 49, 195, 236, 391
BseNI ACTGG 3 cut(s) 294, 430, 745
BseRI GAGGAG 3 cut(s) 23, 761, 911
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 2 cut(s) 42, 726
BshFI GGCC 1 cut(s) 654
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 2 cut(s) 54, 258
BslI CCNNNNNNNGG 3 cut(s) 172, 241, 895
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsnI GGCC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 957
Bsp143I GATC 5 cut(s) 437, 463, 487, 839, 1000
BspACI CCGC 9 cut(s) 18, 40, 48, 51, 78, 103, 133, 209, 726
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 4 cut(s) 48, 196, 235, 390
BspFNI CGCG 2 cut(s) 42, 726
BspHI TCATGA 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 445, 471, 495
BspQI GCTCTTC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 340, 369
BsrI ACTGG 3 cut(s) 294, 430, 745
BssMI GATC 5 cut(s) 437, 463, 487, 839, 1000
Bst6I CTCTTC 3 cut(s) 33, 302, 699
BstAPI GCANNNNNTGC 1 cut(s) 869
BstC8I GCNNGC 2 cut(s) 314, 318
BstDEI CTNAG 7 cut(s) 35, 204, 222, 240, 377, 667, 802
BstENI CCTNNNNNAGG 1 cut(s) 893
BstF5I GGATG 2 cut(s) 574, 865
BstFNI CGCG 2 cut(s) 42, 726
BstKTI GATC 5 cut(s) 440, 466, 490, 842, 1003
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 5 cut(s) 437, 463, 487, 839, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 56, 639, 869
BstNSI RCATGY 2 cut(s) 316, 506
BstSFI CTRYAG 1 cut(s) 1080
BstUI CGCG 2 cut(s) 42, 726
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 3 cut(s) 437, 487, 1000
BstXI CCANNNNNNTGG 1 cut(s) 782
BstYI RGATCY 3 cut(s) 437, 487, 1000
BsuRI GGCC 1 cut(s) 654
BtsCI GGATG 2 cut(s) 574, 865
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 2 cut(s) 618, 649
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 314, 318
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 1 cut(s) 485
Csp6I GTAC 4 cut(s) 291, 886, 901, 1057
CviAII CATG 6 cut(s) 313, 418, 467, 503, 577, 881
CviQI GTAC 4 cut(s) 291, 886, 901, 1057
DdeI CTNAG 7 cut(s) 35, 204, 222, 240, 377, 667, 802
DpnI GATC 5 cut(s) 439, 465, 489, 841, 1002
DpnII GATC 5 cut(s) 437, 463, 487, 839, 1000
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 3 cut(s) 33, 302, 699
EarI CTCTTC 3 cut(s) 33, 302, 699
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 171
EcoNI CCTNNNNNAGG 1 cut(s) 893
EcoT22I ATGCAT 1 cut(s) 759
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 6 cut(s) 316, 421, 470, 506, 580, 884
FalI AAGNNNNNCTT 6 cut(s) 490, 522, 813, 845, 966, 998
FatI CATG 6 cut(s) 312, 417, 466, 502, 576, 880
FauI CCCGC 1 cut(s) 85
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 7 cut(s) 18, 21, 40, 48, 51, 209, 1083
FokI GGATG 2 cut(s) 581, 872
Fsp4HI GCNGC 7 cut(s) 18, 21, 40, 48, 51, 209, 1083
FspBI CTAG 1 cut(s) 491
GluI GCNGC 7 cut(s) 18, 21, 40, 48, 51, 209, 1083
HaeIII GGCC 1 cut(s) 654
HapII CCGG 2 cut(s) 54, 258
HgaI GACGC 1 cut(s) 485
Hin1II CATG 6 cut(s) 316, 421, 470, 506, 580, 884
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 4 cut(s) 200, 226, 623, 1073
HpaII CCGG 2 cut(s) 54, 258
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 7 cut(s) 205, 225, 231, 380, 844, 923, 1000
Hpy188III TCNNGA 9 cut(s) 147, 467, 485, 510, 521, 590, 893, 1007, 1070
