RLG00000006314

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005702
Physical Location & Seq
Forward (+)
6200297 .. 6203077
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006314

Sequence Viewer

Length: 1128 bp
ATGGGGAGATGGAGAGCAGCTTCTCTTCTCCTCAAGAACATAGCCATAACCACCACACCACCCAAATCTTCTTCTCTTCTTTTCAAGCCTCTCACTCTCTCACGCTCTTCTTCTTCTTCAATCCCCACTTCCCAATTCTACTTCTTCCTCAAGTCTAGGCCTTTCTCTGCAATCCCATCTCGGGTCTCAATTGACCCGACTGATTTCGAATCCGGGCCGGCCTATTGCGTTCATGAACAACCCAATGAAGACGACGAAACCGGGAAAATCCCCATTAAGGCGTATTTCCTTTGCACCAGTATCAATTTGAAGAGCATGCAGGCCGAGTATTTAAGCTATGTCATTCCTCCTTCGTCTCGCTCAACCAATTATATTGCACTCAGATTTTGTGATTTCCCTTCGGAAAATAGTAGATTTGGAGTATGGGAGAATCCAAGCTCCTGGCGTTACATGGTAGTATTCCAGTATGGATCAACTGTTCTGTTTAATGTTGAGGAGCATGAAGTTCAGGCTTACCTAAATCTAGTCATAAGACATGCTTCTGGAATGCTACGAGAGATGGTGAAAGATGATTATGCTGTAAAAGAGAAGCCTCAGCTGGTTGACGATATGCAGGGTGGGCCAGACTACATAGTTCTTAGAACTTTGGACACGGATAGTATCCGCATTATTGGAAGTGTGCTTGGCCAAAGTATTGCTTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGAGGAATGGAAAAAACTGGAACTTTTACTATGCATAAAAAGAAGCTCCTTCAACTCGTTGGAAAGGCTAACTCAACTCTAGCTGACGTAATTGTCAAAGTTGGTCTATTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTAAAATACATGAATACCTGAGGGAGGAGTATGAGGTTACACAACGATTTGGAAACTTGGATTTCAAGTTAAAATTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTACAAAACAGAAAGTCTGATCTTCTGGAATGGTGCATCATCTTCCTGTTGACTGTAGAGAATATGATATCACTATACGAGATTGTTCGTGATTCAGGTGCAGTTTCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

376

Amino Acids

42.81

Weight (kDa)

