RchiOBHm_Chr4g0441041

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003528
Physical Location & Seq
Forward (+)
62966285 .. 62967680
1396 bp
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UTR
Exon/CDS
Intron
PRQ40895

Sequence Viewer

Length: 444 bp
ATGGGCGTTCTAGACTCTCTATCAGACTATTTCACAGTGAGTCGTTCCAGACTCAGAAAGAAACGAAAGCCAATGCAGACAGTTGACATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGGAGAGTTAAGAATGCAGTCTCTCACATGAAAGGTGCAAGGCAAGTTGAAGTGAACAGAAAGCAAAGCCGGGTGACGGTGACAGGTTATTATGTGGAGCCAAACAAGGTGCTAAAGAGGGTTAAGAGGACCGGAAAGAGAGCTGAGATTTGGCCTTACGTTCCATACAACGTCGTTGCTTATCCTTACGTTGCCGGAGCTTATGACAAGAAGGCACCGTCCGGTTACATCAGGAACGTACCTCAGGCGGTTTCTACTGTGCAGGACGACGAGTACACCTCCATGTTCAGTGATGAGAATCCTCATGCTTGCGCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

147

Amino Acids

16.82

Weight (kDa)

9.73

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 31 - 86 6.1e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 334
AciI CCGC 1 cut(s) 368
AfaI GTAC 2 cut(s) 360, 395
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 1 cut(s) 170
AluBI AGCT 2 cut(s) 263, 320
AluI AGCT 2 cut(s) 263, 320
Alw26I GTCTC 1 cut(s) 145
AoxI GGCC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 1 cut(s) 249
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 3 cut(s) 106, 205, 211
AvaII GGWCC 1 cut(s) 249
AxyI CCTNAGG 1 cut(s) 363
BanI GGYRCC 1 cut(s) 334
BccI CCATC 2 cut(s) 104, 443
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 249
BmiI GGNNCC 2 cut(s) 219, 336
BmrFI CCNGG 1 cut(s) 191
BplI GAGNNNNNCTC 2 cut(s) 383, 415
BpuMI CCSGG 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 417
BsaWI WCCGGW 2 cut(s) 251, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse21I CCTNAGG 1 cut(s) 363
Bse8I GATNNNNATC 1 cut(s) 417
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 3 cut(s) 67, 255, 377
BsgI GTGCAG 1 cut(s) 401
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 1 cut(s) 334
BsiSI CCGG 4 cut(s) 190, 252, 315, 342
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 145
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 1 cut(s) 274
BspACI CCGC 1 cut(s) 368
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 3 cut(s) 66, 256, 376
BspLI GGNNCC 2 cut(s) 219, 336
BspT107I GGYRCC 1 cut(s) 334
Bst4CI ACNGT 6 cut(s) 37, 82, 107, 199, 339, 379
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 3 cut(s) 53, 264, 363
BstHHI GCGC 1 cut(s) 434
BstMAI GTCTC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 189
Bsu36I CCTNAGG 1 cut(s) 363
BsuRI GGCC 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 42, 415
Cac8I GCNNGC 1 cut(s) 430
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 249
Csp6I GTAC 2 cut(s) 359, 394
CviAII CATG 4 cut(s) 148, 403, 425, 439
CviJI RGCY 7 cut(s) 70, 114, 189, 220, 263, 274, 320
CviKI_1 RGCY 7 cut(s) 70, 114, 189, 220, 263, 274, 320
CviQI GTAC 2 cut(s) 359, 394
DdeI CTNAG 3 cut(s) 53, 264, 363
Eco47I GGWCC 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 363
FaeI CATG 4 cut(s) 151, 406, 428, 442
FaiI YATR 7 cut(s) 149, 213, 286, 324, 404, 426, 440
FatI CATG 4 cut(s) 147, 402, 424, 438
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 433
HaeIII GGCC 1 cut(s) 274
HapII CCGG 4 cut(s) 190, 252, 315, 342
HhaI GCGC 1 cut(s) 434
Hin1II CATG 4 cut(s) 151, 406, 428, 442
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 4 cut(s) 14, 40, 51, 418
HpaII CCGG 4 cut(s) 190, 252, 315, 342
HphI GGTGA 3 cut(s) 106, 205, 211
Hpy166II GTNNAC 3 cut(s) 85, 175, 396
Hpy188I TCNGA 2 cut(s) 25, 56
Hpy188III TCNNGA 3 cut(s) 11, 48, 352
Hpy8I GTNNAC 3 cut(s) 85, 175, 396
Hpy99I CGWCG 2 cut(s) 296, 392
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 6 cut(s) 37, 82, 107, 199, 339, 379
HpyCH4IV ACGT 4 cut(s) 279, 291, 309, 357
HpyCH4V TGCA 4 cut(s) 76, 137, 158, 382
HpyF3I CTNAG 3 cut(s) 53, 264, 363
HpySE526I ACGT 4 cut(s) 279, 291, 309, 357
Hsp92II CATG 4 cut(s) 151, 406, 428, 442
HspAI GCGC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 217, 317
LpnPI CCDG 9 cut(s) 61, 189, 203, 265, 328, 337, 350, 355, 368
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 279, 291, 309, 357
MaeIII GTNAC 3 cut(s) 193, 199, 344
MlyI GAGTC 3 cut(s) 8, 45, 49
MnlI CCTC 5 cut(s) 231, 240, 372, 409, 432
MseI TTAA 2 cut(s) 129, 243
MslI CAYNNNNRTG 2 cut(s) 92, 401
MspI CCGG 4 cut(s) 190, 252, 315, 342
MspR9I CCNGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 191
NlaIII CATG 4 cut(s) 151, 406, 428, 442
NlaIV GGNNCC 2 cut(s) 219, 336
NmuCI GTSAC 2 cut(s) 193, 199
PctI GAATGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 418
PleI GAGTC 3 cut(s) 8, 45, 48
PpsI GAGTC 3 cut(s) 8, 45, 48
PspN4I GGNNCC 2 cut(s) 219, 336
PspPI GGNCC 1 cut(s) 249
RsaI GTAC 2 cut(s) 360, 395
RsaNI GTAC 2 cut(s) 359, 394
RseI CAYNNNNRTG 2 cut(s) 92, 401
SaqAI TTAA 2 cut(s) 129, 243
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 3 cut(s) 8, 45, 49
ScrFI CCNGG 1 cut(s) 191
SinI GGWCC 1 cut(s) 249
SmiMI CAYNNNNRTG 2 cut(s) 92, 401
SsiI CCGC 1 cut(s) 368
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 6 cut(s) 37, 82, 107, 199, 339, 379
TaiI ACGT 4 cut(s) 282, 294, 312, 360
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 1 cut(s) 418
Tru1I TTAA 2 cut(s) 129, 243
Tru9I TTAA 2 cut(s) 129, 243
TscAI CASTG 2 cut(s) 42, 415
TseFI GTSAC 2 cut(s) 193, 199
Tsp45I GTSAC 2 cut(s) 193, 199
TspDTI ATGAA 1 cut(s) 164
TspRI CASTG 2 cut(s) 42, 415
VpaK11BI GGWCC 1 cut(s) 249
XbaI TCTAGA 1 cut(s) 10
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.