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 8 cut(s) 117, 133, 163, 357, 574, 781, 899, 992
HpyCH4V TGCA 8 cut(s) 161, 316, 713, 757, 863, 973, 1017, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 639, 869
HpyF3I CTNAG 7 cut(s) 35, 204, 222, 240, 377, 667, 802
Hsp92II CATG 6 cut(s) 316, 421, 470, 506, 580, 884
Kzo9I GATC 5 cut(s) 437, 463, 487, 839, 1000
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 2 cut(s) 56, 774
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 850, 1026
MaeI CTAG 1 cut(s) 491
MaeIII GTNAC 3 cut(s) 413, 493, 907
MalI GATC 5 cut(s) 439, 465, 489, 841, 1002
MboI GATC 5 cut(s) 437, 463, 487, 839, 1000
MboII GAAGA 9 cut(s) 20, 60, 89, 257, 319, 342, 716, 995, 1015
MflI RGATCY 3 cut(s) 437, 487, 1000
MhlI GDGCHC 1 cut(s) 957
MlsI TGGCCA 1 cut(s) 654
MluCI AATT 7 cut(s) 142, 301, 325, 364, 398, 813, 846
MluNI TGGCCA 1 cut(s) 654
MlyI GAGTC 2 cut(s) 194, 235
MmeI TCCRAC 1 cut(s) 799
Mox20I TGGCCA 1 cut(s) 654
Mph1103I ATGCAT 1 cut(s) 759
MroXI GAANNNNTTC 1 cut(s) 981
MscI TGGCCA 1 cut(s) 654
MseI TTAA 6 cut(s) 273, 329, 453, 819, 941, 1087
Msp20I TGGCCA 1 cut(s) 654
MspA1I CMGCKG 2 cut(s) 20, 565
MspI CCGG 2 cut(s) 54, 258
MvnI CGCG 2 cut(s) 42, 726
MwoI GCNNNNNNNGC 3 cut(s) 56, 639, 869
NdeII GATC 5 cut(s) 437, 463, 487, 839, 1000
NlaIII CATG 6 cut(s) 316, 421, 470, 506, 580, 884
NmeAIII GCCGAG 1 cut(s) 346
NsiI ATGCAT 1 cut(s) 759
NspI RCATGY 2 cut(s) 316, 506
PaeI GCATGC 1 cut(s) 316
PagI TCATGA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 981
PfeI GAWTC 2 cut(s) 623, 1073
PkrI GCNGC 7 cut(s) 19, 22, 41, 49, 52, 210, 1084
PleI GAGTC 2 cut(s) 194, 234
PpsI GAGTC 2 cut(s) 194, 234
PsiI TTATAA 1 cut(s) 63
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 3 cut(s) 437, 487, 1000
PvuII CAGCTG 1 cut(s) 565
RsaI GTAC 4 cut(s) 292, 887, 902, 1058
RsaNI GTAC 4 cut(s) 291, 886, 901, 1057
SalI GTCGAC 1 cut(s) 473
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 6 cut(s) 273, 329, 453, 819, 941, 1087
SatI GCNGC 7 cut(s) 18, 21, 40, 48, 51, 209, 1083
Sau3AI GATC 5 cut(s) 437, 463, 487, 839, 1000
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 2 cut(s) 194, 235
SduI GDGCHC 1 cut(s) 957
SfaNI GCATC 2 cut(s) 850, 1026
SfcI CTRYAG 1 cut(s) 1080
SinI GGWCC 1 cut(s) 587
SphI GCATGC 1 cut(s) 316
Sse9I AATT 7 cut(s) 142, 301, 325, 364, 398, 813, 846
SsiI CCGC 9 cut(s) 18, 40, 48, 51, 78, 103, 133, 209, 726
SspMI CTAG 1 cut(s) 491
TaqI TCGA 3 cut(s) 362, 474, 797
TasI AATT 7 cut(s) 142, 301, 325, 364, 398, 813, 846
TauI GCSGC 5 cut(s) 20, 42, 50, 53, 211
TfiI GAWTC 2 cut(s) 623, 1073
Tru1I TTAA 6 cut(s) 273, 329, 453, 819, 941, 1087
Tru9I TTAA 6 cut(s) 273, 329, 453, 819, 941, 1087
TscAI CASTG 2 cut(s) 625, 649
TseI GCWGC 2 cut(s) 20, 1082
TspDTI ATGAA 4 cut(s) 483, 593, 953, 1065
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 2 cut(s) 625, 649
VpaK11BI GGWCC 1 cut(s) 587
XagI CCTNNNNNAGG 1 cut(s) 893
XapI RAATTY 2 cut(s) 325, 398
XceI RCATGY 2 cut(s) 316, 506
XmiI GTMKAC 1 cut(s) 474
XmnI GAANNNNTTC 1 cut(s) 981
XspI CTAG 1 cut(s) 491
Zsp2I ATGCAT 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.