5.89

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 151 - 321 7.1e-33 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 848
Acc36I ACCTGC 1 cut(s) 709
AciI CCGC 1 cut(s) 664
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 1 cut(s) 685
AcsI RAATTY 1 cut(s) 977
AfiI CCNNNNNNNGG 3 cut(s) 181, 277, 928
AgsI TTSAA 6 cut(s) 85, 120, 310, 737, 809, 970
AjnI CCWGG 1 cut(s) 440
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
AluBI AGCT 6 cut(s) 20, 336, 438, 598, 802, 839
AluI AGCT 6 cut(s) 20, 336, 438, 598, 802, 839
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 2 cut(s) 190, 360
AlwI GGATC 1 cut(s) 478
AlwNI CAGNNNCTG 1 cut(s) 1121
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 6 cut(s) 158, 215, 219, 321, 620, 685
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 977
AspS9I GGNCC 2 cut(s) 215, 620
AsuC2I CCSGG 2 cut(s) 214, 262
AsuHPI GGTGA 1 cut(s) 574
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 180
AxyI CCTNAGG 1 cut(s) 923
BalI TGGCCA 1 cut(s) 687
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 990
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 1 cut(s) 29
BccI CCATC 4 cut(s) 3, 184, 553, 719
BciT130I CCWGG 1 cut(s) 442
BciVI GTATCC 1 cut(s) 671
BcnI CCSGG 2 cut(s) 214, 262
BcoDI GTCTC 2 cut(s) 190, 360
BfaI CTAG 3 cut(s) 156, 524, 836
BfmI CTRYAG 1 cut(s) 1068
BfuAI ACCTGC 1 cut(s) 709
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 214, 262, 442
BmeT110I CYCGRG 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 215, 620
BmrFI CCNGG 3 cut(s) 214, 262, 442
BmsI GCATC 2 cut(s) 883, 1059
BpiI GAAGAC 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 17, 134
BpuMI CCSGG 2 cut(s) 214, 262
BsaBI GATNNNNATC 1 cut(s) 753
BsaI GGTCTC 1 cut(s) 190
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 277, 928
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 3 cut(s) 297, 463, 778
Bse21I CCTNAGG 1 cut(s) 923
Bse8I GATNNNNATC 1 cut(s) 753
BseBI CCWGG 1 cut(s) 442
BseGI GGATG 1 cut(s) 898
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 3 cut(s) 181, 277, 928
BseMII CTCAG 3 cut(s) 394, 608, 914
BseNI ACTGG 3 cut(s) 297, 463, 778
BseRI GAGGAG 3 cut(s) 20, 509, 944
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 6 cut(s) 160, 217, 221, 323, 622, 687
BsiHKAI GWGCWC 1 cut(s) 990
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 213, 218, 261
BslI CCNNNNNNNGG 3 cut(s) 181, 277, 928
BsmAI GTCTC 2 cut(s) 190, 360
BsmBI CGTCTC 1 cut(s) 360
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 6 cut(s) 160, 217, 221, 323, 622, 687
Bso31I GGTCTC 1 cut(s) 190
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 990
Bsp143I GATC 3 cut(s) 470, 872, 1033
BspACI CCGC 1 cut(s) 664
BspANI GGCC 6 cut(s) 160, 217, 221, 323, 622, 687
BspCNI CTCAG 3 cut(s) 393, 607, 915
BspHI TCATGA 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 709
BspPI GGATC 1 cut(s) 478
BspQI GCTCTTC 2 cut(s) 112, 305
BspT104I TTCGAA 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 3 cut(s) 297, 463, 778
BssAI RCCGGY 1 cut(s) 217
BssMI GATC 3 cut(s) 470, 872, 1033
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 2 cut(s) 478, 1069
Bst6I CTCTTC 5 cut(s) 30, 81, 112, 305, 732
BstAPI GCANNNNNTGC 1 cut(s) 902
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 3 cut(s) 219, 317, 321
BstDEI CTNAG 4 cut(s) 380, 594, 638, 923
BstENI CCTNNNNNAGG 1 cut(s) 926
BstF5I GGATG 1 cut(s) 898
BstKTI GATC 3 cut(s) 473, 875, 1036
BstMAI GTCTC 2 cut(s) 190, 360
BstMBI GATC 3 cut(s) 470, 872, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 619, 902
BstNI CCWGG 1 cut(s) 442
BstNSI RCATGY 2 cut(s) 319, 539
BstSCI CCNGG 3 cut(s) 212, 260, 440
BstSFI CTRYAG 1 cut(s) 1068
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 255
BstXI CCANNNNNNTGG 1 cut(s) 441
Bsu36I CCTNAGG 1 cut(s) 923
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 6 cut(s) 160, 217, 221, 323, 622, 687
BtsCI GGATG 1 cut(s) 898
BveI ACCTGC 1 cut(s) 709
Cac8I GCNNGC 3 cut(s) 219, 317, 321
CaiI CAGNNNCTG 1 cut(s) 1121
CciI TCATGA 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 215, 620
CviAII CATG 6 cut(s) 233, 316, 451, 500, 536, 914
DdeI CTNAG 4 cut(s) 380, 594, 638, 923
DpnI GATC 3 cut(s) 472, 874, 1035
DpnII GATC 3 cut(s) 470, 872, 1033
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
EaeI YGGCCR 1 cut(s) 685
