FvH4_4g32250

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003528
Physical Location & Seq
Forward (+)
30971947 .. 30973270
1324 bp
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UTR
Exon/CDS
Intron
FvH4_4g32250.t2

Sequence Viewer

Length: 438 bp
ATGGGTATGCTGGACAATCTATCAGATTATTTCACAATAAGCTCCAAACCGAAAAAGAAACGCAAGGCATTGCAGACAGTTGACATCAAGGTGAAAATGGATTGTGATGGCTGTGAGAGGAGAGTTAAAAGTGCTGTTTCTAACATGAAAGGTGCTAAACAAGTTGAGGTGAACAGAAAGCAAAGCCGGGTGACGGTTACAGGCTATGTGGAAGCAAAAAAGGTGTTGAAGAGGGTGAAGAGCACCGGAAAAAGGGCCGAGATTTGGCCCTACGTTCCATACAACCTCGTTGCATACCCTTATGTCGCCGGAGCTTATGACAAGAAGGCACCGTCCGGTTACGTCAGAAACGTCCCTCAGGCATTTGCTCTCGCACCGGACGACCAGTACACCTCTATGTTTAGCGATGAAAACCCCCATGCTTGTGCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

146

Amino Acids

16.28

Weight (kDa)

9.66

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 1 - 52 7.4e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 3 cut(s) 193, 252, 264
AgsI TTSAA 1 cut(s) 229
AluBI AGCT 2 cut(s) 42, 314
AluI AGCT 2 cut(s) 42, 314
Alw21I GWGCWC 1 cut(s) 245
AoxI GGCC 2 cut(s) 255, 266
AspS9I GGNCC 2 cut(s) 255, 267
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 4 cut(s) 103, 181, 202, 247
AxyI CCTNAGG 1 cut(s) 357
BanI GGYRCC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 245
BccI CCATC 2 cut(s) 101, 437
BcnI CCSGG 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 255, 267
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 188
BsaWI WCCGGW 3 cut(s) 245, 335, 376
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 252, 264
Bse1I ACTGG 1 cut(s) 385
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 68
BseLI CCNNNNNNNGG 3 cut(s) 193, 252, 264
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 133
BshFI GGCC 2 cut(s) 257, 268
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 5 cut(s) 187, 246, 309, 336, 377
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 3 cut(s) 193, 252, 264
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 2 cut(s) 257, 268
Bsp1286I GDGCHC 1 cut(s) 245
BspANI GGCC 2 cut(s) 257, 268
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 330
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 68
BsrI ACTGG 1 cut(s) 385
Bst4CI ACNGT 3 cut(s) 79, 196, 333
Bst6I CTCTTC 2 cut(s) 224, 233
BstDEI CTNAG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 2 cut(s) 257, 268
BtgZI GCGATG 1 cut(s) 420
Cfr13I GGNCC 2 cut(s) 255, 267
Csp6I GTAC 1 cut(s) 388
CviAII CATG 3 cut(s) 145, 419, 433
CviJI RGCY 7 cut(s) 42, 111, 186, 204, 257, 268, 314
CviKI_1 RGCY 7 cut(s) 42, 111, 186, 204, 257, 268, 314
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 224, 233
EarI CTCTTC 2 cut(s) 224, 233
Eco81I CCTNAGG 1 cut(s) 357
FaeI CATG 3 cut(s) 148, 422, 436
FaqI GGGAC 1 cut(s) 338
FatI CATG 3 cut(s) 144, 418, 432
HaeIII GGCC 2 cut(s) 257, 268
HapII CCGG 5 cut(s) 187, 246, 309, 336, 377
Hin1II CATG 3 cut(s) 148, 422, 436
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HpaII CCGG 5 cut(s) 187, 246, 309, 336, 377
HphI GGTGA 4 cut(s) 103, 181, 202, 247
Hpy166II GTNNAC 3 cut(s) 82, 172, 390
Hpy188I TCNGA 2 cut(s) 25, 347
Hpy8I GTNNAC 3 cut(s) 82, 172, 390
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 3 cut(s) 79, 196, 333
HpyCH4IV ACGT 3 cut(s) 273, 342, 351
HpyCH4V TGCA 2 cut(s) 73, 293
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 3 cut(s) 273, 342, 351
Hsp92II CATG 3 cut(s) 148, 422, 436
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 2 cut(s) 47, 311
LpnPI CCDG 8 cut(s) 186, 200, 259, 322, 344, 349, 390, 398
MaeII ACGT 3 cut(s) 273, 342, 351
MaeIII GTNAC 3 cut(s) 190, 196, 338
MboII GAAGA 2 cut(s) 241, 250
MhlI GDGCHC 1 cut(s) 245
MnlI CCTC 6 cut(s) 111, 160, 225, 296, 366, 403
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 3 cut(s) 89, 395, 423
MspI CCGG 5 cut(s) 187, 246, 309, 336, 377
MspR9I CCNGG 1 cut(s) 188
NciI CCSGG 1 cut(s) 188
NlaIII CATG 3 cut(s) 148, 422, 436
NlaIV GGNNCC 1 cut(s) 330
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 190
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 378
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 255, 267
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 3 cut(s) 89, 395, 423
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 126
Sau96I GGNCC 2 cut(s) 255, 267
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 245
SmiMI CAYNNNNRTG 3 cut(s) 89, 395, 423
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 79, 196, 333
TaiI ACGT 3 cut(s) 276, 345, 354
TatI WGTACW 1 cut(s) 387
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TseFI GTSAC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 2 cut(s) 161, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.