Rw4G032950

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003528
Physical Location & Seq
Forward (+)
60014545 .. 60015717
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032950.1

Sequence Viewer

Length: 438 bp
ATGGGCGTTCTAGACTCTCTATCAGACTATTTCACAGTGAGTTCCAGACTCAGAAAGAAACGAAAGCCAATGCAGACAGTTGACATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGGAGAGTTAAGAATGCGGTCTCTCATATGAAAGGTGCAAAGCAAGTTGAAGTGAACAGAAAGCAAAGCCGGGTGACGGTGACAGGTTATGTGGAGCCAAACAAGGTGCTAAAGAGGGTTAAGAGGACCGGAAAGAGAGCTGAGATTTGGCCTTACGTTCCATACAACGTCGTTGCTTATCCTTACGTTGCCGGAGCTTATGACAAGAAGGCACCGTCCGGTTACATCAGGAACGTACCTCAGGCGGTTTCTACTGCACAGGACGACGAGTACACCTCCATGTTCAGTGATGAGAATCCTCATGCTTGCGCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

145

Amino Acids

16.44

Weight (kDa)

9.66

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 84 2.4e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AciI CCGC 2 cut(s) 134, 362
AfaI GTAC 2 cut(s) 354, 389
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 1 cut(s) 167
AluBI AGCT 2 cut(s) 257, 314
AluI AGCT 2 cut(s) 257, 314
Alw26I GTCTC 1 cut(s) 142
AoxI GGCC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 243
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 3 cut(s) 103, 202, 208
AvaII GGWCC 1 cut(s) 243
AxyI CCTNAGG 1 cut(s) 357
BanI GGYRCC 1 cut(s) 328
BccI CCATC 2 cut(s) 101, 437
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 213, 330
BmrFI CCNGG 1 cut(s) 188
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpuMI CCSGG 1 cut(s) 188
BsaBI GATNNNNATC 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 142
BsaWI WCCGGW 2 cut(s) 245, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse21I CCTNAGG 1 cut(s) 357
Bse8I GATNNNNATC 1 cut(s) 411
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 3 cut(s) 64, 249, 371
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 328
BsiSI CCGG 4 cut(s) 187, 246, 309, 336
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 1 cut(s) 268
Bso31I GGTCTC 1 cut(s) 142
BspACI CCGC 2 cut(s) 134, 362
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 3 cut(s) 63, 250, 370
BspLI GGNNCC 2 cut(s) 213, 330
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 142
Bst4CI ACNGT 5 cut(s) 37, 79, 104, 196, 333
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 3 cut(s) 50, 258, 357
BstHHI GCGC 1 cut(s) 428
BstMAI GTCTC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 186
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 42, 409
Cac8I GCNNGC 1 cut(s) 424
CfoI GCGC 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 243
Csp6I GTAC 2 cut(s) 353, 388
CviAII CATG 3 cut(s) 397, 419, 433
CviJI RGCY 7 cut(s) 67, 111, 186, 214, 257, 268, 314
CviKI_1 RGCY 7 cut(s) 67, 111, 186, 214, 257, 268, 314
CviQI GTAC 2 cut(s) 353, 388
DdeI CTNAG 3 cut(s) 50, 258, 357
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 243
Eco81I CCTNAGG 1 cut(s) 357
FaeI CATG 3 cut(s) 400, 422, 436
FaiI YATR 8 cut(s) 144, 146, 207, 280, 318, 398, 420, 434
FatI CATG 3 cut(s) 396, 418, 432
FauNDI CATATG 1 cut(s) 144
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 427
HaeIII GGCC 1 cut(s) 268
HapII CCGG 4 cut(s) 187, 246, 309, 336
HhaI GCGC 1 cut(s) 428
Hin1II CATG 3 cut(s) 400, 422, 436
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 3 cut(s) 14, 48, 412
HpaII CCGG 4 cut(s) 187, 246, 309, 336
HphI GGTGA 3 cut(s) 103, 202, 208
Hpy166II GTNNAC 3 cut(s) 82, 172, 390
Hpy188I TCNGA 2 cut(s) 25, 53
Hpy188III TCNNGA 3 cut(s) 11, 45, 346
Hpy8I GTNNAC 3 cut(s) 82, 172, 390
Hpy99I CGWCG 2 cut(s) 290, 386
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 5 cut(s) 37, 79, 104, 196, 333
HpyCH4IV ACGT 4 cut(s) 273, 285, 303, 351
HpyCH4V TGCA 3 cut(s) 73, 155, 374
HpyF3I CTNAG 3 cut(s) 50, 258, 357
HpySE526I ACGT 4 cut(s) 273, 285, 303, 351
Hsp92II CATG 3 cut(s) 400, 422, 436
HspAI GCGC 1 cut(s) 426
LmnI GCTCC 2 cut(s) 211, 311
LpnPI CCDG 9 cut(s) 58, 186, 200, 259, 322, 331, 344, 349, 362
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 273, 285, 303, 351
MaeIII GTNAC 3 cut(s) 190, 196, 338
MlyI GAGTC 2 cut(s) 8, 42
MnlI CCTC 5 cut(s) 225, 234, 366, 403, 426
MseI TTAA 2 cut(s) 126, 237
MslI CAYNNNNRTG 2 cut(s) 89, 395
MspI CCGG 4 cut(s) 187, 246, 309, 336
MspR9I CCNGG 1 cut(s) 188
Mva1269I GAATGC 1 cut(s) 136
NciI CCSGG 1 cut(s) 188
NdeI CATATG 1 cut(s) 144
NlaIII CATG 3 cut(s) 400, 422, 436
NlaIV GGNNCC 2 cut(s) 213, 330
NmuCI GTSAC 2 cut(s) 190, 196
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 412
PleI GAGTC 2 cut(s) 8, 42
PpsI GAGTC 2 cut(s) 8, 42
PspN4I GGNNCC 2 cut(s) 213, 330
PspPI GGNCC 1 cut(s) 243
RsaI GTAC 2 cut(s) 354, 389
RsaNI GTAC 2 cut(s) 353, 388
RseI CAYNNNNRTG 2 cut(s) 89, 395
SaqAI TTAA 2 cut(s) 126, 237
Sau96I GGNCC 1 cut(s) 243
SchI GAGTC 2 cut(s) 8, 42
ScrFI CCNGG 1 cut(s) 188
SinI GGWCC 1 cut(s) 243
SmiMI CAYNNNNRTG 2 cut(s) 89, 395
SsiI CCGC 2 cut(s) 134, 362
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 186
TaaI ACNGT 5 cut(s) 37, 79, 104, 196, 333
TaiI ACGT 4 cut(s) 276, 288, 306, 354
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 412
Tru1I TTAA 2 cut(s) 126, 237
Tru9I TTAA 2 cut(s) 126, 237
TscAI CASTG 2 cut(s) 42, 409
TseFI GTSAC 2 cut(s) 190, 196
Tsp45I GTSAC 2 cut(s) 190, 196
TspDTI ATGAA 1 cut(s) 161
TspRI CASTG 2 cut(s) 42, 409
VpaK11BI GGWCC 1 cut(s) 243
XbaI TCTAGA 1 cut(s) 10
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.