Eam1104I CTCTTC 5 cut(s) 30, 81, 112, 305, 732
EarI CTCTTC 5 cut(s) 30, 81, 112, 305, 732
Eco147I AGGCCT 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 190
Eco32I GATATC 1 cut(s) 1083
Eco81I CCTNAGG 1 cut(s) 923
Eco88I CYCGRG 1 cut(s) 180
EcoNI CCTNNNNNAGG 1 cut(s) 926
EcoRII CCWGG 1 cut(s) 440
EcoRV GATATC 1 cut(s) 1083
EcoT22I ATGCAT 1 cut(s) 792
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 6 cut(s) 236, 319, 454, 503, 539, 917
FalI AAGNNNNNCTT 4 cut(s) 523, 555, 682, 714
FatI CATG 6 cut(s) 232, 315, 450, 499, 535, 913
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 1 cut(s) 905
FseI GGCCGGCC 1 cut(s) 221
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 3 cut(s) 156, 524, 836
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 6 cut(s) 160, 217, 221, 323, 622, 687
HapII CCGG 3 cut(s) 213, 218, 261
Hin1II CATG 6 cut(s) 236, 319, 454, 503, 539, 917
HincII GTYRAC 2 cut(s) 604, 1065
HindII GTYRAC 2 cut(s) 604, 1065
HinfI GANTC 3 cut(s) 209, 430, 1106
HpaII CCGG 3 cut(s) 213, 218, 261
HphI GGTGA 1 cut(s) 574
Hpy166II GTNNAC 2 cut(s) 604, 1065
Hpy188I TCNGA 4 cut(s) 383, 403, 877, 1033
Hpy188III TCNNGA 5 cut(s) 34, 233, 543, 1040, 1103
Hpy8I GTNNAC 2 cut(s) 604, 1065
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 3 cut(s) 360, 408, 815
HpyCH4III ACNGT 2 cut(s) 478, 1069
HpyCH4IV ACGT 1 cut(s) 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 619, 902
HpyF3I CTNAG 4 cut(s) 380, 594, 638, 923
HpySE526I ACGT 1 cut(s) 843
Hsp92II CATG 6 cut(s) 236, 319, 454, 503, 539, 917
KroI GCCGGC 1 cut(s) 217
KroNI GCCGGC 1 cut(s) 219
Kzo9I GATC 3 cut(s) 470, 872, 1033
LguI GCTCTTC 2 cut(s) 112, 305
LmnI GCTCC 3 cut(s) 443, 496, 807
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 883, 1059
MaeI CTAG 3 cut(s) 156, 524, 836
MaeII ACGT 1 cut(s) 843
MaeIII GTNAC 2 cut(s) 446, 940
MalI GATC 3 cut(s) 472, 874, 1035
MboI GATC 3 cut(s) 470, 872, 1033
MfeI CAATTG 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 990
MlsI TGGCCA 1 cut(s) 687
MluCI AATT 7 cut(s) 134, 189, 304, 367, 846, 879, 977
MluNI TGGCCA 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 796
Mox20I TGGCCA 1 cut(s) 687
Mph1103I ATGCAT 1 cut(s) 792
MroNI GCCGGC 1 cut(s) 217
MscI TGGCCA 1 cut(s) 687
MseI TTAA 4 cut(s) 276, 332, 486, 974
Msp20I TGGCCA 1 cut(s) 687
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 3 cut(s) 213, 218, 261
MspR9I CCNGG 3 cut(s) 214, 262, 442
MunI CAATTG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 1 cut(s) 442
MwoI GCNNNNNNNGC 2 cut(s) 619, 902
NaeI GCCGGC 1 cut(s) 219
NciI CCSGG 2 cut(s) 214, 262
NdeII GATC 3 cut(s) 470, 872, 1033
NgoMIV GCCGGC 1 cut(s) 217
NlaIII CATG 6 cut(s) 236, 319, 454, 503, 539, 917
NmeAIII GCCGAG 1 cut(s) 349
NsiI ATGCAT 1 cut(s) 792
NspI RCATGY 2 cut(s) 319, 539
NspV TTCGAA 1 cut(s) 207
PaeI GCATGC 1 cut(s) 319
PagI TCATGA 1 cut(s) 232
PceI AGGCCT 1 cut(s) 160
PciSI GCTCTTC 2 cut(s) 112, 305
PctI GAATGC 1 cut(s) 552
PdiI GCCGGC 1 cut(s) 219
PfeI GAWTC 3 cut(s) 209, 430, 1106
PkrI GCNGC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspPI GGNCC 2 cut(s) 215, 620
PstNI CAGNNNCTG 1 cut(s) 1121
PvuII CAGCTG 1 cut(s) 598
RigI GGCCGGCC 1 cut(s) 221
SapI GCTCTTC 2 cut(s) 112, 305
SaqAI TTAA 4 cut(s) 276, 332, 486, 974
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 470, 872, 1033
Sau96I GGNCC 2 cut(s) 215, 620
ScrFI CCNGG 3 cut(s) 214, 262, 442
SduI GDGCHC 1 cut(s) 990
SfaNI GCATC 2 cut(s) 883, 1059
SfcI CTRYAG 1 cut(s) 1068
SfuI TTCGAA 1 cut(s) 207
SmlI CTYRAG 2 cut(s) 32, 149
SmoI CTYRAG 2 cut(s) 32, 149
SphI GCATGC 1 cut(s) 319
Sse9I AATT 7 cut(s) 134, 189, 304, 367, 846, 879, 977
SseBI AGGCCT 1 cut(s) 160
SsiI CCGC 1 cut(s) 664
SspMI CTAG 3 cut(s) 156, 524, 836
StuI AGGCCT 1 cut(s) 160
StyD4I CCNGG 3 cut(s) 212, 260, 440
TaaI ACNGT 2 cut(s) 478, 1069
TaiI ACGT 1 cut(s) 846
TaqI TCGA 2 cut(s) 207, 757
TasI AATT 7 cut(s) 134, 189, 304, 367, 846, 879, 977
TfiI GAWTC 3 cut(s) 209, 430, 1106
Tru1I TTAA 4 cut(s) 276, 332, 486, 974
Tru9I TTAA 4 cut(s) 276, 332, 486, 974
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 6 cut(s) 221, 249, 261, 516, 930, 986
TspGWI ACGGA 1 cut(s) 668
XagI CCTNNNNNAGG 1 cut(s) 926
XapI RAATTY 1 cut(s) 977
XceI RCATGY 2 cut(s) 319, 539
XspI CTAG 3 cut(s) 156, 524, 836
Zsp2I ATGCAT 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.