MD16G1029900.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003528
Physical Location & Seq
Reverse (-)
2145972 .. 2147422
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1029900.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGGTGCTCTGGATGCTCTTTCAGACTATTTCACAATCTCAAAACCAAGGAAGAAACGAAAGCCAATGCAGACGGTTGAAATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGGAGAGTAAAAAATGCTGTTACTAACATGAAAGGGGCTAAGCAAGTGGAGGTGAACAGAAAGCAGAGCAAAGTGACGGTGACGGGATACGTGGAGCCAAACAAGGTGTTAAAGAAAGTGAAGAGCACAGGGAAGAAGGCAGAGATTTGGCCGTACGTGCCTTACAACTTGGTGGCATATCCTTATGTCGCCGGAGCTTATGACAAGAAGGCTCCCTCCGGCTACGTGAGGAACGTGACTCAGGCACTTCCGGGCCCGAATGCGCCGGACGACAAGTATACCTCTATGTTTAGCGACGAAAATCCACATGCTTGTTCTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

147

Amino Acids

16.31

Weight (kDa)

9.62

Isoelectric Point (pI)

25.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 29 - 84 1.7e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 263
AfaI GTAC 1 cut(s) 269
AgsI TTSAA 1 cut(s) 80
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 2 cut(s) 10, 242
AoxI GGCC 2 cut(s) 263, 367
ApaI GGGCCC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 2 cut(s) 367, 368
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 3 cut(s) 100, 178, 205
BaeGI GKGCMC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 371
Bbv12I GWGCWC 2 cut(s) 10, 242
BccI CCATC 1 cut(s) 98
BceAI ACGGC 1 cut(s) 250
BciVI GTATCC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 366
BfuI GTATCC 1 cut(s) 194
BlpI GCTNAGC 1 cut(s) 153
Bme1390I CCNGG 1 cut(s) 366
BmgT120I GGNCC 2 cut(s) 367, 368
BmiI GGNNCC 3 cut(s) 210, 327, 369
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 4
Bpu1102I GCTNAGC 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 366
BsaAI YACGTR 3 cut(s) 205, 271, 340
BsaJI CCNNGG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 368
BseSI GKGCMC 1 cut(s) 371
BshFI GGCC 2 cut(s) 265, 369
BsiHKAI GWGCWC 2 cut(s) 10, 242
BsiSI CCGG 4 cut(s) 306, 333, 365, 380
BsiWI CGTACG 1 cut(s) 267
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 2 cut(s) 265, 369
Bsp120I GGGCCC 1 cut(s) 367
Bsp1286I GDGCHC 3 cut(s) 10, 242, 371
Bsp1720I GCTNAGC 1 cut(s) 153
BspANI GGCC 2 cut(s) 265, 369
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 210, 327, 369
BspQI GCTCTTC 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 47
BssNAI GTATAC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 47
Bst1107I GTATAC 1 cut(s) 393
Bst4CI ACNGT 3 cut(s) 76, 101, 193
Bst6I CTCTTC 1 cut(s) 230
BstBAI YACGTR 3 cut(s) 205, 271, 340
BstDEI CTNAG 2 cut(s) 153, 354
BstF5I GGATG 1 cut(s) 19
BstHHI GCGC 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 14, 271
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 364
BstSLI GKGCMC 1 cut(s) 371
BstZ17I GTATAC 1 cut(s) 393
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 2 cut(s) 265, 369
BtsCI GGATG 1 cut(s) 19
CfoI GCGC 1 cut(s) 379
Cfr13I GGNCC 2 cut(s) 367, 368
Csp6I GTAC 1 cut(s) 268
CviAII CATG 3 cut(s) 142, 422, 436
CviJI RGCY 9 cut(s) 64, 108, 152, 211, 265, 311, 326, 336, 369
CviKI_1 RGCY 9 cut(s) 64, 108, 152, 211, 265, 311, 326, 336, 369
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 153, 354
EaeI YGGCCR 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 3 cut(s) 145, 425, 439
FaiI YATR 8 cut(s) 143, 292, 300, 315, 393, 401, 423, 437
FatI CATG 3 cut(s) 141, 421, 435
FblI GTMKAC 1 cut(s) 392
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 371
GlaI GCGC 1 cut(s) 378
HaeIII GGCC 2 cut(s) 265, 369
HapII CCGG 4 cut(s) 306, 333, 365, 380
HhaI GCGC 1 cut(s) 379
Hin1II CATG 3 cut(s) 145, 425, 439
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HinfI GANTC 1 cut(s) 352
HpaII CCGG 4 cut(s) 306, 333, 365, 380
HphI GGTGA 3 cut(s) 100, 178, 205
Hpy166II GTNNAC 2 cut(s) 169, 393
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 1 cut(s) 11
Hpy8I GTNNAC 2 cut(s) 169, 393
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 2 cut(s) 244, 316
HpyCH4III ACNGT 3 cut(s) 76, 101, 193
HpyCH4IV ACGT 4 cut(s) 204, 270, 339, 348
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 271
HpyF3I CTNAG 2 cut(s) 153, 354
HpySE526I ACGT 4 cut(s) 204, 270, 339, 348
Hsp92II CATG 3 cut(s) 145, 425, 439
HspAI GCGC 1 cut(s) 377
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 3 cut(s) 208, 308, 331
LpnPI CCDG 6 cut(s) 228, 319, 341, 346, 378, 393
LweI GCATC 1 cut(s) 4
MaeII ACGT 4 cut(s) 204, 270, 339, 348
MaeIII GTNAC 4 cut(s) 133, 187, 193, 349
MboII GAAGA 3 cut(s) 64, 247, 259
MhlI GDGCHC 3 cut(s) 10, 242, 371
MlyI GAGTC 1 cut(s) 346
MnlI CCTC 4 cut(s) 157, 336, 340, 406
MseI TTAA 1 cut(s) 224
MspI CCGG 4 cut(s) 306, 333, 365, 380
MspR9I CCNGG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 379
MwoI GCNNNNNNNGC 2 cut(s) 14, 271
NciI CCSGG 1 cut(s) 366
NlaIII CATG 3 cut(s) 145, 425, 439
NlaIV GGNNCC 3 cut(s) 210, 327, 369
NmuCI GTSAC 3 cut(s) 187, 193, 349
NspI RCATGY 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 379
Pfl23II CGTACG 1 cut(s) 267
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
Ppu21I YACGTR 3 cut(s) 205, 271, 340
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 3 cut(s) 210, 327, 369
PspOMI GGGCCC 1 cut(s) 367
PspPI GGNCC 2 cut(s) 367, 368
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 224
Sau96I GGNCC 2 cut(s) 367, 368
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 3 cut(s) 10, 242, 371
SetI ASST 9 cut(s) 90, 168, 207, 222, 273, 313, 342, 351, 398
SfaNI GCATC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 3 cut(s) 76, 101, 193
TaiI ACGT 4 cut(s) 207, 273, 342, 351
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseFI GTSAC 3 cut(s) 187, 193, 349
Tsp45I GTSAC 3 cut(s) 187, 193, 349
TspDTI ATGAA 1 cut(s) 158
XceI RCATGY 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.