Rh4DG388700

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003528
Physical Location & Seq
Forward (+)
60516980 .. 60518495
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG388700.1

Sequence Viewer

Length: 441 bp
ATGGGCGTTCTAGACTCTCTATCAGACTATTTCACAGTGAGTCGTTCCAGACTCAGAAAGAAACGAAAGCCAATGCAGACAGTTGACATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGGAGAGTTAAGAATGCAGTCTCTCACATGAAAGGTGCAAGGCAAGTTGAAGTGAACAGAAAGCAAAGCCGGGTGACGGTGACAGGTTATGTGGAGCCAAACAAGGTGCTAAAGAGGGTTAAGAGGACCGGAAAGAGAGCTGAGATTTGGCCTTACGTTCCATACAACGTCGTTGCTTATCCTTACGTTGCCGGAGCTTATGACAAGAAGGCACCGTCCGGTTACATCAGGAACGTACCTCAGGCGGTTTCTACTGCGCAGGACGACGAGTACACCTCCATGTTCAGTGATGAGAATCCTCATGCTTGCGCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

146

Amino Acids

16.62

Weight (kDa)

9.76

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 31 - 85 2.9e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 378
AccB1I GGYRCC 1 cut(s) 331
AciI CCGC 1 cut(s) 365
AfaI GTAC 2 cut(s) 357, 392
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 1 cut(s) 170
AluBI AGCT 2 cut(s) 260, 317
AluI AGCT 2 cut(s) 260, 317
Alw26I GTCTC 1 cut(s) 145
AoxI GGCC 1 cut(s) 269
AspLEI GCGC 2 cut(s) 379, 431
AspS9I GGNCC 1 cut(s) 246
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 3 cut(s) 106, 205, 211
AvaII GGWCC 1 cut(s) 246
AxyI CCTNAGG 1 cut(s) 360
BanI GGYRCC 1 cut(s) 331
BccI CCATC 2 cut(s) 104, 440
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 216, 333
BmrFI CCNGG 1 cut(s) 191
BplI GAGNNNNNCTC 2 cut(s) 380, 412
BpuMI CCSGG 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 414
BsaWI WCCGGW 2 cut(s) 248, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse21I CCTNAGG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 414
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 3 cut(s) 67, 252, 374
BshFI GGCC 1 cut(s) 271
BshNI GGYRCC 1 cut(s) 331
BsiSI CCGG 4 cut(s) 190, 249, 312, 339
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 145
BsmI GAATGC 1 cut(s) 139
BsnI GGCC 1 cut(s) 271
BspACI CCGC 1 cut(s) 365
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 3 cut(s) 66, 253, 373
BspLI GGNNCC 2 cut(s) 216, 333
BspT107I GGYRCC 1 cut(s) 331
Bst4CI ACNGT 5 cut(s) 37, 82, 107, 199, 336
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 3 cut(s) 53, 261, 360
BstHHI GCGC 2 cut(s) 379, 431
BstMAI GTCTC 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 189
Bsu36I CCTNAGG 1 cut(s) 360
BsuRI GGCC 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 42, 412
Cac8I GCNNGC 1 cut(s) 427
CfoI GCGC 2 cut(s) 379, 431
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 2 cut(s) 356, 391
CviAII CATG 4 cut(s) 148, 400, 422, 436
CviJI RGCY 7 cut(s) 70, 114, 189, 217, 260, 271, 317
CviKI_1 RGCY 7 cut(s) 70, 114, 189, 217, 260, 271, 317
CviQI GTAC 2 cut(s) 356, 391
DdeI CTNAG 3 cut(s) 53, 261, 360
Eco47I GGWCC 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 360
FaeI CATG 4 cut(s) 151, 403, 425, 439
FaiI YATR 7 cut(s) 149, 210, 283, 321, 401, 423, 437
FatI CATG 4 cut(s) 147, 399, 421, 435
FspBI CTAG 1 cut(s) 11
FspI TGCGCA 1 cut(s) 378
GlaI GCGC 2 cut(s) 378, 430
HaeIII GGCC 1 cut(s) 271
HapII CCGG 4 cut(s) 190, 249, 312, 339
HhaI GCGC 2 cut(s) 379, 431
Hin1II CATG 4 cut(s) 151, 403, 425, 439
Hin6I GCGC 2 cut(s) 377, 429
HinP1I GCGC 2 cut(s) 377, 429
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 4 cut(s) 14, 40, 51, 415
HpaII CCGG 4 cut(s) 190, 249, 312, 339
HphI GGTGA 3 cut(s) 106, 205, 211
Hpy166II GTNNAC 3 cut(s) 85, 175, 393
Hpy188I TCNGA 2 cut(s) 25, 56
Hpy188III TCNNGA 3 cut(s) 11, 48, 349
Hpy8I GTNNAC 3 cut(s) 85, 175, 393
Hpy99I CGWCG 2 cut(s) 293, 389
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 5 cut(s) 37, 82, 107, 199, 336
HpyCH4IV ACGT 4 cut(s) 276, 288, 306, 354
HpyCH4V TGCA 3 cut(s) 76, 137, 158
HpyF3I CTNAG 3 cut(s) 53, 261, 360
HpySE526I ACGT 4 cut(s) 276, 288, 306, 354
Hsp92II CATG 4 cut(s) 151, 403, 425, 439
HspAI GCGC 2 cut(s) 377, 429
LmnI GCTCC 2 cut(s) 214, 314
LpnPI CCDG 9 cut(s) 61, 189, 203, 262, 325, 334, 347, 352, 365
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 276, 288, 306, 354
MaeIII GTNAC 3 cut(s) 193, 199, 341
MlyI GAGTC 3 cut(s) 8, 45, 49
MnlI CCTC 5 cut(s) 228, 237, 369, 406, 429
MseI TTAA 2 cut(s) 129, 240
MslI CAYNNNNRTG 2 cut(s) 92, 398
MspI CCGG 4 cut(s) 190, 249, 312, 339
MspR9I CCNGG 1 cut(s) 191
Mva1269I GAATGC 1 cut(s) 139
NciI CCSGG 1 cut(s) 191
NlaIII CATG 4 cut(s) 151, 403, 425, 439
NlaIV GGNNCC 2 cut(s) 216, 333
NmuCI GTSAC 2 cut(s) 193, 199
NsbI TGCGCA 1 cut(s) 378
PctI GAATGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 415
PleI GAGTC 3 cut(s) 8, 45, 48
PpsI GAGTC 3 cut(s) 8, 45, 48
PspN4I GGNNCC 2 cut(s) 216, 333
PspPI GGNCC 1 cut(s) 246
RsaI GTAC 2 cut(s) 357, 392
RsaNI GTAC 2 cut(s) 356, 391
RseI CAYNNNNRTG 2 cut(s) 92, 398
SaqAI TTAA 2 cut(s) 129, 240
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 3 cut(s) 8, 45, 49
ScrFI CCNGG 1 cut(s) 191
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 2 cut(s) 92, 398
SsiI CCGC 1 cut(s) 365
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 37, 82, 107, 199, 336
TaiI ACGT 4 cut(s) 279, 291, 309, 357
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 2 cut(s) 129, 240
Tru9I TTAA 2 cut(s) 129, 240
TscAI CASTG 2 cut(s) 42, 412
TseFI GTSAC 2 cut(s) 193, 199
Tsp45I GTSAC 2 cut(s) 193, 199
TspDTI ATGAA 1 cut(s) 164
TspRI CASTG 2 cut(s) 42, 412
VpaK11BI GGWCC 1 cut(s) 246
XbaI TCTAGA 1 cut(s) 10
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.